MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4091 911.7942 2732.3608
4092 913.1724 2736.4954
4093 913.1746 2736.5020
4094 913.5114 2737.5124
4095 913.5129 2737.5169
4096 686.6477 2742.5617
4098 916.4952 2746.4638
4103 917.8491 2750.5255
4104 688.6396 2750.5293
4105 917.8505 2750.5297
4106 917.8508 2750.5306
4107 688.6400 2750.5309
4111 690.3770 2757.4789
4113 690.3883 2757.5241
4114 920.1840 2757.5302
4119 555.0928 2770.4276
4123 925.8450 2774.5132
4124 925.8463 2774.5171
4127 926.5084 2776.5034
4128 926.5168 2776.5286
4129 926.5464 2776.6174
4135 929.8702 2786.5888
4136 697.8685 2787.4449
4138 930.5112 2788.5118
4140 698.3737 2789.4657
4144 931.5272 2791.5598
4152 936.5236 2806.5490
4153 936.8541 2807.5405
4161 703.1408 2808.5341
4165 938.5092 2812.5058
4169 564.2847 2816.3871
4170 940.5108 2818.5106
4173 705.8512 2819.3757
4175 941.1416 2820.4030
4177 706.4501 2821.7713
4178 941.8472 2822.5198
4181 944.5234 2830.5484
4186 948.5036 2842.4890
4189 948.8859 2843.6359
4191 712.9031 2847.5833
4195 952.8804 2855.6194
4197 716.8563 2863.3961
4198 955.8824 2864.6254
4199 956.1971 2865.5695
4204 574.5334 2867.6306
4209 718.8931 2871.5433
4210 958.5490 2872.6252
4211 719.1636 2872.6253
4212 958.5492 2872.6258
4213 959.1777 2874.5113
4218 721.1534 2880.5845
4222 963.2194 2886.6364
4224 723.6377 2890.5217
4229 965.4926 2893.4560
4235 726.4104 2901.6125
4236 726.4145 2901.6289
4237 968.5182 2902.5328
4239 969.8534 2906.5384
4242 728.1327 2908.5017
4243 728.1331 2908.5033
4244 970.5099 2908.5079
4245 970.5142 2908.5208
4252 975.5236 2923.5490
4258 733.3979 2929.5625
4269 984.1959 2949.5659
4271 984.5284 2950.5634
4278 988.4933 2962.4581
4279 741.8735 2963.4649
4281 742.3892 2965.5277
4284 990.5214 2968.5424
4285 990.5293 2968.5661
4287 995.5213 2983.5421
4291 748.4024 2989.5805
4295 998.5001 2992.4785
4296 998.5012 2992.4818
4297 998.5058 2992.4956
4303 1002.1168 3003.3286
4308 1004.5471 3010.6195
4311 1006.5807 3016.7203
4312 755.3890 3017.5269
4315 756.0962 3020.3557
4320 1010.5623 3028.6651
4322 1010.5671 3028.6795
4329 760.4373 3037.7201
4330 760.6562 3038.5957
4331 761.9259 3043.6745
4334 1017.5734 3049.6984
4335 763.4384 3049.7245
4336 1018.8365 3053.4877
4340 1021.5287 3061.5643
4343 1023.2253 3066.6541
4347 1024.2377 3069.6913
4350 771.3940 3081.5469
4351 771.4510 3081.7749
4353 1028.5283 3082.5631
4356 1030.2386 3087.6940
4357 1030.5078 3088.5016
4365 1033.8801 3098.6185
4367 1035.8944 3104.6614
4369 779.4268 3113.6781
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