MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3562 746.7274 2237.1604
3573 748.4190 2242.2352
3576 1122.6190 2243.2234
3580 749.4072 2245.1998
3582 562.5619 2246.2185
3583 750.0922 2247.2548
3589 750.7241 2249.1505
3592 751.4356 2251.2850
3616 758.4065 2272.1977
3633 762.7831 2285.3275
3636 764.3936 2290.1590
3647 766.4343 2296.2811
3653 767.7563 2300.2471
3688 463.6559 2313.2431
3696 773.3961 2317.1665
3711 777.4176 2329.2310
3716 779.7172 2336.1298
3724 1173.6929 2345.3712
3725 1173.6965 2345.3784
3726 782.8001 2345.3785
3727 782.8002 2345.3788
3733 785.4474 2353.3204
3735 786.4232 2356.2478
3737 786.4409 2356.3009
3741 787.4211 2359.2415
3753 790.7981 2369.3725
3775 1195.2109 2388.4072
3779 798.0959 2391.2659
3782 599.5822 2394.2997
3790 602.0775 2404.2809
3798 805.1279 2412.3619
3800 805.1300 2412.3682
3801 805.8020 2414.3842
3814 605.3256 2417.2733
3821 808.4605 2422.3597
3837 611.2973 2441.1601
3838 611.3729 2441.4625
3842 816.4252 2446.2538
3847 818.4302 2452.2688
3856 823.1368 2466.3886
3857 823.1394 2466.3964
3861 824.4433 2470.3081
3869 827.1145 2478.3217
3874 827.4655 2479.3747
3876 827.7968 2480.3686
3879 622.5806 2486.2933
3880 830.1428 2487.4066
3881 622.8601 2487.4113
3885 623.1091 2488.4073
3899 628.3356 2509.3133
3907 839.1433 2514.4081
3908 839.1473 2514.4201
3916 842.1383 2523.3931
3920 633.5574 2530.2005
3921 844.4263 2530.2571
3935 849.8085 2546.4037
3936 849.8373 2546.4901
3943 851.1468 2550.4186
3944 851.1512 2550.4318
3948 852.4360 2554.2862
3950 639.5920 2554.3389
3951 853.7528 2558.2366
3960 643.0673 2568.2401
3965 645.3333 2577.3041
3966 645.3350 2577.3109
3972 861.1504 2580.4294
3982 650.3504 2597.3725
3986 870.1324 2607.3754
3988 653.5679 2610.2425 2610.2061 14.0 0     +0Score > 38 indicates identity
Score > 13 indicates homology		 IFTSLHMDHYDIAVQNTQYGLE + Oxidation (M)
3991 873.7429 2618.2069
3994 874.4805 2620.4197
3995 875.4971 2623.4695
3999 658.8701 2631.4513
4002 879.1203 2634.3391
4006 879.4174 2635.2304
4007 879.4175 2635.2307
4009 880.4652 2638.3738
4012 882.7986 2645.3740
4013 662.5972 2646.3597
4020 885.4439 2653.3099
4021 887.1818 2658.5236
4024 888.9980 2663.9722
4026 890.1634 2667.4684
4028 890.4908 2668.4506
4035 893.8148 2678.4226
4036 894.4827 2680.4263
4037 895.1942 2682.5608
4048 898.4266 2692.2580
4061 676.3736 2701.4653
4068 902.8218 2705.4436
4070 903.4116 2707.2130
4077 907.5349 2719.5829
4078 907.5486 2719.6240
4080 907.8665 2720.5777
4081 908.0815 2721.2227
4082 908.0829 2721.2269
4083 908.4700 2722.3882
4086 683.3973 2729.5601
4087 910.8612 2729.5618
4088 910.8617 2729.5633
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