MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
281 558.3632 557.3559 557.3537 4.02 0 25 0.82 +1Score > 40 indicates identity
Score > 37 indicates homology		 AGAVLK
1181 502.2818 1002.5490 1002.5346 14.5 0 25 0.49 +1Score > 41 indicates identity
Score > 34 indicates homology		 VTNVAGINSK + Deamidated (NQ)
2197 491.9370 1472.7892 1472.8100 -14.1 0 25 0.66 +1Score > 40 indicates identity
Score > 36 indicates homology		 HGLPPTAGLGIGIDR
2191 490.9285 1469.7637 1469.7627 0.67 1 25 0.094 +1Score > 40 indicates identity
Score > 27 indicates homology		 HSVYGGVVPELASR
264 531.2908 530.2835 530.2813 4.29 0 25 0.78 +1Score > 46 indicates identity
Score > 36 indicates homology		 AGQGAK
873 438.7525 875.4904 875.4865 4.53 1 25 0.7 +1Score > 40 indicates identity
Score > 36 indicates homology		 LFAEQIR
1798 425.5435 1273.6087 1273.6262 -13.8 1 25 0.48 +1Score > 41 indicates identity
Score > 34 indicates homology		 QATQRNAVNIR + 4 Deamidated (NQ)
504 347.6936 693.3726 693.3810 -12.0 0 25 0.16 +1Score > 40 indicates identity
Score > 30 indicates homology		 IFSATR
1750 625.3284 1248.6422 1248.6350 5.80 1 25 0.35 +1Score > 41 indicates identity
Score > 33 indicates homology		 ATFLQVRVQGE + 2 Deamidated (NQ)
2447 793.4338 1584.8530 1584.8519 0.74 1 25 0.25 +1Score > 39 indicates identity
Score > 31 indicates homology		 VVMLLTAGGGGGAGGGRR
1522 387.2050 1158.5932 1158.5993 -5.29 1 25 0.75 +1Score > 42 indicates identity
Score > 36 indicates homology		 GKQQDTVLGGR + Deamidated (NQ)
625 380.2346 758.4546 758.4538 1.12 0 25 0.38 +1Score > 41 indicates identity
Score > 33 indicates homology		 TIVADLK
660 386.6972 771.3798 771.3875 -9.94 0 25 0.53 +1Score > 40 indicates identity
Score > 35 indicates homology		 LNGTGGPR + Deamidated (NQ)
1219 339.5303 1015.5691 1015.5550 13.9 1 25 0.68 +1Score > 40 indicates identity
Score > 36 indicates homology		 SVGEAQVVVK + Deamidated (NQ)
895 444.2592 886.5038 886.5123 -9.58 0 25 0.84 +1Score > 42 indicates identity
Score > 37 indicates homology		 NALNLTLK + Deamidated (NQ)
391 316.7038 631.3930 631.3905 4.08 0 25 0.26 +1Score > 31 indicates identity ITTIGK
664 772.4706 771.4633 771.4715 -10.6 1 25 0.23 +1Score > 42 indicates identity
Score > 31 indicates homology		 RLVATGR
2277 755.9497 1509.8848 1509.8654 12.9 0 25 0.13 +1Score > 33 indicates identity
Score > 28 indicates homology		 LIVLTDTPGILLNE
2042 464.2614 1389.7624 1389.7504 8.65 1 25 0.27 +1Score > 39 indicates identity
Score > 31 indicates homology		 GLFVAISNQEIAK + Deamidated (NQ)
1689 613.3329 1224.6512 1224.6462 4.10 1 25 0.61 +1Score > 39 indicates identity
Score > 35 indicates homology		 QVAELINADPR
488 344.7065 687.3984 687.3915 10.1 0 25 0.84 +1Score > 42 indicates identity
Score > 36 indicates homology		 GQIITR + Deamidated (NQ)
1368 547.2847 1092.5548 1092.5564 -1.39 1 25 0.67 +1Score > 42 indicates identity
Score > 35 indicates homology		 LAGEFTQATR
871 437.7552 873.4958 873.4807 17.3 1 25 0.92 +1Score > 41 indicates identity
Score > 37 indicates homology		 LAEDVSIK
3663 768.3839 2302.1299 2302.1176 5.32 1 25 0.31 +1Score > 39 indicates identity
Score > 32 indicates homology		 SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
465 338.1837 674.3528 674.3599 -10.5 0 25 0.85 +1Score > 43 indicates identity
Score > 36 indicates homology		 DGVSAVK
1004 468.2762 934.5378 934.5276 10.9 0 25 0.085 +1Score > 37 indicates identity
Score > 26 indicates homology		 NFLIPFGK
1028 472.7952 943.5758 943.5702 5.96 1 25 0.44 +1Score > 36 indicates identity
Score > 33 indicates homology		 TITVLIER
1140 330.5143 988.5211 988.5189 2.18 0 24 0.61 +1Score > 42 indicates identity
Score > 35 indicates homology		 SQIITDGVR + Deamidated (NQ)
2719 856.9720 1711.9294 1711.9581 -16.7 1 24 0.2 +1Score > 38 indicates identity
Score > 30 indicates homology		 DNITVTVLGVKGNQVR
1647 606.3456 1210.6766 1210.6822 -4.59 1 24 0.8 +1Score > 38 indicates identity
Score > 36 indicates homology		 QSILLRYYR
1630 601.3408 1200.6670 1200.6503 14.0 1 24 0.32 +1Score > 40 indicates identity
Score > 32 indicates homology		 SWALVDILER
685 392.7701 783.5256 783.5218 4.90 0 24 0.31 +1Score > 32 indicates identity IIAQIVK
1902 660.8270 1319.6394 1319.6622 -17.2 1 24 0.39 +1Score > 42 indicates identity
Score > 33 indicates homology		 INQQAFQWKR + 2 Deamidated (NQ)
937 454.7470 907.4794 907.4651 15.9 1 24 0.71 +1Score > 41 indicates identity
Score > 35 indicates homology		 GANLEYLK + Deamidated (NQ)
1253 518.2808 1034.5470 1034.5318 14.8 1 24 0.89 +1Score > 41 indicates identity
Score > 36 indicates homology		 MLQEIGSIK + Deamidated (NQ); Oxidation (M)
1749 625.3273 1248.6400 1248.6350 4.03 1 24 0.35 +1Score > 41 indicates identity
Score > 32 indicates homology		 ATFLQVRVQGE + 2 Deamidated (NQ)
1238 343.8708 1028.5906 1028.5866 3.87 1 24 0.81 +1Score > 40 indicates identity
Score > 36 indicates homology		 EVNLAVSGIK
3132 662.3671 1984.0795 1984.0531 13.3 1 24 0.17 +1Score > 36 indicates identity
Score > 29 indicates homology		 IGAIHSVVFGGFSPEALAGR
1454 566.2877 1130.5608 1130.5567 3.63 1 24 0.74 +1Score > 42 indicates identity
Score > 36 indicates homology		 VVELNASDQR + Deamidated (NQ)
690 394.7191 787.4236 787.4188 6.19 0 24 0.59 +1Score > 42 indicates identity
Score > 35 indicates homology		 AQAALASR + Deamidated (NQ)
1752 625.8332 1249.6518 1249.6740 -17.7 1 24 0.31 +1Score > 41 indicates identity
Score > 32 indicates homology		 VNELIYLVMR + Deamidated (NQ)
2278 755.9504 1509.8862 1509.8654 13.8 0 24 0.13 +1Score > 32 indicates identity
Score > 28 indicates homology		 LIVLTDTPGILLNE
1270 521.7900 1041.5654 1041.5455 19.2 0 24 0.97 +1Score > 40 indicates identity
Score > 37 indicates homology		 QLPSDNGLAK
670 388.6940 775.3734 775.3864 -16.7 0 24 0.87 +1Score > 43 indicates identity
Score > 36 indicates homology		 WNSALGK + Deamidated (NQ)
424 329.7111 657.4076 657.4061 2.33 0 24 0.25 +1Score > 41 indicates identity
Score > 31 indicates homology		 IDVALK
1558 586.8333 1171.6520 1171.6448 6.17 1 24 0.88 +1Score > 40 indicates identity
Score > 36 indicates homology		 NLSAAVLDELK
1300 529.8030 1057.5914 1057.5768 13.9 1 24 0.26 +1Score > 40 indicates identity
Score > 31 indicates homology		 QTQAILGAEK
2816 886.9536 1771.8926 1771.8815 6.32 1 24 0.26 +1Score > 40 indicates identity
Score > 31 indicates homology		 VNFSVNFKCLSSQIK + 2 Deamidated (NQ)
1192 505.7329 1009.4512 1009.4464 4.76 1 24 0.97 +1Score > 40 indicates identity
Score > 37 indicates homology		 QYANDRNK + 2 Deamidated (NQ)
1248 345.5033 1033.4881 1033.4862 1.78 1 24 0.17 +1Score > 41 indicates identity
Score > 29 indicates homology		 EMDAGVSGLR
1751 625.8320 1249.6494 1249.6740 -19.7 1 24 0.3 +1Score > 41 indicates identity
Score > 31 indicates homology		 VNELIYLVMR + Deamidated (NQ)
1648 606.3466 1210.6786 1210.6822 -2.94 1 24 0.5 +1Score > 37 indicates identity
Score > 34 indicates homology		 QSILLRYYR
3613 758.0759 2271.2059 2271.1747 13.7 1 24 0.27 +1Score > 37 indicates identity
Score > 31 indicates homology		 LFGSIGTHDIADALTASGVEVAK
1734 620.3255 1238.6364 1238.6394 -2.41 0 24 0.17 +1Score > 40 indicates identity
Score > 29 indicates homology		 VTASLPTPVQPE + Deamidated (NQ)
1942 447.9221 1340.7445 1340.7664 -16.3 0 24 0.17 +1Score > 39 indicates identity
Score > 29 indicates homology		 VTVPDLSALAVTR
2435 791.3893 1580.7640 1580.7769 -8.16 1 24 0.38 +1Score > 41 indicates identity
Score > 32 indicates homology		 QDIQKLFSTWCR
1072 319.8686 956.5840 956.5728 11.6 1 24 0.74 +1Score > 39 indicates identity
Score > 35 indicates homology		 KMLPNLLK + Deamidated (NQ)
656 385.2545 768.4944 768.4857 11.3 0 24 0.084 +1Score > 31 indicates identity
Score > 26 indicates homology		 AAAKPALK
775 417.7471 833.4796 833.4759 4.47 0 24 0.35 +1Score > 39 indicates identity
Score > 32 indicates homology		 ITNAFLR
331 302.6880 603.3614 603.3592 3.79 0 24 0.64 +1Score > 40 indicates identity
Score > 34 indicates homology		 ISSGLK
517 351.6927 701.3708 701.3821 -16.0 0 24 0.36 +1Score > 44 indicates identity
Score > 32 indicates homology		 GGGGSLVR
1321 357.8467 1070.5183 1070.5356 -16.2 1 24 0.64 +1Score > 40 indicates identity
Score > 34 indicates homology		 KNIDPQNNK + Deamidated (NQ)
780 419.2499 836.4852 836.4830 2.72 0 24 0.25 +1Score > 36 indicates identity
Score > 30 indicates homology		 LIAVYMK
1889 439.2501 1314.7285 1314.7143 10.8 1 24 0.17 +1Score > 40 indicates identity
Score > 29 indicates homology		 IVLNIGSGAESVR + Deamidated (NQ)
458 673.4005 672.3932 672.3918 2.05 1 24 0.88 +1Score > 43 indicates identity
Score > 36 indicates homology		 KQIQR + Deamidated (NQ)
1106 486.7562 971.4978 971.5036 -5.92 1 24 0.8 +1Score > 41 indicates identity
Score > 35 indicates homology		 VGIEAVGNGR + Deamidated (NQ)
265 531.3174 530.3101 530.3176 -14.1 1 23 0.77 +1Score > 47 indicates identity
Score > 35 indicates homology		 KNAAK
1947 672.4131 1342.8116 1342.8336 -16.4 1 23 0.26 +1Score > 30 indicates identity
Score > 30 indicates homology		 GTKIYLLAPIVR
1258 1035.5894 1034.5821 1034.6012 -18.4 1 23 0.92 +1Score > 38 indicates identity
Score > 36 indicates homology		 IEIYIVGTK
413 324.1685 646.3224 646.3187 5.81 0 23 0.82 +1Score > 44 indicates identity
Score > 35 indicates homology		 SWQAR
1441 564.8224 1127.6302 1127.6299 0.34 0 23 0.23 +1Score > 39 indicates identity
Score > 29 indicates homology		 LQGQNVLTVR + Deamidated (NQ)
1276 522.3077 1042.6008 1042.6022 -1.33 1 23 0.32 +1Score > 39 indicates identity
Score > 31 indicates homology		 VSLNIVEIR + Deamidated (NQ)
354 616.3442 615.3369 615.3340 4.73 0 23 0.43 +1Score > 44 indicates identity
Score > 32 indicates homology		 VSPSAR
900 446.7438 891.4730 891.4814 -9.37 1 23 0.56 +1Score > 41 indicates identity
Score > 33 indicates homology		 VAEFLSAR
1562 586.8528 1171.6910 1171.6812 8.39 1 23 0.71 +1Score > 36 indicates identity
Score > 34 indicates homology		 KIGTGINDLLK + Deamidated (NQ)
3412 718.3851 2152.1335 2152.1198 6.35 1 23 0.67 +1Score > 37 indicates identity
Score > 34 indicates homology		 TGQTIIAGMGELHLDIIVDR + Deamidated (NQ)
1033 473.7558 945.4970 945.5131 -17.0 1 23 0.43 +1Score > 42 indicates identity
Score > 32 indicates homology		 TNVGEISVK
3287 696.3447 2086.0123 2085.9816 14.7 1 23 0.18 +1Score > 39 indicates identity
Score > 28 indicates homology		 ALDTYNEINAVMNLVLFE + 2 Deamidated (NQ); Oxidation (M)
1199 337.8674 1010.5804 1010.5913 -10.8 0 23 0.16 +1Score > 36 indicates identity
Score > 27 indicates homology		 QWILNPLK
2059 701.3830 1400.7514 1400.7623 -7.77 1 23 0.57 +1Score > 40 indicates identity
Score > 33 indicates homology		 SVTQVNRDLLAGK + Deamidated (NQ)
1468 567.8256 1133.6366 1133.6233 11.7 1 23 0.39 +1Score > 38 indicates identity
Score > 31 indicates homology		 FGLIPEFVGR
344 307.6982 613.3818 613.3911 -15.2 0 23 0.72 +1Score > 38 indicates identity
Score > 34 indicates homology		 IGGVLR
846 430.7473 859.4800 859.4837 -4.25 1 23 0.46 +1Score > 42 indicates identity
Score > 32 indicates homology		 MIEVGALK
353 308.6757 615.3368 615.3452 -13.6 1 23 0.78 +1Score > 44 indicates identity
Score > 34 indicates homology		 AGNKAR
1179 502.2581 1002.5016 1002.4982 3.47 0 23 0.79 +1Score > 42 indicates identity
Score > 34 indicates homology		 IANISGDGQK + Deamidated (NQ)
2342 771.9243 1541.8340 1541.8123 14.1 1 23 0.36 +1Score > 39 indicates identity
Score > 31 indicates homology		 AAVINNAMLEAILAE
627 380.2356 758.4566 758.4538 3.76 1 23 0.61 +1Score > 40 indicates identity
Score > 33 indicates homology		 TLVIGEK
991 310.5131 928.5175 928.5130 4.78 1 23 0.62 +1Score > 41 indicates identity
Score > 33 indicates homology		 IYEHVLR
1185 503.2988 1004.5830 1004.5767 6.34 1 22 0.29 +1Score > 38 indicates identity
Score > 30 indicates homology		 RYLINGIR + Deamidated (NQ)
1596 594.3209 1186.6272 1186.6346 -6.18 1 22 0.77 +1Score > 41 indicates identity
Score > 34 indicates homology		 AKASLIAQAWE
592 367.7152 733.4158 733.4123 4.89 1 22 0.92 +1Score > 39 indicates identity
Score > 35 indicates homology		 VEALFR
1893 439.8836 1316.6290 1316.6096 14.7 0 22 0.77 +1Score > 41 indicates identity
Score > 34 indicates homology		 NATSDILVSPGNE + Deamidated (NQ)
455 336.7132 671.4118 671.4078 5.97 1 22 0.27 +1Score > 42 indicates identity
Score > 29 indicates homology		 AGAKGIR
467 338.1880 674.3614 674.3534 12.0 1 22 0.99 +1Score > 43 indicates identity
Score > 35 indicates homology		 AMKGPR + Oxidation (M)
1359 363.1978 1086.5716 1086.5530 17.1 1 22 0.73 +1Score > 42 indicates identity
Score > 34 indicates homology		 AGRQGDIGASR
3286 696.3411 2086.0015 2085.9816 9.53 1 22 0.086 +1Score > 39 indicates identity
Score > 24 indicates homology		 ALDTYNEINAVMNLVLFE + 2 Deamidated (NQ); Oxidation (M)
701 397.2096 792.4046 792.4017 3.66 1 22 0.2 +1Score > 42 indicates identity
Score > 28 indicates homology		 FEQAGLK + Deamidated (NQ)
1646 606.3446 1210.6746 1210.6782 -2.93 0 22 0.94 +1Score > 38 indicates identity
Score > 35 indicates homology		 QISVRPNAAQK
2607 414.9812 1655.8957 1655.9246 -17.5 1 22 0.11 +1Score > 39 indicates identity
Score > 25 indicates homology		 RVAIYGAPDLVIGLAE
2774 581.2891 1740.8455 1740.8353 5.87 1 22 0.16 +1Score > 40 indicates identity
Score > 27 indicates homology		 YVCQAATSGLTEVSVR + Deamidated (NQ)
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