| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 351.7018 | 701.3890 | 701.3894 | -0.53 | 0 | 22 | 0.72 | 1Score > 42 indicates identityScore > 33 indicates homology |
VSMLPR | |
| 319.8684 | 956.5834 | 956.5728 | 11.0 | 1 | 22 | 0.31 | 1Score > 39 indicates identityScore > 30 indicates homology |
KMLPNLLK + Deamidated (NQ) | |
| 536.8010 | 1071.5874 | 1071.5924 | -4.61 | 1 | 22 | 0.95 | 1Score > 41 indicates identityScore > 35 indicates homology |
ELLLNVSGAR + Deamidated (NQ) | |
| 432.9161 | 1295.7265 | 1295.7197 | 5.21 | 1 | 22 | 0.36 | 1Score > 37 indicates identityScore > 30 indicates homology |
QGKAGGAEPLLGAK | |
| 449.7011 | 897.3876 | 897.3828 | 5.39 | 0 | 22 | 0.7 | 1Score > 38 indicates identityScore > 33 indicates homology |
SQGSSQFR + 2 Deamidated (NQ) | |
| 854.9565 | 1707.8984 | 1707.8719 | 15.5 | 1 | 22 | 0.19 | 1Score > 39 indicates identityScore > 27 indicates homology |
QNASFLYETVLPVVE | |
| 536.7878 | 1071.5610 | 1071.5560 | 4.70 | 1 | 22 | 0.87 | 1Score > 42 indicates identityScore > 34 indicates homology |
VQQALDELR + Deamidated (NQ) | |
| 589.8157 | 1177.6168 | 1177.6091 | 6.54 | 1 | 22 | 0.47 | 1Score > 41 indicates identityScore > 31 indicates homology |
RGTAVTFLGQE | |
| 483.7496 | 965.4846 | 965.4818 | 2.95 | 1 | 22 | 0.72 | 1Score > 41 indicates identityScore > 33 indicates homology |
KFQATDQK + Deamidated (NQ) | |
| 328.8550 | 983.5432 | 983.5512 | -8.14 | 1 | 22 | 0.33 | 1Score > 37 indicates identityScore > 30 indicates homology |
QAKLNRPR + 2 Deamidated (NQ) | |
| 383.6915 | 765.3684 | 765.3657 | 3.58 | 1 | 22 | 0.28 | 1Score > 42 indicates identityScore > 29 indicates homology |
VESFQR + Deamidated (NQ) | |
| 749.9398 | 1497.8650 | 1497.8515 | 9.05 | 1 | 22 | 0.74 | 1Score > 34 indicates identityScore > 33 indicates homology |
TVLKRPVDLTNDK | |
| 535.8459 | 1069.6772 | 1069.6648 | 11.7 | 0 | 22 | 0.36 | 1Score > 32 indicates identityScore > 30 indicates homology |
LIILPQAFR | |
| 553.3093 | 1104.6040 | 1104.5927 | 10.2 | 0 | 22 | 0.51 | 1Score > 40 indicates identityScore > 32 indicates homology |
AGLDALGVAYR | |
| 476.2503 | 950.4860 | 950.4821 | 4.14 | 0 | 22 | 0.42 | 1Score > 41 indicates identityScore > 31 indicates homology |
NNVEPHIK + Deamidated (NQ) | |
| 575.7940 | 1149.5734 | 1149.5666 | 5.98 | 1 | 22 | 0.53 | 1Score > 42 indicates identityScore > 32 indicates homology |
AQIDFSAEIR + Deamidated (NQ) | |
| 574.7850 | 1147.5554 | 1147.5469 | 7.43 | 1 | 22 | 0.39 | 1Score > 42 indicates identityScore > 30 indicates homology |
GESSGNLQTVR + Deamidated (NQ) | |
| 382.7206 | 763.4266 | 763.4228 | 5.03 | 1 | 22 | 0.13 | 1Score > 41 indicates identityScore > 26 indicates homology |
IIAEYR | |
| 486.7661 | 971.5176 | 971.5148 | 2.90 | 0 | 22 | 0.92 | 1Score > 42 indicates identityScore > 34 indicates homology |
SAGLQVQNR | |
| 515.3099 | 514.3026 | 514.3115 | -17.2 | 0 | 22 | 0.81 | 1Score > 35 indicates identityScore > 33 indicates homology |
ALAQL | |
| 673.3986 | 672.3913 | 672.3919 | -0.80 | 0 | 22 | 0.67 | 1Score > 43 indicates identityScore > 33 indicates homology |
GISLGAR | |
| 707.3744 | 1412.7342 | 1412.7623 | -19.9 | 1 | 22 | 0.99 | 1Score > 40 indicates identityScore > 34 indicates homology |
NGLNVIIGNVKDR + 2 Deamidated (NQ) | |
| 595.2977 | 1188.5808 | 1188.5622 | 15.7 | 0 | 22 | 0.8 | 1Score > 42 indicates identityScore > 33 indicates homology |
NTQDAAVLNGVS + Deamidated (NQ) | |
| 301.6801 | 601.3456 | 601.3547 | -15.1 | 1 | 22 | 0.67 | 1Score > 45 indicates identityScore > 33 indicates homology |
AGAKGAK | |
| 571.3336 | 1710.9790 | 1710.9767 | 1.30 | 1 | 22 | 0.43 | 1Score > 34 indicates identityScore > 31 indicates homology |
QQPITTTLELVDIIK | |
| 529.7929 | 1057.5712 | 1057.5655 | 5.43 | 1 | 22 | 0.92 | 1Score > 42 indicates identityScore > 34 indicates homology |
LDQAVAEIAK + Deamidated (NQ) | |
| 600.3378 | 1198.6610 | 1198.6418 | 16.0 | 1 | 22 | 0.5 | 1Score > 40 indicates identityScore > 31 indicates homology |
DVLALARDGNR | |
| 437.7557 | 873.4968 | 873.4920 | 5.60 | 1 | 22 | 0.31 | 1Score > 41 indicates identityScore > 29 indicates homology |
IATEIATR | |
| 1152.0696 | 2302.1246 | 2302.1176 | 3.06 | 1 | 22 | 0.063 | 1Score > 39 indicates identityScore > 22 indicates homology |
SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ) | |
| 412.7368 | 823.4590 | 823.4664 | -8.95 | 0 | 22 | 0.27 | 1Score > 38 indicates identityScore > 28 indicates homology |
HLASLAGR | |
| 602.3526 | 601.3453 | 601.3435 | 3.02 | 0 | 22 | 0.96 | 1Score > 45 indicates identityScore > 34 indicates homology |
LSAQIA | |
| 473.2494 | 944.4842 | 944.4927 | -8.94 | 0 | 22 | 0.25 | 1Score > 42 indicates identityScore > 28 indicates homology |
NGGIQLASGK + Deamidated (NQ) | |
| 533.6337 | 1597.8793 | 1597.8610 | 11.4 | 1 | 22 | 0.45 | 1Score > 38 indicates identityScore > 31 indicates homology |
SRVVDIPDLIGMQR | |
| 414.7476 | 827.4806 | 827.4752 | 6.54 | 1 | 22 | 0.64 | 1Score > 40 indicates identityScore > 32 indicates homology |
IKDNLPK + Deamidated (NQ) | |
| 497.7726 | 993.5306 | 993.5356 | -4.94 | 1 | 22 | 0.42 | 1Score > 40 indicates identityScore > 30 indicates homology |
DPKRPEPR | |
| 395.7018 | 789.3890 | 789.3868 | 2.81 | 1 | 21 | 0.97 | 1Score > 43 indicates identityScore > 34 indicates homology |
TEGAGINK + Deamidated (NQ) | |
| 308.6878 | 615.3610 | 615.3704 | -15.2 | 1 | 21 | 0.55 | 1Score > 45 indicates identityScore > 31 indicates homology |
AGGGKVK | |
| 789.4296 | 1576.8446 | 1576.8395 | 3.26 | 1 | 21 | 0.16 | 1Score > 39 indicates identityScore > 26 indicates homology |
FLGNLMQLLNNKR + Deamidated (NQ); Oxidation (M) | |
| 529.7920 | 1057.5694 | 1057.5655 | 3.74 | 1 | 21 | 0.58 | 1Score > 42 indicates identityScore > 32 indicates homology |
QQLNEILAK + 2 Deamidated (NQ) | |
| 848.3924 | 847.3851 | 847.3937 | -10.1 | 1 | 21 | 0.7 | 1Score > 41 indicates identityScore > 32 indicates homology |
DDTWRR | |
| 536.7877 | 1071.5608 | 1071.5560 | 4.51 | 1 | 21 | 0.48 | 1Score > 42 indicates identityScore > 31 indicates homology |
VQQALDELR + Deamidated (NQ) | |
| 632.8167 | 1263.6188 | 1263.6129 | 4.73 | 0 | 21 | 0.49 | 1Score > 41 indicates identityScore > 31 indicates homology |
SDALASLMNGLR + Deamidated (NQ); Oxidation (M) | |
| 554.7876 | 1107.5606 | 1107.5812 | -18.5 | 0 | 21 | 0.56 | 1Score > 41 indicates identityScore > 31 indicates homology |
YNTLIDLQK + Deamidated (NQ) | |
| 625.8334 | 1249.6522 | 1249.6740 | -17.4 | 1 | 21 | 0.4 | 1Score > 41 indicates identityScore > 30 indicates homology |
VNELIYLVMR + Deamidated (NQ) | |
| 507.2770 | 1518.8092 | 1518.8003 | 5.83 | 0 | 21 | 0.083 | 1Score > 39 indicates identityScore > 23 indicates homology |
MSVNINYLLLIPE + Deamidated (NQ) | |
| 480.2543 | 958.4940 | 958.4872 | 7.13 | 1 | 21 | 0.45 | 1Score > 43 indicates identityScore > 30 indicates homology |
LLAGEWDR | |
| 759.4420 | 2275.3042 | 2275.2940 | 4.47 | 0 | 21 | 0.38 | 1Score > 30 indicates identity |
LPVGYVQLPVGIAGPLLLDGQR + Deamidated (NQ) | |
| 401.2504 | 1200.7294 | 1200.7190 | 8.64 | 1 | 21 | 0.59 | 1Score > 32 indicates identityScore > 31 indicates homology |
TLSVNLASLKR | |
| 586.8329 | 1171.6512 | 1171.6561 | -4.13 | 1 | 21 | 0.76 | 1Score > 40 indicates identityScore > 33 indicates homology |
VAQLLETGVSR | |
| 550.3151 | 1098.6156 | 1098.6284 | -11.7 | 1 | 21 | 0.75 | 1Score > 39 indicates identityScore > 32 indicates homology |
KSADDLLIPK | |
| 360.1785 | 718.3424 | 718.3432 | -1.05 | 0 | 21 | 0.98 | 1Score > 43 indicates identityScore > 34 indicates homology |
APMSTGR | |
| 504.6085 | 1510.8037 | 1510.7932 | 6.90 | 1 | 21 | 0.1 | 1Score > 39 indicates identityScore > 24 indicates homology |
VDFPFVQRYLAR + Deamidated (NQ) | |
| 321.9280 | 1283.6829 | 1283.7085 | -19.9 | 0 | 21 | 0.86 | 1Score > 40 indicates identityScore > 33 indicates homology |
IDVVVNNAGILR + 2 Deamidated (NQ) | |
| 657.3492 | 1312.6838 | 1312.6623 | 16.4 | 1 | 21 | 0.39 | 1Score > 40 indicates identityScore > 30 indicates homology |
QGLARDGKPIQE + 2 Deamidated (NQ) | |
| 435.2484 | 868.4822 | 868.4807 | 1.80 | 0 | 21 | 0.21 | 1Score > 37 indicates identityScore > 27 indicates homology |
ILTYGFR | |
| 465.2771 | 928.5396 | 928.5229 | 18.0 | 1 | 21 | 0.61 | 1Score > 41 indicates identityScore > 31 indicates homology |
KADLLQLE | |
| 581.8034 | 1161.5922 | 1161.5812 | 9.52 | 0 | 21 | 0.68 | 1Score > 42 indicates identityScore > 32 indicates homology |
QTAAMVAAQQK + Oxidation (M) | |
| 494.7843 | 987.5540 | 987.5389 | 15.3 | 1 | 21 | 0.34 | 1Score > 41 indicates identityScore > 29 indicates homology |
LPFNRVIE + Deamidated (NQ) | |
| 467.9246 | 1400.7520 | 1400.7347 | 12.4 | 1 | 21 | 0.72 | 1Score > 40 indicates identityScore > 32 indicates homology |
MLGHANFRSIQK | |
| 732.3992 | 731.3919 | 731.3926 | -0.89 | 0 | 21 | 0.59 | 1Score > 44 indicates identityScore > 31 indicates homology |
QSIASAR | |
| 501.9201 | 1502.7385 | 1502.7148 | 15.8 | 0 | 21 | 0.4 | 1Score > 41 indicates identityScore > 30 indicates homology |
MTQVGQTHTSGSIR + Deamidated (NQ) | |
| 545.6294 | 1633.8664 | 1633.8974 | -19.0 | 1 | 21 | 0.97 | 1Score > 39 indicates identityScore > 33 indicates homology |
YLQATPMVIGIRTR + Oxidation (M) | |
| 336.2273 | 670.4400 | 670.4377 | 3.44 | 0 | 21 | 0.93 | 1Score > 37 indicates identityScore > 33 indicates homology |
VAGLAIK | |
| 388.1913 | 774.3680 | 774.3733 | -6.73 | 1 | 21 | 0.61 | 1Score > 42 indicates identityScore > 31 indicates homology |
GGSRDQR | |
| 854.9520 | 1707.8894 | 1707.8679 | 12.6 | 1 | 21 | 0.71 | 1Score > 39 indicates identityScore > 32 indicates homology |
QLQLVKDVLNHAAQE + 3 Deamidated (NQ) | |
| 531.2808 | 1590.8206 | 1590.8253 | -2.99 | 0 | 21 | 0.7 | 1Score > 40 indicates identityScore > 32 indicates homology |
LSVDGVISALAFDQR + Deamidated (NQ) | |
| 594.8353 | 1187.6560 | 1187.6510 | 4.28 | 1 | 21 | 0.82 | 1Score > 40 indicates identityScore > 32 indicates homology |
LTKSQLQQNK + Deamidated (NQ) | |
| 462.9211 | 1385.7415 | 1385.7337 | 5.64 | 0 | 21 | 0.6 | 1Score > 40 indicates identityScore > 31 indicates homology |
QITAAMQQLLPR + Deamidated (NQ); Oxidation (M) | |
| 370.7261 | 739.4376 | 739.4341 | 4.84 | 0 | 21 | 0.14 | 1Score > 33 indicates identityScore > 25 indicates homology |
AVAVAGPR | |
| 635.3849 | 1268.7552 | 1268.7565 | -0.95 | 1 | 21 | 0.96 | 1Score > 35 indicates identityScore > 33 indicates homology |
ILVQVTRNGLR + Deamidated (NQ) | |
| 380.8907 | 1139.6503 | 1139.6299 | 17.9 | 1 | 21 | 0.45 | 1Score > 37 indicates identityScore > 30 indicates homology |
SSPQLDPLRK | |
| 598.3466 | 1194.6786 | 1194.6721 | 5.52 | 1 | 21 | 0.19 | 1Score > 36 indicates identityScore > 26 indicates homology |
VAISGDKLQHK | |
| 463.2824 | 924.5502 | 924.5392 | 11.9 | 0 | 21 | 0.29 | 1Score > 36 indicates identityScore > 28 indicates homology |
GLQIAAVPR + Deamidated (NQ) | |
| 790.4502 | 1578.8858 | 1578.8729 | 8.18 | 1 | 21 | 0.39 | 1Score > 36 indicates identityScore > 29 indicates homology |
ELQANVVHLSLLSR + Deamidated (NQ) | |
| 538.2912 | 1611.8518 | 1611.8580 | -3.87 | 1 | 21 | 0.51 | 1Score > 39 indicates identityScore > 30 indicates homology |
LSARPGNEVNLLDSK | |
| 857.4458 | 1712.8770 | 1712.8886 | -6.75 | 0 | 21 | 0.19 | 1Score > 40 indicates identityScore > 26 indicates homology |
APFVDIVLGPQTYHR + Deamidated (NQ) | |
| 304.6748 | 1214.6701 | 1214.6619 | 6.76 | 1 | 21 | 0.22 | 1Score > 40 indicates identityScore > 26 indicates homology |
GAILGTQEIASR | |
| 336.7115 | 671.4084 | 671.3966 | 17.6 | 1 | 21 | 0.89 | 1Score > 44 indicates identityScore > 33 indicates homology |
VGVEIR | |
| 494.2656 | 986.5166 | 986.5033 | 13.5 | 0 | 21 | 0.22 | 1Score > 42 indicates identityScore > 27 indicates homology |
GGEPGVSGTVK | |
| 814.9664 | 1627.9182 | 1627.9144 | 2.35 | 1 | 21 | 0.14 | 1Score > 35 indicates identityScore > 25 indicates homology |
LGINASIGIANSKVAAK + 2 Deamidated (NQ) | |
| 530.3311 | 529.3238 | 529.3224 | 2.68 | 0 | 21 | 0.92 | 1Score > 47 indicates identityScore > 33 indicates homology |
AGGVVK | |
| 687.3586 | 2059.0540 | 2059.0143 | 19.3 | 1 | 21 | 0.066 | 1Score > 39 indicates identityScore > 21 indicates homology |
TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ) | |
| 446.7446 | 891.4746 | 891.4596 | 16.9 | 1 | 21 | 0.94 | 1Score > 41 indicates identityScore > 33 indicates homology |
VISMNRR + Deamidated (NQ); Oxidation (M) | |
| 434.2325 | 1299.6757 | 1299.6895 | -10.6 | 1 | 21 | 0.44 | 1Score > 41 indicates identityScore > 30 indicates homology |
SSITRDGQPALR | |
| 311.6825 | 621.3504 | 621.3486 | 2.95 | 0 | 21 | 0.12 | 1Score > 38 indicates identityScore > 24 indicates homology |
ATGFVK | |
| 495.2818 | 988.5490 | 988.5488 | 0.28 | 1 | 21 | 0.4 | 1Score > 40 indicates identityScore > 29 indicates homology |
MLLGDLRR + Oxidation (M) | |
| 345.1966 | 688.3786 | 688.3657 | 18.9 | 0 | 21 | 0.88 | 1Score > 43 indicates identityScore > 32 indicates homology |
IGTGWR | |
| 358.2274 | 714.4402 | 714.4388 | 2.01 | 0 | 21 | 0.62 | 1Score > 43 indicates identityScore > 31 indicates homology |
SVLAGLR | |
| 594.8353 | 1187.6560 | 1187.6398 | 13.7 | 0 | 20 | 0.58 | 1Score > 40 indicates identityScore > 31 indicates homology |
ITGAGTNTIVIE | |
| 648.8712 | 1295.7278 | 1295.7085 | 15.0 | 1 | 20 | 0.79 | 1Score > 37 indicates identityScore > 32 indicates homology |
INNLNHSILKK + 3 Deamidated (NQ) | |
| 793.4271 | 1584.8396 | 1584.8512 | -7.27 | 0 | 20 | 0.51 | 1Score > 39 indicates identityScore > 30 indicates homology |
TVILWDVAQGQVVR + 2 Deamidated (NQ) | |
| 1056.0530 | 2110.0914 | 2110.0946 | -1.50 | 1 | 20 | 0.059 | 1Score > 38 indicates identityScore > 21 indicates homology |
AGQDIQEVIDNLPINVPFK + Deamidated (NQ) | |
| 333.8587 | 998.5543 | 998.5436 | 10.6 | 1 | 20 | 0.31 | 1Score > 39 indicates identityScore > 28 indicates homology |
IKYGYNLK + Deamidated (NQ) | |
| 522.9443 | 1565.8111 | 1565.8011 | 6.38 | 0 | 20 | 0.4 | 1Score > 40 indicates identityScore > 29 indicates homology |
MSVNVASLDFLLIE + Oxidation (M) | |
| 579.3011 | 1156.5876 | 1156.5975 | -8.56 | 1 | 20 | 0.83 | 1Score > 41 indicates identityScore > 32 indicates homology |
LNVNLEDVLE | |
| 397.7166 | 1586.8373 | 1586.8264 | 6.88 | 1 | 20 | 0.88 | 1Score > 40 indicates identityScore > 32 indicates homology |
VDDTIQTLQQRLR + 2 Deamidated (NQ) | |
| 322.1884 | 642.3622 | 642.3701 | -12.2 | 0 | 20 | 0.48 | 1Score > 35 indicates identityScore > 30 indicates homology |
IAPGSAK | |
| 468.9145 | 1403.7217 | 1403.7369 | -10.8 | 1 | 20 | 0.29 | 1Score > 41 indicates identityScore > 27 indicates homology |
TPAKGGVTVSSSASR | |
| 365.7346 | 729.4546 | 729.4497 | 6.77 | 1 | 20 | 0.91 | 1Score > 36 indicates identityScore > 32 indicates homology |
KLSIGGR | |
| 790.4488 | 1578.8830 | 1578.8729 | 6.41 | 1 | 20 | 0.39 | 1Score > 36 indicates identityScore > 29 indicates homology |
ELQANVVHLSLLSR + Deamidated (NQ) |
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