Rita5 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita5 sp
MS data file	:	PRT1270_T-BRSC_5_20250714121805.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:42:02 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita5 sp

MS data file

PRT1270_T-BRSC_5_20250714121805.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:42:02 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,997

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,997

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (

) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Protein families 101–110 (out of 240)

Previous 1 …6 7 8 9 10 11 12 13 14 15 16 … 24 Next

101

		Accession	Score	Description
	1	FABG_PSEAE	58	3-oxoacyl-[acyl-carrier-protein] reductase OS=Pseudomonas aeruginosa GN=fabG PE=3 SV=1

102

		Accession	Score	Description
	1	RL19_PSEE4	57	50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1

103

		Accession	Score	Description
	1	RPOZ_ALCBS	57	DNA-directed RNA polymerase subunit omega OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpoZ PE=3 SV=1

104

		Accession	Score	Description
	1	ILVD_PSEFS	57	Dihydroxy-acid dehydratase OS=Pseudomonas fluorescens (strain SBW25) GN=ilvD PE=3 SV=1

105

		Accession	Score	Description
	1	RS7_PSEE4	56	30S ribosomal protein S7 OS=Pseudomonas entomophila (strain L48) GN=rpsG PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
105.1	RS7_PSEE4	56	17567	4 (3)	4 (3)	0.46
	30S ribosomal protein S7 OS=Pseudomonas entomophila (strain L48) GN=rpsG PE=3 SV=1
	6 samesets of RS7_PSEE4
	RS7_PSEMY	56	17567	4 (3)	4 (3)	0.46
	30S ribosomal protein S7 OS=Pseudomonas mendocina (strain ymp) GN=rpsG PE=3 SV=1
	RS7_PSEP1	56	17567	4 (3)	4 (3)	0.46
	30S ribosomal protein S7 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsG PE=3 SV=1
	RS7_PSEPG	56	17567	4 (3)	4 (3)	0.46
	30S ribosomal protein S7 OS=Pseudomonas putida (strain GB-1) GN=rpsG PE=3 SV=1
	RS7_PSEPK	56	17567	4 (3)	4 (3)	0.46
	30S ribosomal protein S7 OS=Pseudomonas putida (strain KT2440) GN=rpsG PE=3 SV=1
	RS7_PSEPW	56	17567	4 (3)	4 (3)	0.46
	30S ribosomal protein S7 OS=Pseudomonas putida (strain W619) GN=rpsG PE=3 SV=1
	RS7_AZOVD	56	17523	3 (3)	3 (3)	0.46
	30S ribosomal protein S7 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsG PE=3 SV=1

4 peptide matches (4 non-duplicate, 0 duplicate)

Query	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
884	461.7452	921.4758	921.4708	5.43	0	26	0.078	1Score > 41 indicates identity Score > 27 indicates homology	U	R.WLVDYAR.K
1229	539.8141	1077.6136	1077.6070	6.16	0	35	0.0063	1Score > 37 indicates identity Score > 26 indicates homology	U	R.IVYGALDTVK.A
1595	619.8798	1237.7450	1237.7282	13.6	1	35	0.03	1Score > 32 indicates identity	U	K.ALDAIAPLVEVK.S
2598	572.3154	1713.9244	1713.9162	4.75	1	39	0.0013	1Score > 38 indicates identity Score > 23 indicates homology	U	R.VGGATYQVPVEVRPSR.R

2 subsets and intersections (18 subset proteins in total)

	Score	Mass	Subset of
RS7_PSE14	47	17636	105.1
30S ribosomal protein S7 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsG PE=3 SV=1
10 samesets of RS7_PSE14
RS7_PSEA7	47	17493
30S ribosomal protein S7 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsG PE=3 SV=1
RS7_PSEA8	47	17493
30S ribosomal protein S7 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsG PE=3 SV=1
RS7_PSEAB	47	17493
30S ribosomal protein S7 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsG PE=3 SV=1
RS7_PSEAE	47	17493
30S ribosomal protein S7 OS=Pseudomonas aeruginosa GN=rpsG PE=3 SV=1
RS7_PSEF5	47	17650
30S ribosomal protein S7 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsG PE=3 SV=1
RS7_PSEFS	47	17636
30S ribosomal protein S7 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsG PE=3 SV=1
RS7_PSEPF	47	17566
30S ribosomal protein S7 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsG PE=3 SV=1
RS7_PSESM	47	17654
30S ribosomal protein S7 OS=Pseudomonas syringae pv. tomato GN=rpsG PE=3 SV=1
RS7_PSEU2	47	17640
30S ribosomal protein S7 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsG PE=3 SV=1
RS7_PSEU5	47	17577
30S ribosomal protein S7 OS=Pseudomonas stutzeri (strain A1501) GN=rpsG PE=3 SV=1
RS7_ALCBS	39	17552	105.1
30S ribosomal protein S7 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsG PE=3 SV=1
6 samesets of RS7_ALCBS
RS7_CHRSD	39	17667
30S ribosomal protein S7 OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpsG PE=3 SV=1
RS7_HAHCH	39	17698
30S ribosomal protein S7 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsG PE=3 SV=1
RS7_MARAV	39	17698
30S ribosomal protein S7 OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=rpsG PE=3 SV=1
RS7_PARDP	39	17925
30S ribosomal protein S7 OS=Paracoccus denitrificans (strain Pd 1222) GN=rpsG PE=3 SV=1
RS7_RHOCA	39	17891
30S ribosomal protein S7 OS=Rhodobacter capsulatus GN=rpsG PE=3 SV=1
RS7_TERTT	39	17745
30S ribosomal protein S7 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rpsG PE=3 SV=1

106

		Accession	Score	Description
	1	SYD_PSEPG	56	Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=aspS PE=3 SV=1

107

		Accession	Score	Description
	1	RS20_PSE14	54	30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

108

		Accession	Score	Description
	1	RS1_PSEAE	53	30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1

109

		Accession	Score	Description
	1	ETFB_PSEAE	53	Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

110

		Accession	Score	Description
	1	LEU1_PSEP1	52	2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

Previous 1 …6 7 8 9 10 11 12 13 14 15 16 … 24 Next

Not what you expected? Try the select summary.

MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 101–110 (out of 240)

101

102

103

104

105

4 peptide matches (4 non-duplicate, 0 duplicate)

2 subsets and intersections (18 subset proteins in total)

106

107

108

109

110