MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
214 473.2853 472.2780 472.2758 4.77 0 20 0.56 +1Score > 43 indicates identity
Score > 30 indicates homology		 IGGAR
1296 529.2866 1056.5586 1056.5563 2.20 1 20 0.91 +1Score > 40 indicates identity
Score > 32 indicates homology		 IALEQANAAR + Deamidated (NQ)
624 380.2228 758.4310 758.4174 18.0 0 20 0.95 +1Score > 44 indicates identity
Score > 33 indicates homology		 VNVDAIK + Deamidated (NQ)
1187 504.7437 1007.4728 1007.4924 -19.4 1 20 0.74 +1Score > 42 indicates identity
Score > 31 indicates homology		 GASLADFAEK
435 333.6834 665.3522 665.3497 3.90 0 20 0.93 +1Score > 39 indicates identity
Score > 32 indicates homology		 IGYSAR
1453 566.2876 1130.5606 1130.5642 -3.11 0 20 0.6 +1Score > 42 indicates identity
Score > 31 indicates homology		 VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
904 447.7387 893.4628 893.4528 11.2 1 20 0.86 +1Score > 41 indicates identity
Score > 32 indicates homology		 TGVEMTLK + Oxidation (M)
502 347.6814 693.3482 693.3446 5.30 1 20 0.38 +1Score > 41 indicates identity
Score > 29 indicates homology		 EGAYVR
2857 599.3322 1794.9748 1794.9727 1.17 0 20 0.75 +1Score > 37 indicates identity
Score > 31 indicates homology		 IQNIVASANLGKPLNLE + 2 Deamidated (NQ)
2441 528.2693 1581.7861 1581.8038 -11.2 1 20 0.13 +1Score > 40 indicates identity
Score > 24 indicates homology		 AGPLPPLNQEAAFQK + 2 Deamidated (NQ)
1006 468.7626 935.5106 935.5076 3.23 0 20 0.83 +1Score > 40 indicates identity
Score > 32 indicates homology		 YLDGVTIR
1252 345.8509 1034.5309 1034.5145 15.8 1 20 0.87 +1Score > 42 indicates identity
Score > 32 indicates homology		 GNEFGVVTGR
334 305.1735 608.3324 608.3282 6.98 0 20 0.91 +1Score > 38 indicates identity
Score > 32 indicates homology		 YGTIR
1811 639.8436 1277.6726 1277.6615 8.70 0 20 0.34 +1Score > 41 indicates identity
Score > 28 indicates homology		 NGNQLIYNLTK + Deamidated (NQ)
2228 496.5935 1486.7587 1486.7793 -13.9 1 20 0.43 +1Score > 41 indicates identity
Score > 29 indicates homology		 LAQWFDAARRPR + Deamidated (NQ)
290 569.3065 568.2992 568.2969 4.08 0 20 0.17 +1Score > 38 indicates identity
Score > 25 indicates homology		 DVHAK
1361 544.3112 1086.6078 1086.6073 0.48 0 20 0.37 +1Score > 40 indicates identity
Score > 28 indicates homology		 VPIQIQNFK + Deamidated (NQ)
1833 645.3402 1288.6658 1288.6445 16.6 1 20 0.81 +1Score > 42 indicates identity
Score > 32 indicates homology		 QQSDILEMVAR
1919 663.4163 1324.8180 1324.8078 7.72 1 20 0.49 +1Score > 29 indicates identity NVVVVAANGKILK + Deamidated (NQ)
971 461.7370 921.4594 921.4630 -3.82 0 20 0.28 +1Score > 42 indicates identity
Score > 27 indicates homology		 IWQIMSK + Deamidated (NQ); Oxidation (M)
1315 356.8587 1067.5543 1067.5400 13.4 0 20 0.58 +1Score > 39 indicates identity
Score > 30 indicates homology		 TIVGNDFFR
910 447.7396 893.4646 893.4607 4.46 1 20 0.68 +1Score > 41 indicates identity
Score > 31 indicates homology		 LSGELYGR
2049 466.2613 1395.7621 1395.7650 -2.06 1 20 0.16 +1Score > 38 indicates identity
Score > 25 indicates homology		 LDGYDIISLIKF
257 517.3021 516.2948 516.2907 7.91 1 20 0.76 +1Score > 48 indicates identity
Score > 31 indicates homology		 AIGEK
1336 539.2680 1076.5214 1076.5098 10.8 1 20 0.37 +1Score > 42 indicates identity
Score > 28 indicates homology		 TQVSRATQGE + Deamidated (NQ)
1412 558.3033 1114.5920 1114.5982 -5.54 0 20 0.98 +1Score > 41 indicates identity
Score > 32 indicates homology		 AIDLSIDNVR
2259 501.9222 1502.7448 1502.7300 9.83 0 20 0.6 +1Score > 41 indicates identity
Score > 30 indicates homology		 MSGPNIVHSGYGLR + Oxidation (M)
1066 479.2560 956.4974 956.4927 4.96 1 20 0.51 +1Score > 40 indicates identity
Score > 29 indicates homology		 TPVAEDVAR
1543 390.2021 1167.5845 1167.5957 -9.66 1 20 0.79 +1Score > 40 indicates identity
Score > 31 indicates homology		 RSYMQILQK + 2 Deamidated (NQ)
1834 430.5891 1288.7455 1288.7391 4.96 0 20 0.54 +1Score > 35 indicates identity
Score > 30 indicates homology		 TFSIIKPDAVAK
1421 560.7951 1119.5756 1119.5924 -15.0 1 20 0.49 +1Score > 42 indicates identity
Score > 29 indicates homology		 GLQLFSVARE + Deamidated (NQ)
2153 722.8691 1443.7236 1443.7358 -8.40 1 20 0.47 +1Score > 41 indicates identity
Score > 29 indicates homology		 WDVNIGELAGITR + Deamidated (NQ)
1379 367.5345 1099.5817 1099.5734 7.51 1 20 0.45 +1Score > 41 indicates identity
Score > 29 indicates homology		 VRDSTAAAGPR
860 435.2415 868.4684 868.4766 -9.43 0 20 0.71 +1Score > 38 indicates identity
Score > 31 indicates homology		 LPDNAAIR
1422 560.8052 1119.5958 1119.5924 3.09 1 20 0.61 +1Score > 41 indicates identity
Score > 30 indicates homology		 QFLNEILSR + Deamidated (NQ)
1933 667.8621 1333.7096 1333.6990 7.99 1 20 0.17 +1Score > 40 indicates identity
Score > 24 indicates homology		 SFLVNLDRNQK + Deamidated (NQ)
1178 501.7843 1001.5540 1001.5618 -7.72 1 20 0.64 +1Score > 41 indicates identity
Score > 30 indicates homology		 TQRDLTIR
1602 594.8383 1187.6620 1187.6510 9.33 1 20 0.67 +1Score > 40 indicates identity
Score > 30 indicates homology		 LTKSQLQQNK + Deamidated (NQ)
2253 749.9406 1497.8666 1497.8515 10.1 1 20 0.75 +1Score > 35 indicates identity
Score > 31 indicates homology		 TVLKRPVDLTNDK
512 350.7042 699.3938 699.3915 3.32 0 20 0.74 +1Score > 39 indicates identity
Score > 31 indicates homology		 AGSPAIGK
556 359.1927 716.3708 716.3565 20.0 0 20 0.6 +1Score > 44 indicates identity
Score > 30 indicates homology		 AAGGAGSAR
817 852.4788 851.4715 851.4613 12.0 0 20 0.15 +1Score > 38 indicates identity
Score > 24 indicates homology		 DLGAALHR
1780 633.8365 1265.6584 1265.6503 6.43 0 20 0.07 +1Score > 40 indicates identity
Score > 21 indicates homology		 VTYDIQASLQK + Deamidated (NQ)
2801 882.4952 1762.9758 1763.0080 -18.2 1 20 0.085 +1Score > 35 indicates identity
Score > 21 indicates homology		 IIENLKPLLPAGIQDK + 2 Deamidated (NQ)
1398 554.7884 1107.5622 1107.5812 -17.1 0 20 0.58 +1Score > 41 indicates identity
Score > 30 indicates homology		 YNTLIDLQK + Deamidated (NQ)
2578 820.9201 1639.8256 1639.8279 -1.40 0 20 0.14 +1Score > 40 indicates identity
Score > 24 indicates homology		 WIAYAQSLTSKPMK + Deamidated (NQ); Oxidation (M)
877 440.2531 878.4916 878.4974 -6.54 1 20 0.59 +1Score > 38 indicates identity
Score > 30 indicates homology		 AKFSTGIR
998 466.2466 930.4786 930.4923 -14.7 0 20 0.87 +1Score > 42 indicates identity
Score > 31 indicates homology		 INPAGGFVR + Deamidated (NQ)
1830 644.3564 1286.6982 1286.7082 -7.70 0 20 0.12 +1Score > 40 indicates identity
Score > 23 indicates homology		 TVSLQALQQLGK + 2 Deamidated (NQ)
1721 617.3176 1232.6206 1232.6071 11.0 0 20 0.29 +1Score > 42 indicates identity
Score > 27 indicates homology		 QGITPLVMDSR + Deamidated (NQ); Oxidation (M)
490 345.1972 688.3798 688.3756 6.22 0 20 0.95 +1Score > 43 indicates identity
Score > 32 indicates homology		 TGVDGIK
351 616.3432 615.3359 615.3340 3.08 0 19 0.68 +1Score > 44 indicates identity
Score > 30 indicates homology		 VIDGGR
2046 696.3304 1390.6462 1390.6688 -16.2 1 19 0.3 +1Score > 40 indicates identity
Score > 27 indicates homology		 SVSNEGLTLNNSR + Deamidated (NQ)
663 386.7386 771.4626 771.4490 17.6 0 19 0.55 +1Score > 42 indicates identity
Score > 29 indicates homology		 AVVVAANK + Deamidated (NQ)
622 379.2514 756.4882 756.4858 3.26 0 19 0.83 +1Score > 39 indicates identity
Score > 31 indicates homology		 VIVTGLR
725 401.7233 801.4320 801.4344 -2.99 1 19 0.88 +1Score > 43 indicates identity
Score > 31 indicates homology		 LPKDSSR
802 423.7282 845.4418 845.4317 12.0 1 19 0.55 +1Score > 42 indicates identity
Score > 29 indicates homology		 IPDDKMK
1701 615.3381 1228.6616 1228.6775 -12.9 1 19 0.62 +1Score > 41 indicates identity
Score > 30 indicates homology		 QGKAADVAATVAK
2309 507.2900 1518.8482 1518.8294 12.4 1 19 0.77 +1Score > 37 indicates identity
Score > 31 indicates homology		 QVLFNTDEITVLK
1451 566.2867 1130.5588 1130.5642 -4.70 0 19 0.23 +1Score > 41 indicates identity
Score > 25 indicates homology		 VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
997 465.7685 929.5224 929.5182 4.62 1 19 0.91 +1Score > 42 indicates identity
Score > 31 indicates homology		 KLANADIGK + Deamidated (NQ)
367 312.1740 622.3334 622.3439 -16.7 0 19 0.077 +1Score > 41 indicates identity
Score > 21 indicates homology		 ITFSR
994 465.2775 928.5404 928.5229 18.9 1 19 0.72 +1Score > 41 indicates identity
Score > 30 indicates homology		 NLLEAQIK + Deamidated (NQ)
2354 774.3996 1546.7846 1546.8103 -16.6 1 19 0.72 +1Score > 41 indicates identity
Score > 30 indicates homology		 EAAAPGAAPAAAAAAPAAK
906 447.7389 893.4632 893.4607 2.88 0 19 0.68 +1Score > 41 indicates identity
Score > 30 indicates homology		 VTGLGQYR + Deamidated (NQ)
1663 608.8146 1215.6146 1215.5983 13.5 1 19 0.32 +1Score > 41 indicates identity
Score > 27 indicates homology		 ESLATLNSVPGE
2970 933.5015 1864.9884 1864.9934 -2.67 1 19 0.068 +1Score > 38 indicates identity
Score > 20 indicates homology		 VQYGALKIGNLKPGDYK + 2 Deamidated (NQ)
3756 594.0937 2372.3457 2372.3290 7.03 1 19 0.71 +1Score > 30 indicates identity ILGFMVLKDTQVLVNVWAIGR + Deamidated (NQ)
908 447.7393 893.4640 893.4607 3.77 1 19 0.31 +1Score > 41 indicates identity
Score > 27 indicates homology		 TVGGLEYR
239 503.3198 502.3125 502.3115 2.05 0 19 0.71 +1Score > 44 indicates identity
Score > 30 indicates homology		 ASVVK
1939 669.8270 1337.6394 1337.6649 -19.1 1 19 0.27 +1Score > 41 indicates identity
Score > 26 indicates homology		 MEFQTTLPSIR + Oxidation (M)
674 388.7098 775.4050 775.4076 -3.24 0 19 0.37 +1Score > 42 indicates identity
Score > 27 indicates homology		 QSSNVIK + Deamidated (NQ)
1548 585.8277 1169.6408 1169.6404 0.39 1 19 0.19 +1Score > 40 indicates identity
Score > 24 indicates homology		 QIVNRINALE + Deamidated (NQ)
498 346.6911 691.3676 691.3687 -1.50 1 19 0.48 +1Score > 42 indicates identity
Score > 28 indicates homology		 GKCSLK
1484 571.8093 1141.6040 1141.5866 15.3 1 19 0.76 +1Score > 40 indicates identity
Score > 30 indicates homology		 IDQIKINPAE + 2 Deamidated (NQ)
304 586.3892 585.3819 585.3737 14.0 0 19 0.71 +1Score > 39 indicates identity
Score > 30 indicates homology		 ILDIL
1446 565.8241 1129.6336 1129.6277 5.22 1 19 0.62 +1Score > 40 indicates identity
Score > 30 indicates homology		 MRTPLIDLR + Oxidation (M)
1126 491.7419 981.4692 981.4556 13.9 1 19 0.53 +1Score > 41 indicates identity
Score > 29 indicates homology		 WEQYVTR + Deamidated (NQ)
1237 515.2892 1028.5638 1028.5614 2.36 1 19 0.31 +1Score > 41 indicates identity
Score > 27 indicates homology		 LAKGAQLNGR + 2 Deamidated (NQ)
1722 411.8965 1232.6677 1232.6652 1.99 0 19 0.59 +1Score > 40 indicates identity
Score > 29 indicates homology		 TLNLLFALAQE + Deamidated (NQ)
1032 473.2810 944.5474 944.5443 3.29 0 19 0.23 +1Score > 40 indicates identity
Score > 25 indicates homology		 IYPGQVLR
2235 747.8831 1493.7516 1493.7362 10.4 0 19 0.54 +1Score > 41 indicates identity
Score > 29 indicates homology		 QVTLLGQNVNSYR + 3 Deamidated (NQ)
1878 437.2222 1308.6448 1308.6561 -8.68 1 19 0.22 +1Score > 41 indicates identity
Score > 25 indicates homology		 QNITETSFVLR + 2 Deamidated (NQ)
3231 680.6815 2039.0227 2038.9901 16.0 1 19 0.14 +1Score > 39 indicates identity
Score > 23 indicates homology		 GFGFITPESGNDLFVHFR
1290 527.7219 1053.4292 1053.4251 3.97 0 19 0.23 +1Score > 37 indicates identity
Score > 25 indicates homology		 QFNQSVSNE + 2 Deamidated (NQ)
1233 343.1945 1026.5617 1026.5498 11.5 1 19 0.081 +1Score > 39 indicates identity
Score > 20 indicates homology		 RVYDTFVK
2917 609.3018 1824.8836 1824.8927 -5.02 1 19 0.44 +1Score > 40 indicates identity
Score > 28 indicates homology		 MLQLNSFAEISTLADR + Deamidated (NQ); Oxidation (M)
3114 658.0068 1970.9986 1970.9659 16.6 0 19 0.081 +1Score > 39 indicates identity
Score > 20 indicates homology		 IGQMAQQPAAFIPDAALVE + 2 Deamidated (NQ)
1362 544.3112 1086.6078 1086.6073 0.48 0 19 0.78 +1Score > 40 indicates identity
Score > 30 indicates homology		 VPIQIQNFK + Deamidated (NQ)
1929 444.5925 1330.7557 1330.7344 16.0 1 19 0.23 +1Score > 36 indicates identity
Score > 25 indicates homology		 TTVQIKGDLLDK + Deamidated (NQ)
3422 1081.5724 2161.1302 2161.1406 -4.78 1 19 0.45 +1Score > 38 indicates identity
Score > 28 indicates homology		 FTVLDDVIEDVVTILNNLK + 2 Deamidated (NQ)
756 827.4509 826.4436 826.4436 0.0061 0 19 0.097 +1Score > 39 indicates identity
Score > 21 indicates homology		 DPIVIAAE
623 379.2515 756.4884 756.4858 3.55 0 19 0.33 +1Score > 39 indicates identity
Score > 27 indicates homology		 VIVASIR
1625 600.3402 1198.6658 1198.6670 -0.93 1 19 0.87 +1Score > 40 indicates identity
Score > 31 indicates homology		 GRNAIISLQVE
3319 1056.0525 2110.0904 2110.0946 -1.98 1 19 0.11 +1Score > 38 indicates identity
Score > 22 indicates homology		 AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
1364 545.2819 1088.5492 1088.5536 -3.99 0 19 0.9 +1Score > 42 indicates identity
Score > 31 indicates homology		 VPLTSNCGIK + Deamidated (NQ)
1555 391.5414 1171.6024 1171.5833 16.3 1 19 0.67 +1Score > 41 indicates identity
Score > 30 indicates homology		 QRNNLVLGQE + 2 Deamidated (NQ)
3170 670.7073 2009.1001 2009.1381 -18.9 1 19 0.15 +1Score > 36 indicates identity
Score > 23 indicates homology		 TSLGTINHSLLSLAALRSR
1400 554.8010 1107.5874 1107.5924 -4.48 1 19 0.9 +1Score > 40 indicates identity
Score > 31 indicates homology		 AAKFSDTIQK
2024 347.4502 1385.7717 1385.7820 -7.41 0 19 0.23 +1Score > 38 indicates identity
Score > 25 indicates homology		 WGLFQTGPVLIR
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