MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 240)


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+131

Accession Score Description
1 Y1199_THISH 47 UPF0341 protein Tgr7_1199 OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=Tgr7_1199 PE=3 SV=1

+132

Accession Score Description
1 RS8_PSEP1 46 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1

+133

Accession Score Description
1 DADA_PSEPG 46 D-amino acid dehydrogenase small subunit OS=Pseudomonas putida (strain GB-1) GN=dadA PE=3 SV=1

-134

Accession Score Description
1 GUAA_PSEE4 34 GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas entomophila (strain L48) GN=guaA PE=3 SV=1
Score Mass Matches Sequences emPAI
134.1 GUAA_PSEE4 34 58412 4 (2) 4 (2) 0.08
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas entomophila (strain L48) GN=guaA PE=3 SV=1
4 samesets of GUAA_PSEE4
GUAA_PSEPW 34 58459 4 (2) 4 (2) 0.08
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain W619) GN=guaA PE=3 SV=1
GUAA_PSEPG 34 58496 4 (2) 4 (2) 0.08
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain GB-1) GN=guaA PE=3 SV=1
GUAA_PSEPK 34 58510 4 (2) 4 (2) 0.08
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain KT2440) GN=guaA PE=3 SV=1
GUAA_PSEP1 34 58452 4 (2) 4 (2) 0.08
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=guaA PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
225   308.6876 615.3606 615.3592 2.37 0 19 1.2 +5Score > 45 indicates identity
Score > 32 indicates homology
U R.VDVVGK.S
1319   565.3182 1128.6218 1128.6139 7.08 1 46 0.019 +1Score > 41 indicates identity U R.IINEIDGISR.V
1822   676.3751 1350.7356 1350.7184 12.8 0 3 3.4 +7Score > 38 indicates identity
Score > 21 indicates homology
U K.TSQAFVVFQPVK.S + Deamidated (NQ)
2790   911.4542 1820.8938 1820.8832 5.83 1 17 0.043 +1Score > 40 indicates identity
Score > 16 indicates homology
U R.VTYDVSSKPPATIEWE.-

2 subsets and intersections (32 subset proteins in total)

Score Mass Subset of
GUAA_AERS4 46 58989 134.1
GMP synthase [glutamine-hydrolyzing] OS=Aeromonas salmonicida (strain A449) GN=guaA PE=3 SV=1
17 samesets of GUAA_AERS4
GUAA_AERHH 46 59253
GMP synthase [glutamine-hydrolyzing] OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=guaA PE=3 SV=1
GUAA_ALTMD 46 58946
GMP synthase [glutamine-hydrolyzing] OS=Alteromonas macleodii (strain DSM 17117 / Deep ecotype) GN=guaA PE=3 SV=1
GUAA_BAUCH 46 59890
GMP synthase [glutamine-hydrolyzing] OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=guaA PE=3 SV=1
GUAA_PSEHT 46 59019
GMP synthase [glutamine-hydrolyzing] OS=Pseudoalteromonas haloplanktis (strain TAC 125) GN=guaA PE=3 SV=1
GUAA_PSYIN 46 59045
GMP synthase [glutamine-hydrolyzing] OS=Psychromonas ingrahamii (strain 37) GN=guaA PE=3 SV=1
GUAA_SHELP 46 58557
GMP synthase [glutamine-hydrolyzing] OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=guaA PE=3 SV=1
GUAA_SHEPW 46 58598
GMP synthase [glutamine-hydrolyzing] OS=Shewanella piezotolerans (strain WP3 / JCM 13877) GN=guaA PE=3 SV=1
GUAA_SHESA 46 58673
GMP synthase [glutamine-hydrolyzing] OS=Shewanella sp. (strain ANA-3) GN=guaA PE=3 SV=1
GUAA_SHESH 46 58863
GMP synthase [glutamine-hydrolyzing] OS=Shewanella sediminis (strain HAW-EB3) GN=guaA PE=3 SV=1
GUAA_SHESM 46 58673
GMP synthase [glutamine-hydrolyzing] OS=Shewanella sp. (strain MR-4) GN=guaA PE=3 SV=1
GUAA_SHESR 46 58730
GMP synthase [glutamine-hydrolyzing] OS=Shewanella sp. (strain MR-7) GN=guaA PE=3 SV=1
GUAA_SHEWM 46 58844
GMP synthase [glutamine-hydrolyzing] OS=Shewanella woodyi (strain ATCC 51908 / MS32) GN=guaA PE=3 SV=1
GUAA_SODGM 46 58966
GMP synthase [glutamine-hydrolyzing] OS=Sodalis glossinidius (strain morsitans) GN=guaA PE=3 SV=1
GUAA_TOLAT 46 59232
GMP synthase [glutamine-hydrolyzing] OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=guaA PE=3 SV=1
GUAA_COLP3 46 58540
GMP synthase [glutamine-hydrolyzing] OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=guaA PE=3 SV=1
GUAA_PSEA6 46 58909
GMP synthase [glutamine-hydrolyzing] OS=Pseudoalteromonas atlantica (strain T6c / BAA-1087) GN=guaA PE=3 SV=1
GUAA_ENTS8 46 59077
GMP synthase [glutamine-hydrolyzing] OS=Enterobacter sakazakii (strain ATCC BAA-894) GN=guaA PE=3 SV=1
GUAA_MARMS 17 58609 134.1
GMP synthase [glutamine-hydrolyzing] OS=Marinomonas sp. (strain MWYL1) GN=guaA PE=3 SV=1
13 samesets of GUAA_MARMS
GUAA_PSEAB 17 58251
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=guaA PE=3 SV=1
GUAA_PSEAE 17 58265
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas aeruginosa GN=guaA PE=3 SV=1
GUAA_PSEA8 17 58265
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas aeruginosa (strain LESB58) GN=guaA PE=3 SV=1
GUAA_PSEMY 17 58194
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas mendocina (strain ymp) GN=guaA PE=3 SV=1
GUAA_PSEFS 17 58797
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas fluorescens (strain SBW25) GN=guaA PE=3 SV=1
GUAA_PSEPF 17 58642
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas fluorescens (strain Pf0-1) GN=guaA PE=3 SV=1
GUAA_PSESM 17 58536
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas syringae pv. tomato GN=guaA PE=3 SV=1
GUAA_PSEU2 17 58491
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas syringae pv. syringae (strain B728a) GN=guaA PE=3 SV=1
GUAA_PSE14 17 58563
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=guaA PE=3 SV=1
GUAA_PSEF5 17 58754
GMP synthase [glutamine-hydrolyzing] OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=guaA PE=3 SV=1
GUAA_PSYA2 17 59049
GMP synthase [glutamine-hydrolyzing] OS=Psychrobacter arcticus (strain DSM 17307 / 273-4) GN=guaA PE=3 SV=1
GUAA_METPP 17 58810
GMP synthase [glutamine-hydrolyzing] OS=Methylibium petroleiphilum (strain PM1) GN=guaA PE=3 SV=1
GUAA_RHOFD 17 59272
GMP synthase [glutamine-hydrolyzing] OS=Rhodoferax ferrireducens (strain DSM 15236 / ATCC BAA-621 / T118) GN=guaA PE=3 SV=1

+135

Accession Score Description
1 LEUC_PSEF5 45 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1

+136

Accession Score Description
1 KIF15_HUMAN 45 Kinesin-like protein KIF15 OS=Homo sapiens GN=KIF15 PE=1 SV=1

+137

Accession Score Description
1 PROB_ACIBC 45 Glutamate 5-kinase OS=Acinetobacter baumannii (strain ACICU) GN=proB PE=3 SV=1

+138

Accession Score Description
1 ARGJ_OCEIH 45 Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1

+139

Accession Score Description
Family member distances as a dendrogram 1 IDH_ECOLI 45 Isocitrate dehydrogenase [NADP] OS=Escherichia coli (strain K12) GN=icd PE=1 SV=1
2 IDH1_COLMA 43 Isocitrate dehydrogenase [NADP] 1 OS=Colwellia maris GN=icdI PE=1 SV=2

+140

Accession Score Description
1 DADA_VIBVU 45 D-amino acid dehydrogenase small subunit OS=Vibrio vulnificus GN=dadA PE=3 SV=1
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