| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 794.1039 | 2379.2899 | 2379.2885 | 0.56 | 0 | 16 | 0.089 | 1Score > 34 indicates identityScore > 18 indicates homology |
FGIGADGVLFLAKPLHTSLHMR | |
| 322.1759 | 963.5059 | 963.5025 | 3.48 | 1 | 16 | 0.92 | 1Score > 42 indicates identityScore > 28 indicates homology |
SFAQAAIEK | |
| 587.0001 | 1757.9785 | 1757.9828 | -2.49 | 0 | 16 | 0.69 | 1Score > 35 indicates identityScore > 27 indicates homology |
VSVIPTLLHVAGYSFR | |
| 410.4501 | 1637.7713 | 1637.7640 | 4.43 | 1 | 16 | 0.12 | 1Score > 40 indicates identityScore > 19 indicates homology |
MSNQELVTLNVGGMK + 2 Deamidated (NQ); Oxidation (M) | |
| 637.8112 | 1273.6078 | 1273.5972 | 8.33 | 1 | 16 | 0.39 | 1Score > 41 indicates identityScore > 24 indicates homology |
QPIMQGDTEQK | |
| 515.6044 | 1543.7914 | 1543.7855 | 3.79 | 1 | 16 | 0.48 | 1Score > 41 indicates identityScore > 25 indicates homology |
QAQRVGQPYVNQR + Deamidated (NQ) | |
| 904.4634 | 1806.9122 | 1806.9220 | -5.37 | 0 | 16 | 0.78 | 1Score > 40 indicates identityScore > 27 indicates homology |
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M) | |
| 557.8102 | 1113.6058 | 1113.6030 | 2.59 | 1 | 16 | 0.2 | 1Score > 40 indicates identityScore > 21 indicates homology |
IAGTEAGISAPK | |
| 551.3010 | 1100.5874 | 1100.5965 | -8.22 | 1 | 16 | 0.46 | 1Score > 41 indicates identityScore > 25 indicates homology |
LVLTDELVAE | |
| 552.8312 | 1103.6478 | 1103.6260 | 19.8 | 1 | 16 | 0.77 | 1Score > 35 indicates identityScore > 27 indicates homology |
KVVLLGMSLE + Oxidation (M) | |
| 800.0901 | 2397.2485 | 2397.2217 | 11.2 | 1 | 16 | 0.12 | 1Score > 37 indicates identityScore > 19 indicates homology |
ISWAKGGVLTTVVDDFFDVGGSK | |
| 705.3888 | 2113.1446 | 2113.1380 | 3.10 | 0 | 16 | 0.11 | 1Score > 36 indicates identityScore > 19 indicates homology |
WNQLDLAIVLLSIMGIALE + 2 Deamidated (NQ) | |
| 332.8549 | 995.5429 | 995.5512 | -8.39 | 1 | 16 | 0.45 | 1Score > 37 indicates identityScore > 25 indicates homology |
DASVPPVRR | |
| 639.8368 | 1277.6590 | 1277.6438 | 11.9 | 0 | 16 | 0.73 | 1Score > 41 indicates identityScore > 27 indicates homology |
LLDIYPMQGGR + Oxidation (M) | |
| 424.5707 | 1270.6903 | 1270.6670 | 18.3 | 1 | 16 | 0.25 | 1Score > 40 indicates identityScore > 22 indicates homology |
ALEQPPYGVAAR | |
| 794.4089 | 1586.8032 | 1586.7940 | 5.81 | 1 | 16 | 0.87 | 1Score > 41 indicates identityScore > 28 indicates homology |
NYNDVVKTPAPAGLE | |
| 414.2364 | 1239.6874 | 1239.7122 | -20.0 | 1 | 16 | 0.77 | 1Score > 38 indicates identityScore > 27 indicates homology |
VGRCLGAPIAVK | |
| 300.8199 | 899.4379 | 899.4349 | 3.35 | 0 | 16 | 0.67 | 1Score > 39 indicates identityScore > 27 indicates homology |
GADALGPDGK | |
| 607.8607 | 1213.7068 | 1213.7070 | -0.15 | 0 | 16 | 0.59 | 1Score > 37 indicates identityScore > 26 indicates homology |
LIGNIPLFAQK + Deamidated (NQ) | |
| 672.3809 | 671.3736 | 671.3602 | 19.9 | 0 | 16 | 0.8 | 1Score > 41 indicates identityScore > 27 indicates homology |
DSPVVR | |
| 671.0418 | 2010.1036 | 2010.0786 | 12.4 | 1 | 16 | 0.15 | 1Score > 36 indicates identityScore > 20 indicates homology |
LVHDIIDLIYLNGLRNE + Deamidated (NQ) | |
| 652.3087 | 651.3014 | 651.3010 | 0.64 | 0 | 16 | 0.99 | 1Score > 40 indicates identityScore > 28 indicates homology |
MATTGR + Oxidation (M) | |
| 938.4541 | 1874.8936 | 1874.9110 | -9.24 | 1 | 16 | 0.62 | 1Score > 40 indicates identityScore > 26 indicates homology |
QIEVTTDGAGVSGTVVDVE | |
| 408.5610 | 1222.6612 | 1222.6454 | 12.9 | 0 | 16 | 0.3 | 1Score > 39 indicates identityScore > 23 indicates homology |
QISMIYPMLK | |
| 478.5018 | 1909.9781 | 1909.9997 | -11.3 | 0 | 16 | 0.59 | 1Score > 39 indicates identityScore > 26 indicates homology |
IVSGGTDNHLLLVDLTNK + 2 Deamidated (NQ) | |
| 322.8516 | 965.5330 | 965.5294 | 3.71 | 1 | 16 | 0.37 | 1Score > 38 indicates identityScore > 24 indicates homology |
AIHNLLER + Deamidated (NQ) | |
| 395.7095 | 789.4044 | 789.3981 | 8.10 | 1 | 16 | 0.65 | 1Score > 42 indicates identityScore > 26 indicates homology |
GAARLSSE | |
| 479.8028 | 957.5910 | 957.5859 | 5.42 | 0 | 16 | 0.58 | 1Score > 35 indicates identityScore > 26 indicates homology |
IILSGNTIK | |
| 595.8452 | 1189.6758 | 1189.6859 | -8.46 | 0 | 16 | 0.68 | 1Score > 37 indicates identityScore > 27 indicates homology |
VISIAWNFLK | |
| 408.6964 | 1630.7565 | 1630.7814 | -15.2 | 1 | 16 | 0.25 | 1Score > 40 indicates identityScore > 22 indicates homology |
CLAFGESFFLINGR + Deamidated (NQ) | |
| 578.2928 | 1154.5710 | 1154.5641 | 5.99 | 0 | 16 | 0.2 | 1Score > 40 indicates identityScore > 21 indicates homology |
QMIGYNLATK + Deamidated (NQ); Oxidation (M) | |
| 712.3593 | 1422.7040 | 1422.6991 | 3.49 | 0 | 16 | 0.4 | 1Score > 41 indicates identityScore > 24 indicates homology |
ATSVPGPTQATAPPE | |
| 407.2287 | 812.4428 | 812.4504 | -9.32 | 1 | 16 | 0.72 | 1Score > 39 indicates identityScore > 27 indicates homology |
KAPASSPR | |
| 427.2405 | 1278.6997 | 1278.6932 | 5.09 | 1 | 16 | 0.53 | 1Score > 38 indicates identityScore > 25 indicates homology |
LQAGLHQAIEAK + Deamidated (NQ) | |
| 835.0878 | 2502.2416 | 2502.2446 | -1.22 | 1 | 16 | 0.33 | 1Score > 38 indicates identityScore > 23 indicates homology |
TAQMGWSLARLIAQMGWGAPAMR | |
| 444.2662 | 886.5178 | 886.5349 | -19.2 | 1 | 16 | 0.96 | 1Score > 40 indicates identityScore > 28 indicates homology |
VAVTVSRR | |
| 308.5166 | 922.5280 | 922.5348 | -7.44 | 0 | 16 | 0.48 | 1Score > 37 indicates identityScore > 25 indicates homology |
QLHSLLGR | |
| 301.6978 | 601.3810 | 601.3799 | 1.89 | 0 | 16 | 0.89 | 1Score > 41 indicates identityScore > 28 indicates homology |
SVLVGK | |
| 718.9128 | 1435.8110 | 1435.7922 | 13.1 | 1 | 16 | 0.36 | 1Score > 35 indicates identityScore > 24 indicates homology |
AISKILDAISDYK | |
| 594.8222 | 1187.6298 | 1187.6397 | -8.32 | 1 | 16 | 0.75 | 1Score > 41 indicates identityScore > 27 indicates homology |
LAEALTISQNK + Deamidated (NQ) | |
| 532.2676 | 1593.7810 | 1593.7787 | 1.41 | 0 | 16 | 0.78 | 1Score > 41 indicates identityScore > 27 indicates homology |
SFFDAPANQTVLQR + Deamidated (NQ) | |
| 550.6289 | 1648.8649 | 1648.8533 | 7.04 | 1 | 16 | 0.16 | 1Score > 39 indicates identityScore > 20 indicates homology |
LAELAQLQDQLDHR | |
| 516.6047 | 1546.7923 | 1546.8215 | -18.9 | 1 | 16 | 0.52 | 1Score > 41 indicates identityScore > 25 indicates homology |
RNLNLDAHPIVQR + 2 Deamidated (NQ) | |
| 426.2118 | 850.4090 | 850.4106 | -1.82 | 0 | 16 | 0.82 | 1Score > 42 indicates identityScore > 27 indicates homology |
IQQMSVK + 2 Deamidated (NQ); Oxidation (M) | |
| 347.8553 | 1040.5441 | 1040.5403 | 3.60 | 0 | 16 | 0.93 | 1Score > 39 indicates identityScore > 28 indicates homology |
DLFSPHLGR | |
| 560.8060 | 1119.5974 | 1119.6111 | -12.2 | 1 | 16 | 0.99 | 1Score > 41 indicates identityScore > 28 indicates homology |
KVGFPSVVCK | |
| 564.7867 | 1127.5588 | 1127.5458 | 11.5 | 1 | 16 | 0.78 | 1Score > 40 indicates identityScore > 27 indicates homology |
QAAEALQPTAE | |
| 672.8523 | 1343.6900 | 1343.6905 | -0.36 | 1 | 16 | 0.56 | 1Score > 41 indicates identityScore > 26 indicates homology |
ILSSNGRNVNNR + Deamidated (NQ) | |
| 382.2098 | 762.4050 | 762.3945 | 13.8 | 0 | 16 | 0.71 | 1Score > 43 indicates identityScore > 26 indicates homology |
MILNAGK + Deamidated (NQ); Oxidation (M) | |
| 799.9487 | 1597.8828 | 1597.8787 | 2.57 | 1 | 16 | 0.49 | 1Score > 37 indicates identityScore > 25 indicates homology |
TIINDIQRVSKPGR + 2 Deamidated (NQ) | |
| 319.5149 | 955.5229 | 955.5312 | -8.67 | 1 | 15 | 0.54 | 1Score > 39 indicates identityScore > 25 indicates homology |
GRGNAQVVR | |
| 456.5700 | 1366.6882 | 1366.6915 | -2.42 | 0 | 15 | 0.82 | 1Score > 40 indicates identityScore > 27 indicates homology |
GTYVCIATNALGK | |
| 421.7232 | 841.4318 | 841.4406 | -10.4 | 0 | 15 | 0.36 | 1Score > 39 indicates identityScore > 24 indicates homology |
NTPNQIR | |
| 627.2924 | 1252.5702 | 1252.5791 | -7.10 | 1 | 15 | 0.84 | 1Score > 40 indicates identityScore > 27 indicates homology |
SVSELMDGMLR + Oxidation (M) | |
| 904.9623 | 1807.9100 | 1807.9203 | -5.69 | 0 | 15 | 0.24 | 1Score > 40 indicates identityScore > 22 indicates homology |
AQVDALLNSPNVDIPLE + Deamidated (NQ) | |
| 706.0323 | 2115.0751 | 2115.0445 | 14.4 | 0 | 15 | 0.48 | 1Score > 39 indicates identityScore > 25 indicates homology |
MTSPIPVTLLIHYQLNQE + 3 Deamidated (NQ); Oxidation (M) | |
| 1093.5566 | 3277.6480 | 3277.6024 | 13.9 | 1 | 15 | 0.06 | 1Score > 35 indicates identityScore > 16 indicates homology |
DNSQPTPDNVLSAVTPELINLAGAGIPPMSTR + 3 Deamidated (NQ) | |
| 772.4161 | 1542.8176 | 1542.8140 | 2.34 | 0 | 15 | 0.5 | 1Score > 40 indicates identityScore > 25 indicates homology |
LAIQNSQIASAIALE + 2 Deamidated (NQ) | |
| 653.8424 | 1305.6702 | 1305.6565 | 10.5 | 1 | 15 | 0.19 | 1Score > 41 indicates identityScore > 21 indicates homology |
LFSEQGNVVDAK | |
| 901.9128 | 1801.8110 | 1801.8442 | -18.4 | 1 | 15 | 0.81 | 1Score > 39 indicates identityScore > 27 indicates homology |
RDLTINAIAQDDQGNR + 3 Deamidated (NQ) | |
| 419.6916 | 837.3686 | 837.3538 | 17.7 | 0 | 15 | 0.91 | 1Score > 40 indicates identityScore > 27 indicates homology |
NAMDTAAK + Deamidated (NQ); Oxidation (M) | |
| 737.4061 | 1472.7976 | 1472.7947 | 2.01 | 1 | 15 | 0.78 | 1Score > 39 indicates identityScore > 27 indicates homology |
SSTPLQTVASAVRR + Deamidated (NQ) | |
| 807.9292 | 1613.8438 | 1613.8373 | 4.07 | 1 | 15 | 0.34 | 1Score > 40 indicates identityScore > 23 indicates homology |
QAADDPLNTVSSIKR | |
| 1006.5279 | 2011.0412 | 2011.0473 | -3.03 | 1 | 15 | 0.67 | 1Score > 39 indicates identityScore > 26 indicates homology |
GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ) | |
| 637.3322 | 636.3249 | 636.3231 | 2.86 | 1 | 15 | 0.94 | 1Score > 41 indicates identityScore > 28 indicates homology |
EVAYR | |
| 348.7068 | 695.3990 | 695.3966 | 3.53 | 0 | 15 | 0.62 | 1Score > 36 indicates identityScore > 26 indicates homology |
APAAPAAK | |
| 640.8530 | 1279.6914 | 1279.6673 | 18.9 | 1 | 15 | 0.96 | 1Score > 39 indicates identityScore > 28 indicates homology |
NPYLANRYLR + Deamidated (NQ) | |
| 825.9329 | 1649.8512 | 1649.8334 | 10.8 | 1 | 15 | 0.56 | 1Score > 40 indicates identityScore > 25 indicates homology |
AVLLMAAFELTQQAE + Oxidation (M) | |
| 946.4976 | 1890.9806 | 1891.0163 | -18.9 | 1 | 15 | 0.84 | 1Score > 39 indicates identityScore > 27 indicates homology |
VQQAGELQNGTLVHGVLK + Deamidated (NQ) | |
| 631.8043 | 2523.1881 | 2523.2099 | -8.66 | 0 | 15 | 0.066 | 1Score > 38 indicates identityScore > 16 indicates homology |
TMPFPGFPTDLQPLMMSLLATAE + Oxidation (M) | |
| 464.7115 | 927.4084 | 927.3974 | 11.9 | 1 | 15 | 0.61 | 1Score > 38 indicates identityScore > 26 indicates homology |
EFFDQNK + Deamidated (NQ) | |
| 656.3750 | 1310.7354 | 1310.7558 | -15.5 | 0 | 15 | 0.84 | 1Score > 37 indicates identityScore > 27 indicates homology |
IAVPLLSDINTR | |
| 346.8507 | 1037.5303 | 1037.5142 | 15.5 | 0 | 15 | 0.91 | 1Score > 40 indicates identityScore > 27 indicates homology |
FSIGLSSDGR | |
| 626.0135 | 1875.0187 | 1875.0288 | -5.41 | 1 | 15 | 0.29 | 1Score > 36 indicates identityScore > 22 indicates homology |
RPFGVAVMDITDIIKGK + Oxidation (M) | |
| 307.6795 | 613.3444 | 613.3435 | 1.49 | 0 | 15 | 0.76 | 1Score > 37 indicates identityScore > 26 indicates homology |
VGPDVK | |
| 437.2160 | 872.4174 | 872.4100 | 8.52 | 0 | 15 | 0.5 | 1Score > 39 indicates identityScore > 25 indicates homology |
QAQNQQR + Deamidated (NQ) | |
| 628.3198 | 1254.6250 | 1254.6318 | -5.41 | 0 | 15 | 0.099 | 1Score > 40 indicates identityScore > 18 indicates homology |
VLFCYIKPSE | |
| 659.8970 | 1317.7794 | 1317.7656 | 10.5 | 1 | 15 | 0.66 | 1Score > 32 indicates identityScore > 26 indicates homology |
KLSVAIAFVGDAK | |
| 832.4509 | 2494.3309 | 2494.3067 | 9.68 | 1 | 15 | 0.12 | 1Score > 35 indicates identityScore > 19 indicates homology |
TSAYIDVPTLVRNTILDIGYNR + Deamidated (NQ) | |
| 410.2279 | 1227.6619 | 1227.6684 | -5.30 | 1 | 15 | 0.74 | 1Score > 40 indicates identityScore > 26 indicates homology |
GVDLAQARVSGR | |
| 671.3553 | 2011.0441 | 2011.0262 | 8.89 | 1 | 15 | 0.59 | 1Score > 39 indicates identityScore > 25 indicates homology |
QTLSPSVLIEHLNLFGNE + Deamidated (NQ) | |
| 700.3312 | 1398.6478 | 1398.6514 | -2.55 | 0 | 15 | 0.98 | 1Score > 40 indicates identityScore > 28 indicates homology |
IIHQITDLNNSE + 3 Deamidated (NQ) | |
| 491.7402 | 981.4658 | 981.4477 | 18.5 | 0 | 15 | 0.94 | 1Score > 41 indicates identityScore > 27 indicates homology |
QFVQAMIE + Deamidated (NQ); Oxidation (M) | |
| 806.9459 | 1611.8772 | 1611.8468 | 18.9 | 1 | 15 | 0.82 | 1Score > 38 indicates identityScore > 27 indicates homology |
VVLVNNILQNAQER + 3 Deamidated (NQ) | |
| 421.7233 | 841.4320 | 841.4294 | 3.20 | 0 | 15 | 0.4 | 1Score > 39 indicates identityScore > 24 indicates homology |
NPDNLIR + Deamidated (NQ) | |
| 699.8882 | 1397.7618 | 1397.7514 | 7.47 | 1 | 15 | 0.58 | 1Score > 39 indicates identityScore > 25 indicates homology |
VLALLNDNLQRE + Deamidated (NQ) | |
| 704.3737 | 2110.0993 | 2110.0921 | 3.40 | 1 | 15 | 0.78 | 1Score > 38 indicates identityScore > 27 indicates homology |
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M) | |
| 550.2763 | 2197.0761 | 2197.0976 | -9.81 | 1 | 15 | 0.77 | 1Score > 39 indicates identityScore > 26 indicates homology |
YYPLQATTLLIQPMEATAAS + Oxidation (M) | |
| 351.7082 | 701.4018 | 701.3959 | 8.41 | 1 | 15 | 0.99 | 1Score > 43 indicates identityScore > 28 indicates homology |
IDEVVK | |
| 353.5283 | 1057.5631 | 1057.5768 | -12.9 | 1 | 15 | 0.16 | 1Score > 42 indicates identityScore > 20 indicates homology |
KAQQVLGVSE | |
| 586.8543 | 1171.6940 | 1171.6812 | 11.0 | 1 | 15 | 0.55 | 1Score > 36 indicates identityScore > 25 indicates homology |
LTLKLQTLNE | |
| 748.9010 | 1495.7874 | 1495.8021 | -9.80 | 1 | 15 | 0.88 | 1Score > 39 indicates identityScore > 27 indicates homology |
IPIELLQVNDIVE + 2 Deamidated (NQ) | |
| 466.2479 | 930.4812 | 930.4883 | -7.58 | 1 | 15 | 0.69 | 1Score > 42 indicates identityScore > 26 indicates homology |
LTGDRVDR | |
| 542.7885 | 1083.5624 | 1083.5560 | 5.92 | 0 | 15 | 0.51 | 1Score > 40 indicates identityScore > 25 indicates homology |
GSAPGQPVTAAK + Deamidated (NQ) | |
| 380.7247 | 759.4348 | 759.4279 | 9.15 | 0 | 15 | 0.97 | 1Score > 41 indicates identityScore > 27 indicates homology |
WNSIIK | |
| 414.8919 | 1241.6539 | 1241.6616 | -6.19 | 0 | 15 | 0.45 | 1Score > 40 indicates identityScore > 24 indicates homology |
TVQPTSPATALR + Deamidated (NQ) | |
| 1117.6467 | 2233.2788 | 2233.2583 | 9.21 | 1 | 15 | 0.66 | 1Score > 29 indicates identityScore > 26 indicates homology |
SLTRFGFTLIIGGAIGNLIDR | |
| 936.4662 | 1870.9178 | 1870.9061 | 6.29 | 1 | 15 | 0.59 | 1Score > 40 indicates identityScore > 25 indicates homology |
AAVHELSDYLIGQDPSR + Deamidated (NQ) | |
| 331.1999 | 1320.7705 | 1320.7765 | -4.56 | 1 | 15 | 0.3 | 1Score > 33 indicates identityScore > 22 indicates homology |
GIKDLHDILGLK | |
| 352.2046 | 702.3946 | 702.3912 | 4.90 | 0 | 15 | 0.8 | 1Score > 43 indicates identityScore > 27 indicates homology |
GGLDLTK |
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