MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 240)

Page: Previous 1 …5 6 7 8 9 10 11 12 13 14 15 … 24 Next 

+91

Accession Score Description
1 CARA_PSEPK 65 Carbamoyl-phosphate synthase small chain OS=Pseudomonas putida (strain KT2440) GN=carA PE=3 SV=1

+92

Accession Score Description
1 BFR_SALTY 64 Bacterioferritin OS=Salmonella typhimurium GN=bfr PE=3 SV=1

+93

Accession Score Description
1 RL14_PSE14 62 50S ribosomal protein L14 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplN PE=3 SV=1

+94

Accession Score Description
1 SYS_ALKOO 62 Seryl-tRNA synthetase OS=Alkaliphilus oremlandii (strain OhILAs) GN=serS PE=3 SV=1

+95

Accession Score Description
1 OPRI_PSEAE 61 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+96

Accession Score Description
1 RL13_PSEP1 60 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1

+97

Accession Score Description
1 G6PI1_PSEPK 60 Glucose-6-phosphate isomerase 1 OS=Pseudomonas putida (strain KT2440) GN=pgi1 PE=3 SV=1

+98

Accession Score Description
1 RS4_PSEP1 59 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1

+99

Accession Score Description
1 CYOA_PSEPU 59 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

-100

Accession Score Description
1 ENO_PSEPK 59 Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1
Score Mass Matches Sequences emPAI
100.1 ENO_PSEPK 59 45633 3 (2) 3 (2) 0.10
Enolase OS=Pseudomonas putida (strain KT2440) GN=eno PE=3 SV=1
2 samesets of ENO_PSEPK
ENO_PSEPW 59 45637 3 (2) 3 (2) 0.10
Enolase OS=Pseudomonas putida (strain W619) GN=eno PE=3 SV=1
ENO_PSEE4 59 45602 3 (2) 3 (2) 0.10
Enolase OS=Pseudomonas entomophila (strain L48) GN=eno PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1844   681.8202 1361.6258 1361.6139 8.75 1 33 0.0021 +1Score > 40 indicates identity
Score > 18 indicates homology		 U K.SFDAEGFADYLK.G
2227   774.9256 1547.8366 1547.8195 11.0 0 52 0.0011 +1Score > 39 indicates identity
Score > 35 indicates homology		 U K.VQLVGDDLFVTNTK.I
2975   967.4468 1932.8790 1932.8701 4.63 1 5 2 +6Score > 39 indicates identity
Score > 21 indicates homology		 U R.GLNTAVGDEGGFAPNLASNE.D + Deamidated (NQ)

1 subset or intersection (59 subset proteins in total)

Score Mass Subset of
ENO1_METHJ 52 46300 100.1
Enolase 1 OS=Methanospirillum hungatei (strain JF-1 / DSM 864) GN=eno1 PE=3 SV=1
+58 samesets of ENO1_METHJ
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