MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 240)


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+131

Accession Score Description
1 Y1199_THISH 47 UPF0341 protein Tgr7_1199 OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=Tgr7_1199 PE=3 SV=1

+132

Accession Score Description
1 RS8_PSEP1 46 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1

+133

Accession Score Description
1 DADA_PSEPG 46 D-amino acid dehydrogenase small subunit OS=Pseudomonas putida (strain GB-1) GN=dadA PE=3 SV=1

+134

Accession Score Description
1 GUAA_PSEE4 34 GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas entomophila (strain L48) GN=guaA PE=3 SV=1

+135

Accession Score Description
1 LEUC_PSEF5 45 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1

+136

Accession Score Description
1 KIF15_HUMAN 45 Kinesin-like protein KIF15 OS=Homo sapiens GN=KIF15 PE=1 SV=1

+137

Accession Score Description
1 PROB_ACIBC 45 Glutamate 5-kinase OS=Acinetobacter baumannii (strain ACICU) GN=proB PE=3 SV=1

-138

Accession Score Description
1 ARGJ_OCEIH 45 Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1
Score Mass Matches Sequences emPAI
138.1 ARGJ_OCEIH 45 44036 1 (1) 1 (1) 0.05
Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
625   407.2784 812.5422 812.5371 6.30 1 45 0.0022 +1Score > 31 indicates identity U K.LIEVIVK.G

+139

Accession Score Description
Family member distances as a dendrogram 1 IDH_ECOLI 45 Isocitrate dehydrogenase [NADP] OS=Escherichia coli (strain K12) GN=icd PE=1 SV=1
2 IDH1_COLMA 43 Isocitrate dehydrogenase [NADP] 1 OS=Colwellia maris GN=icdI PE=1 SV=2

+140

Accession Score Description
1 DADA_VIBVU 45 D-amino acid dehydrogenase small subunit OS=Vibrio vulnificus GN=dadA PE=3 SV=1
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