MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
150 416.2637 415.2564
151 416.2641 415.2568
152 416.2882 415.2809
153 416.2883 415.2810
154 416.2886 415.2813
155 416.2887 415.2814
156 416.2890 415.2817
162 418.2675 417.2602
163 421.2573 420.2500
164 421.2577 420.2504
165 423.2624 422.2551
166 423.2946 422.2873
167 425.2389 424.2316
168 425.2393 424.2320
169 425.2396 424.2323
170 428.2887 427.2814
171 429.7435 428.7362
172 430.2798 429.2725
173 430.3037 429.2964
174 430.3043 429.2970
175 430.3046 429.2973
176 430.3047 429.2974
177 430.8589 429.8516
178 432.2227 431.2154
179 432.2593 431.2520
180 432.2606 431.2533
181 437.2169 436.2096
182 439.7421 438.7348
183 440.2529 439.2456
184 440.7316 439.7243
185 442.7407 441.7334
186 443.2508 442.2435
187 444.2946 443.2873
188 444.2947 443.2874
189 444.2951 443.2878
190 444.3202 443.3129
191 446.2739 445.2666
192 446.2742 445.2669
193 446.2749 445.2676
194 448.2569 447.2496
195 450.2485 449.2412
196 450.7825 449.7752
197 451.2682 450.2609
198 451.2685 450.2612
199 451.2687 450.2614
200 454.8324 453.8251
201 458.3105 457.3032
202 458.3118 457.3045
204 459.2656 458.2583
205 459.2659 458.2586
206 460.2901 459.2828
207 460.3144 459.3071
208 462.2296 461.2223
209 462.2363 461.2290
210 467.7739 466.7666
211 468.2764 467.2691
212 471.7733 470.7660
213 472.3260 471.3187
221 475.2626 474.2553
222 475.9602 474.9529
223 476.2110 475.2037
224 484.3298 483.3225
225 486.7610 485.7537
233 489.9493 488.9420
234 496.2845 495.2772
243 506.3103 505.3030
244 506.3106 505.3033
245 506.7802 505.7729
246 510.2368 509.2295
247 510.2388 509.2315
248 514.8030 513.7957
256 516.3134 515.3061
260 522.2714 521.2641
261 524.2371 523.2298
268 533.1211 532.1138
269 536.3151 535.3078
274 549.3043 548.2970
275 552.8259 551.8186
276 555.1042 554.0969
277 555.1072 554.0999
278 557.0799 556.0726
279 557.0831 556.0758
288 567.3033 566.2960
289 569.1198 568.1125
291 571.0687 570.0614
292 571.0718 570.0645
297 575.9763 574.9690
299 581.8292 580.8219
311 590.8304 589.8231
312 599.6764 598.6691
313 600.0114 599.0041
335 609.3525 608.3452
339 610.8379 609.8306
340 611.0034 609.9961
361 617.6303 616.6230
363 618.8086 617.8013
366 622.8218 621.8145
369 623.3666 622.3593
392 634.2927 633.2854
414 647.9130 646.9057
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