MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4374 782.8871 3127.5193
4379 1046.2275 3135.6607
4380 628.3173 3136.5501
4381 628.3193 3136.5601
4384 1050.2290 3147.6652
4385 787.9269 3147.6785
4386 787.9275 3147.6809
4391 790.4243 3157.6681
4393 1055.5135 3163.5187
4398 792.4234 3165.6645
4400 792.4258 3165.6741
4401 792.4279 3165.6825
4404 1057.6224 3169.8454
4408 1060.5728 3178.6966
4411 796.3962 3181.5557
4413 1061.5266 3181.5580
4417 797.4349 3185.7105
4418 1063.2374 3186.6904
4420 1064.2704 3189.7894
4422 799.4002 3193.5717
4424 800.4100 3197.6109
4427 801.7003 3202.7721
4428 1068.6012 3202.7818
4429 641.5647 3202.7871
4430 801.7047 3202.7897
4432 802.1717 3204.6577
4435 1070.5599 3208.6579
4436 1070.5613 3208.6621
4438 803.4167 3209.6377
4443 1077.5520 3229.6342
4444 1078.1836 3231.5290
4445 808.8904 3231.5325
4446 1078.2535 3231.7387
4447 808.9461 3231.7553
4448 809.3013 3233.1761
4449 1079.5626 3235.6660
4450 809.9446 3235.7493
4453 810.4269 3237.6785
4456 812.6959 3246.7545
4457 1083.2595 3246.7567
4458 1084.2725 3249.7957
4459 813.4587 3249.8057
4465 816.4278 3261.6821
4467 817.4061 3265.5953
4469 817.4617 3265.8177
4473 820.4214 3277.6565
4474 1094.5577 3280.6513
4477 1099.7845 3296.3317
4479 826.4210 3301.6549
4480 826.9537 3303.7857
4483 662.1729 3305.8281
4484 1103.2762 3306.8068
4487 827.7119 3306.8185
4490 827.9627 3307.8217
4491 1105.9365 3314.7877
4496 832.6920 3326.7389
4503 1112.5858 3334.7356
4505 836.2089 3340.8065
4506 836.4542 3341.7877
4507 669.3649 3341.7881
4509 836.7016 3342.7773
4510 669.5635 3342.7811
4514 672.9670 3359.7986
4515 840.9623 3359.8201
4517 673.1671 3360.7991
4518 841.4612 3361.8157
4519 841.4664 3361.8365
4520 841.9650 3363.8309
4523 843.9410 3371.7349
4526 1130.9294 3389.7664
4527 848.4534 3389.7845
4528 1131.2667 3390.7783
4530 850.4434 3397.7445
4531 680.9672 3399.7996
4532 681.9839 3404.8831
4533 682.1848 3405.8876
4534 683.5930 3412.9286
4536 854.4586 3413.8053
4537 683.7926 3413.9266
4538 1139.2762 3414.8068
4539 684.3727 3416.8271
4540 1142.6328 3424.8766
4542 858.4384 3429.7245
4543 858.4476 3429.7613
4548 861.9843 3443.9081
4550 690.3781 3446.8541
4551 874.4958 3493.9541
4552 1168.5967 3502.7683
4553 882.2372 3524.9197
4554 706.1717 3525.8221
4555 706.3741 3526.8341
4556 1178.3038 3531.8896
4558 884.2309 3532.8945
4559 1178.6395 3532.8967
4560 884.4847 3533.9097
4565 891.4614 3561.8165
4568 893.4761 3569.8753
4569 893.4905 3569.9329
4570 893.5016 3569.9773
4571 715.0032 3569.9796
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