MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4574 894.4999 3573.9705
4575 894.5022 3573.9797
4576 894.7454 3574.9525
4579 722.2003 3605.9651
4580 903.4821 3609.8993
4581 723.3898 3611.9126
4582 906.5016 3621.9773
4583 907.4663 3625.8361
4584 907.4669 3625.8385
4586 908.4883 3629.9241
4587 908.7493 3630.9681
4589 909.4545 3633.7889
4590 910.7756 3639.0733
4591 914.7247 3654.8697
4594 919.4651 3673.8313
4595 736.6041 3677.9841
4596 920.7559 3678.9945
4597 921.0069 3679.9985
4598 921.2592 3681.0077
4602 738.1730 3685.8286
4603 529.7144 3700.9499
4605 741.6191 3703.0591
4607 743.3846 3711.8866
4610 934.4827 3733.9017
4611 935.5139 3738.0265
4613 938.5264 3750.0765
4614 940.0163 3756.0361
4618 942.4922 3765.9397
4621 946.5104 3782.0125
4623 947.5227 3786.0617
4624 951.5043 3801.9881
4625 637.0098 3816.0151
4628 957.7604 3827.0125
4631 963.2717 3849.0577
4632 770.8218 3849.0726
4633 770.8224 3849.0756
4634 963.5334 3850.1045
4635 777.2059 3880.9931
4636 973.5069 3889.9985
4638 976.9791 3903.8873
4640 977.5439 3906.1465
4641 782.4269 3907.0981
4642 978.0409 3908.1345
4643 979.4702 3913.8517
4645 784.7711 3918.8191
4646 791.8303 3954.1151
4647 989.7889 3955.1265
4649 1002.0109 4004.0145
4651 1002.0583 4004.2041
4652 1002.2632 4005.0237
4653 1002.2872 4005.1197
4654 1002.2888 4005.1261
4655 802.0331 4005.1291
4656 803.2000 4010.9636
4657 803.2008 4010.9676
4658 1003.9971 4011.9593
4659 1003.9975 4011.9609
4660 803.4011 4011.9691
4661 804.4396 4017.1616
4662 1006.0251 4020.0713
4663 1007.7592 4027.0077
4665 1007.8084 4027.2045
4669 1016.5167 4062.0377
4670 1016.5203 4062.0521
4671 1016.5360 4062.1149
4672 1016.5432 4062.1437
4673 1016.5435 4062.1449
4674 813.4425 4062.1761
4675 1016.7847 4063.1097
4676 1017.0303 4064.0921
4677 1017.0438 4064.1461
4678 1018.2521 4068.9793
4679 1018.2531 4068.9833
4683 818.0463 4085.1951
4684 1022.3150 4085.2309
4685 1030.5439 4118.1465
4686 1030.7938 4119.1461
4687 1031.8121 4123.2193
4688 1032.0634 4124.2245
4690 826.4028 4126.9776
4692 826.4427 4127.1771
4693 1041.0553 4160.1921
4694 1043.8079 4171.2025
4695 1045.0394 4176.1285
4696 1045.5752 4178.2717
4697 837.8425 4184.1761
4700 1051.5343 4202.1081
4701 1051.5453 4202.1521
4702 1052.5493 4206.1681
4705 1064.0209 4252.0545
4706 1070.5531 4278.1833
4707 1085.5745 4338.2689
4708 868.8601 4339.2641
4709 870.2333 4346.1301
4713 1117.5894 4466.3285
4714 1121.5929 4482.3425
4715 749.3782 4490.2255
4718 902.6913 4508.4201
4719 755.3842 4526.2615
4720 1138.3531 4549.3833
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