MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
481 683.8626 682.8553
483 684.3232 683.3159
531 703.0443 702.0370
614 752.3866 751.3793
647 765.4308 764.4235
703 793.9296 792.9223
726 803.4143 802.4070
727 803.4213 802.4140
729 806.9138 805.9065
730 806.9395 805.9322
743 817.9413 816.9340
755 827.1324 826.1251
779 837.1248 836.1175
821 852.5754 851.5681
835 857.9470 856.9397
864 872.4447 871.4374
952 916.4655 915.4582
1053 950.4948 949.4875
1133 985.5156 984.5083
1154 995.0134 994.0061
1241 1030.8983 1029.8910
1287 1051.5491 1050.5418
1341 1080.0641 1079.0568
1496 382.9266 1145.7580
1577 589.9854 1177.9562
1627 600.5700 1199.1254
1705 615.6107 1229.2068
1710 616.5853 1231.1560
1733 620.1177 1238.2208
1838 430.9362 1289.7868
2037 463.9592 1388.8558
2122 714.4255 1426.8364
2328 383.9889 1531.9265
2408 524.6445 1570.9117
2413 787.4844 1572.9542
2422 787.9860 1573.9574
2434 790.9679 1579.9212
2486 536.3153 1605.9241
2500 805.5114 1609.0082
2563 544.9941 1631.9605
2596 550.9858 1649.9356
2602 827.9602 1653.9058
2667 562.6561 1684.9465
2723 572.2847 1713.8323
2732 861.2039 1720.3932
2739 862.0358 1722.0570
2769 867.5024 1732.9902
2787 879.4037 1756.7928
2793 587.6793 1760.0161
2794 587.6921 1760.0545
2822 887.7321 1773.4496
2829 593.3389 1776.9949
2904 455.4710 1817.8549
3033 637.3635 1909.0687
3060 643.6915 1928.0527
3063 644.3643 1930.0711
3068 966.5475 1931.0804
3074 645.6914 1934.0524
3109 656.3807 1966.1203
3118 658.0427 1971.1063
3131 992.5804 1983.1462
3163 669.7298 2006.1676
3166 670.0625 2007.1657
3167 670.0640 2007.1702
3168 670.3972 2008.1698
3188 672.7128 2015.1166
3194 674.0565 2019.1477
3205 675.4105 2023.2097
3247 685.6782 2054.0128
3251 686.7225 2057.1457
3269 692.7203 2075.1391
3270 692.7252 2075.1538
3272 520.3151 2077.2313
3273 693.4190 2077.2352
3302 699.3730 2095.0972
3315 1055.5232 2109.0318
3324 704.7350 2111.1832
3332 1058.5903 2115.1660
3350 1061.0526 2120.0906
3362 710.4183 2128.2331
3363 710.4203 2128.2391
3367 712.0566 2133.1480
3380 713.7195 2138.1367
3384 714.3970 2140.1692
3397 716.4003 2146.1791
3398 716.4134 2146.2184
3431 724.0669 2169.1789
3432 1085.5995 2169.1844
3453 545.8271 2179.2793
3455 546.2841 2181.1073
3463 1092.5845 2183.1544
3505 735.0825 2202.2257
3523 737.4230 2209.2472
3528 553.8255 2211.2729
3531 739.7593 2216.2561
3540 742.7313 2225.1721
3541 743.0644 2226.1714
3542 558.0551 2228.1913
3550 744.7490 2231.2252
3560 1119.5830 2237.1514
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