MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 240)


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+121

Accession Score Description
1 DNAB_MYCPN 50 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+122

Accession Score Description
1 GLNA_RHOCA 49 Glutamine synthetase (Fragment) OS=Rhodobacter capsulatus GN=glnA PE=3 SV=2

+123

Accession Score Description
1 TYPH_AZOC5 49 Putative thymidine phosphorylase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=AZC_2467 PE=3 SV=1

+124

Accession Score Description
1 BETI_PSEFS 49 HTH-type transcriptional regulator betI OS=Pseudomonas fluorescens (strain SBW25) GN=betI PE=3 SV=1

+125

Accession Score Description
1 ISPD_ALKMQ 48 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1

+126

Accession Score Description
1 NUOCD_PSEPK 47 NADH-quinone oxidoreductase subunit C/D OS=Pseudomonas putida (strain KT2440) GN=nuoC PE=3 SV=1

+127

Accession Score Description
1 CAPA_PSEFR 47 Cold shock protein capA (Fragment) OS=Pseudomonas fragi GN=capA PE=2 SV=1

+128

Accession Score Description
1 V51K_BPL79 47 51.5 kDa protein OS=Lactococcus phage (isolate 7-9) PE=4 SV=1

+129

Accession Score Description
1 ODP1_MYCTU 47 Pyruvate dehydrogenase E1 component OS=Mycobacterium tuberculosis GN=aceE PE=3 SV=1

-130

Accession Score Description
1 NFRA_STAAB 47 NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) GN=nfrA PE=3 SV=1
Score Mass Matches Sequences emPAI
130.1 NFRA_STAAB 47 28605 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) GN=nfrA PE=3 SV=1
15 samesets of NFRA_STAAB
NFRA_STAAC 47 28579 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain COL) GN=nfrA PE=3 SV=1
NFRA_STAAM 47 28591 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=nfrA PE=1 SV=1
NFRA_STAAN 47 28591 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain N315) GN=nfrA PE=3 SV=1
NFRA_STAAR 47 28534 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain MRSA252) GN=nfrA PE=3 SV=1
NFRA_STAAS 47 28578 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain MSSA476) GN=nfrA PE=3 SV=1
NFRA_STAAW 47 28578 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain MW2) GN=nfrA PE=3 SV=1
RDRP_ACLSA 47 219105 4 (1) 1 (1) 0.01
RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1
RDRP_ACLSP 47 219160 4 (1) 1 (1) 0.01
RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate plum P863) PE=4 SV=1
RPOC2_PINTH 47 140153 4 (1) 1 (1) 0.02
DNA-directed RNA polymerase subunit beta'' OS=Pinus thunbergii GN=rpoC2 PE=3 SV=2
DNM3L_HUMAN 47 44611 4 (1) 1 (1) 0.05
DNA (cytosine-5)-methyltransferase 3-like OS=Homo sapiens GN=DNMT3L PE=1 SV=2
DNM3L_MOUSE 47 49159 4 (1) 1 (1) 0.05
DNA (cytosine-5)-methyltransferase 3-like OS=Mus musculus GN=Dnmt3l PE=1 SV=1
DNM3L_RAT 47 49388 4 (1) 1 (1) 0.05
DNA (cytosine-5)-methyltransferase 3-like OS=Rattus norvegicus GN=Dnmt3l PE=2 SV=1
NFRA_STAA3 47 28579 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain USA300) GN=nfrA PE=3 SV=1
NRFA_STAA8 47 28579 4 (1) 1 (1) 0.08
NADPH-dependent oxidoreductase OS=Staphylococcus aureus (strain NCTC 8325) GN=nfrA PE=1 SV=1
UN84B_MOUSE 47 78318 4 (1) 1 (1) 0.03
Protein unc-84 homolog B OS=Mus musculus GN=Unc84b PE=1 SV=2

-4 peptide matches (2 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
331 +2 337.2044 672.3942 672.3919 3.55 0 47 0.014 +1Score > 41 indicates identity U K.SGLIQR.-
332   673.4017 672.3944 672.3919 3.81 0 21 2 +7Score > 41 indicates identity
Score > 36 indicates homology
U K.SGLIQR.-

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