| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 471.2875 | 1410.8407 | 1410.8194 | 15.1 | 1 | 17 | 0.56 | 1Score > 33 indicates identityScore > 27 indicates homology |
DLKQQLLLLNGR + Deamidated (NQ) | |
| 781.4021 | 1560.7896 | 1560.8082 | -11.9 | 0 | 17 | 0.84 | 1Score > 41 indicates identityScore > 29 indicates homology |
MIVFVNQRPVDSR + Deamidated (NQ) | |
| 560.2787 | 1118.5428 | 1118.5356 | 6.47 | 1 | 17 | 0.98 | 1Score > 42 indicates identityScore > 30 indicates homology |
QANQSEFAPK | |
| 589.2988 | 1176.5830 | 1176.6060 | -19.5 | 1 | 17 | 0.59 | 1Score > 42 indicates identityScore > 28 indicates homology |
ALAKLDISAME + Oxidation (M) | |
| 347.2237 | 1038.6493 | 1038.6477 | 1.49 | 1 | 17 | 0.98 | 1Score > 33 indicates identityScore > 30 indicates homology |
LYIYKVLK | |
| 660.3090 | 1318.6034 | 1318.6153 | -9.02 | 0 | 17 | 0.094 | 1Score > 40 indicates identityScore > 20 indicates homology |
TLDISDADWQR | |
| 711.3491 | 1420.6836 | 1420.6834 | 0.18 | 1 | 17 | 0.77 | 1Score > 41 indicates identityScore > 29 indicates homology |
INQADGAVDKFNK + 2 Deamidated (NQ) | |
| 322.2115 | 642.4084 | 642.4177 | -14.4 | 1 | 17 | 0.8 | 1Score > 41 indicates identityScore > 29 indicates homology |
KGAVIR | |
| 429.5511 | 1285.6315 | 1285.6336 | -1.68 | 0 | 17 | 0.22 | 1Score > 40 indicates identityScore > 23 indicates homology |
GTVILDMAHGQK + Deamidated (NQ); Oxidation (M) | |
| 414.9817 | 1655.8977 | 1655.9028 | -3.09 | 1 | 17 | 0.25 | 1Score > 38 indicates identityScore > 24 indicates homology |
MKNNIINLAQDLIR + Deamidated (NQ) | |
| 797.4386 | 2389.2940 | 2389.3104 | -6.87 | 1 | 17 | 0.26 | 1Score > 35 indicates identityScore > 24 indicates homology |
LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ) | |
| 654.8454 | 1307.6762 | 1307.6721 | 3.17 | 1 | 17 | 0.6 | 1Score > 41 indicates identityScore > 28 indicates homology |
LYELIDSNSVR | |
| 579.7751 | 1157.5356 | 1157.5499 | -12.3 | 1 | 17 | 0.38 | 1Score > 40 indicates identityScore > 26 indicates homology |
VKVHDMNNGK + Deamidated (NQ); Oxidation (M) | |
| 602.8408 | 1203.6670 | 1203.6499 | 14.2 | 1 | 17 | 0.58 | 1Score > 39 indicates identityScore > 27 indicates homology |
LQNQLFIAKE + Deamidated (NQ) | |
| 761.4004 | 2281.1794 | 2281.1372 | 18.5 | 1 | 17 | 0.18 | 1Score > 38 indicates identityScore > 22 indicates homology |
MTGALNNSGTTAIFINQLRDK + Deamidated (NQ); Oxidation (M) | |
| 791.9006 | 1581.7866 | 1581.7998 | -8.34 | 1 | 17 | 0.21 | 1Score > 40 indicates identityScore > 23 indicates homology |
HVDDILESLQGLSR + Deamidated (NQ) | |
| 375.2009 | 748.3872 | 748.3967 | -12.6 | 0 | 17 | 0.85 | 1Score > 42 indicates identityScore > 29 indicates homology |
LSLSSNK + Deamidated (NQ) | |
| 862.4816 | 2584.4230 | 2584.3999 | 8.91 | 1 | 17 | 1 | 1Score > 32 indicates identityScore > 30 indicates homology |
SKGSATALYSQLLDTTLQILYLGK + Deamidated (NQ) | |
| 472.7954 | 943.5762 | 943.5590 | 18.3 | 1 | 17 | 0.92 | 1Score > 36 indicates identityScore > 29 indicates homology |
TIIEDLLK | |
| 519.3007 | 1554.8803 | 1554.8617 | 11.9 | 0 | 17 | 0.97 | 1Score > 36 indicates identityScore > 30 indicates homology |
VTQLLSLLANVQQE | |
| 586.8558 | 1171.6970 | 1171.6812 | 13.5 | 1 | 17 | 0.95 | 1Score > 35 indicates identityScore > 29 indicates homology |
LTLKLQTLNE | |
| 433.7178 | 865.4210 | 865.4294 | -9.60 | 1 | 17 | 0.73 | 1Score > 42 indicates identityScore > 28 indicates homology |
ALGYSEAR | |
| 317.8359 | 950.4859 | 950.4862 | -0.30 | 1 | 17 | 0.92 | 1Score > 41 indicates identityScore > 29 indicates homology |
AEWFTIGK | |
| 671.3814 | 1340.7482 | 1340.7551 | -5.12 | 0 | 17 | 0.35 | 1Score > 38 indicates identityScore > 25 indicates homology |
LNPSLVISLSQGL + Deamidated (NQ) | |
| 332.8549 | 995.5429 | 995.5440 | -1.14 | 1 | 17 | 0.26 | 1Score > 37 indicates identityScore > 24 indicates homology |
WTYKTGLK | |
| 629.8695 | 1257.7244 | 1257.7081 | 13.0 | 0 | 17 | 0.7 | 1Score > 37 indicates identityScore > 28 indicates homology |
LTPFLGVLQDR | |
| 488.7505 | 1950.9729 | 1950.9660 | 3.53 | 1 | 17 | 0.16 | 1Score > 40 indicates identityScore > 22 indicates homology |
SNRHIYAQVIDDVHQR + Deamidated (NQ) | |
| 506.2783 | 1010.5420 | 1010.5396 | 2.38 | 0 | 17 | 0.34 | 1Score > 39 indicates identityScore > 25 indicates homology |
HDVQSAILK + Deamidated (NQ) | |
| 491.7537 | 981.4928 | 981.4879 | 5.01 | 1 | 17 | 0.8 | 1Score > 40 indicates identityScore > 29 indicates homology |
AHQQLQKE + Deamidated (NQ) | |
| 710.0475 | 2127.1207 | 2127.0960 | 11.6 | 1 | 17 | 0.58 | 1Score > 38 indicates identityScore > 27 indicates homology |
AADTAGDLAGLEIAGPGFINVR | |
| 332.8552 | 995.5438 | 995.5400 | 3.80 | 1 | 17 | 0.44 | 1Score > 37 indicates identityScore > 26 indicates homology |
EGQVTPLPR | |
| 336.2201 | 670.4256 | 670.4126 | 19.5 | 1 | 17 | 0.52 | 1Score > 40 indicates identityScore > 27 indicates homology |
RVTAPK | |
| 790.4517 | 1578.8888 | 1578.8729 | 10.1 | 1 | 17 | 0.5 | 1Score > 35 indicates identityScore > 27 indicates homology |
ELQANVVHLSLLSR + Deamidated (NQ) | |
| 537.0302 | 2144.0917 | 2144.1225 | -14.4 | 1 | 17 | 0.23 | 1Score > 39 indicates identityScore > 23 indicates homology |
ANGAIFTAQGKALNAVAAADIR + 2 Deamidated (NQ) | |
| 667.0072 | 1997.9998 | 1997.9656 | 17.1 | 0 | 17 | 0.32 | 1Score > 39 indicates identityScore > 24 indicates homology |
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M) | |
| 794.0991 | 2379.2755 | 2379.2885 | -5.49 | 0 | 17 | 0.1 | 1Score > 35 indicates identityScore > 20 indicates homology |
FGIGADGVLFLAKPLHTSLHMR | |
| 620.3410 | 1238.6674 | 1238.6805 | -10.5 | 1 | 17 | 0.54 | 1Score > 39 indicates identityScore > 27 indicates homology |
ALHQQICKIK + Deamidated (NQ) | |
| 820.9227 | 1639.8308 | 1639.8279 | 1.77 | 0 | 17 | 0.29 | 1Score > 40 indicates identityScore > 24 indicates homology |
WIAYAQSLTSKPMK + Deamidated (NQ); Oxidation (M) | |
| 301.1697 | 600.3248 | 600.3231 | 2.90 | 0 | 17 | 0.88 | 1Score > 39 indicates identityScore > 29 indicates homology |
EPSIR | |
| 365.2259 | 728.4372 | 728.4293 | 10.9 | 1 | 17 | 0.89 | 1Score > 41 indicates identityScore > 29 indicates homology |
LAANGKR | |
| 458.2297 | 1371.6673 | 1371.6526 | 10.7 | 1 | 17 | 0.32 | 1Score > 40 indicates identityScore > 24 indicates homology |
MMYKDVTQALR + Deamidated (NQ); Oxidation (M) | |
| 602.3541 | 601.3468 | 601.3435 | 5.54 | 1 | 17 | 0.7 | 1Score > 45 indicates identityScore > 28 indicates homology |
AEAIAK | |
| 351.6940 | 701.3734 | 701.3708 | 3.81 | 1 | 17 | 0.89 | 1Score > 45 indicates identityScore > 29 indicates homology |
NEAGALK | |
| 356.7228 | 711.4310 | 711.4391 | -11.4 | 1 | 17 | 0.25 | 1Score > 34 indicates identityScore > 23 indicates homology |
ARAGLPK | |
| 392.7703 | 783.5260 | 783.5331 | -8.94 | 1 | 17 | 0.38 | 1Score > 32 indicates identityScore > 25 indicates homology |
LGIVVKR | |
| 879.9954 | 1757.9762 | 1757.9563 | 11.3 | 1 | 17 | 0.26 | 1Score > 36 indicates identityScore > 23 indicates homology |
KLNSAGINILPQLTFE + Deamidated (NQ) | |
| 301.6985 | 601.3824 | 601.3911 | -14.4 | 1 | 17 | 0.092 | 1Score > 41 indicates identityScore > 19 indicates homology |
ISVKR | |
| 425.2283 | 848.4420 | 848.4392 | 3.35 | 0 | 17 | 0.92 | 1Score > 42 indicates identityScore > 29 indicates homology |
ALFDDLR | |
| 417.9074 | 1250.7004 | 1250.7095 | -7.30 | 1 | 17 | 0.58 | 1Score > 37 indicates identityScore > 27 indicates homology |
KAGADGITIHLR | |
| 794.1021 | 2379.2845 | 2379.2885 | -1.71 | 0 | 17 | 0.16 | 1Score > 35 indicates identityScore > 21 indicates homology |
FGIGADGVLFLAKPLHTSLHMR | |
| 748.3679 | 1494.7212 | 1494.7427 | -14.3 | 1 | 17 | 0.36 | 1Score > 41 indicates identityScore > 25 indicates homology |
LGSYSGPTSVSRQR + Deamidated (NQ) | |
| 445.5633 | 1333.6681 | 1333.6911 | -17.3 | 1 | 17 | 0.7 | 1Score > 42 indicates identityScore > 28 indicates homology |
MQISVESVNSIK | |
| 521.7992 | 1041.5838 | 1041.5818 | 1.93 | 0 | 17 | 0.76 | 1Score > 40 indicates identityScore > 28 indicates homology |
ITGQKPQIR + 2 Deamidated (NQ) | |
| 380.2552 | 758.4958 | 758.4902 | 7.48 | 0 | 17 | 0.28 | 1Score > 28 indicates identityScore > 24 indicates homology |
SVLSILK | |
| 891.4703 | 2671.3891 | 2671.4327 | -16.3 | 1 | 17 | 0.1 | 1Score > 36 indicates identityScore > 19 indicates homology |
GGSLVGSALQGRVMLVDDVITAGTAIR + Oxidation (M) | |
| 428.7315 | 855.4484 | 855.4450 | 4.00 | 1 | 17 | 0.55 | 1Score > 39 indicates identityScore > 27 indicates homology |
LDGVPAER | |
| 524.7764 | 1047.5382 | 1047.5461 | -7.54 | 1 | 17 | 0.42 | 1Score > 41 indicates identityScore > 25 indicates homology |
QSKQFTPGR | |
| 578.5979 | 1732.7719 | 1732.7951 | -13.4 | 1 | 17 | 0.1 | 1Score > 38 indicates identityScore > 19 indicates homology |
HNQSPRNTYQSAMAK + Deamidated (NQ) | |
| 651.3589 | 1300.7032 | 1300.7139 | -8.21 | 0 | 17 | 0.99 | 1Score > 41 indicates identityScore > 29 indicates homology |
WIASGLTDVLAR | |
| 396.8770 | 1187.6092 | 1187.5968 | 10.4 | 1 | 17 | 0.26 | 1Score > 42 indicates identityScore > 23 indicates homology |
KNGAADPVMTGK | |
| 751.4055 | 1500.7964 | 1500.8188 | -14.9 | 0 | 17 | 0.74 | 1Score > 40 indicates identityScore > 28 indicates homology |
IITQLIVDSFHSK + Deamidated (NQ) | |
| 558.3643 | 557.3570 | 557.3537 | 5.99 | 0 | 17 | 0.54 | 1Score > 40 indicates identityScore > 26 indicates homology |
VIAGAK | |
| 409.7180 | 817.4214 | 817.4181 | 4.05 | 0 | 17 | 0.74 | 1Score > 44 indicates identityScore > 28 indicates homology |
QDSLDIK | |
| 1017.5076 | 2033.0006 | 2032.9636 | 18.2 | 1 | 17 | 0.25 | 1Score > 40 indicates identityScore > 23 indicates homology |
RDANNIHSLTQFQAMLR + 3 Deamidated (NQ); Oxidation (M) | |
| 518.2975 | 1034.5804 | 1034.5648 | 15.1 | 0 | 17 | 0.51 | 1Score > 38 indicates identityScore > 26 indicates homology |
IFLNSVVDK + Deamidated (NQ) | |
| 854.4759 | 853.4686 | 853.4657 | 3.39 | 0 | 16 | 0.74 | 1Score > 38 indicates identityScore > 28 indicates homology |
APAAAPAASK | |
| 410.8683 | 1229.5831 | 1229.5598 | 18.9 | 1 | 16 | 0.33 | 1Score > 40 indicates identityScore > 24 indicates homology |
KMPIPAGSDGQE + Deamidated (NQ) | |
| 337.6969 | 673.3792 | 673.3759 | 5.02 | 1 | 16 | 0.5 | 1Score > 42 indicates identityScore > 26 indicates homology |
IESAVR | |
| 304.4163 | 1213.6361 | 1213.6163 | 16.3 | 1 | 16 | 0.66 | 1Score > 41 indicates identityScore > 27 indicates homology |
NNLGVRNAAQR + 2 Deamidated (NQ) | |
| 747.8839 | 1493.7532 | 1493.7435 | 6.50 | 1 | 16 | 0.64 | 1Score > 41 indicates identityScore > 27 indicates homology |
YIDNKITMAIPAE + Oxidation (M) | |
| 460.7484 | 1838.9645 | 1838.9924 | -15.2 | 0 | 16 | 0.33 | 1Score > 38 indicates identityScore > 24 indicates homology |
TSTIGLGMPTAPAAVLAPR + Oxidation (M) | |
| 747.8838 | 1493.7530 | 1493.7435 | 6.36 | 1 | 16 | 0.65 | 1Score > 41 indicates identityScore > 27 indicates homology |
YIDNKITMAIPAE + Oxidation (M) | |
| 388.2327 | 774.4508 | 774.4487 | 2.79 | 0 | 16 | 0.95 | 1Score > 39 indicates identityScore > 29 indicates homology |
LSNSIIK + Deamidated (NQ) | |
| 648.3787 | 1294.7428 | 1294.7609 | -13.9 | 1 | 16 | 0.44 | 1Score > 35 indicates identityScore > 25 indicates homology |
VVGLSTQLPPRK + Deamidated (NQ) | |
| 772.4281 | 1542.8416 | 1542.8617 | -13.0 | 1 | 16 | 0.78 | 1Score > 39 indicates identityScore > 28 indicates homology |
SILSDLEGTAIVAVR | |
| 850.1060 | 2547.2962 | 2547.2924 | 1.48 | 0 | 16 | 0.073 | 1Score > 38 indicates identityScore > 18 indicates homology |
MQLLSCIALTLALVANGAPTSSSTE | |
| 308.6877 | 615.3608 | 615.3592 | 2.74 | 0 | 16 | 0.72 | 1Score > 45 indicates identityScore > 27 indicates homology |
IDGLAK | |
| 490.9320 | 1469.7742 | 1469.7772 | -2.08 | 1 | 16 | 0.84 | 1Score > 40 indicates identityScore > 28 indicates homology |
MNAQPRSALALAAR + Deamidated (NQ) | |
| 923.4556 | 1844.8966 | 1844.8839 | 6.90 | 1 | 16 | 0.24 | 1Score > 40 indicates identityScore > 23 indicates homology |
SHVTLPQIRGMFNGDR + 2 Deamidated (NQ); Oxidation (M) | |
| 761.4528 | 760.4455 | 760.4443 | 1.62 | 0 | 16 | 0.86 | 1Score > 38 indicates identityScore > 28 indicates homology |
TTIASLR | |
| 348.1724 | 694.3302 | 694.3286 | 2.40 | 1 | 16 | 0.73 | 1Score > 41 indicates identityScore > 27 indicates homology |
IYEDR | |
| 470.2359 | 938.4572 | 938.4756 | -19.6 | 1 | 16 | 0.52 | 1Score > 40 indicates identityScore > 26 indicates homology |
RMNTFVR + Oxidation (M) | |
| 391.2049 | 1170.5929 | 1170.5768 | 13.7 | 1 | 16 | 0.42 | 1Score > 40 indicates identityScore > 25 indicates homology |
IAQALVENPSE + Deamidated (NQ) | |
| 752.4171 | 1502.8196 | 1502.8279 | -5.48 | 1 | 16 | 0.63 | 1Score > 38 indicates identityScore > 27 indicates homology |
AYEPLIRIICQK | |
| 547.3250 | 546.3177 | 546.3126 | 9.45 | 0 | 16 | 0.41 | 1Score > 38 indicates identityScore > 25 indicates homology |
ITGTR | |
| 388.2040 | 1161.5902 | 1161.5877 | 2.11 | 0 | 16 | 0.85 | 1Score > 42 indicates identityScore > 28 indicates homology |
TQSTAQILDGK + Deamidated (NQ) | |
| 440.7260 | 879.4374 | 879.4484 | -12.4 | 1 | 16 | 0.96 | 1Score > 42 indicates identityScore > 28 indicates homology |
KLCASSSK | |
| 499.8001 | 997.5856 | 997.5920 | -6.35 | 1 | 16 | 0.71 | 1Score > 38 indicates identityScore > 27 indicates homology |
KQNRPLLK + 2 Deamidated (NQ) | |
| 752.3882 | 1502.7618 | 1502.7689 | -4.67 | 1 | 16 | 0.8 | 1Score > 41 indicates identityScore > 28 indicates homology |
LTSQARGLSSQNLE | |
| 648.8705 | 1295.7264 | 1295.7197 | 5.20 | 1 | 16 | 0.91 | 1Score > 37 indicates identityScore > 28 indicates homology |
RLPQNGQLQLK + 2 Deamidated (NQ) | |
| 774.8888 | 1547.7630 | 1547.7831 | -13.0 | 1 | 16 | 0.27 | 1Score > 41 indicates identityScore > 23 indicates homology |
NVVEVTFNDLAAQK + Deamidated (NQ) | |
| 457.7747 | 913.5348 | 913.5233 | 12.7 | 1 | 16 | 0.42 | 1Score > 40 indicates identityScore > 25 indicates homology |
QNKTVVPK + Deamidated (NQ) | |
| 479.2747 | 1434.8023 | 1434.7830 | 13.4 | 1 | 16 | 0.43 | 1Score > 36 indicates identityScore > 25 indicates homology |
LNLIHQGLEQIR + 2 Deamidated (NQ) | |
| 407.7573 | 813.5000 | 813.4960 | 4.97 | 1 | 16 | 0.61 | 1Score > 39 indicates identityScore > 26 indicates homology |
LGAEVVVK | |
| 434.2941 | 866.5736 | 866.5701 | 4.04 | 0 | 16 | 0.13 | 1Score > 28 indicates identityScore > 20 indicates homology |
LGLKPLAR | |
| 566.2922 | 1130.5698 | 1130.5680 | 1.65 | 1 | 16 | 0.21 | 1Score > 41 indicates identityScore > 22 indicates homology |
NSVQEALNTR | |
| 448.6024 | 1342.7854 | 1342.7820 | 2.51 | 1 | 16 | 0.39 | 1Score > 35 indicates identityScore > 24 indicates homology |
TSLLRAVQQVVK + 2 Deamidated (NQ) | |
| 400.7496 | 799.4846 | 799.4916 | -8.67 | 1 | 16 | 0.9 | 1Score > 40 indicates identityScore > 28 indicates homology |
DVKVIAR | |
| 375.8659 | 1124.5759 | 1124.5536 | 19.8 | 1 | 16 | 0.62 | 1Score > 40 indicates identityScore > 27 indicates homology |
MVINSELFR + Deamidated (NQ); Oxidation (M) | |
| 805.3831 | 1608.7516 | 1608.7600 | -5.17 | 1 | 16 | 0.73 | 1Score > 40 indicates identityScore > 27 indicates homology |
KMGMNIINDDITGR + 2 Oxidation (M) |
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