MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2090 471.2875 1410.8407 1410.8194 15.1 1 17 0.56 +1Score > 33 indicates identity
Score > 27 indicates homology
DLKQQLLLLNGR + Deamidated (NQ)
2384 781.4021 1560.7896 1560.8082 -11.9 0 17 0.84 +1Score > 41 indicates identity
Score > 29 indicates homology
MIVFVNQRPVDSR + Deamidated (NQ)
1417 560.2787 1118.5428 1118.5356 6.47 1 17 0.98 +1Score > 42 indicates identity
Score > 30 indicates homology
QANQSEFAPK
1572 589.2988 1176.5830 1176.6060 -19.5 1 17 0.59 +1Score > 42 indicates identity
Score > 28 indicates homology
ALAKLDISAME + Oxidation (M)
1266 347.2237 1038.6493 1038.6477 1.49 1 17 0.98 +1Score > 33 indicates identity
Score > 30 indicates homology
LYIYKVLK
1900 660.3090 1318.6034 1318.6153 -9.02 0 17 0.094 +1Score > 40 indicates identity
Score > 20 indicates homology
TLDISDADWQR
2110 711.3491 1420.6836 1420.6834 0.18 1 17 0.77 +1Score > 41 indicates identity
Score > 29 indicates homology
INQADGAVDKFNK + 2 Deamidated (NQ)
404 322.2115 642.4084 642.4177 -14.4 1 17 0.8 +1Score > 41 indicates identity
Score > 29 indicates homology
KGAVIR
1829 429.5511 1285.6315 1285.6336 -1.68 0 17 0.22 +1Score > 40 indicates identity
Score > 23 indicates homology
GTVILDMAHGQK + Deamidated (NQ); Oxidation (M)
2608 414.9817 1655.8977 1655.9028 -3.09 1 17 0.25 +1Score > 38 indicates identity
Score > 24 indicates homology
MKNNIINLAQDLIR + Deamidated (NQ)
3777 797.4386 2389.2940 2389.3104 -6.87 1 17 0.26 +1Score > 35 indicates identity
Score > 24 indicates homology
LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ)
1875 654.8454 1307.6762 1307.6721 3.17 1 17 0.6 +1Score > 41 indicates identity
Score > 28 indicates homology
LYELIDSNSVR
1519 579.7751 1157.5356 1157.5499 -12.3 1 17 0.38 +1Score > 40 indicates identity
Score > 26 indicates homology
VKVHDMNNGK + Deamidated (NQ); Oxidation (M)
1640 602.8408 1203.6670 1203.6499 14.2 1 17 0.58 +1Score > 39 indicates identity
Score > 27 indicates homology
LQNQLFIAKE + Deamidated (NQ)
3628 761.4004 2281.1794 2281.1372 18.5 1 17 0.18 +1Score > 38 indicates identity
Score > 22 indicates homology
MTGALNNSGTTAIFINQLRDK + Deamidated (NQ); Oxidation (M)
2442 791.9006 1581.7866 1581.7998 -8.34 1 17 0.21 +1Score > 40 indicates identity
Score > 23 indicates homology
HVDDILESLQGLSR + Deamidated (NQ)
610 375.2009 748.3872 748.3967 -12.6 0 17 0.85 +1Score > 42 indicates identity
Score > 29 indicates homology
LSLSSNK + Deamidated (NQ)
3977 862.4816 2584.4230 2584.3999 8.91 1 17 1 +1Score > 32 indicates identity
Score > 30 indicates homology
SKGSATALYSQLLDTTLQILYLGK + Deamidated (NQ)
1029 472.7954 943.5762 943.5590 18.3 1 17 0.92 +1Score > 36 indicates identity
Score > 29 indicates homology
TIIEDLLK
2371 519.3007 1554.8803 1554.8617 11.9 0 17 0.97 +1Score > 36 indicates identity
Score > 30 indicates homology
VTQLLSLLANVQQE
1564 586.8558 1171.6970 1171.6812 13.5 1 17 0.95 +1Score > 35 indicates identity
Score > 29 indicates homology
LTLKLQTLNE
851 433.7178 865.4210 865.4294 -9.60 1 17 0.73 +1Score > 42 indicates identity
Score > 28 indicates homology
ALGYSEAR
1055 317.8359 950.4859 950.4862 -0.30 1 17 0.92 +1Score > 41 indicates identity
Score > 29 indicates homology
AEWFTIGK
1945 671.3814 1340.7482 1340.7551 -5.12 0 17 0.35 +1Score > 38 indicates identity
Score > 25 indicates homology
LNPSLVISLSQGL + Deamidated (NQ)
1158 332.8549 995.5429 995.5440 -1.14 1 17 0.26 +1Score > 37 indicates identity
Score > 24 indicates homology
WTYKTGLK
1771 629.8695 1257.7244 1257.7081 13.0 0 17 0.7 +1Score > 37 indicates identity
Score > 28 indicates homology
LTPFLGVLQDR
3090 488.7505 1950.9729 1950.9660 3.53 1 17 0.16 +1Score > 40 indicates identity
Score > 22 indicates homology
SNRHIYAQVIDDVHQR + Deamidated (NQ)
1198 506.2783 1010.5420 1010.5396 2.38 0 17 0.34 +1Score > 39 indicates identity
Score > 25 indicates homology
HDVQSAILK + Deamidated (NQ)
1127 491.7537 981.4928 981.4879 5.01 1 17 0.8 +1Score > 40 indicates identity
Score > 29 indicates homology
AHQQLQKE + Deamidated (NQ)
3360 710.0475 2127.1207 2127.0960 11.6 1 17 0.58 +1Score > 38 indicates identity
Score > 27 indicates homology
AADTAGDLAGLEIAGPGFINVR
1159 332.8552 995.5438 995.5400 3.80 1 17 0.44 +1Score > 37 indicates identity
Score > 26 indicates homology
EGQVTPLPR
447 336.2201 670.4256 670.4126 19.5 1 17 0.52 +1Score > 40 indicates identity
Score > 27 indicates homology
RVTAPK
2429 790.4517 1578.8888 1578.8729 10.1 1 17 0.5 +1Score > 35 indicates identity
Score > 27 indicates homology
ELQANVVHLSLLSR + Deamidated (NQ)
3388 537.0302 2144.0917 2144.1225 -14.4 1 17 0.23 +1Score > 39 indicates identity
Score > 23 indicates homology
ANGAIFTAQGKALNAVAAADIR + 2 Deamidated (NQ)
3156 667.0072 1997.9998 1997.9656 17.1 0 17 0.32 +1Score > 39 indicates identity
Score > 24 indicates homology
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
3761 794.0991 2379.2755 2379.2885 -5.49 0 17 0.1 +1Score > 35 indicates identity
Score > 20 indicates homology
FGIGADGVLFLAKPLHTSLHMR
1738 620.3410 1238.6674 1238.6805 -10.5 1 17 0.54 +1Score > 39 indicates identity
Score > 27 indicates homology
ALHQQICKIK + Deamidated (NQ)
2579 820.9227 1639.8308 1639.8279 1.77 0 17 0.29 +1Score > 40 indicates identity
Score > 24 indicates homology
WIAYAQSLTSKPMK + Deamidated (NQ); Oxidation (M)
314 301.1697 600.3248 600.3231 2.90 0 17 0.88 +1Score > 39 indicates identity
Score > 29 indicates homology
EPSIR
582 365.2259 728.4372 728.4293 10.9 1 17 0.89 +1Score > 41 indicates identity
Score > 29 indicates homology
LAANGKR
2002 458.2297 1371.6673 1371.6526 10.7 1 17 0.32 +1Score > 40 indicates identity
Score > 24 indicates homology
MMYKDVTQALR + Deamidated (NQ); Oxidation (M)
320 602.3541 601.3468 601.3435 5.54 1 17 0.7 +1Score > 45 indicates identity
Score > 28 indicates homology
AEAIAK
521 351.6940 701.3734 701.3708 3.81 1 17 0.89 +1Score > 45 indicates identity
Score > 29 indicates homology
NEAGALK
547 356.7228 711.4310 711.4391 -11.4 1 17 0.25 +1Score > 34 indicates identity
Score > 23 indicates homology
ARAGLPK
686 392.7703 783.5260 783.5331 -8.94 1 17 0.38 +1Score > 32 indicates identity
Score > 25 indicates homology
LGIVVKR
2788 879.9954 1757.9762 1757.9563 11.3 1 17 0.26 +1Score > 36 indicates identity
Score > 23 indicates homology
KLNSAGINILPQLTFE + Deamidated (NQ)
327 301.6985 601.3824 601.3911 -14.4 1 17 0.092 +1Score > 41 indicates identity
Score > 19 indicates homology
ISVKR
810 425.2283 848.4420 848.4392 3.35 0 17 0.92 +1Score > 42 indicates identity
Score > 29 indicates homology
ALFDDLR
1757 417.9074 1250.7004 1250.7095 -7.30 1 17 0.58 +1Score > 37 indicates identity
Score > 27 indicates homology
KAGADGITIHLR
3764 794.1021 2379.2845 2379.2885 -1.71 0 17 0.16 +1Score > 35 indicates identity
Score > 21 indicates homology
FGIGADGVLFLAKPLHTSLHMR
2241 748.3679 1494.7212 1494.7427 -14.3 1 17 0.36 +1Score > 41 indicates identity
Score > 25 indicates homology
LGSYSGPTSVSRQR + Deamidated (NQ)
1932 445.5633 1333.6681 1333.6911 -17.3 1 17 0.7 +1Score > 42 indicates identity
Score > 28 indicates homology
MQISVESVNSIK
1271 521.7992 1041.5838 1041.5818 1.93 0 17 0.76 +1Score > 40 indicates identity
Score > 28 indicates homology
ITGQKPQIR + 2 Deamidated (NQ)
631 380.2552 758.4958 758.4902 7.48 0 17 0.28 +1Score > 28 indicates identity
Score > 24 indicates homology
SVLSILK
4029 891.4703 2671.3891 2671.4327 -16.3 1 17 0.1 +1Score > 36 indicates identity
Score > 19 indicates homology
GGSLVGSALQGRVMLVDDVITAGTAIR + Oxidation (M)
830 428.7315 855.4484 855.4450 4.00 1 17 0.55 +1Score > 39 indicates identity
Score > 27 indicates homology
LDGVPAER
1284 524.7764 1047.5382 1047.5461 -7.54 1 17 0.42 +1Score > 41 indicates identity
Score > 25 indicates homology
QSKQFTPGR
2762 578.5979 1732.7719 1732.7951 -13.4 1 17 0.1 +1Score > 38 indicates identity
Score > 19 indicates homology
HNQSPRNTYQSAMAK + Deamidated (NQ)
1860 651.3589 1300.7032 1300.7139 -8.21 0 17 0.99 +1Score > 41 indicates identity
Score > 29 indicates homology
WIASGLTDVLAR
1597 396.8770 1187.6092 1187.5968 10.4 1 17 0.26 +1Score > 42 indicates identity
Score > 23 indicates homology
KNGAADPVMTGK
2257 751.4055 1500.7964 1500.8188 -14.9 0 17 0.74 +1Score > 40 indicates identity
Score > 28 indicates homology
IITQLIVDSFHSK + Deamidated (NQ)
282 558.3643 557.3570 557.3537 5.99 0 17 0.54 +1Score > 40 indicates identity
Score > 26 indicates homology
VIAGAK
744 409.7180 817.4214 817.4181 4.05 0 17 0.74 +1Score > 44 indicates identity
Score > 28 indicates homology
QDSLDIK
3215 1017.5076 2033.0006 2032.9636 18.2 1 17 0.25 +1Score > 40 indicates identity
Score > 23 indicates homology
RDANNIHSLTQFQAMLR + 3 Deamidated (NQ); Oxidation (M)
1254 518.2975 1034.5804 1034.5648 15.1 0 17 0.51 +1Score > 38 indicates identity
Score > 26 indicates homology
IFLNSVVDK + Deamidated (NQ)
822 854.4759 853.4686 853.4657 3.39 0 16 0.74 +1Score > 38 indicates identity
Score > 28 indicates homology
APAAAPAASK
1706 410.8683 1229.5831 1229.5598 18.9 1 16 0.33 +1Score > 40 indicates identity
Score > 24 indicates homology
KMPIPAGSDGQE + Deamidated (NQ)
464 337.6969 673.3792 673.3759 5.02 1 16 0.5 +1Score > 42 indicates identity
Score > 26 indicates homology
IESAVR
1656 304.4163 1213.6361 1213.6163 16.3 1 16 0.66 +1Score > 41 indicates identity
Score > 27 indicates homology
NNLGVRNAAQR + 2 Deamidated (NQ)
2238 747.8839 1493.7532 1493.7435 6.50 1 16 0.64 +1Score > 41 indicates identity
Score > 27 indicates homology
YIDNKITMAIPAE + Oxidation (M)
2936 460.7484 1838.9645 1838.9924 -15.2 0 16 0.33 +1Score > 38 indicates identity
Score > 24 indicates homology
TSTIGLGMPTAPAAVLAPR + Oxidation (M)
2236 747.8838 1493.7530 1493.7435 6.36 1 16 0.65 +1Score > 41 indicates identity
Score > 27 indicates homology
YIDNKITMAIPAE + Oxidation (M)
669 388.2327 774.4508 774.4487 2.79 0 16 0.95 +1Score > 39 indicates identity
Score > 29 indicates homology
LSNSIIK + Deamidated (NQ)
1847 648.3787 1294.7428 1294.7609 -13.9 1 16 0.44 +1Score > 35 indicates identity
Score > 25 indicates homology
VVGLSTQLPPRK + Deamidated (NQ)
2349 772.4281 1542.8416 1542.8617 -13.0 1 16 0.78 +1Score > 39 indicates identity
Score > 28 indicates homology
SILSDLEGTAIVAVR
3939 850.1060 2547.2962 2547.2924 1.48 0 16 0.073 +1Score > 38 indicates identity
Score > 18 indicates homology
MQLLSCIALTLALVANGAPTSSSTE
355 308.6877 615.3608 615.3592 2.74 0 16 0.72 +1Score > 45 indicates identity
Score > 27 indicates homology
IDGLAK
2192 490.9320 1469.7742 1469.7772 -2.08 1 16 0.84 +1Score > 40 indicates identity
Score > 28 indicates homology
MNAQPRSALALAAR + Deamidated (NQ)
2940 923.4556 1844.8966 1844.8839 6.90 1 16 0.24 +1Score > 40 indicates identity
Score > 23 indicates homology
SHVTLPQIRGMFNGDR + 2 Deamidated (NQ); Oxidation (M)
639 761.4528 760.4455 760.4443 1.62 0 16 0.86 +1Score > 38 indicates identity
Score > 28 indicates homology
TTIASLR
505 348.1724 694.3302 694.3286 2.40 1 16 0.73 +1Score > 41 indicates identity
Score > 27 indicates homology
IYEDR
1009 470.2359 938.4572 938.4756 -19.6 1 16 0.52 +1Score > 40 indicates identity
Score > 26 indicates homology
RMNTFVR + Oxidation (M)
1553 391.2049 1170.5929 1170.5768 13.7 1 16 0.42 +1Score > 40 indicates identity
Score > 25 indicates homology
IAQALVENPSE + Deamidated (NQ)
2267 752.4171 1502.8196 1502.8279 -5.48 1 16 0.63 +1Score > 38 indicates identity
Score > 27 indicates homology
AYEPLIRIICQK
273 547.3250 546.3177 546.3126 9.45 0 16 0.41 +1Score > 38 indicates identity
Score > 25 indicates homology
ITGTR
1533 388.2040 1161.5902 1161.5877 2.11 0 16 0.85 +1Score > 42 indicates identity
Score > 28 indicates homology
TQSTAQILDGK + Deamidated (NQ)
882 440.7260 879.4374 879.4484 -12.4 1 16 0.96 +1Score > 42 indicates identity
Score > 28 indicates homology
KLCASSSK
1168 499.8001 997.5856 997.5920 -6.35 1 16 0.71 +1Score > 38 indicates identity
Score > 27 indicates homology
KQNRPLLK + 2 Deamidated (NQ)
2260 752.3882 1502.7618 1502.7689 -4.67 1 16 0.8 +1Score > 41 indicates identity
Score > 28 indicates homology
LTSQARGLSSQNLE
1849 648.8705 1295.7264 1295.7197 5.20 1 16 0.91 +1Score > 37 indicates identity
Score > 28 indicates homology
RLPQNGQLQLK + 2 Deamidated (NQ)
2356 774.8888 1547.7630 1547.7831 -13.0 1 16 0.27 +1Score > 41 indicates identity
Score > 23 indicates homology
NVVEVTFNDLAAQK + Deamidated (NQ)
946 457.7747 913.5348 913.5233 12.7 1 16 0.42 +1Score > 40 indicates identity
Score > 25 indicates homology
QNKTVVPK + Deamidated (NQ)
2139 479.2747 1434.8023 1434.7830 13.4 1 16 0.43 +1Score > 36 indicates identity
Score > 25 indicates homology
LNLIHQGLEQIR + 2 Deamidated (NQ)
740 407.7573 813.5000 813.4960 4.97 1 16 0.61 +1Score > 39 indicates identity
Score > 26 indicates homology
LGAEVVVK
859 434.2941 866.5736 866.5701 4.04 0 16 0.13 +1Score > 28 indicates identity
Score > 20 indicates homology
LGLKPLAR
1456 566.2922 1130.5698 1130.5680 1.65 1 16 0.21 +1Score > 41 indicates identity
Score > 22 indicates homology
NSVQEALNTR
1946 448.6024 1342.7854 1342.7820 2.51 1 16 0.39 +1Score > 35 indicates identity
Score > 24 indicates homology
TSLLRAVQQVVK + 2 Deamidated (NQ)
714 400.7496 799.4846 799.4916 -8.67 1 16 0.9 +1Score > 40 indicates identity
Score > 28 indicates homology
DVKVIAR
1435 375.8659 1124.5759 1124.5536 19.8 1 16 0.62 +1Score > 40 indicates identity
Score > 27 indicates homology
MVINSELFR + Deamidated (NQ); Oxidation (M)
2496 805.3831 1608.7516 1608.7600 -5.17 1 16 0.73 +1Score > 40 indicates identity
Score > 27 indicates homology
KMGMNIINDDITGR + 2 Oxidation (M)
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