MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 240)


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+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1313 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 105 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1215 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 945 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEU5 708 ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1
4 ATPB_LEGPA 195 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
3 ATPB_PSE14 666 ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 849 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH602_SORC5 95 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 777 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 168 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 155 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-5

Accession Score Description
1 ARCA_PSEPK 648 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1
Score Mass Matches Sequences emPAI
5.1 ARCA_PSEPK 648 46775 31 (22) 13 (10) 0.96
Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-31 peptide matches (20 non-duplicate, 11 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
234   621.2911 620.2838 620.2806 5.23 0 13 0.13 +1Score > 42 indicates identity
Score > 17 indicates homology
U R.DPINY.-
389   702.3952 701.3879 701.3860 2.68 0 25 0.76 +1Score > 42 indicates identity
Score > 37 indicates homology
K.WILDR.K
391   351.7019 701.3892 701.3860 4.56 0 19 0.67 +1Score > 42 indicates identity
Score > 30 indicates homology
K.WILDR.K
891   925.5732 924.5659 924.5644 1.64 1 46 0.0034 +1Score > 34 indicates identity U K.EVIVAGLPK.S
892 -1 463.2916 924.5686 924.5644 4.59 1 28 0.26 +1Score > 34 indicates identity U K.EVIVAGLPK.S
893   463.2923 924.5700 924.5644 6.10 1 (21) 0.16 +1Score > 34 indicates identity
Score > 25 indicates homology
U K.EVIVAGLPK.S
944   316.4931 946.4575 946.4508 7.01 1 17 0.36 +1Score > 42 indicates identity
Score > 25 indicates homology
U K.YGVHSEAGK.L
1089   504.7437 1007.4728 1007.4713 1.57 0 27 0.31 +1Score > 42 indicates identity
Score > 35 indicates homology
U R.DHFDFVTK.M
1090   336.8321 1007.4745 1007.4713 3.18 0 31 0.048 +1Score > 42 indicates identity
Score > 30 indicates homology
U R.DHFDFVTK.M
1246   1086.5675 1085.5602 1085.5505 8.92 1 47 0.014 +1Score > 41 indicates identity U R.SWLEGLEPR.H
1247 +2 543.7879 1085.5612 1085.5505 9.87 1 45 0.023 +1Score > 41 indicates identity U R.SWLEGLEPR.H
1262   1095.5824 1094.5751 1094.5720 2.85 0 59 0.00052 +1Score > 39 indicates identity U R.NTYTNTLLR.K
1264 +1 548.2959 1094.5772 1094.5720 4.79 0 59 0.00063 +1Score > 39 indicates identity U R.NTYTNTLLR.K
1616   623.3654 1244.7162 1244.7017 11.7 1 26 0.015 +1Score > 36 indicates identity
Score > 20 indicates homology
U R.DLVTVFPEVVK.E
1675   640.8647 1279.7148 1279.7023 9.77 1 58 0.00017 +1Score > 37 indicates identity
Score > 33 indicates homology
U R.QETLLTTAIYK.F
1927 +2 701.4050 1400.7954 1400.7776 12.8 0 92 1.7e-007 +1Score > 37 indicates identity U R.QAIGQLAQNLFAK.G
2279 +2 785.9321 1569.8496 1569.8363 8.53 1 79 3.9e-006 +1Score > 39 indicates identity
Score > 37 indicates homology
U K.ITPDTVGVGLTNEVR.S
2280   524.2906 1569.8500 1569.8363 8.74 1 51 4.7e-005 +1Score > 39 indicates identity
Score > 21 indicates homology
U K.ITPDTVGVGLTNEVR.S
3128 +1 1019.5478 2037.0810 2037.0565 12.1 1 100 1.7e-008 +1Score > 37 indicates identity
Score > 35 indicates homology
U R.GVDVLEMHNLLTDIVQNK.E
3130 +1 680.0353 2037.0841 2037.0565 13.5 1 60 1.7e-005 +1Score > 37 indicates identity
Score > 25 indicates homology
U R.GVDVLEMHNLLTDIVQNK.E
3830 +1 816.0706 2445.1900 2445.1448 18.5 1 62 0.00024 +1Score > 39 indicates identity U R.EQWDDGNNVVAIEPGVVIGYDR.N + Deamidated (NQ)

4 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
ARCA_PSEPU 169 46933 5.1
Arginine deiminase OS=Pseudomonas putida GN=arcA PE=1 SV=1
ARCA_PSEAE 111 46806 5.1
Arginine deiminase OS=Pseudomonas aeruginosa GN=arcA PE=1 SV=2
ARCA1_RHIME 105 46140 5.1
Arginine deiminase 1 OS=Rhizobium meliloti GN=arcA1 PE=3 SV=1
2 samesets of ARCA1_RHIME
ARCA_RHIET 105 46140
Arginine deiminase OS=Rhizobium etli GN=arcA PE=3 SV=1
ARCA_RHIE6 105 46196
Arginine deiminase OS=Rhizobium etli (strain CIAT 652) GN=arcA PE=3 SV=1
ARCA2_RHIME 85 46880 5.1
Arginine deiminase 2 OS=Rhizobium meliloti GN=arcA2 PE=3 SV=1
6 samesets of ARCA2_RHIME
ARCA_BURMA 85 46422
Arginine deiminase OS=Burkholderia mallei GN=arcA PE=3 SV=1
ARCA_BURPS 85 46422
Arginine deiminase OS=Burkholderia pseudomallei GN=arcA PE=3 SV=1
ARCA_PSEMY 85 46334
Arginine deiminase OS=Pseudomonas mendocina (strain ymp) GN=arcA PE=3 SV=1
ARCA_BRAJA 85 46780
Arginine deiminase OS=Bradyrhizobium japonicum GN=arcA PE=3 SV=1
ARCA_MARMS 85 46613
Arginine deiminase OS=Marinomonas sp. (strain MWYL1) GN=arcA PE=3 SV=1
ARCA_RHILO 85 46031
Arginine deiminase OS=Rhizobium loti GN=arcA PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 454 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 ADHP_ECOLI 447 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+8

Accession Score Description
1 OTCC_PSEPK 407 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+9

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 398 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
6 RPOB_CHRSD 115 DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
3 RPOB_SACD2 141 DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 123 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEE4 267 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
7 RPOC_COXBN 77 DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 131 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+10

Accession Score Description
1 SAHH_PSEP1 340 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
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