MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 111–120 (out of 240)


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+111

Accession Score Description
1 RS3_PSEE4 52 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1

+112

Accession Score Description
1 THIO_PSEAE 52 Thioredoxin OS=Pseudomonas aeruginosa GN=trxA PE=3 SV=1

+113

Accession Score Description
1 RS18_PSEE4 52 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+114

Accession Score Description
1 POLG_HAVNO 51 Genome polyprotein OS=Human hepatitis A virus genotype IIIA (isolate NOR-21) PE=3 SV=4

-115

Accession Score Description
1 ASPA_PSEFL 51 Aspartate ammonia-lyase OS=Pseudomonas fluorescens GN=aspA PE=3 SV=1
Score Mass Matches Sequences emPAI
115.1 ASPA_PSEFL 51 51704 1 (1) 1 (1) 0.04
Aspartate ammonia-lyase OS=Pseudomonas fluorescens GN=aspA PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3833   817.8109 2450.4109 2450.3784 13.2 0 51 2.4e-005 +1Score > 28 indicates identity
Score > 17 indicates homology
U R.LGLLLGHDALLASLDSLIQAFAAK.G + Deamidated (NQ)

+116

Accession Score Description
1 DHQS_METLZ 51 3-dehydroquinate synthase OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=Mlab_1653 PE=3 SV=1

+117

Accession Score Description
1 RL16_PSEF5 50 50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplP PE=3 SV=1

+118

Accession Score Description
1 RPOB_OLTVI 50 DNA-directed RNA polymerase subunit beta OS=Oltmannsiellopsis viridis GN=rpoB PE=3 SV=1

+119

Accession Score Description
1 RRAA_PSEPG 50 Regulator of ribonuclease activity A OS=Pseudomonas putida (strain GB-1) GN=rraA PE=3 SV=1

+120

Accession Score Description
1 CYOB_PSEPU 50 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1
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