MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
590 734.3696 733.3623 733.3606 2.32 1 19 0.97 +1Score > 42 indicates identity
Score > 31 indicates homology		 DTTLER
3200 675.0381 2022.0925 2022.0606 15.8 1 19 0.15 +1Score > 36 indicates identity
Score > 23 indicates homology		 GLHARVAQAATTNGSIDLAR + Deamidated (NQ)
487 344.2250 686.4354 686.4327 4.06 0 19 0.83 +1Score > 40 indicates identity
Score > 30 indicates homology		 SVGAILK
798 422.7454 843.4762 843.4814 -6.13 0 19 0.66 +1Score > 43 indicates identity
Score > 29 indicates homology		 TAISPTVR
920 449.7429 897.4712 897.4668 4.93 0 19 0.6 +1Score > 39 indicates identity
Score > 29 indicates homology		 QQLTGHSK
823 427.7422 853.4698 853.4657 4.83 0 19 0.094 +1Score > 37 indicates identity
Score > 21 indicates homology		 APAAAPAASK
958 459.7618 917.5090 917.5004 9.41 1 19 0.28 +1Score > 40 indicates identity
Score > 26 indicates homology		 VLAESMIR
2165 485.9585 1454.8537 1454.8318 15.1 1 19 0.53 +1Score > 33 indicates identity
Score > 28 indicates homology		 TAVVVGAGRSGLAAAR
1020 314.5249 940.5529 940.5593 -6.85 1 19 0.34 +1Score > 39 indicates identity
Score > 26 indicates homology		 KVQLIDPK + Deamidated (NQ)
2299 758.3727 1514.7308 1514.7293 1.01 1 19 0.79 +1Score > 40 indicates identity
Score > 30 indicates homology		 VFSVFALFENDVE
1026 943.4961 942.4888 942.5022 -14.2 0 19 0.93 +1Score > 40 indicates identity
Score > 31 indicates homology		 NAGVLDLIE
3094 652.3802 1954.1188 1954.1188 -0.0092 0 19 0.85 +1Score > 31 indicates identity
Score > 30 indicates homology		 YLMPLLFIFLIGMVIR + Oxidation (M)
956 458.7717 915.5288 915.5389 -11.0 0 19 0.5 +1Score > 40 indicates identity
Score > 28 indicates homology		 VNLLTLSR + Deamidated (NQ)
1641 602.8427 1203.6708 1203.6645 5.26 1 19 0.2 +1Score > 39 indicates identity
Score > 24 indicates homology		 IISMQKGGNLK + Oxidation (M)
1365 545.2842 1088.5538 1088.5713 -16.1 0 19 0.81 +1Score > 42 indicates identity
Score > 30 indicates homology		 QNQIVLTSGK + 2 Deamidated (NQ)
2379 520.6163 1558.8271 1558.8202 4.39 1 19 0.85 +1Score > 40 indicates identity
Score > 30 indicates homology		 SNVGTLGQILGETIR + 2 Deamidated (NQ)
1073 479.7364 957.4582 957.4516 6.98 1 19 0.3 +1Score > 40 indicates identity
Score > 26 indicates homology		 TPNGEDAVR
1535 581.8035 1161.5924 1161.5778 12.6 0 19 0.81 +1Score > 42 indicates identity
Score > 30 indicates homology		 QPAFTVQASGR + Deamidated (NQ)
2343 772.4083 1542.8020 1542.8140 -7.77 0 19 0.16 +1Score > 40 indicates identity
Score > 23 indicates homology		 LAIQNSQIASAIALE + 2 Deamidated (NQ)
1611 597.3414 1192.6682 1192.6676 0.52 1 18 0.67 +1Score > 38 indicates identity
Score > 29 indicates homology		 GALEVRPPQAR
3061 965.5303 1929.0460 1929.0149 16.2 1 18 0.14 +1Score > 36 indicates identity
Score > 23 indicates homology		 FLSAPWVGLLEDGAWLR
4256 976.5308 2926.5706 2926.6254 -18.7 1 18 1 +1Score > 33 indicates identity
Score > 31 indicates homology		 ARIAGVNIPNHQHTVIGLTAIFGIGTTR
474 340.6807 679.3468 679.3442 3.91 0 18 0.52 +1Score > 40 indicates identity
Score > 28 indicates homology		 FPNFR
1210 1015.6136 1014.6063 1014.5896 16.5 0 18 0.44 +1Score > 37 indicates identity
Score > 27 indicates homology		 SVIMALIPR + Oxidation (M)
2713 855.4555 1708.8964 1708.8672 17.1 1 18 0.66 +1Score > 39 indicates identity
Score > 29 indicates homology		 QLAGDIALELQAYFR + 2 Deamidated (NQ)
1408 557.8307 1113.6468 1113.6393 6.75 0 18 0.84 +1Score > 39 indicates identity
Score > 30 indicates homology		 IANVLLASNAK + Deamidated (NQ)
1249 517.7531 1033.4916 1033.4928 -1.08 0 18 0.66 +1Score > 42 indicates identity
Score > 29 indicates homology		 TLSDSGLNVE
2647 836.4313 1670.8480 1670.8250 13.8 1 18 0.27 +1Score > 40 indicates identity
Score > 25 indicates homology		 QNGLTEVNGPVLVLSE + 3 Deamidated (NQ)
3333 706.0630 2115.1672 2115.1398 12.9 1 18 0.25 +1Score > 34 indicates identity
Score > 25 indicates homology		 MPNTNPLFIDALASVVLKR + Deamidated (NQ); Oxidation (M)
1948 672.8500 1343.6854 1343.6867 -0.93 1 18 0.61 +1Score > 41 indicates identity
Score > 29 indicates homology		 AIKIMQNEPQR + Deamidated (NQ); Oxidation (M)
2487 804.4298 1606.8450 1606.8467 -1.03 1 18 0.21 +1Score > 39 indicates identity
Score > 24 indicates homology		 VNYKIANTGNSLWK
1779 633.8354 1265.6562 1265.6768 -16.3 1 18 0.55 +1Score > 40 indicates identity
Score > 28 indicates homology		 VDYVRVYQPK
1820 427.5945 1279.7617 1279.7387 17.9 0 18 0.97 +1Score > 33 indicates identity
Score > 31 indicates homology		 AVSILNDLPPLK + Deamidated (NQ)
495 345.2027 688.3908 688.3908 0.058 0 18 0.18 +1Score > 41 indicates identity
Score > 23 indicates homology		 VAFPAGK
534 352.2222 702.4298 702.4276 3.23 0 18 0.85 +1Score > 38 indicates identity
Score > 30 indicates homology		 ITTAGIK
2911 456.7454 1822.9525 1822.9438 4.75 0 18 0.95 +1Score > 39 indicates identity
Score > 31 indicates homology		 NKPHLNVGTIGHVDHGK + Deamidated (NQ)
1788 635.3824 1268.7502 1268.7704 -15.9 0 18 0.89 +1Score > 35 indicates identity
Score > 30 indicates homology		 LIVIDLLSVQR + Deamidated (NQ)
1304 531.2967 1060.5788 1060.5699 8.45 1 18 0.31 +1Score > 41 indicates identity
Score > 26 indicates homology		 KINAVVNMR + Deamidated (NQ); Oxidation (M)
778 418.2626 834.5106 834.4963 17.2 0 18 0.99 +1Score > 33 indicates identity
Score > 31 indicates homology		 VPIHIQK + Deamidated (NQ)
1991 456.5703 1366.6891 1366.6803 6.45 1 18 0.5 +1Score > 40 indicates identity
Score > 28 indicates homology		 TSGFEMVQGLLGK + Deamidated (NQ)
2582 548.9666 1643.8780 1643.8552 13.8 1 18 0.17 +1Score > 39 indicates identity
Score > 23 indicates homology		 ADLEIQQLMLDALR + Oxidation (M)
1282 524.2654 1046.5162 1046.5033 12.4 0 18 0.77 +1Score > 42 indicates identity
Score > 29 indicates homology		 DNAPLQFDK
1345 361.8501 1082.5285 1082.5278 0.65 0 18 0.82 +1Score > 40 indicates identity
Score > 30 indicates homology		 SLSSSTCITK
2957 619.6648 1855.9726 1855.9891 -8.91 0 18 0.44 +1Score > 39 indicates identity
Score > 27 indicates homology		 VLTSDSNIVPVDISGIAR + Deamidated (NQ)
1161 332.8553 995.5441 995.5400 4.10 1 18 0.37 +1Score > 37 indicates identity
Score > 26 indicates homology		 EGQVTPLPR
2348 515.2875 1542.8407 1542.8617 -13.6 0 18 0.68 +1Score > 39 indicates identity
Score > 29 indicates homology		 SGATLNQLLVQSAIK + Deamidated (NQ)
2590 825.9312 1649.8478 1649.8624 -8.85 0 18 0.18 +1Score > 40 indicates identity
Score > 23 indicates homology		 ITTVQAAIDFIQSSR + Deamidated (NQ)
1261 519.2740 1036.5334 1036.5488 -14.8 1 18 0.15 +1Score > 40 indicates identity
Score > 22 indicates homology		 LAAAFKMNR + Oxidation (M)
2958 619.6658 1855.9756 1855.9891 -7.29 0 18 0.19 +1Score > 39 indicates identity
Score > 23 indicates homology		 VLTSDSNIVPVDISGIAR + Deamidated (NQ)
1294 527.8334 1053.6522 1053.6434 8.42 0 18 0.13 +1Score > 33 indicates identity
Score > 22 indicates homology		 VAEPLITLAK
2837 893.9833 1785.9520 1785.9585 -3.60 1 18 0.36 +1Score > 39 indicates identity
Score > 26 indicates homology		 RIQGGAADTVLLGATGTGK + Deamidated (NQ)
2935 920.4604 1838.9062 1838.8985 4.21 0 18 0.71 +1Score > 40 indicates identity
Score > 29 indicates homology		 QPSSFNGIVGMKPTYGR + Deamidated (NQ)
993 465.2774 928.5402 928.5342 6.55 1 18 0.53 +1Score > 41 indicates identity
Score > 28 indicates homology		 VLLEAGATR
2041 695.8879 1389.7612 1389.7616 -0.24 1 18 0.51 +1Score > 39 indicates identity
Score > 28 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
2146 481.6186 1441.8340 1441.8252 6.06 1 18 0.71 +1Score > 35 indicates identity
Score > 29 indicates homology		 VAIDSAISAQIKAR
2043 464.2614 1389.7624 1389.7616 0.57 1 18 0.27 +1Score > 39 indicates identity
Score > 25 indicates homology		 AQAKNLTFQQLK + Deamidated (NQ)
3553 745.4362 2233.2868 2233.2583 12.8 1 18 0.65 +1Score > 29 indicates identity SLTRFGFTLIIGGAIGNLIDR
713 400.7134 799.4122 799.4076 5.86 1 18 0.28 +1Score > 39 indicates identity
Score > 25 indicates homology		 KAGADPIE
716 401.2162 800.4178 800.4141 4.73 0 18 0.82 +1Score > 42 indicates identity
Score > 30 indicates homology		 VASGTNPR
2224 743.3763 1484.7380 1484.7260 8.15 0 18 0.56 +1Score > 40 indicates identity
Score > 28 indicates homology		 LWDDGIIDPANTR
2922 610.3136 1827.9190 1827.9407 -11.9 1 18 0.52 +1Score > 40 indicates identity
Score > 28 indicates homology		 FKNIIASPLSAGFNYGK + 2 Deamidated (NQ)
3140 663.6542 1987.9408 1987.9067 17.2 1 18 0.51 +1Score > 39 indicates identity
Score > 27 indicates homology		 DWMVMNMTQNRVFLR + 3 Oxidation (M)
346 307.7094 613.4042 613.4024 3.08 1 18 0.49 +1Score > 39 indicates identity
Score > 27 indicates homology		 AVRIR
883 440.7261 879.4376 879.4372 0.55 1 18 0.97 +1Score > 42 indicates identity
Score > 30 indicates homology		 GEMVSTLK + Oxidation (M)
230 488.2853 487.2780 487.2754 5.31 0 18 0.79 +1Score > 47 indicates identity
Score > 29 indicates homology		 AGNVK
943 456.7634 911.5122 911.5116 0.66 1 18 0.8 +1Score > 38 indicates identity
Score > 29 indicates homology		 KIVDYFK
1621 399.8961 1196.6665 1196.6448 18.1 1 18 0.99 +1Score > 38 indicates identity
Score > 30 indicates homology		 MQLRHINIR + Deamidated (NQ); Oxidation (M)
2373 519.3013 1554.8821 1554.8558 16.9 1 18 0.43 +1Score > 36 indicates identity
Score > 27 indicates homology		 RFPVALALSAPVWE
1279 523.2689 1044.5232 1044.5200 3.15 1 18 0.77 +1Score > 41 indicates identity
Score > 29 indicates homology		 GEQASNIIGR + Deamidated (NQ)
1609 398.2013 1191.5821 1191.5884 -5.32 0 18 0.94 +1Score > 41 indicates identity
Score > 30 indicates homology		 AVSSGPGVFSQR + Deamidated (NQ)
1644 604.7822 1207.5498 1207.5576 -6.46 1 18 0.95 +1Score > 41 indicates identity
Score > 30 indicates homology		 MANAANMIKVE + Deamidated (NQ); Oxidation (M)
807 424.7001 847.3856 847.3937 -9.46 1 18 0.3 +1Score > 41 indicates identity
Score > 25 indicates homology		 DDTWRR
3721 782.3581 2344.0525 2344.0729 -8.71 0 18 0.16 +1Score > 37 indicates identity
Score > 22 indicates homology		 GCTFHGLSLNVDMDLEPFHR
1440 564.8072 1127.5998 1127.5896 9.06 1 18 0.16 +1Score > 41 indicates identity
Score > 22 indicates homology		 LVEIQMPAVE
1741 414.2373 1239.6901 1239.7122 -17.8 1 18 0.66 +1Score > 38 indicates identity
Score > 29 indicates homology		 VGRCLGAPIAVK
1642 603.2945 1204.5744 1204.5684 5.05 1 18 0.81 +1Score > 41 indicates identity
Score > 29 indicates homology		 RQQSTVNNGAK + 3 Deamidated (NQ)
2347 772.4276 1542.8406 1542.8439 -2.12 0 18 0.45 +1Score > 39 indicates identity
Score > 27 indicates homology		 NMVVSNILLNNIAK + Deamidated (NQ)
2776 871.9976 1741.9806 1741.9475 19.0 0 18 0.28 +1Score > 35 indicates identity
Score > 25 indicates homology		 QLVQQGLGIFGGAIADR
2161 484.5874 1450.7404 1450.7589 -12.7 1 18 0.078 +1Score > 41 indicates identity
Score > 19 indicates homology		 LSTMLTTNAILKE + Deamidated (NQ); Oxidation (M)
3192 1010.5089 2019.0032 2019.0056 -1.19 1 18 0.65 +1Score > 40 indicates identity
Score > 28 indicates homology		 LESIVMNWLGQMLNLPK + 2 Deamidated (NQ); 2 Oxidation (M)
1504 385.2261 1152.6565 1152.6363 17.5 0 18 0.14 +1Score > 36 indicates identity
Score > 22 indicates homology		 TLRPAGAPTNR
3472 730.3722 2188.0948 2188.1198 -11.4 1 18 0.17 +1Score > 39 indicates identity
Score > 22 indicates homology		 INAAHGFSLIQVDNTKVTMK + 2 Deamidated (NQ)
1616 598.3884 1194.7622 1194.7448 14.6 0 18 0.12 +1Score > 27 indicates identity
Score > 21 indicates homology		 IVVALGGNALLR
2269 502.5968 1504.7686 1504.7634 3.45 1 18 0.64 +1Score > 41 indicates identity
Score > 28 indicates homology		 NTAQVFARDLVNR + 2 Deamidated (NQ)
254 516.3047 515.2974 515.2955 3.76 0 18 0.37 +1Score > 47 indicates identity
Score > 26 indicates homology		 ALAQL + Deamidated (NQ)
635 381.2014 760.3882 760.3902 -2.50 0 18 0.36 +1Score > 43 indicates identity
Score > 26 indicates homology		 KPMDVR + Oxidation (M)
1816 640.8503 1279.6860 1279.6996 -10.6 1 18 0.79 +1Score > 39 indicates identity
Score > 29 indicates homology		 NLSELRPAPGAR
857 434.2578 866.5010 866.4974 4.24 1 18 0.95 +1Score > 36 indicates identity
Score > 30 indicates homology		 HEILISR
341 307.1816 612.3486 612.3595 -17.7 1 18 0.28 +1Score > 38 indicates identity
Score > 25 indicates homology		 IEVPR
1217 508.7694 1015.5242 1015.5298 -5.48 0 18 0.32 +1Score > 42 indicates identity
Score > 25 indicates homology		 LGLSADDVAR
2039 695.8796 1389.7446 1389.7252 14.0 0 18 0.83 +1Score > 40 indicates identity
Score > 29 indicates homology		 QFLQQLSQLQR + 2 Deamidated (NQ)
1021 314.8370 941.4892 941.4930 -4.09 1 18 0.37 +1Score > 40 indicates identity
Score > 26 indicates homology		 SNPEVVAAR
753 412.7362 823.4578 823.4552 3.23 1 18 0.74 +1Score > 38 indicates identity
Score > 29 indicates homology		 SKTAYVR
2899 908.4629 1814.9112 1814.8911 11.1 1 18 0.066 +1Score > 40 indicates identity
Score > 18 indicates homology		 NNRFIGQAAIGGEPQDK + Deamidated (NQ)
1036 316.4922 946.4548 946.4469 8.37 0 18 0.95 +1Score > 42 indicates identity
Score > 30 indicates homology		 SVGGGGGGSGGAK
430 330.2091 658.4036 658.4126 -13.6 1 17 0.69 +1Score > 39 indicates identity
Score > 28 indicates homology		 IGSKVR
1490 571.8630 1141.7114 1141.7070 3.85 0 17 0.75 +1Score > 30 indicates identity
Score > 29 indicates homology		 ISIQVLQTLK
1569 587.7797 1173.5448 1173.5553 -8.93 1 17 0.92 +1Score > 40 indicates identity
Score > 30 indicates homology		 IQGWENLLAE + 2 Deamidated (NQ)
2916 609.3001 1824.8785 1824.8927 -7.81 1 17 0.46 +1Score > 40 indicates identity
Score > 27 indicates homology		 MLQLNSFAEISTLADR + Deamidated (NQ); Oxidation (M)
338 305.6886 609.3626 609.3598 4.59 0 17 0.18 +1Score > 34 indicates identity
Score > 23 indicates homology		 AGVHVK
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