MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita5 sp
MS data file : PRT1270_T-BRSC_5_20250714121805.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:42:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,997

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 240)

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-1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1313 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 105 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1215 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
EFTU2_PSEPK 1313 43793 81 (57) 19 (16) 4.67
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
1 sameset of EFTU2_PSEPK
EFTU_PSEE4 1313 43793 81 (57) 19 (16) 4.67
Elongation factor Tu OS=Pseudomonas entomophila (strain L48) GN=tuf1 PE=3 SV=1
EFTU1_PSEPK 1215 43810 80 (56) 19 (16) 4.39
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 samesets of EFTU1_PSEPK
EFTU_PSEPG 1215 43810 80 (56) 19 (16) 4.39
Elongation factor Tu OS=Pseudomonas putida (strain GB-1) GN=tuf1 PE=3 SV=1
EFTU_PSEPW 1215 43810 80 (56) 19 (16) 4.39
Elongation factor Tu OS=Pseudomonas putida (strain W619) GN=tuf1 PE=3 SV=1
EFTU_PSEP1 1215 43810 80 (56) 19 (16) 4.39
Elongation factor Tu OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tuf PE=3 SV=1
EFTU_CARRP 105 44439 10 (5) 2 (2) 0.16
Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

-94 peptide matches (50 non-duplicate, 44 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
363 +1 345.2009 688.3872 688.3868 0.64 0 31 0.12 +1Score > 43 indicates identity
Score > 35 indicates homology		 X X R.GTVVTGR.I
366 +1 689.3967 688.3894 688.3868 3.80 0 33 0.086 +1Score > 41 indicates identity
Score > 35 indicates homology		 X X R.GTVVTGR.I
385   702.3800 701.3727 701.3708 2.76 1 35 0.22 +1Score > 45 indicates identity
Score > 41 indicates homology		 X X K.LLDEGR.A
388 -1 351.6940 701.3734 701.3708 3.79 1 22 0.35 +1Score > 45 indicates identity
Score > 30 indicates homology		 X X K.LLDEGR.A
386   351.6939 701.3732 701.3708 3.51 1 (17) 0.59 +1Score > 45 indicates identity
Score > 27 indicates homology		 X X K.LLDEGR.A
548 +3 388.7096 775.4046 775.4017 3.78 0 21 0.077 +1Score > 42 indicates identity
Score > 22 indicates homology		 U X X X R.HTPFFK.G
549   776.4125 775.4052 775.4017 4.52 0 33 0.027 +1Score > 42 indicates identity
Score > 30 indicates homology		 U X X X R.HTPFFK.G
607 +1 801.4872 800.4799 800.4756 5.38 0 51 0.0065 +1Score > 42 indicates identity U X X R.TVGAGVVAK.I
608 +1 401.2473 800.4800 800.4756 5.54 0 37 0.011 +1Score > 42 indicates identity
Score > 30 indicates homology		 U X X R.TVGAGVVAK.I
745   867.5076 866.5003 866.4974 3.40 1 51 0.0019 +1Score > 37 indicates identity U X X R.EHILLSR.Q
746 +2 434.2581 866.5016 866.4974 4.93 1 40 0.022 +1Score > 36 indicates identity U X X R.EHILLSR.Q
948 +1 947.5551 946.5478 946.5447 3.26 0 58 0.0005 +1Score > 38 indicates identity U X X K.TTLTAALTR.V
949 +1 474.2812 946.5478 946.5447 3.28 0 81 9.1e-007 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X X K.TTLTAALTR.V
1084   1005.5056 1004.4983 1004.4961 2.25 1 60 0.00027 +1Score > 42 indicates identity
Score > 37 indicates homology		 U X X K.TIAMEDGLR.F
1086 +1 503.2573 1004.5000 1004.4961 3.97 1 38 0.045 +1Score > 42 indicates identity
Score > 37 indicates homology		 U X X K.TIAMEDGLR.F
1255   544.7826 1087.5506 1087.5444 5.73 1 79 1.2e-005 +1Score > 42 indicates identity U X X R.AGENCGVLLR.G
1390   1156.6329 1155.6256 1155.6176 6.97 1 56 0.0012 +1Score > 39 indicates identity U X X K.FTAEVYVLSK.E
1391 +4 578.8205 1155.6264 1155.6176 7.68 1 46 0.00059 +1Score > 39 indicates identity
Score > 26 indicates homology		 U X X K.FTAEVYVLSK.E
1576   617.3139 1232.6132 1232.6091 3.39 0 23 0.11 +1Score > 42 indicates identity
Score > 26 indicates homology		 U X X K.GYRPQFYFR.T
1578 +2 411.8791 1232.6155 1232.6091 5.19 0 30 0.026 +1Score > 42 indicates identity
Score > 27 indicates homology		 U X X K.GYRPQFYFR.T
1589   413.5783 1237.7131 1237.7030 8.11 1 61 0.00015 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
1591 +3 619.8662 1237.7178 1237.7030 12.0 1 69 2.5e-005 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
2070 +3 738.4449 1474.8752 1474.8548 13.9 0 75 2.4e-006 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2072 +2 492.6325 1474.8757 1474.8548 14.2 0 43 0.0032 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2288 +1 315.7944 1573.9356 1573.9304 3.30 0 10 0.44 +1Score > 30 indicates identity
Score > 19 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2290   394.4916 1573.9373 1573.9304 4.37 0 33 0.00075 +1Score > 29 indicates identity
Score > 15 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2291   525.6531 1573.9375 1573.9304 4.48 0 43 0.0024 +1Score > 29 indicates identity
Score > 29 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2294   788.4753 1574.9360 1574.9144 13.7 0 40 0.0055 +1Score > 29 indicates identity U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2295   525.9877 1574.9413 1574.9144 17.0 0 25 0.14 +1Score > 29 indicates identity U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2395   538.9683 1613.8831 1613.8665 10.3 1 53 0.0003 +1Score > 38 indicates identity
Score > 30 indicates homology		 U X K.LVETLDAYIPEPVR.A
2397 +3 807.9506 1613.8866 1613.8665 12.5 1 88 1.1e-007 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X K.LVETLDAYIPEPVR.A
2429 +2 815.9462 1629.8778 1629.8614 10.1 1 92 1.9e-007 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X K.LVETLDSYIPEPVR.A
2680   883.9712 1765.9278 1765.9224 3.09 0 82 2.6e-006 +1Score > 39 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2681   442.4900 1765.9309 1765.9224 4.81 0 44 0.0024 +1Score > 38 indicates identity
Score > 30 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T
2683   884.4731 1766.9316 1766.9064 14.3 0 72 1.1e-006 +1Score > 39 indicates identity
Score > 25 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2686 +2 354.3938 1766.9326 1766.9064 14.8 0 28 0.16 +1Score > 39 indicates identity
Score > 32 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2688 +1 442.7407 1766.9337 1766.9064 15.4 0 35 0.0014 +1Score > 39 indicates identity
Score > 19 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2689 +2 589.9852 1766.9338 1766.9064 15.5 0 44 0.00019 +1Score > 39 indicates identity
Score > 19 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2695   442.9534 1767.7845 1767.7787 3.25 0 17 0.055 +1Score > 39 indicates identity
Score > 17 indicates homology		 U X X R.HYAHVDCPGHADYVK.N
2728 +1 596.9835 1787.9287 1787.9166 6.75 1 59 6e-005 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2729 +1 894.9775 1787.9404 1787.9166 13.3 1 76 8.3e-007 +1Score > 39 indicates identity
Score > 27 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2734   597.3156 1788.9250 1788.9006 13.6 1 33 0.0037 +1Score > 40 indicates identity
Score > 21 indicates homology		 U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2748   601.3140 1800.9202 1800.9118 4.63 1 63 0.00027 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2749 +1 901.4689 1800.9232 1800.9118 6.34 1 99 7.3e-008 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2756   601.6486 1801.9240 1801.8958 15.6 1 69 6.9e-005 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
3307   712.3763 2134.1071 2134.0769 14.2 1 70 4.2e-005 +1Score > 38 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G
3311   1068.5554 2135.0962 2135.0609 16.6 1 50 2.4e-005 +1Score > 39 indicates identity
Score > 16 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ)
3347   1076.5559 2151.0972 2151.0558 19.3 1 70 3.9e-007 +1Score > 39 indicates identity
Score > 19 indicates homology		 U X X R.AIDQPFLMPIEDVFSISGR.G + Deamidated (NQ); Oxidation (M)
3441 +1 1097.0712 2192.1278 2192.1551 -12.4 1 39 0.00081 +1Score > 38 indicates identity
Score > 21 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3442 +2 731.7175 2192.1307 2192.1551 -11.2 1 47 0.00071 +1Score > 38 indicates identity
Score > 28 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3576   756.0577 2265.1513 2265.1165 15.4 0 3 1.9 +6Score > 39 indicates identity
Score > 18 indicates homology		 U X X R.DLLSTYDFPGDDTPIIIGSAR.M

+53 subsets and intersections (649 subset proteins in total)

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 945 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSEU5 708 ATP synthase subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=atpD PE=3 SV=1
4 ATPB_LEGPA 195 ATP synthase subunit beta OS=Legionella pneumophila (strain Paris) GN=atpD PE=3 SV=1
3 ATPB_PSE14 666 ATP synthase subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 849 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
2 CH602_SORC5 95 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 777 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 168 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 155 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+5

Accession Score Description
1 ARCA_PSEPK 648 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+6

Accession Score Description
1 RL1_PSEE4 454 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 ADHP_ECOLI 447 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+8

Accession Score Description
1 OTCC_PSEPK 407 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

+9

Accession Score Description
Family member distances as a dendrogram 1 RPOB_PSEPG 398 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1
6 RPOB_CHRSD 115 DNA-directed RNA polymerase subunit beta OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoB PE=3 SV=1
3 RPOB_SACD2 141 DNA-directed RNA polymerase subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoB PE=3 SV=1
5 FTSZ_PSEPK 123 Cell division protein ftsZ OS=Pseudomonas putida (strain KT2440) GN=ftsZ PE=3 SV=3
2 RPOC_PSEE4 267 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1
7 RPOC_COXBN 77 DNA-directed RNA polymerase subunit beta' OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rpoC PE=3 SV=1
4 RPOC_SYNAS 131 DNA-directed RNA polymerase subunit beta' OS=Syntrophus aciditrophicus (strain SB) GN=rpoC PE=3 SV=1

+10

Accession Score Description
1 SAHH_PSEP1 340 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
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