MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 101–110 (out of 240)

Page: Previous 1 …6 7 8 9 10 11 12 13 14 15 16 … 24 Next 

+101

Accession Score Description
1 FABG_PSEAE 58 3-oxoacyl-[acyl-carrier-protein] reductase OS=Pseudomonas aeruginosa GN=fabG PE=3 SV=1

+102

Accession Score Description
1 RL19_PSEE4 57 50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1

+103

Accession Score Description
1 RPOZ_ALCBS 57 DNA-directed RNA polymerase subunit omega OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpoZ PE=3 SV=1

+104

Accession Score Description
1 ILVD_PSEFS 57 Dihydroxy-acid dehydratase OS=Pseudomonas fluorescens (strain SBW25) GN=ilvD PE=3 SV=1

+105

Accession Score Description
1 RS7_PSEE4 56 30S ribosomal protein S7 OS=Pseudomonas entomophila (strain L48) GN=rpsG PE=3 SV=1

+106

Accession Score Description
1 SYD_PSEPG 56 Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1

+107

Accession Score Description
1 RS20_PSE14 54 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

-108

Accession Score Description
1 RS1_PSEAE 53 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
Score Mass Matches Sequences emPAI
108.1 RS1_PSEAE 53 61946 11 (4) 6 (1) 0.08
30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
7 samesets of RS1_PSEAE
RS1_BUCAI 53 62849 10 (4) 5 (1) 0.07
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=rpsA PE=3 SV=1
RS1_BUCAP 53 62605 9 (4) 4 (1) 0.07
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Schizaphis graminum GN=rpsA PE=3 SV=1
RS1_ECO57 53 61235 9 (4) 4 (1) 0.08
30S ribosomal protein S1 OS=Escherichia coli O157:H7 GN=rpsA PE=3 SV=1
RS1_ECOL6 53 61235 9 (4) 4 (1) 0.08
30S ribosomal protein S1 OS=Escherichia coli O6 GN=rpsA PE=3 SV=1
RS1_ECOLI 53 61235 9 (4) 4 (1) 0.08
30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1
RS1_PROSP 53 41702 9 (4) 4 (1) 0.11
30S ribosomal protein S1 (Fragment) OS=Providencia sp. GN=rpsA PE=3 SV=1
RS1_SHIFL 53 61235 9 (4) 4 (1) 0.08
30S ribosomal protein S1 OS=Shigella flexneri GN=rpsA PE=3 SV=1

-11 peptide matches (8 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
184   587.3540 586.3467 586.3439 4.89 0 21 0.11 +1Score > 44 indicates identity
Score > 24 indicates homology		 U K.GVINGK.V
229   308.7059 615.3972 615.3956 2.75 0 27 0.15 +1Score > 42 indicates identity
Score > 31 indicates homology		 R.VSLGLK.Q
254   630.4202 629.4129 629.4112 2.75 0 14 0.76 +3Score > 38 indicates identity
Score > 25 indicates homology		 R.ISLGIK.Q
256 +1 315.7140 629.4134 629.4112 3.58 0 29 0.31 +1Score > 38 indicates identity
Score > 36 indicates homology		 R.ISLGIK.Q
569   394.2267 786.4388 786.4348 5.15 0 39 0.2 +1Score > 44 indicates identity R.NNVVVSR.R
1345   568.2993 1134.5840 1134.5670 15.1 0 19 0.26 +1Score > 42 indicates identity
Score > 26 indicates homology		 U K.GGFTVDVNGIR.A + Deamidated (NQ)
2179   763.4537 1524.8928 1524.8777 9.96 0 48 0.00049 +1Score > 33 indicates identity
Score > 27 indicates homology		 U R.AFLPGSLVDVRPVR.D
2181 +2 509.3055 1524.8947 1524.8777 11.2 0 25 0.022 +1Score > 33 indicates identity
Score > 21 indicates homology		 U R.AFLPGSLVDVRPVR.D

+109

Accession Score Description
1 ETFB_PSEAE 53 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+110

Accession Score Description
1 LEU1_PSEP1 52 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1
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