MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 111–120 (out of 240)


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-111

Accession Score Description
1 RS3_PSEE4 52 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
Score Mass Matches Sequences emPAI
111.1 RS3_PSEE4 52 25709 18 (2) 9 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
3 samesets of RS3_PSEE4
RS3_PSEP1 52 25709 18 (2) 9 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsC PE=3 SV=1
RS3_PSEPK 52 25709 18 (2) 9 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2
RS3_PSEU5 52 25730 18 (2) 9 (2) 0.19
30S ribosomal protein S3 OS=Pseudomonas stutzeri (strain A1501) GN=rpsC PE=3 SV=1

-18 peptide matches (15 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
303   659.3859 658.3786 658.3762 3.65 0 35 0.26 +1Score > 42 indicates identity K.IQVSGR.L
304   330.1967 658.3788 658.3762 3.99 0 32 0.16 +1Score > 42 indicates identity
Score > 36 indicates homology
K.IQVSGR.L
370   346.7122 691.4098 691.4057 5.95 0 18 0.13 +1Score > 37 indicates identity
Score > 22 indicates homology
K.VWIFK.G
495   377.6812 753.3478 753.3446 4.35 1 27 0.076 +1Score > 39 indicates identity
Score > 29 indicates homology
R.TEWYR.E
566   393.7285 785.4424 785.4395 3.72 1 26 0.84 +1Score > 43 indicates identity
Score > 38 indicates homology
R.LGGAEIAR.T
576   395.2063 788.3980 788.3963 2.23 0 18 0.31 +1Score > 43 indicates identity
Score > 25 indicates homology
R.AVQNAMR.I
577   789.4059 788.3986 788.3963 2.97 0 26 0.49 +1Score > 44 indicates identity
Score > 35 indicates homology
R.AVQNAMR.I
591   795.3909 794.3836 794.3810 3.29 1 32 0.47 +1Score > 41 indicates identity R.EYLQDK.L
593   398.1999 794.3852 794.3810 5.33 1 14 0.63 +1Score > 41 indicates identity
Score > 25 indicates homology
R.EYLQDK.L
1666 +1 320.1834 1276.7045 1276.7000 3.52 0 10 0.67 +1Score > 38 indicates identity
Score > 21 indicates homology
U R.IDIHRPAQTAR.I
1668   639.3600 1276.7054 1276.7000 4.27 0 27 0.2 +1Score > 38 indicates identity
Score > 33 indicates homology
U R.IDIHRPAQTAR.I
1669   426.5761 1276.7065 1276.7000 5.07 0 17 0.32 +1Score > 38 indicates identity
Score > 25 indicates homology
U R.IDIHRPAQTAR.I
2077 +2 369.7336 1474.9053 1474.8984 4.69 0 10 1 +1Score > 26 indicates identity
Score > 23 indicates homology
U R.ITIHTARPGIVIGK.K
2078   492.6430 1474.9072 1474.8984 5.96 0 24 0.013 +1Score > 25 indicates identity
Score > 17 indicates homology
U R.ITIHTARPGIVIGK.K
3509   744.3650 2230.0732 2230.0430 13.5 1 48 0.00017 +1Score > 39 indicates identity
Score > 22 indicates homology
U R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)

1 subset or intersection (19 subset proteins in total)

Score Mass Subset of
RS3_CHRSD 24 26097 111.1
30S ribosomal protein S3 OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpsC PE=3 SV=1
18 samesets of RS3_CHRSD
RS3_PSE14 24 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsC PE=3 SV=1
RS3_PSEA7 24 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsC PE=3 SV=1
RS3_PSEAB 24 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsC PE=3 SV=1
RS3_PSEAE 24 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa GN=rpsC PE=3 SV=1
RS3_PSEF5 24 25676
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsC PE=3 SV=1
RS3_PSEPF 24 25737
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsC PE=3 SV=1
RS3_PSESM 24 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. tomato GN=rpsC PE=3 SV=1
RS3_PSEU2 24 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsC PE=3 SV=1
RS3_PSEMY 24 25525
30S ribosomal protein S3 OS=Pseudomonas mendocina (strain ymp) GN=rpsC PE=3 SV=1
RS3_HAHCH 24 25467
30S ribosomal protein S3 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsC PE=3 SV=1
RS3_MARAV 24 25498
30S ribosomal protein S3 OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=rpsC PE=3 SV=1
RS3_FRATF 24 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=rpsC PE=3 SV=1
RS3_FRATH 24 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain LVS) GN=rpsC PE=3 SV=1
RS3_FRATM 24 24848
30S ribosomal protein S3 OS=Francisella tularensis subsp. mediasiatica (strain FSC147) GN=rpsC PE=3 SV=1
RS3_FRATN 24 24792
30S ribosomal protein S3 OS=Francisella tularensis subsp. novicida (strain U112) GN=rpsC PE=3 SV=1
RS3_FRATO 24 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=rpsC PE=3 SV=1
RS3_FRATW 24 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=rpsC PE=3 SV=1
RS3_NITOC 24 24932
30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1

+112

Accession Score Description
1 THIO_PSEAE 52 Thioredoxin OS=Pseudomonas aeruginosa GN=trxA PE=3 SV=1

+113

Accession Score Description
1 RS18_PSEE4 52 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+114

Accession Score Description
1 POLG_HAVNO 51 Genome polyprotein OS=Human hepatitis A virus genotype IIIA (isolate NOR-21) PE=3 SV=4

+115

Accession Score Description
1 ASPA_PSEFL 51 Aspartate ammonia-lyase OS=Pseudomonas fluorescens GN=aspA PE=3 SV=1

+116

Accession Score Description
1 DHQS_METLZ 51 3-dehydroquinate synthase OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=Mlab_1653 PE=3 SV=1

+117

Accession Score Description
1 RL16_PSEF5 50 50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplP PE=3 SV=1

+118

Accession Score Description
1 RPOB_OLTVI 50 DNA-directed RNA polymerase subunit beta OS=Oltmannsiellopsis viridis GN=rpoB PE=3 SV=1

+119

Accession Score Description
1 RRAA_PSEPG 50 Regulator of ribonuclease activity A OS=Pseudomonas putida (strain GB-1) GN=rraA PE=3 SV=1

+120

Accession Score Description
1 CYOB_PSEPU 50 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1
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