MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 240)


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+11

Accession Score Description
1 DNAK_PSEPK 321 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+12

Accession Score Description
1 DBHB_PSEAE 296 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+13

Accession Score Description
1 EFG1_PSEPK 288 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

-14

Accession Score Description
1 HTPG_PSEPK 268 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1
Score Mass Matches Sequences emPAI
14.1 HTPG_PSEPK 268 71567 12 (4) 11 (4) 0.13
Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

-12 peptide matches (12 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
269   321.7196 641.4246 641.4224 3.47 0 27 0.31 +1Score > 35 indicates identity K.IAGLLR.F
344   339.7015 677.3884 677.3860 3.54 0 29 0.053 +1Score > 40 indicates identity
Score > 29 indicates homology
K.LYVQR.V
482   744.4554 743.4481 743.4429 7.05 1 3 1.1 +2Score > 40 indicates identity
Score > 16 indicates homology
U K.LLVELSA.-
584   396.2261 790.4376 790.4337 4.96 0 31 0.39 +1Score > 41 indicates identity
Score > 40 indicates homology
U K.AFVDIAR.G
1016   487.2712 972.5278 972.5240 3.97 1 26 0.87 +1Score > 42 indicates identity
Score > 38 indicates homology
U R.QILEASGQK.V
1219   537.2841 1072.5536 1072.5513 2.20 1 35 0.071 +1Score > 42 indicates identity
Score > 36 indicates homology
U K.GALGDSVAEVR.V
2004   719.4000 1436.7854 1436.7663 13.3 1 83 3.9e-007 +1Score > 37 indicates identity
Score > 31 indicates homology
U K.LEYNSLLYVPAR.A
2066   737.8823 1473.7500 1473.7423 5.23 0 127 1.3e-010 +1Score > 41 indicates identity U K.GVVDSNDLSLNVSR.E
2102   498.9546 1493.8420 1493.8242 11.9 0 23 0.052 +1Score > 35 indicates identity
Score > 23 indicates homology
U K.YSDHIALPIQLPK.E
3326   714.3866 2140.1380 2140.1198 8.50 1 9 0.6 +1Score > 37 indicates identity
Score > 19 indicates homology
U R.LTDSPAILAIGEQDLGLQMR.Q
3327   1071.5778 2141.1410 2141.1038 17.4 1 11 0.38 +1Score > 37 indicates identity
Score > 19 indicates homology
U R.LTDSPAILAIGEQDLGLQMR.Q + Deamidated (NQ)
3541   1123.0332 2244.0518 2244.0182 15.0 1 131 8.1e-012 +1Score > 39 indicates identity
Score > 33 indicates homology
U R.FASTQDDSGEQSVALADYLAR.A + Deamidated (NQ)

1 subset or intersection (12 subset proteins in total)

Score Mass Subset of
HTPG_HAHCH 127 73307 14.1
Chaperone protein htpG OS=Hahella chejuensis (strain KCTC 2396) GN=htpG PE=3 SV=1
11 samesets of HTPG_HAHCH
HTPG_PSEAE 127 71624
Chaperone protein htpG OS=Pseudomonas aeruginosa GN=htpG PE=3 SV=1
HTPG_PSEMY 127 71459
Chaperone protein htpG OS=Pseudomonas mendocina (strain ymp) GN=htpG PE=3 SV=1
HTPG_PSEPF 127 71241
Chaperone protein htpG OS=Pseudomonas fluorescens (strain Pf0-1) GN=htpG PE=3 SV=1
HTPG_PSEA7 127 71511
Chaperone protein htpG OS=Pseudomonas aeruginosa (strain PA7) GN=htpG PE=3 SV=1
HTPG_PSEF5 127 71305
Chaperone protein htpG OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=htpG PE=3 SV=1
HTPG_PSEFS 127 71589
Chaperone protein htpG OS=Pseudomonas fluorescens (strain SBW25) GN=htpG PE=3 SV=1
HTPG_PSESM 127 71431
Chaperone protein htpG OS=Pseudomonas syringae pv. tomato GN=htpG PE=3 SV=1
HTPG_PSEU2 127 71288
Chaperone protein htpG OS=Pseudomonas syringae pv. syringae (strain B728a) GN=htpG PE=3 SV=2
HTPG_PSE14 127 71373
Chaperone protein htpG OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=htpG PE=3 SV=1
HTPG_MARMS 127 73100
Chaperone protein htpG OS=Marinomonas sp. (strain MWYL1) GN=htpG PE=3 SV=1
HTPG_SACD2 127 72361
Chaperone protein htpG OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=htpG PE=3 SV=1

+15

Accession Score Description
1 SUCC_PSEPK 266 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+16

Accession Score Description
1 DHSA_ECOLI 256 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+17

Accession Score Description
1 RISB_PSEPG 254 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+18

Accession Score Description
1 ACON2_PSEAE 253 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+19

Accession Score Description
1 RS5_PSEE4 226 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+20

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSEAE 211 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1
2 PORF_PSESY 177 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
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