MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 240)

Page: 1 2 3 4 5 6 … 24 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1313 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 105 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1215 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 945 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEU5 708 ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1
4 ATPB_LEGPA 195 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
3 ATPB_PSE14 666 ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 849 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH602_SORC5 95 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 777 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 168 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 155 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+5

Accession Score Description
1 ARCA_PSEPK 648 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 454 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 ADHP_ECOLI 447 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+8

Accession Score Description
1 OTCC_PSEPK 407 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+9

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 398 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
6 RPOB_CHRSD 115 DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
3 RPOB_SACD2 141 DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 123 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEE4 267 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
7 RPOC_COXBN 77 DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 131 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

-10

Accession Score Description
1 SAHH_PSEP1 340 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
Score Mass Matches Sequences emPAI
10.1 SAHH_PSEP1 340 51870 13 (9) 12 (9) 0.48
Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

-13 peptide matches (13 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
617   402.2412 802.4678 802.4622 7.00 0 30 0.13 +1Score > 39 indicates identity
Score > 33 indicates homology		 U R.LLDMLAK.G
814   449.2452 896.4758 896.4716 4.79 0 21 0.28 +1Score > 38 indicates identity
Score > 28 indicates homology		 U R.HSLNDAIK.R
821   449.7870 897.5594 897.5535 6.61 1 16 0.68 +1Score > 36 indicates identity
Score > 27 indicates homology		 U R.LTVEVLPK.K
895   309.8359 926.4859 926.4821 4.02 0 14 0.9 +1Score > 39 indicates identity
Score > 26 indicates homology		 U R.GTDHLLSGK.Q
896   464.2504 926.4862 926.4821 4.43 0 30 0.025 +1Score > 39 indicates identity
Score > 26 indicates homology		 U R.GTDHLLSGK.Q
1281   553.8180 1105.6214 1105.6132 7.47 0 39 0.0015 +1Score > 38 indicates identity
Score > 23 indicates homology		 U R.GFGGVVTQLTK.Q
1398   579.8226 1157.6306 1157.6193 9.81 0 83 1.6e-006 +1Score > 41 indicates identity
Score > 37 indicates homology		 U K.VADISLAAWGR.R
1541   610.3413 1218.6680 1218.6608 5.91 0 55 0.00017 +1Score > 40 indicates identity
Score > 30 indicates homology		 U K.QALVIGYGDVGK.G
1634   628.8488 1255.6830 1255.6772 4.64 0 47 0.00042 +1Score > 40 indicates identity
Score > 26 indicates homology		 U K.VPAINVNDSVTK.S
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_5_20250714121805.raw

Score > 40 indicates identity

Score > 31 indicates homology
1791   445.9097 1334.7073 1334.7055 1.34 0 59 7.4e-005 -1Score > 40 indicates identity
Score > 31 indicates homology		 U R.LVNLGNATGHPSR.I
-0.67 0 14 2.3 2 LATLPGTSYGNLK   + Deamidated (NQ)
-7.07 1 14 2.4 3 LRGLGNHQVNAR   + Deamidated (NQ)
-0.65 1 12 3.7 4 VLNLLTNKQYE   + Deamidated (NQ)
-0.65 1 12 3.7 4 VLNLLTNKQYE   + Deamidated (NQ)
-1.67 0 10 7.2 6 ALPTLSAHWPSR  
6.75 0 9 8.8 7 YYLNHNTALVK  
18.2 1 9 9 8 IVNLNYLESNR   + Deamidated (NQ)
-7.07 1 8 11 9 LRGLGNHQVNAR   + Deamidated (NQ)
-7.07 1 7 12 10 LRGLGNHQVNAR   + Deamidated (NQ)
3560   1127.0585 2252.1024 2252.0597 19.0 1 99 5.9e-008 +1Score > 39 indicates identity U R.TGAGSFDPQNDDYLILLAEGR.L + Deamidated (NQ)
3623   1150.6058 2299.1970 2299.1558 17.9 1 70 8e-007 +1Score > 38 indicates identity
Score > 22 indicates homology		 U R.IMDGSFANQVLAQIFLFEQK.F + Deamidated (NQ)
3940   851.7465 2552.2177 2552.1853 12.7 1 83 2.6e-008 +1Score > 38 indicates identity
Score > 20 indicates homology		 U K.DGQPWDANMILDDGGDLTELLHK.K

+17 subsets and intersections (29 subset proteins in total)

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