MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3766 794.1039 2379.2899 2379.2885 0.56 0 16 0.089 +1Score > 34 indicates identity
Score > 18 indicates homology
FGIGADGVLFLAKPLHTSLHMR
1091 322.1759 963.5059 963.5025 3.48 1 16 0.92 +1Score > 42 indicates identity
Score > 28 indicates homology
SFAQAAIEK
2789 587.0001 1757.9785 1757.9828 -2.49 0 16 0.69 +1Score > 35 indicates identity
Score > 27 indicates homology
VSVIPTLLHVAGYSFR
2573 410.4501 1637.7713 1637.7640 4.43 1 16 0.12 +1Score > 40 indicates identity
Score > 19 indicates homology
MSNQELVTLNVGGMK + 2 Deamidated (NQ); Oxidation (M)
1797 637.8112 1273.6078 1273.5972 8.33 1 16 0.39 +1Score > 41 indicates identity
Score > 24 indicates homology
QPIMQGDTEQK
2352 515.6044 1543.7914 1543.7855 3.79 1 16 0.48 +1Score > 41 indicates identity
Score > 25 indicates homology
QAQRVGQPYVNQR + Deamidated (NQ)
2883 904.4634 1806.9122 1806.9220 -5.37 0 16 0.78 +1Score > 40 indicates identity
Score > 27 indicates homology
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M)
1407 557.8102 1113.6058 1113.6030 2.59 1 16 0.2 +1Score > 40 indicates identity
Score > 21 indicates homology
IAGTEAGISAPK
1381 551.3010 1100.5874 1100.5965 -8.22 1 16 0.46 +1Score > 41 indicates identity
Score > 25 indicates homology
LVLTDELVAE
1386 552.8312 1103.6478 1103.6260 19.8 1 16 0.77 +1Score > 35 indicates identity
Score > 27 indicates homology
KVVLLGMSLE + Oxidation (M)
3786 800.0901 2397.2485 2397.2217 11.2 1 16 0.12 +1Score > 37 indicates identity
Score > 19 indicates homology
ISWAKGGVLTTVVDDFFDVGGSK
3326 705.3888 2113.1446 2113.1380 3.10 0 16 0.11 +1Score > 36 indicates identity
Score > 19 indicates homology
WNQLDLAIVLLSIMGIALE + 2 Deamidated (NQ)
1157 332.8549 995.5429 995.5512 -8.39 1 16 0.45 +1Score > 37 indicates identity
Score > 25 indicates homology
DASVPPVRR
1810 639.8368 1277.6590 1277.6438 11.9 0 16 0.73 +1Score > 41 indicates identity
Score > 27 indicates homology
LLDIYPMQGGR + Oxidation (M)
1792 424.5707 1270.6903 1270.6670 18.3 1 16 0.25 +1Score > 40 indicates identity
Score > 22 indicates homology
ALEQPPYGVAAR
2451 794.4089 1586.8032 1586.7940 5.81 1 16 0.87 +1Score > 41 indicates identity
Score > 28 indicates homology
NYNDVVKTPAPAGLE
1740 414.2364 1239.6874 1239.7122 -20.0 1 16 0.77 +1Score > 38 indicates identity
Score > 27 indicates homology
VGRCLGAPIAVK
922 300.8199 899.4379 899.4349 3.35 0 16 0.67 +1Score > 39 indicates identity
Score > 27 indicates homology
GADALGPDGK
1657 607.8607 1213.7068 1213.7070 -0.15 0 16 0.59 +1Score > 37 indicates identity
Score > 26 indicates homology
LIGNIPLFAQK + Deamidated (NQ)
450 672.3809 671.3736 671.3602 19.9 0 16 0.8 +1Score > 41 indicates identity
Score > 27 indicates homology
DSPVVR
3178 671.0418 2010.1036 2010.0786 12.4 1 16 0.15 +1Score > 36 indicates identity
Score > 20 indicates homology
LVHDIIDLIYLNGLRNE + Deamidated (NQ)
420 652.3087 651.3014 651.3010 0.64 0 16 0.99 +1Score > 40 indicates identity
Score > 28 indicates homology
MATTGR + Oxidation (M)
2986 938.4541 1874.8936 1874.9110 -9.24 1 16 0.62 +1Score > 40 indicates identity
Score > 26 indicates homology
QIEVTTDGAGVSGTVVDVE
1688 408.5610 1222.6612 1222.6454 12.9 0 16 0.3 +1Score > 39 indicates identity
Score > 23 indicates homology
QISMIYPMLK
3034 478.5018 1909.9781 1909.9997 -11.3 0 16 0.59 +1Score > 39 indicates identity
Score > 26 indicates homology
IVSGGTDNHLLLVDLTNK + 2 Deamidated (NQ)
1096 322.8516 965.5330 965.5294 3.71 1 16 0.37 +1Score > 38 indicates identity
Score > 24 indicates homology
AIHNLLER + Deamidated (NQ)
697 395.7095 789.4044 789.3981 8.10 1 16 0.65 +1Score > 42 indicates identity
Score > 26 indicates homology
GAARLSSE
1076 479.8028 957.5910 957.5859 5.42 0 16 0.58 +1Score > 35 indicates identity
Score > 26 indicates homology
IILSGNTIK
1607 595.8452 1189.6758 1189.6859 -8.46 0 16 0.68 +1Score > 37 indicates identity
Score > 27 indicates homology
VISIAWNFLK
2557 408.6964 1630.7565 1630.7814 -15.2 1 16 0.25 +1Score > 40 indicates identity
Score > 22 indicates homology
CLAFGESFFLINGR + Deamidated (NQ)
1506 578.2928 1154.5710 1154.5641 5.99 0 16 0.2 +1Score > 40 indicates identity
Score > 21 indicates homology
QMIGYNLATK + Deamidated (NQ); Oxidation (M)
2115 712.3593 1422.7040 1422.6991 3.49 0 16 0.4 +1Score > 41 indicates identity
Score > 24 indicates homology
ATSVPGPTQATAPPE
734 407.2287 812.4428 812.4504 -9.32 1 16 0.72 +1Score > 39 indicates identity
Score > 27 indicates homology
KAPASSPR
1813 427.2405 1278.6997 1278.6932 5.09 1 16 0.53 +1Score > 38 indicates identity
Score > 25 indicates homology
LQAGLHQAIEAK + Deamidated (NQ)
3893 835.0878 2502.2416 2502.2446 -1.22 1 16 0.33 +1Score > 38 indicates identity
Score > 23 indicates homology
TAQMGWSLARLIAQMGWGAPAMR
896 444.2662 886.5178 886.5349 -19.2 1 16 0.96 +1Score > 40 indicates identity
Score > 28 indicates homology
VAVTVSRR
974 308.5166 922.5280 922.5348 -7.44 0 16 0.48 +1Score > 37 indicates identity
Score > 25 indicates homology
QLHSLLGR
326 301.6978 601.3810 601.3799 1.89 0 16 0.89 +1Score > 41 indicates identity
Score > 28 indicates homology
SVLVGK
2140 718.9128 1435.8110 1435.7922 13.1 1 16 0.36 +1Score > 35 indicates identity
Score > 24 indicates homology
AISKILDAISDYK
1598 594.8222 1187.6298 1187.6397 -8.32 1 16 0.75 +1Score > 41 indicates identity
Score > 27 indicates homology
LAEALTISQNK + Deamidated (NQ)
2463 532.2676 1593.7810 1593.7787 1.41 0 16 0.78 +1Score > 41 indicates identity
Score > 27 indicates homology
SFFDAPANQTVLQR + Deamidated (NQ)
2588 550.6289 1648.8649 1648.8533 7.04 1 16 0.16 +1Score > 39 indicates identity
Score > 20 indicates homology
LAELAQLQDQLDHR
2355 516.6047 1546.7923 1546.8215 -18.9 1 16 0.52 +1Score > 41 indicates identity
Score > 25 indicates homology
RNLNLDAHPIVQR + 2 Deamidated (NQ)
814 426.2118 850.4090 850.4106 -1.82 0 16 0.82 +1Score > 42 indicates identity
Score > 27 indicates homology
IQQMSVK + 2 Deamidated (NQ); Oxidation (M)
1268 347.8553 1040.5441 1040.5403 3.60 0 16 0.93 +1Score > 39 indicates identity
Score > 28 indicates homology
DLFSPHLGR
1424 560.8060 1119.5974 1119.6111 -12.2 1 16 0.99 +1Score > 41 indicates identity
Score > 28 indicates homology
KVGFPSVVCK
1439 564.7867 1127.5588 1127.5458 11.5 1 16 0.78 +1Score > 40 indicates identity
Score > 27 indicates homology
QAAEALQPTAE
1949 672.8523 1343.6900 1343.6905 -0.36 1 16 0.56 +1Score > 41 indicates identity
Score > 26 indicates homology
ILSSNGRNVNNR + Deamidated (NQ)
643 382.2098 762.4050 762.3945 13.8 0 16 0.71 +1Score > 43 indicates identity
Score > 26 indicates homology
MILNAGK + Deamidated (NQ); Oxidation (M)
2475 799.9487 1597.8828 1597.8787 2.57 1 16 0.49 +1Score > 37 indicates identity
Score > 25 indicates homology
TIINDIQRVSKPGR + 2 Deamidated (NQ)
1061 319.5149 955.5229 955.5312 -8.67 1 15 0.54 +1Score > 39 indicates identity
Score > 25 indicates homology
GRGNAQVVR
1990 456.5700 1366.6882 1366.6915 -2.42 0 15 0.82 +1Score > 40 indicates identity
Score > 27 indicates homology
GTYVCIATNALGK
786 421.7232 841.4318 841.4406 -10.4 0 15 0.36 +1Score > 39 indicates identity
Score > 24 indicates homology
NTPNQIR
1762 627.2924 1252.5702 1252.5791 -7.10 1 15 0.84 +1Score > 40 indicates identity
Score > 27 indicates homology
SVSELMDGMLR + Oxidation (M)
2888 904.9623 1807.9100 1807.9203 -5.69 0 15 0.24 +1Score > 40 indicates identity
Score > 22 indicates homology
AQVDALLNSPNVDIPLE + Deamidated (NQ)
3331 706.0323 2115.0751 2115.0445 14.4 0 15 0.48 +1Score > 39 indicates identity
Score > 25 indicates homology
MTSPIPVTLLIHYQLNQE + 3 Deamidated (NQ); Oxidation (M)
4471 1093.5566 3277.6480 3277.6024 13.9 1 15 0.06 +1Score > 35 indicates identity
Score > 16 indicates homology
DNSQPTPDNVLSAVTPELINLAGAGIPPMSTR + 3 Deamidated (NQ)
2345 772.4161 1542.8176 1542.8140 2.34 0 15 0.5 +1Score > 40 indicates identity
Score > 25 indicates homology
LAIQNSQIASAIALE + 2 Deamidated (NQ)
1867 653.8424 1305.6702 1305.6565 10.5 1 15 0.19 +1Score > 41 indicates identity
Score > 21 indicates homology
LFSEQGNVVDAK
2872 901.9128 1801.8110 1801.8442 -18.4 1 15 0.81 +1Score > 39 indicates identity
Score > 27 indicates homology
RDLTINAIAQDDQGNR + 3 Deamidated (NQ)
781 419.6916 837.3686 837.3538 17.7 0 15 0.91 +1Score > 40 indicates identity
Score > 27 indicates homology
NAMDTAAK + Deamidated (NQ); Oxidation (M)
2200 737.4061 1472.7976 1472.7947 2.01 1 15 0.78 +1Score > 39 indicates identity
Score > 27 indicates homology
SSTPLQTVASAVRR + Deamidated (NQ)
2522 807.9292 1613.8438 1613.8373 4.07 1 15 0.34 +1Score > 40 indicates identity
Score > 23 indicates homology
QAADDPLNTVSSIKR
3181 1006.5279 2011.0412 2011.0473 -3.03 1 15 0.67 +1Score > 39 indicates identity
Score > 26 indicates homology
GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ)
397 637.3322 636.3249 636.3231 2.86 1 15 0.94 +1Score > 41 indicates identity
Score > 28 indicates homology
EVAYR
506 348.7068 695.3990 695.3966 3.53 0 15 0.62 +1Score > 36 indicates identity
Score > 26 indicates homology
APAAPAAK
1817 640.8530 1279.6914 1279.6673 18.9 1 15 0.96 +1Score > 39 indicates identity
Score > 28 indicates homology
NPYLANRYLR + Deamidated (NQ)
2592 825.9329 1649.8512 1649.8334 10.8 1 15 0.56 +1Score > 40 indicates identity
Score > 25 indicates homology
AVLLMAAFELTQQAE + Oxidation (M)
3013 946.4976 1890.9806 1891.0163 -18.9 1 15 0.84 +1Score > 39 indicates identity
Score > 27 indicates homology
VQQAGELQNGTLVHGVLK + Deamidated (NQ)
3913 631.8043 2523.1881 2523.2099 -8.66 0 15 0.066 +1Score > 38 indicates identity
Score > 16 indicates homology
TMPFPGFPTDLQPLMMSLLATAE + Oxidation (M)
986 464.7115 927.4084 927.3974 11.9 1 15 0.61 +1Score > 38 indicates identity
Score > 26 indicates homology
EFFDQNK + Deamidated (NQ)
1882 656.3750 1310.7354 1310.7558 -15.5 0 15 0.84 +1Score > 37 indicates identity
Score > 27 indicates homology
IAVPLLSDINTR
1263 346.8507 1037.5303 1037.5142 15.5 0 15 0.91 +1Score > 40 indicates identity
Score > 27 indicates homology
FSIGLSSDGR
2987 626.0135 1875.0187 1875.0288 -5.41 1 15 0.29 +1Score > 36 indicates identity
Score > 22 indicates homology
RPFGVAVMDITDIIKGK + Oxidation (M)
343 307.6795 613.3444 613.3435 1.49 0 15 0.76 +1Score > 37 indicates identity
Score > 26 indicates homology
VGPDVK
865 437.2160 872.4174 872.4100 8.52 0 15 0.5 +1Score > 39 indicates identity
Score > 25 indicates homology
QAQNQQR + Deamidated (NQ)
1763 628.3198 1254.6250 1254.6318 -5.41 0 15 0.099 +1Score > 40 indicates identity
Score > 18 indicates homology
VLFCYIKPSE
1898 659.8970 1317.7794 1317.7656 10.5 1 15 0.66 +1Score > 32 indicates identity
Score > 26 indicates homology
KLSVAIAFVGDAK
3889 832.4509 2494.3309 2494.3067 9.68 1 15 0.12 +1Score > 35 indicates identity
Score > 19 indicates homology
TSAYIDVPTLVRNTILDIGYNR + Deamidated (NQ)
1695 410.2279 1227.6619 1227.6684 -5.30 1 15 0.74 +1Score > 40 indicates identity
Score > 26 indicates homology
GVDLAQARVSGR
3182 671.3553 2011.0441 2011.0262 8.89 1 15 0.59 +1Score > 39 indicates identity
Score > 25 indicates homology
QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
2054 700.3312 1398.6478 1398.6514 -2.55 0 15 0.98 +1Score > 40 indicates identity
Score > 28 indicates homology
IIHQITDLNNSE + 3 Deamidated (NQ)
1125 491.7402 981.4658 981.4477 18.5 0 15 0.94 +1Score > 41 indicates identity
Score > 27 indicates homology
QFVQAMIE + Deamidated (NQ); Oxidation (M)
2513 806.9459 1611.8772 1611.8468 18.9 1 15 0.82 +1Score > 38 indicates identity
Score > 27 indicates homology
VVLVNNILQNAQER + 3 Deamidated (NQ)
788 421.7233 841.4320 841.4294 3.20 0 15 0.4 +1Score > 39 indicates identity
Score > 24 indicates homology
NPDNLIR + Deamidated (NQ)
2050 699.8882 1397.7618 1397.7514 7.47 1 15 0.58 +1Score > 39 indicates identity
Score > 25 indicates homology
VLALLNDNLQRE + Deamidated (NQ)
3322 704.3737 2110.0993 2110.0921 3.40 1 15 0.78 +1Score > 38 indicates identity
Score > 27 indicates homology
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
3498 550.2763 2197.0761 2197.0976 -9.81 1 15 0.77 +1Score > 39 indicates identity
Score > 26 indicates homology
YYPLQATTLLIQPMEATAAS + Oxidation (M)
529 351.7082 701.4018 701.3959 8.41 1 15 0.99 +1Score > 43 indicates identity
Score > 28 indicates homology
IDEVVK
1297 353.5283 1057.5631 1057.5768 -12.9 1 15 0.16 +1Score > 42 indicates identity
Score > 20 indicates homology
KAQQVLGVSE
1563 586.8543 1171.6940 1171.6812 11.0 1 15 0.55 +1Score > 36 indicates identity
Score > 25 indicates homology
LTLKLQTLNE
2246 748.9010 1495.7874 1495.8021 -9.80 1 15 0.88 +1Score > 39 indicates identity
Score > 27 indicates homology
IPIELLQVNDIVE + 2 Deamidated (NQ)
999 466.2479 930.4812 930.4883 -7.58 1 15 0.69 +1Score > 42 indicates identity
Score > 26 indicates homology
LTGDRVDR
1349 542.7885 1083.5624 1083.5560 5.92 0 15 0.51 +1Score > 40 indicates identity
Score > 25 indicates homology
GSAPGQPVTAAK + Deamidated (NQ)
634 380.7247 759.4348 759.4279 9.15 0 15 0.97 +1Score > 41 indicates identity
Score > 27 indicates homology
WNSIIK
1744 414.8919 1241.6539 1241.6616 -6.19 0 15 0.45 +1Score > 40 indicates identity
Score > 24 indicates homology
TVQPTSPATALR + Deamidated (NQ)
3551 1117.6467 2233.2788 2233.2583 9.21 1 15 0.66 +1Score > 29 indicates identity
Score > 26 indicates homology
SLTRFGFTLIIGGAIGNLIDR
2983 936.4662 1870.9178 1870.9061 6.29 1 15 0.59 +1Score > 40 indicates identity
Score > 25 indicates homology
AAVHELSDYLIGQDPSR + Deamidated (NQ)
1911 331.1999 1320.7705 1320.7765 -4.56 1 15 0.3 +1Score > 33 indicates identity
Score > 22 indicates homology
GIKDLHDILGLK
533 352.2046 702.3946 702.3912 4.90 0 15 0.8 +1Score > 43 indicates identity
Score > 27 indicates homology
GGLDLTK
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