MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
523 351.7018 701.3890 701.3894 -0.53 0 22 0.72 +1Score > 42 indicates identity
Score > 33 indicates homology
VSMLPR
1070 319.8684 956.5834 956.5728 11.0 1 22 0.31 +1Score > 39 indicates identity
Score > 30 indicates homology
KMLPNLLK + Deamidated (NQ)
1328 536.8010 1071.5874 1071.5924 -4.61 1 22 0.95 +1Score > 41 indicates identity
Score > 35 indicates homology
ELLLNVSGAR + Deamidated (NQ)
1850 432.9161 1295.7265 1295.7197 5.21 1 22 0.36 +1Score > 37 indicates identity
Score > 30 indicates homology
QGKAGGAEPLLGAK
918 449.7011 897.3876 897.3828 5.39 0 22 0.7 +1Score > 38 indicates identity
Score > 33 indicates homology
SQGSSQFR + 2 Deamidated (NQ)
2710 854.9565 1707.8984 1707.8719 15.5 1 22 0.19 +1Score > 39 indicates identity
Score > 27 indicates homology
QNASFLYETVLPVVE
1327 536.7878 1071.5610 1071.5560 4.70 1 22 0.87 +1Score > 42 indicates identity
Score > 34 indicates homology
VQQALDELR + Deamidated (NQ)
1575 589.8157 1177.6168 1177.6091 6.54 1 22 0.47 +1Score > 41 indicates identity
Score > 31 indicates homology
RGTAVTFLGQE
1095 483.7496 965.4846 965.4818 2.95 1 22 0.72 +1Score > 41 indicates identity
Score > 33 indicates homology
KFQATDQK + Deamidated (NQ)
1132 328.8550 983.5432 983.5512 -8.14 1 22 0.33 +1Score > 37 indicates identity
Score > 30 indicates homology
QAKLNRPR + 2 Deamidated (NQ)
649 383.6915 765.3684 765.3657 3.58 1 22 0.28 +1Score > 42 indicates identity
Score > 29 indicates homology
VESFQR + Deamidated (NQ)
2251 749.9398 1497.8650 1497.8515 9.05 1 22 0.74 +1Score > 34 indicates identity
Score > 33 indicates homology
TVLKRPVDLTNDK
1320 535.8459 1069.6772 1069.6648 11.7 0 22 0.36 +1Score > 32 indicates identity
Score > 30 indicates homology
LIILPQAFR
1389 553.3093 1104.6040 1104.5927 10.2 0 22 0.51 +1Score > 40 indicates identity
Score > 32 indicates homology
AGLDALGVAYR
1056 476.2503 950.4860 950.4821 4.14 0 22 0.42 +1Score > 41 indicates identity
Score > 31 indicates homology
NNVEPHIK + Deamidated (NQ)
1501 575.7940 1149.5734 1149.5666 5.98 1 22 0.53 +1Score > 42 indicates identity
Score > 32 indicates homology
AQIDFSAEIR + Deamidated (NQ)
1497 574.7850 1147.5554 1147.5469 7.43 1 22 0.39 +1Score > 42 indicates identity
Score > 30 indicates homology
GESSGNLQTVR + Deamidated (NQ)
645 382.7206 763.4266 763.4228 5.03 1 22 0.13 +1Score > 41 indicates identity
Score > 26 indicates homology
IIAEYR
1108 486.7661 971.5176 971.5148 2.90 0 22 0.92 +1Score > 42 indicates identity
Score > 34 indicates homology
SAGLQVQNR
250 515.3099 514.3026 514.3115 -17.2 0 22 0.81 +1Score > 35 indicates identity
Score > 33 indicates homology
ALAQL
456 673.3986 672.3913 672.3919 -0.80 0 22 0.67 +1Score > 43 indicates identity
Score > 33 indicates homology
GISLGAR
2096 707.3744 1412.7342 1412.7623 -19.9 1 22 0.99 +1Score > 40 indicates identity
Score > 34 indicates homology
NGLNVIIGNVKDR + 2 Deamidated (NQ)
1603 595.2977 1188.5808 1188.5622 15.7 0 22 0.8 +1Score > 42 indicates identity
Score > 33 indicates homology
NTQDAAVLNGVS + Deamidated (NQ)
319 301.6801 601.3456 601.3547 -15.1 1 22 0.67 +1Score > 45 indicates identity
Score > 33 indicates homology
AGAKGAK
2718 571.3336 1710.9790 1710.9767 1.30 1 22 0.43 +1Score > 34 indicates identity
Score > 31 indicates homology
QQPITTTLELVDIIK
1299 529.7929 1057.5712 1057.5655 5.43 1 22 0.92 +1Score > 42 indicates identity
Score > 34 indicates homology
LDQAVAEIAK + Deamidated (NQ)
1624 600.3378 1198.6610 1198.6418 16.0 1 22 0.5 +1Score > 40 indicates identity
Score > 31 indicates homology
DVLALARDGNR
872 437.7557 873.4968 873.4920 5.60 1 22 0.31 +1Score > 41 indicates identity
Score > 29 indicates homology
IATEIATR
3661 1152.0696 2302.1246 2302.1176 3.06 1 22 0.063 +1Score > 39 indicates identity
Score > 22 indicates homology
SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
754 412.7368 823.4590 823.4664 -8.95 0 22 0.27 +1Score > 38 indicates identity
Score > 28 indicates homology
HLASLAGR
318 602.3526 601.3453 601.3435 3.02 0 22 0.96 +1Score > 45 indicates identity
Score > 34 indicates homology
LSAQIA
1030 473.2494 944.4842 944.4927 -8.94 0 22 0.25 +1Score > 42 indicates identity
Score > 28 indicates homology
NGGIQLASGK + Deamidated (NQ)
2473 533.6337 1597.8793 1597.8610 11.4 1 22 0.45 +1Score > 38 indicates identity
Score > 31 indicates homology
SRVVDIPDLIGMQR
763 414.7476 827.4806 827.4752 6.54 1 22 0.64 +1Score > 40 indicates identity
Score > 32 indicates homology
IKDNLPK + Deamidated (NQ)
1150 497.7726 993.5306 993.5356 -4.94 1 22 0.42 +1Score > 40 indicates identity
Score > 30 indicates homology
DPKRPEPR
694 395.7018 789.3890 789.3868 2.81 1 21 0.97 +1Score > 43 indicates identity
Score > 34 indicates homology
TEGAGINK + Deamidated (NQ)
357 308.6878 615.3610 615.3704 -15.2 1 21 0.55 +1Score > 45 indicates identity
Score > 31 indicates homology
AGGGKVK
2426 789.4296 1576.8446 1576.8395 3.26 1 21 0.16 +1Score > 39 indicates identity
Score > 26 indicates homology
FLGNLMQLLNNKR + Deamidated (NQ); Oxidation (M)
1298 529.7920 1057.5694 1057.5655 3.74 1 21 0.58 +1Score > 42 indicates identity
Score > 32 indicates homology
QQLNEILAK + 2 Deamidated (NQ)
806 848.3924 847.3851 847.3937 -10.1 1 21 0.7 +1Score > 41 indicates identity
Score > 32 indicates homology
DDTWRR
1326 536.7877 1071.5608 1071.5560 4.51 1 21 0.48 +1Score > 42 indicates identity
Score > 31 indicates homology
VQQALDELR + Deamidated (NQ)
1777 632.8167 1263.6188 1263.6129 4.73 0 21 0.49 +1Score > 41 indicates identity
Score > 31 indicates homology
SDALASLMNGLR + Deamidated (NQ); Oxidation (M)
1397 554.7876 1107.5606 1107.5812 -18.5 0 21 0.56 +1Score > 41 indicates identity
Score > 31 indicates homology
YNTLIDLQK + Deamidated (NQ)
1753 625.8334 1249.6522 1249.6740 -17.4 1 21 0.4 +1Score > 41 indicates identity
Score > 30 indicates homology
VNELIYLVMR + Deamidated (NQ)
2307 507.2770 1518.8092 1518.8003 5.83 0 21 0.083 +1Score > 39 indicates identity
Score > 23 indicates homology
MSVNINYLLLIPE + Deamidated (NQ)
1077 480.2543 958.4940 958.4872 7.13 1 21 0.45 +1Score > 43 indicates identity
Score > 30 indicates homology
LLAGEWDR
3621 759.4420 2275.3042 2275.2940 4.47 0 21 0.38 +1Score > 30 indicates identity LPVGYVQLPVGIAGPLLLDGQR + Deamidated (NQ)
1633 401.2504 1200.7294 1200.7190 8.64 1 21 0.59 +1Score > 32 indicates identity
Score > 31 indicates homology
TLSVNLASLKR
1557 586.8329 1171.6512 1171.6561 -4.13 1 21 0.76 +1Score > 40 indicates identity
Score > 33 indicates homology
VAQLLETGVSR
1378 550.3151 1098.6156 1098.6284 -11.7 1 21 0.75 +1Score > 39 indicates identity
Score > 32 indicates homology
KSADDLLIPK
557 360.1785 718.3424 718.3432 -1.05 0 21 0.98 +1Score > 43 indicates identity
Score > 34 indicates homology
APMSTGR
2279 504.6085 1510.8037 1510.7932 6.90 1 21 0.1 +1Score > 39 indicates identity
Score > 24 indicates homology
VDFPFVQRYLAR + Deamidated (NQ)
1826 321.9280 1283.6829 1283.7085 -19.9 0 21 0.86 +1Score > 40 indicates identity
Score > 33 indicates homology
IDVVVNNAGILR + 2 Deamidated (NQ)
1884 657.3492 1312.6838 1312.6623 16.4 1 21 0.39 +1Score > 40 indicates identity
Score > 30 indicates homology
QGLARDGKPIQE + 2 Deamidated (NQ)
861 435.2484 868.4822 868.4807 1.80 0 21 0.21 +1Score > 37 indicates identity
Score > 27 indicates homology
ILTYGFR
992 465.2771 928.5396 928.5229 18.0 1 21 0.61 +1Score > 41 indicates identity
Score > 31 indicates homology
KADLLQLE
1534 581.8034 1161.5922 1161.5812 9.52 0 21 0.68 +1Score > 42 indicates identity
Score > 32 indicates homology
QTAAMVAAQQK + Oxidation (M)
1138 494.7843 987.5540 987.5389 15.3 1 21 0.34 +1Score > 41 indicates identity
Score > 29 indicates homology
LPFNRVIE + Deamidated (NQ)
2060 467.9246 1400.7520 1400.7347 12.4 1 21 0.72 +1Score > 40 indicates identity
Score > 32 indicates homology
MLGHANFRSIQK
587 732.3992 731.3919 731.3926 -0.89 0 21 0.59 +1Score > 44 indicates identity
Score > 31 indicates homology
QSIASAR
2258 501.9201 1502.7385 1502.7148 15.8 0 21 0.4 +1Score > 41 indicates identity
Score > 30 indicates homology
MTQVGQTHTSGSIR + Deamidated (NQ)
2566 545.6294 1633.8664 1633.8974 -19.0 1 21 0.97 +1Score > 39 indicates identity
Score > 33 indicates homology
YLQATPMVIGIRTR + Oxidation (M)
449 336.2273 670.4400 670.4377 3.44 0 21 0.93 +1Score > 37 indicates identity
Score > 33 indicates homology
VAGLAIK
667 388.1913 774.3680 774.3733 -6.73 1 21 0.61 +1Score > 42 indicates identity
Score > 31 indicates homology
GGSRDQR
2709 854.9520 1707.8894 1707.8679 12.6 1 21 0.71 +1Score > 39 indicates identity
Score > 32 indicates homology
QLQLVKDVLNHAAQE + 3 Deamidated (NQ)
2457 531.2808 1590.8206 1590.8253 -2.99 0 21 0.7 +1Score > 40 indicates identity
Score > 32 indicates homology
LSVDGVISALAFDQR + Deamidated (NQ)
1600 594.8353 1187.6560 1187.6510 4.28 1 21 0.82 +1Score > 40 indicates identity
Score > 32 indicates homology
LTKSQLQQNK + Deamidated (NQ)
2023 462.9211 1385.7415 1385.7337 5.64 0 21 0.6 +1Score > 40 indicates identity
Score > 31 indicates homology
QITAAMQQLLPR + Deamidated (NQ); Oxidation (M)
597 370.7261 739.4376 739.4341 4.84 0 21 0.14 +1Score > 33 indicates identity
Score > 25 indicates homology
AVAVAGPR
1789 635.3849 1268.7552 1268.7565 -0.95 1 21 0.96 +1Score > 35 indicates identity
Score > 33 indicates homology
ILVQVTRNGLR + Deamidated (NQ)
1481 380.8907 1139.6503 1139.6299 17.9 1 21 0.45 +1Score > 37 indicates identity
Score > 30 indicates homology
SSPQLDPLRK
1614 598.3466 1194.6786 1194.6721 5.52 1 21 0.19 +1Score > 36 indicates identity
Score > 26 indicates homology
VAISGDKLQHK
977 463.2824 924.5502 924.5392 11.9 0 21 0.29 +1Score > 36 indicates identity
Score > 28 indicates homology
GLQIAAVPR + Deamidated (NQ)
2428 790.4502 1578.8858 1578.8729 8.18 1 21 0.39 +1Score > 36 indicates identity
Score > 29 indicates homology
ELQANVVHLSLLSR + Deamidated (NQ)
2505 538.2912 1611.8518 1611.8580 -3.87 1 21 0.51 +1Score > 39 indicates identity
Score > 30 indicates homology
LSARPGNEVNLLDSK
2722 857.4458 1712.8770 1712.8886 -6.75 0 21 0.19 +1Score > 40 indicates identity
Score > 26 indicates homology
APFVDIVLGPQTYHR + Deamidated (NQ)
1661 304.6748 1214.6701 1214.6619 6.76 1 21 0.22 +1Score > 40 indicates identity
Score > 26 indicates homology
GAILGTQEIASR
453 336.7115 671.4084 671.3966 17.6 1 21 0.89 +1Score > 44 indicates identity
Score > 33 indicates homology
VGVEIR
1135 494.2656 986.5166 986.5033 13.5 0 21 0.22 +1Score > 42 indicates identity
Score > 27 indicates homology
GGEPGVSGTVK
2551 814.9664 1627.9182 1627.9144 2.35 1 21 0.14 +1Score > 35 indicates identity
Score > 25 indicates homology
LGINASIGIANSKVAAK + 2 Deamidated (NQ)
263 530.3311 529.3238 529.3224 2.68 0 21 0.92 +1Score > 47 indicates identity
Score > 33 indicates homology
AGGVVK
3258 687.3586 2059.0540 2059.0143 19.3 1 21 0.066 +1Score > 39 indicates identity
Score > 21 indicates homology
TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
901 446.7446 891.4746 891.4596 16.9 1 21 0.94 +1Score > 41 indicates identity
Score > 33 indicates homology
VISMNRR + Deamidated (NQ); Oxidation (M)
1855 434.2325 1299.6757 1299.6895 -10.6 1 21 0.44 +1Score > 41 indicates identity
Score > 30 indicates homology
SSITRDGQPALR
365 311.6825 621.3504 621.3486 2.95 0 21 0.12 +1Score > 38 indicates identity
Score > 24 indicates homology
ATGFVK
1141 495.2818 988.5490 988.5488 0.28 1 21 0.4 +1Score > 40 indicates identity
Score > 29 indicates homology
MLLGDLRR + Oxidation (M)
489 345.1966 688.3786 688.3657 18.9 0 21 0.88 +1Score > 43 indicates identity
Score > 32 indicates homology
IGTGWR
553 358.2274 714.4402 714.4388 2.01 0 21 0.62 +1Score > 43 indicates identity
Score > 31 indicates homology
SVLAGLR
1601 594.8353 1187.6560 1187.6398 13.7 0 20 0.58 +1Score > 40 indicates identity
Score > 31 indicates homology
ITGAGTNTIVIE
1852 648.8712 1295.7278 1295.7085 15.0 1 20 0.79 +1Score > 37 indicates identity
Score > 32 indicates homology
INNLNHSILKK + 3 Deamidated (NQ)
2445 793.4271 1584.8396 1584.8512 -7.27 0 20 0.51 +1Score > 39 indicates identity
Score > 30 indicates homology
TVILWDVAQGQVVR + 2 Deamidated (NQ)
3320 1056.0530 2110.0914 2110.0946 -1.50 1 20 0.059 +1Score > 38 indicates identity
Score > 21 indicates homology
AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
1170 333.8587 998.5543 998.5436 10.6 1 20 0.31 +1Score > 39 indicates identity
Score > 28 indicates homology
IKYGYNLK + Deamidated (NQ)
2395 522.9443 1565.8111 1565.8011 6.38 0 20 0.4 +1Score > 40 indicates identity
Score > 29 indicates homology
MSVNVASLDFLLIE + Oxidation (M)
1518 579.3011 1156.5876 1156.5975 -8.56 1 20 0.83 +1Score > 41 indicates identity
Score > 32 indicates homology
LNVNLEDVLE
2453 397.7166 1586.8373 1586.8264 6.88 1 20 0.88 +1Score > 40 indicates identity
Score > 32 indicates homology
VDDTIQTLQQRLR + 2 Deamidated (NQ)
401 322.1884 642.3622 642.3701 -12.2 0 20 0.48 +1Score > 35 indicates identity
Score > 30 indicates homology
IAPGSAK
2069 468.9145 1403.7217 1403.7369 -10.8 1 20 0.29 +1Score > 41 indicates identity
Score > 27 indicates homology
TPAKGGVTVSSSASR
584 365.7346 729.4546 729.4497 6.77 1 20 0.91 +1Score > 36 indicates identity
Score > 32 indicates homology
KLSIGGR
2427 790.4488 1578.8830 1578.8729 6.41 1 20 0.39 +1Score > 36 indicates identity
Score > 29 indicates homology
ELQANVVHLSLLSR + Deamidated (NQ)
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