MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita5 sp |
| MS data file | : | PRT1270_T-BRSC_5_20250714121805.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:42:02 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 321 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 11.1 | DNAK_PSEPK |
321 | 68873 | 17 (11) | 14 (9) | 0.43 | |
| Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 | |||||||
| 1 sameset of DNAK_PSEPK | |||||||
| DNAK_PSEP1 |
321 | 65566 | 17 (11) | 14 (9) | 0.46 | ||
| Chaperone protein dnaK OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=dnaK PE=2 SV=1 | |||||||
17 peptide matches (17 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 423 |
358.2220 | 714.4294 | 714.4276 | 2.61 | 0 | 35 | 0.36 | 1Score > 43 indicates identity |
R.IAGLDVK.R | ||
| 575 |
394.7499 | 787.4852 | 787.4803 | 6.22 | 0 | 12 | 0.9 | 1Score > 37 indicates identity Score > 25 indicates homology |
U | K.TQSIVIK.A | |
| 601 |
400.2392 | 798.4638 | 798.4599 | 4.89 | 0 | 21 | 0.051 | 1Score > 38 indicates identity Score > 21 indicates homology |
U | K.VSAPVAQK.M | |
| 833 |
451.7706 | 901.5266 | 901.5233 | 3.75 | 1 | 15 | 0.67 | 1Score > 39 indicates identity Score > 25 indicates homology |
K.NTTIPTKK.S | ||
| 852 |
456.7285 | 911.4424 | 911.4389 | 3.90 | 1 | 23 | 0.17 | 1Score > 40 indicates identity Score > 28 indicates homology |
U | K.EVADFFGK.E | |
| 894 |
463.7581 | 925.5016 | 925.4981 | 3.82 | 0 | 39 | 0.0065 | 1Score > 38 indicates identity Score > 30 indicates homology |
U | K.NDPLALQR.L | |
| 1485 |
399.8721 | 1196.5945 | 1196.5898 | 3.91 | 0 | 65 | 0.00021 | 1Score > 40 indicates identity |
U | R.NQGDALVHSTR.K | |
| 1486 |
599.3048 | 1196.5950 | 1196.5898 | 4.39 | 0 | 60 | 0.00072 | 1Score > 41 indicates identity |
U | R.NQGDALVHSTR.K | |
| 1515 |
606.3290 | 1210.6434 | 1210.6346 | 7.30 | 0 | 25 | 0.19 | 1Score > 40 indicates identity Score > 30 indicates homology |
K.FDLADIPPAPR.G | ||
| 1684 |
643.3636 | 1284.7126 | 1284.7038 | 6.90 | 0 | 59 | 0.00061 | 1Score > 39 indicates identity |
U | K.IDDVILVGGQTR.M | |
| 1718 |
651.8101 | 1301.6056 | 1301.6000 | 4.33 | 1 | 57 | 0.0001 | 1Score > 40 indicates identity Score > 30 indicates homology |
U | K.ANNGDAWVEAAGK.E | |
| 2206 |
513.9500 | 1538.8282 | 1538.8205 | 4.97 | 0 | 22 | 0.015 | 1Score > 38 indicates identity Score > 16 indicates homology |
U | R.QAVTNPHNTLFAVK.R | |
| 2539 |
839.4335 | 1676.8524 | 1676.8443 | 4.85 | 0 | 94 | 2.9e-007 | 1Score > 41 indicates identity |
U | R.IINEPTAAALAYGMDK.A | |
| 3300 |
712.0498 | 2133.1276 | 2133.0954 | 15.1 | 1 | 25 | 0.01 | 1Score > 37 indicates identity Score > 18 indicates homology |
U | R.TTPSIVAYANDGEILVGQSAK.R | |
| 3301 |
1067.5743 | 2133.1340 | 2133.0954 | 18.1 | 1 | 19 | 0.098 | 1Score > 37 indicates identity Score > 21 indicates homology |
U | R.TTPSIVAYANDGEILVGQSAK.R | |
| 3308 |
1068.0664 | 2134.1182 | 2134.0794 | 18.2 | 1 | 73 | 2.8e-007 | 1Score > 38 indicates identity Score > 20 indicates homology |
U | R.TTPSIVAYANDGEILVGQSAK.R + Deamidated (NQ) | |
| 3887 |
835.0869 | 2502.2389 | 2502.1987 | 16.1 | 1 | 110 | 3.3e-009 | 1Score > 38 indicates identity |
U | K.SQVFSTADDNQSAVTIHVLQGER.K + Deamidated (NQ) |
13 subsets and intersections (92 subset proteins in total)12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 296 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 288 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 268 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 266 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 256 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 254 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 253 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 226 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSEAE | 211 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
| 2 | PORF_PSESY | 177 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
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