MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
585 366.2196 730.4246 730.4337 -12.4 0 15 0.85 +1Score > 43 indicates identity
Score > 27 indicates homology		 AISTAIR
3307 525.2578 2097.0021 2096.9911 5.24 1 15 0.2 +1Score > 39 indicates identity
Score > 20 indicates homology		 VIPGFMIQGGGFTEQMQQK + 2 Deamidated (NQ)
2764 578.6562 1732.9468 1732.9216 14.5 1 15 0.47 +1Score > 37 indicates identity
Score > 24 indicates homology		 VLDLGCGAGVIGSMIKK + Oxidation (M)
1098 485.2740 968.5334 968.5291 4.52 1 15 0.68 +1Score > 38 indicates identity
Score > 26 indicates homology		 VIPQLRNE + Deamidated (NQ)
3357 1064.5635 2127.1124 2127.0749 17.7 1 15 0.61 +1Score > 38 indicates identity
Score > 25 indicates homology		 IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
2044 695.8895 1389.7644 1389.7616 2.06 1 15 0.48 +1Score > 38 indicates identity
Score > 24 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
1377 366.8924 1097.6554 1097.6444 9.96 0 15 0.83 +1Score > 36 indicates identity
Score > 27 indicates homology		 VVQNVINALK + Deamidated (NQ)
2514 806.9548 1611.8950 1611.8944 0.39 0 15 0.24 +1Score > 37 indicates identity
Score > 21 indicates homology		 DGGISGTVIAIAGVLNR
1134 493.2785 984.5424 984.5352 7.33 0 15 0.32 +1Score > 41 indicates identity
Score > 22 indicates homology		 AVADLQNVR
1992 684.3519 1366.6892 1366.6915 -1.64 0 15 0.63 +1Score > 40 indicates identity
Score > 25 indicates homology		 GTYVCIATNALGK
2775 871.9968 1741.9790 1741.9826 -2.02 1 15 0.59 +1Score > 35 indicates identity
Score > 25 indicates homology		 KLVLDNDVDTAVSLLK
704 398.1991 794.3836 794.3810 3.32 1 15 0.57 +1Score > 41 indicates identity
Score > 25 indicates homology		 AVFENSK + Deamidated (NQ)
1269 521.7810 1041.5474 1041.5342 12.7 0 15 0.84 +1Score > 40 indicates identity
Score > 27 indicates homology		 SVLSIPNPSE
1344 541.8053 1081.5960 1081.6066 -9.78 1 15 0.6 +1Score > 38 indicates identity
Score > 25 indicates homology		 APGVGPKVAMR
1099 485.7111 969.4076 969.4113 -3.81 0 15 0.72 +1Score > 38 indicates identity
Score > 26 indicates homology		 TMFDTNPK + Deamidated (NQ); Oxidation (M)
1879 655.3304 1308.6462 1308.6422 3.10 1 15 0.35 +1Score > 41 indicates identity
Score > 23 indicates homology		 KSANNAHQVPDK + Deamidated (NQ)
1995 684.3529 1366.6912 1366.6803 8.05 1 15 0.8 +1Score > 40 indicates identity
Score > 26 indicates homology		 TSGFEMVQGLLGK + Deamidated (NQ)
2504 538.2728 1611.7966 1611.7933 2.06 0 15 0.36 +1Score > 40 indicates identity
Score > 23 indicates homology		 ANFLAISGYIWNNK + 2 Deamidated (NQ)
3499 550.2773 2197.0801 2197.0666 6.13 0 15 1 +1Score > 39 indicates identity
Score > 27 indicates homology		 HNFSVWNMLLYFNSALPK + Deamidated (NQ); Oxidation (M)
3256 687.3583 2059.0531 2059.0143 18.8 1 15 0.59 +1Score > 39 indicates identity
Score > 25 indicates homology		 LMINTSTNEPDILVDEVR + Deamidated (NQ)
1491 381.8937 1142.6593 1142.6547 4.03 1 15 0.51 +1Score > 39 indicates identity
Score > 24 indicates homology		 KLALDLVDQK + Deamidated (NQ)
579 365.1989 728.3832 728.3817 2.13 0 15 0.74 +1Score > 39 indicates identity
Score > 26 indicates homology		 TQLPGGR + Deamidated (NQ)
1081 480.7917 959.5688 959.5804 -12.0 1 15 0.96 +1Score > 36 indicates identity
Score > 27 indicates homology		 LLVARFLE
604 372.7357 743.4568 743.4653 -11.4 1 15 0.9 +1Score > 41 indicates identity
Score > 27 indicates homology		 LGSRLAK
1330 537.8017 1073.5888 1073.5717 16.0 1 15 0.87 +1Score > 41 indicates identity
Score > 27 indicates homology		 QTVNVKDLR + 2 Deamidated (NQ)
2196 736.8682 1471.7218 1471.7266 -3.25 1 15 0.5 +1Score > 40 indicates identity
Score > 24 indicates homology		 QQINAIESANINR + 2 Deamidated (NQ)
2734 861.4301 1720.8456 1720.8342 6.67 0 15 0.35 +1Score > 41 indicates identity
Score > 23 indicates homology		 IVMDVANGATFNLNPK + 2 Deamidated (NQ); Oxidation (M)
1742 414.2474 1239.7204 1239.7339 -10.9 1 15 0.26 +1Score > 37 indicates identity
Score > 21 indicates homology		 HPSKLLAYALK
1876 654.8468 1307.6790 1307.6721 5.31 1 15 0.72 +1Score > 40 indicates identity
Score > 26 indicates homology		 LYELIDSNSVR
1921 664.3881 1326.7616 1326.7659 -3.24 1 15 0.35 +1Score > 37 indicates identity
Score > 23 indicates homology		 ISLDHLIFNKK
2446 529.2904 1584.8494 1584.8471 1.43 0 15 0.78 +1Score > 39 indicates identity
Score > 26 indicates homology		 LSAQQLQTNVNVLR + 2 Deamidated (NQ)
1546 584.8433 1167.6720 1167.6863 -12.2 1 15 0.93 +1Score > 37 indicates identity
Score > 27 indicates homology		 QEGLLLPSALK
1908 441.2407 1320.7003 1320.6781 16.8 1 15 0.61 +1Score > 40 indicates identity
Score > 25 indicates homology		 IGGLGVMAELMSK + Oxidation (M)
1736 413.8959 1238.6659 1238.6520 11.2 1 15 0.32 +1Score > 39 indicates identity
Score > 22 indicates homology		 HALKHYANGTK
3667 577.0305 2304.0929 2304.1096 -7.27 1 15 0.77 +1Score > 39 indicates identity
Score > 26 indicates homology		 VVTDKAPTDDQWDAMLLGWK + Oxidation (M)
1944 671.3814 1340.7482 1340.7551 -5.12 0 15 0.74 +1Score > 38 indicates identity
Score > 26 indicates homology		 LNPSLVISLSQGL + Deamidated (NQ)
2941 615.9759 1844.9059 1844.8839 11.9 0 15 0.47 +1Score > 40 indicates identity
Score > 24 indicates homology		 ANDGLFTAHCGVNITVR + Deamidated (NQ)
1214 339.4990 1015.4752 1015.4822 -6.90 1 15 0.55 +1Score > 40 indicates identity
Score > 24 indicates homology		 QPLNTGEQK + 2 Deamidated (NQ)
1200 506.3143 1010.6140 1010.6012 12.7 1 15 0.97 +1Score > 33 indicates identity
Score > 27 indicates homology		 VAELLIQKP + Deamidated (NQ)
1568 587.3133 1172.6120 1172.6336 -18.3 0 15 0.95 +1Score > 41 indicates identity
Score > 27 indicates homology		 AALILQTCQR
2254 500.5890 1498.7452 1498.7376 5.08 1 15 0.76 +1Score > 40 indicates identity
Score > 26 indicates homology		 QGGVEPDGNLSLRR + 2 Deamidated (NQ)
2461 797.4008 1592.7870 1592.7794 4.79 1 15 0.98 +1Score > 41 indicates identity
Score > 27 indicates homology		 SFPASNISLRSASQE
2093 471.5986 1411.7740 1411.7670 4.91 1 15 0.27 +1Score > 38 indicates identity
Score > 21 indicates homology		 SVAALPINDNIKR + 2 Deamidated (NQ)
3175 671.0005 2009.9797 2009.9847 -2.48 1 15 0.48 +1Score > 40 indicates identity
Score > 24 indicates homology		 LFDASGSARASLYFYNTK
3660 1152.0685 2302.1224 2302.1176 2.10 1 15 0.34 +1Score > 39 indicates identity
Score > 22 indicates homology		 SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
942 456.7456 911.4766 911.4898 -14.5 0 15 0.71 +1Score > 39 indicates identity
Score > 26 indicates homology		 LPNMALPR + Deamidated (NQ)
448 336.2271 670.4396 670.4377 2.84 0 14 0.4 +1Score > 37 indicates identity
Score > 23 indicates homology		 VAGAILK
1353 362.5463 1084.6171 1084.6063 9.97 1 14 0.62 +1Score > 39 indicates identity
Score > 25 indicates homology		 RPIMGAIAEK
1232 343.1934 1026.5584 1026.5498 8.32 1 14 0.97 +1Score > 39 indicates identity
Score > 27 indicates homology		 RVYDTFVK
2387 781.8811 1561.7476 1561.7770 -18.8 0 14 0.34 +1Score > 41 indicates identity
Score > 22 indicates homology		 GQNVGLIASLTTCAR + 2 Deamidated (NQ)
266 531.3268 530.3195 530.3176 3.58 0 14 0.28 +1Score > 46 indicates identity
Score > 21 indicates homology		 AITAR
1259 518.2993 1034.5840 1034.6012 -16.6 1 14 0.45 +1Score > 38 indicates identity
Score > 23 indicates homology		 IEIYIVGTK
2198 369.2047 1472.7897 1472.7796 6.86 1 14 0.95 +1Score > 40 indicates identity
Score > 27 indicates homology		 ISKMLLDSNPLLE + Deamidated (NQ)
3421 721.3817 2161.1233 2161.0903 15.3 0 14 0.35 +1Score > 38 indicates identity
Score > 22 indicates homology		 TDPYVTPGSVINNLSINTVR + 2 Deamidated (NQ)
3427 722.6939 2165.0599 2165.1005 -18.7 0 14 0.81 +1Score > 39 indicates identity
Score > 26 indicates homology		 VLSDVVASQAALHGPYGGVVPE + Deamidated (NQ)
881 440.2542 878.4938 878.4861 8.79 0 14 0.32 +1Score > 37 indicates identity
Score > 22 indicates homology		 YILNSIR + Deamidated (NQ)
612 375.7251 749.4356 749.4323 4.43 1 14 0.68 +1Score > 36 indicates identity
Score > 25 indicates homology		 FITLEK
382 315.7010 629.3874 629.3860 2.23 0 14 0.61 +1Score > 42 indicates identity
Score > 25 indicates homology		 ITAAVR
559 361.2168 720.4190 720.4283 -12.8 1 14 0.17 +1Score > 38 indicates identity
Score > 19 indicates homology		 VVYGKR
2319 510.6248 1528.8526 1528.8402 8.10 0 14 0.29 +1Score > 37 indicates identity
Score > 22 indicates homology		 AFNIDLGFPLIGPR
2353 774.3967 1546.7788 1546.7951 -10.5 1 14 0.85 +1Score > 41 indicates identity
Score > 26 indicates homology		 SSVDDINVLRVSSR + Deamidated (NQ)
2477 534.2759 1599.8059 1599.7749 19.4 1 14 0.41 +1Score > 41 indicates identity
Score > 23 indicates homology		 HGVQMMGQIIEALR + 2 Deamidated (NQ); Oxidation (M)
2053 700.3295 1398.6444 1398.6514 -4.98 0 14 0.93 +1Score > 40 indicates identity
Score > 27 indicates homology		 IIHQITDLNNSE + 3 Deamidated (NQ)
2758 866.8895 1731.7644 1731.7807 -9.41 0 14 0.24 +1Score > 38 indicates identity
Score > 21 indicates homology		 NDNPLVDMQAMSPTAK + Deamidated (NQ)
1729 619.8715 1237.7284 1237.7394 -8.86 0 14 0.91 +1Score > 34 indicates identity
Score > 26 indicates homology		 VAQALAVLTPVR + Deamidated (NQ)
655 385.2519 768.4892 768.4857 4.57 0 14 0.35 +1Score > 34 indicates identity
Score > 22 indicates homology		 AAAKPALK
752 412.7004 823.3862 823.3712 18.3 0 14 0.95 +1Score > 41 indicates identity
Score > 27 indicates homology		 SAGSYDPK
3180 671.3519 2011.0339 2011.0262 3.82 1 14 0.34 +1Score > 39 indicates identity
Score > 22 indicates homology		 QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
1242 1031.5144 1030.5071 1030.5196 -12.1 0 14 0.81 +1Score > 41 indicates identity
Score > 26 indicates homology		 NGVWSIAAGR + Deamidated (NQ)
1993 456.5704 1366.6894 1366.7133 -17.5 0 14 0.72 +1Score > 40 indicates identity
Score > 25 indicates homology		 VDYIQVADFIGK
1650 405.2039 1212.5899 1212.5696 16.7 0 14 0.71 +1Score > 41 indicates identity
Score > 25 indicates homology		 MLYQQIASNK + 2 Deamidated (NQ); Oxidation (M)
2360 775.4286 1548.8426 1548.8446 -1.26 1 14 0.82 +1Score > 38 indicates identity
Score > 26 indicates homology		 NLQYRDIVILMR + Oxidation (M)
2346 772.4261 1542.8376 1542.8439 -4.06 0 14 0.95 +1Score > 39 indicates identity
Score > 27 indicates homology		 NMVVSNILLNNIAK + Deamidated (NQ)
473 340.6805 679.3464 679.3442 3.32 0 14 0.67 +1Score > 40 indicates identity
Score > 25 indicates homology		 FPNFR
2293 379.2160 1512.8349 1512.8260 5.88 0 14 0.6 +1Score > 38 indicates identity
Score > 25 indicates homology		 LASNVNLSLGVVDGR
2848 597.3220 1788.9442 1788.9518 -4.25 1 14 0.53 +1Score > 39 indicates identity
Score > 24 indicates homology		 WMVVALVCLQLLEAK + Deamidated (NQ); Oxidation (M)
2926 612.3268 1833.9586 1833.9222 19.8 1 14 0.78 +1Score > 39 indicates identity
Score > 26 indicates homology		 SQGVIKLYIFYNMLE + Deamidated (NQ); Oxidation (M)
1429 374.5381 1120.5925 1120.5764 14.3 0 14 0.83 +1Score > 41 indicates identity
Score > 26 indicates homology		 KPSYNVGINK + 2 Deamidated (NQ)
3557 746.4081 2236.2025 2236.2150 -5.61 1 14 0.22 +1Score > 36 indicates identity
Score > 20 indicates homology		 MFLQARAALVTGGALYIVGNR + Oxidation (M)
1318 357.1592 1068.4558 1068.4764 -19.3 0 14 0.84 +1Score > 38 indicates identity
Score > 26 indicates homology		 QDIGFFDVE
2474 533.6340 1597.8802 1597.8675 7.91 0 14 0.22 +1Score > 38 indicates identity
Score > 20 indicates homology		 TVADLGSGNGSPGIILK
2195 736.8671 1471.7196 1471.7267 -4.76 1 14 0.48 +1Score > 40 indicates identity
Score > 24 indicates homology		 AAIGGEADQAITQAR + Deamidated (NQ)
2751 576.9913 1727.9521 1727.9206 18.2 1 14 0.24 +1Score > 37 indicates identity
Score > 21 indicates homology		 FNTLHQLLRTLQDK + 2 Deamidated (NQ)
2058 467.2788 1398.8146 1398.8082 4.55 1 14 0.48 +1Score > 35 indicates identity
Score > 24 indicates homology		 ISNVIKIPVSDSK
3055 642.3203 1923.9391 1923.9361 1.57 1 14 0.23 +1Score > 40 indicates identity
Score > 20 indicates homology		 DGTVRYDMTDLPVTAVR + Oxidation (M)
1639 602.8384 1203.6622 1203.6459 13.6 1 14 0.2 +1Score > 40 indicates identity
Score > 20 indicates homology		 SVSTARQLTLE
2399 523.2745 1566.8017 1566.7851 10.6 0 14 0.63 +1Score > 40 indicates identity
Score > 25 indicates homology		 MSVNVASLDFLLIE + Deamidated (NQ); Oxidation (M)
3932 848.7698 2543.2876 2543.2400 18.7 1 14 0.63 +1Score > 38 indicates identity
Score > 25 indicates homology		 QIYQMLFCGKNAQDVAISLLGR + 3 Deamidated (NQ); Oxidation (M)
1863 651.8625 1301.7104 1301.7013 7.04 1 14 0.88 +1Score > 40 indicates identity
Score > 26 indicates homology		 MGTNLAIEIALR + Deamidated (NQ)
1994 684.3525 1366.6904 1366.6803 7.46 1 14 0.8 +1Score > 40 indicates identity
Score > 26 indicates homology		 TSGFEMVQGLLGK + Deamidated (NQ)
2136 717.8653 1433.7160 1433.7191 -2.11 0 14 0.45 +1Score > 41 indicates identity
Score > 23 indicates homology		 LTNTSFNLYSFK
1743 621.4028 1240.7910 1240.8118 -16.7 1 14 0.15 +1Score > 24 indicates identity
Score > 18 indicates homology		 KISISLIGGLLK
2657 837.4750 1672.9354 1672.9070 17.0 1 14 0.6 +1Score > 36 indicates identity
Score > 24 indicates homology		 EKPTILLMVGVQGSGK + Deamidated (NQ); Oxidation (M)
1539 583.8329 1165.6512 1165.6342 14.6 1 14 0.86 +1Score > 37 indicates identity
Score > 26 indicates homology		 NAIGIELPNPK + Deamidated (NQ)
3040 638.9912 1913.9518 1913.9418 5.22 1 14 0.86 +1Score > 40 indicates identity
Score > 26 indicates homology		 NWLMQGVAAQGDRVQLE
764 415.2443 828.4740 828.4593 17.8 1 14 0.91 +1Score > 42 indicates identity
Score > 26 indicates homology		 AGEVVLLE
1815 427.2417 1278.7033 1278.6867 13.0 1 14 0.94 +1Score > 38 indicates identity
Score > 26 indicates homology		 SSPVLHRCVPK
305 586.3895 585.3822 585.3737 14.5 0 14 0.36 +1Score > 39 indicates identity
Score > 22 indicates homology		 ILDIL
1649 606.3555 1210.6964 1210.6809 12.8 1 14 0.28 +1Score > 36 indicates identity
Score > 21 indicates homology		 LVVLAKDVQPE + Deamidated (NQ)
2843 597.3120 1788.9142 1788.9444 -16.9 1 14 0.53 +1Score > 40 indicates identity
Score > 24 indicates homology		 AQVQHMVHLLLQLEK + 3 Deamidated (NQ)
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