MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
48 345.2257 344.2184
49 345.2257 344.2184
50 345.2263 344.2190
51 347.2301 346.2228
52 347.2303 346.2230
53 349.2238 348.2165
54 351.2186 350.2113
55 351.2391 350.2318
56 353.2552 352.2479
57 354.1803 353.1730
58 357.2512 356.2439
59 358.2462 357.2389
60 359.2049 358.1976
61 359.2411 358.2338
62 359.2411 358.2338
63 359.2411 358.2338
64 359.2413 358.2340
65 359.2416 358.2343
66 359.2417 358.2344
67 359.2419 358.2346
68 359.2438 358.2365
69 359.2658 358.2585
70 359.2663 358.2590
71 360.2256 359.2183
72 361.1997 360.1924
73 361.1999 360.1926
74 361.2207 360.2134
75 365.2234 364.2161
76 367.2343 366.2270
77 371.3176 370.3103
78 372.7232 371.7159
79 373.2828 372.2755
80 373.2829 372.2756
81 374.2292 373.2219
82 374.2296 373.2223
83 375.2005 374.1932
84 375.2247 374.2174
85 375.2253 374.2180
86 375.2366 374.2293
87 375.2374 374.2301
88 375.2375 374.2302
89 376.2643 375.2570
90 379.2108 378.2035
91 380.7206 379.7133
92 385.7305 384.7232
93 385.7493 384.7420
94 386.2411 385.2338
95 386.2419 385.2346
96 387.2724 386.2651
97 387.2727 386.2654
98 387.2729 386.2656
99 387.2739 386.2666
100 387.2740 386.2667
101 388.2319 387.2246
102 388.2576 387.2503
103 388.2579 387.2506
104 389.2387 388.2314
105 389.2397 388.2324
106 389.2405 388.2332
107 389.2531 388.2458
108 389.2540 388.2467
109 393.2487 392.2414
110 393.2513 392.2440
111 398.2469 397.2396
112 399.2371 398.2298
113 399.7592 398.7519
114 400.7366 399.7293
115 401.2521 400.2448
116 401.2873 400.2800
117 401.2881 400.2808
118 401.2884 400.2811
119 401.2885 400.2812
120 401.2885 400.2812
121 401.2893 400.2820
122 401.7262 400.7189
123 402.2173 401.2100
124 402.2476 401.2403
125 402.2477 401.2404
126 402.2481 401.2408
127 403.2321 402.2248
128 403.2321 402.2248
131 404.2520 403.2447
132 407.2276 406.2203
133 407.2278 406.2205
134 407.2278 406.2205
135 407.2279 406.2206
136 407.2282 406.2209
137 407.2284 406.2211
138 407.7448 406.7375
139 409.2440 408.2367
140 409.2440 408.2367
141 409.2441 408.2368
142 409.2442 408.2369
143 409.2443 408.2370
144 409.2447 408.2374
145 414.2418 413.2345
146 414.2729 413.2656
147 416.2629 415.2556
148 416.2631 415.2558
149 416.2634 415.2561
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