MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2630 834.4669 1666.9192 1666.9253 -3.65 1 3 0.73 +1Score > 37 indicates identity
Score > 15 indicates homology
KPLIATTNEISINPR + Deamidated (NQ)
3354 709.7238 2126.1496 2126.1591 -4.50 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
MLTAEMVKPGAIVIDVGINR
3754 475.4756 2372.3416 2372.3063 14.9 1 3 0.97 +1Score > 31 indicates identity
Score > 16 indicates homology
QVSTWLDNTQASIISIISRLK
3773 1194.0922 2386.1698 2386.1891 -8.05 1 3 0.79 +1Score > 39 indicates identity
Score > 15 indicates homology
DVVINAGGGIHGHPNGAQGGGKAFR + Deamidated (NQ)
2669 843.9456 1685.8766 1685.8835 -4.08 0 3 0.77 +1Score > 40 indicates identity
Score > 15 indicates homology
SNQLLSTAGLNLPSLR + 3 Deamidated (NQ)
1163 997.5164 996.5091 996.5240 -14.9 1 3 1 +1Score > 40 indicates identity
Score > 16 indicates homology
DKAVIHSVE
3543 744.0054 2228.9944 2228.9541 18.1 0 3 0.9 +1Score > 37 indicates identity
Score > 15 indicates homology
LFSFNAPMGACPACNGLGVTE + Deamidated (NQ); Oxidation (M)
3693 772.4266 2314.2580 2314.2355 9.71 0 3 1 +1Score > 34 indicates identity
Score > 16 indicates homology
IADLSLDFGKPVALGIAGPGMTR + Oxidation (M)
1803 638.8600 1275.7054 1275.6823 18.2 0 3 0.66 +1Score > 39 indicates identity
Score > 14 indicates homology
GLLNATGPITYR + Deamidated (NQ)
3662 768.3836 2302.1290 2302.1176 4.93 1 3 0.56 +1Score > 39 indicates identity
Score > 13 indicates homology
SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
4045 897.4619 2689.3639 2689.3303 12.5 1 3 0.81 +1Score > 37 indicates identity
Score > 15 indicates homology
LVDMGEAVGIIAAQSIGEPGTQLTMR + Deamidated (NQ); 2 Oxidation (M)
1653 607.3339 1212.6532 1212.6462 5.80 0 3 0.83 +1Score > 40 indicates identity
Score > 15 indicates homology
QISVRPNAAQK + 2 Deamidated (NQ)
3152 999.4949 1996.9752 1996.9850 -4.86 1 3 0.72 +1Score > 40 indicates identity
Score > 14 indicates homology
LTLITPAMSVGSKLDCFE + Oxidation (M)
3807 484.4525 2417.2261 2417.2664 -16.7 1 3 0.68 +1Score > 38 indicates identity
Score > 14 indicates homology
LEQILALSGCDYLTISPHFLK
4264 734.3870 2933.5189 2933.4999 6.47 0 3 0.81 +1Score > 35 indicates identity
Score > 15 indicates homology
VTPLQMLVVGIFEPIFYFLNMFIGE + Deamidated (NQ); Oxidation (M)
2921 457.4597 1825.8097 1825.8442 -18.9 1 3 0.82 +1Score > 38 indicates identity
Score > 15 indicates homology
AGLGNLGAPSSGQEEPNAR + 2 Deamidated (NQ)
4472 820.4213 3277.6561 3277.6839 -8.48 0 3 1 +1Score > 35 indicates identity
Score > 16 indicates homology
NLIMRPDQMYGNVVVTSIGQLVLYNAQLE
3199 675.0214 2022.0424 2022.0455 -1.57 0 3 0.78 +1Score > 39 indicates identity
Score > 15 indicates homology
LSALPDPPQDIVAAMQALR + Deamidated (NQ); Oxidation (M)
4388 788.4131 3149.6233 3149.6846 -19.5 1 3 0.52 +1Score > 35 indicates identity
Score > 13 indicates homology
DLPWPSVTGLGRAALAAGAAGLTVHPRPDQR
2182 489.6348 1465.8826 1465.8617 14.3 1 3 0.64 +1Score > 30 indicates identity
Score > 14 indicates homology
IIVVVGAPGDKGVSR
4008 659.8152 2635.2317 2635.2813 -18.8 1 3 0.88 +1Score > 37 indicates identity
Score > 15 indicates homology
RHHFTPGNQLLVVQNVMIDDAGR + 3 Deamidated (NQ); Oxidation (M)
3029 636.3438 1906.0096 1905.9731 19.1 0 3 0.97 +1Score > 38 indicates identity
Score > 16 indicates homology
HTNPCGVAVGPVVASALTR + Deamidated (NQ)
4034 892.7879 2675.3419 2675.3523 -3.90 1 3 0.82 +1Score > 37 indicates identity
Score > 15 indicates homology
IAMALTIAALYASGDSRIHNTACVR + Deamidated (NQ)
1579 591.3664 1180.7182 1180.7067 9.80 1 3 0.66 +1Score > 32 indicates identity
Score > 14 indicates homology
LNLPAVIAEIK + Deamidated (NQ)
3771 796.0912 2385.2518 2385.2362 6.52 1 3 0.88 +1Score > 37 indicates identity
Score > 15 indicates homology
MTEPVRVQVQTANPYPVIIGR + 2 Deamidated (NQ); Oxidation (M)
3820 606.5971 2422.3593 2422.3220 15.4 1 3 0.88 +1Score > 31 indicates identity
Score > 15 indicates homology
AFLIPLDSSPGIVLQDPRSQLR + Deamidated (NQ)
3823 809.7541 2426.2405 2426.2086 13.1 1 3 0.6 +1Score > 38 indicates identity
Score > 14 indicates homology
AKNVVGLSAMNFGTFPMVNGLSR + Deamidated (NQ); Oxidation (M)
3758 793.7691 2378.2855 2378.2416 18.4 1 3 0.69 +1Score > 34 indicates identity
Score > 14 indicates homology
AAGSIGYPLVVRPSYVLGGRAME + Oxidation (M)
2860 601.2747 1800.8023 1800.7762 14.5 1 3 0.66 +1Score > 38 indicates identity
Score > 14 indicates homology
NSQPNDGSEDSKPVQAE
4619 945.4990 3777.9669 3777.9207 12.2 1 3 0.68 +1Score > 32 indicates identity
Score > 14 indicates homology
TQRGILNANNINQLCAQLNVTNDALLQGLVPLASE + 3 Deamidated (NQ)
2998 628.3054 1881.8944 1881.9234 -15.4 1 3 0.64 +1Score > 40 indicates identity
Score > 14 indicates homology
ALAWRLDGQGPDNGAWR
3403 717.3549 2149.0429 2149.0724 -13.8 1 3 0.52 +1Score > 40 indicates identity
Score > 13 indicates homology
RLGVAFNQMADNINALIASK + 4 Deamidated (NQ)
3219 679.3713 2035.0921 2035.0738 8.97 1 3 0.61 +1Score > 37 indicates identity
Score > 14 indicates homology
FLNQLLHIVQHQKTVSE + 2 Deamidated (NQ)
2695 568.0023 1700.9851 1700.9535 18.6 1 3 1 +1Score > 32 indicates identity
Score > 16 indicates homology
IYPKNIIMIGPTGVGK + Deamidated (NQ)
2834 594.6817 1781.0233 1781.0312 -4.44 1 3 0.69 +1Score > 32 indicates identity
Score > 14 indicates homology
RLVNPQLSGLPAFLTR
4378 784.4025 3133.5809 3133.6408 -19.1 1 3 0.98 +1Score > 36 indicates identity
Score > 16 indicates homology
LGLPVVAVIDSNSDPRGITYPIPGNDDAIR
3310 1051.0125 2100.0104 2099.9769 16.0 0 3 0.99 +1Score > 39 indicates identity
Score > 16 indicates homology
MSTIAIVDYGMGNFHSVAR + 2 Oxidation (M)
4004 879.4154 2635.2244 2635.2622 -14.4 1 3 0.67 +1Score > 37 indicates identity
Score > 14 indicates homology
VVAIIGDGSLTGGMAFEAMNHLGDTK + 2 Oxidation (M)
4031 669.3256 2673.2733 2673.2956 -8.33 1 3 0.68 +1Score > 38 indicates identity
Score > 14 indicates homology
YPNTSDLDRLTQSHPILNMIDTK + 2 Deamidated (NQ)
2576 820.4288 1638.8430 1638.8287 8.76 1 3 0.73 +1Score > 40 indicates identity
Score > 14 indicates homology
RDDLSAIDFSLLMK + Oxidation (M)
3525 738.0312 2211.0718 2211.1034 -14.3 1 3 0.57 +1Score > 39 indicates identity
Score > 13 indicates homology
WNLAVIQLFSAEGMDTFIR + Deamidated (NQ)
3082 971.5444 1941.0742 1941.0836 -4.81 0 3 0.65 +1Score > 35 indicates identity
Score > 14 indicates homology
AIILVSLPFNASWLNQR
4342 1021.8823 3062.6251 3062.5780 15.4 0 3 1 +1Score > 33 indicates identity
Score > 16 indicates homology
LIPSGILHNNVTCLIGNGVVLSPSALMQE + Deamidated (NQ); Oxidation (M)
4748 887.4522 5318.6695 5318.6155 10.2 0 3 1 +1Score > 29 indicates identity
Score > 16 indicates homology
YPSYMNNNLIGLTAPLIPTPQLHFLMTGYTPLTSDSDIHTQQLVNVR + 3 Deamidated (NQ); 2 Oxidation (M)
2489 402.7330 1606.9029 1606.8943 5.32 1 3 0.64 +1Score > 35 indicates identity
Score > 14 indicates homology
FGVLPQPAPDAIRAR
3104 655.0449 1962.1129 1962.0759 18.9 1 3 1 +1Score > 32 indicates identity
Score > 16 indicates homology
LADPSNIVAGNQRVVRPR + Deamidated (NQ)
2391 782.4274 1562.8402 1562.8529 -8.08 0 3 0.99 +1Score > 39 indicates identity
Score > 16 indicates homology
TLGALVHNQQVLNR + Deamidated (NQ)
3981 866.7800 2597.3182 2597.2717 17.9 0 3 0.78 +1Score > 37 indicates identity
Score > 15 indicates homology
ILQHMDQAIGLNTSNLQMLVLDE + 2 Deamidated (NQ)
4251 974.4820 2920.4242 2920.4448 -7.06 0 3 0.83 +1Score > 37 indicates identity
Score > 15 indicates homology
NSQALISGGAMGGTVSAVASLVDLAASSDVR + Deamidated (NQ); Oxidation (M)
4751 778.9738 5445.7657 5445.8687 -18.9 1 3 0.57 +1Score > 28 indicates identity
Score > 13 indicates homology
ILTNFAGPMNNFILGFILFTLAVFLQGGVTDLNTNQIGQVIPNGPAAEAGLK + Deamidated (NQ); Oxidation (M)
3848 614.0760 2452.2749 2452.2922 -7.04 1 3 0.57 +1Score > 37 indicates identity
Score > 13 indicates homology
AVDRIIQDPQGNTIVTGGIISQR + 2 Deamidated (NQ)
2617 555.2987 1662.8743 1662.9053 -18.6 1 3 0.85 +1Score > 39 indicates identity
Score > 15 indicates homology
NKNIAISHNPISNLK + Deamidated (NQ)
3281 695.6945 2084.0617 2084.1014 -19.1 1 3 0.71 +1Score > 39 indicates identity
Score > 14 indicates homology
RLIQQDTALVNLTQWQR + 2 Deamidated (NQ)
2120 475.5778 1423.7116 1423.6878 16.7 0 3 0.71 +1Score > 41 indicates identity
Score > 14 indicates homology
NSLINPCHNVVR + 2 Deamidated (NQ)
4406 795.4135 3177.6249 3177.6104 4.58 1 3 1 +1Score > 35 indicates identity
Score > 16 indicates homology
HPMTDLDRQMIDWFAPSGKPIHVLLTK + 2 Oxidation (M)
4044 897.4592 2689.3558 2689.3204 13.2 1 3 0.98 +1Score > 37 indicates identity
Score > 15 indicates homology
ASPNIMNGLQNDFKVLVIGAGGLGCE + Oxidation (M)
2154 482.6028 1444.7866 1444.7674 13.3 1 3 0.64 +1Score > 39 indicates identity
Score > 14 indicates homology
TANKIIAHNTFSK + Deamidated (NQ)
2605 828.4852 1654.9558 1654.9406 9.20 1 3 1 +1Score > 33 indicates identity
Score > 16 indicates homology
LNHSVLLVGYGQVKK + Deamidated (NQ)
4089 683.5802 2730.2917 2730.3170 -9.28 1 3 0.76 +1Score > 38 indicates identity
Score > 14 indicates homology
WICSAQQVAVSTSSNPAPISEIDIR + 2 Deamidated (NQ)
3850 820.4369 2458.2889 2458.2632 10.5 1 3 0.72 +1Score > 37 indicates identity
Score > 14 indicates homology
GIALGQLDPFTQLPFQAESVTPK + 2 Deamidated (NQ)
3712 777.7292 2330.1658 2330.1981 -13.9 0 3 1 +1Score > 38 indicates identity
Score > 15 indicates homology
QMVAFIGAVPGIGPSLQKPFQE + Deamidated (NQ); Oxidation (M)
4215 959.8393 2876.4961 2876.5405 -15.4 1 3 0.83 +1Score > 35 indicates identity
Score > 15 indicates homology
VTVMNTLTVNLHLLMVSFYRPSGKR + Deamidated (NQ)
3549 744.7436 2231.2090 2231.2195 -4.71 1 3 0.6 +1Score > 35 indicates identity
Score > 13 indicates homology
LSANLALLSDVSMAVLGGSLKR + Deamidated (NQ); Oxidation (M)
4292 997.5359 2989.5859 2989.5814 1.49 1 3 0.54 +1Score > 34 indicates identity
Score > 13 indicates homology
FDLNVSKSAWLTAIPLAQQFWQTVAR
4593 918.7632 3671.0237 3670.9579 17.9 0 3 1 +1Score > 28 indicates identity
Score > 15 indicates homology
IQTVCLGSLPLMTPQGTFVINGVSWTIVNQILR + Oxidation (M)
3858 823.4339 2467.2799 2467.2814 -0.63 1 3 0.54 +1Score > 37 indicates identity
Score > 13 indicates homology
VSIVGAILNMPGQNMIPAIKDPR + 2 Deamidated (NQ); 2 Oxidation (M)
3964 516.2693 2576.3101 2576.3082 0.73 1 3 0.55 +1Score > 37 indicates identity
Score > 13 indicates homology
NVDPVGVHTGDSITVAPALTLTDRE
4572 893.9462 3571.7557 3571.7100 12.8 1 3 0.66 +1Score > 34 indicates identity
Score > 14 indicates homology
LANGTVTLKDMNGAHDQQTVAAADLTNTLQQWK + 3 Deamidated (NQ); Oxidation (M)
2609 553.2863 1656.8371 1656.8683 -18.8 0 3 0.64 +1Score > 40 indicates identity
Score > 13 indicates homology
GNPTIQASVILSDNTK
3235 681.0298 2040.0676 2040.0310 17.9 1 3 1 +1Score > 38 indicates identity
Score > 15 indicates homology
VNVDNRFATVTLSATTGMK + Oxidation (M)
3637 574.0620 2292.2189 2292.1784 17.7 1 3 1 +1Score > 36 indicates identity
Score > 15 indicates homology
AQALFASAVVCGALTTEAVLGSR + Deamidated (NQ)
3941 851.1229 2550.3469 2550.2967 19.7 1 3 0.85 +1Score > 36 indicates identity
Score > 15 indicates homology
NSGISGRPLMLLATALKMNMNLR + 2 Deamidated (NQ); 3 Oxidation (M)
2954 928.4940 1854.9734 1854.9404 17.8 1 3 0.6 +1Score > 38 indicates identity
Score > 13 indicates homology
EFLTQFLQVVFDQIE
3940 851.1219 2550.3439 2550.3006 17.0 0 3 0.55 +1Score > 36 indicates identity
Score > 13 indicates homology
TGNQWKPLTGGIFPLDLLHQIAE + 3 Deamidated (NQ)
3149 665.3139 1992.9199 1992.9472 -13.7 0 3 0.99 +1Score > 39 indicates identity
Score > 15 indicates homology
GDFIPGIMVDGMDVLCVR
4566 893.4594 3569.8085 3569.8124 -1.10 0 3 1 +1Score > 34 indicates identity
Score > 15 indicates homology
MHQFQLIGLMLSTLINNLTNFYTLFHMLAK + 2 Deamidated (NQ); Oxidation (M)
2288 505.2812 1512.8218 1512.8035 12.1 0 3 0.82 +1Score > 39 indicates identity
Score > 15 indicates homology
SSTPSNQNIIPIIK + 2 Deamidated (NQ)
4200 956.4810 2866.4212 2866.4106 3.70 1 3 1 +1Score > 37 indicates identity
Score > 15 indicates homology
FSILSKPGGQPCLILSGNGNQAKCYR + 2 Deamidated (NQ)
4325 759.4031 3033.5833 3033.5303 17.5 1 3 0.99 +1Score > 35 indicates identity
Score > 15 indicates homology
RPALTPIENPTTQPGYKPVQMPMPKPE + Deamidated (NQ); Oxidation (M)
4601 921.9794 3683.8885 3683.8928 -1.16 1 3 1 +1Score > 33 indicates identity
Score > 15 indicates homology
MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSR + Deamidated (NQ)
3623 760.0574 2277.1504 2277.1324 7.89 1 3 0.87 +1Score > 39 indicates identity
Score > 15 indicates homology
NPQSVNIHCLKAHSPLASFR + 2 Deamidated (NQ)
258 517.3025 516.2952 516.2908 8.65 0 3 0.96 +1Score > 48 indicates identity
Score > 15 indicates homology
VSSPK
3619 759.3483 2275.0231 2275.0024 9.09 1 3 0.73 +1Score > 37 indicates identity
Score > 14 indicates homology
LILSNQYYLMSQMDPENAE + Deamidated (NQ); Oxidation (M)
4412 1061.5261 3181.5565 3181.5682 -3.68 1 3 1 +1Score > 36 indicates identity
Score > 15 indicates homology
LAMHGTHNMLNSLAAIGVATQMGIADDKIR + Deamidated (NQ); 2 Oxidation (M)
733 405.7281 809.4416 809.4395 2.60 1 3 0.99 +1Score > 38 indicates identity
Score > 15 indicates homology
TSTKAFR
3601 754.4051 2260.1935 2260.1699 10.4 1 3 0.82 +1Score > 37 indicates identity
Score > 15 indicates homology
VVLSASDVLDYGRGDLLTDPR
3855 494.2692 2466.3096 2466.2950 5.94 1 3 0.88 +1Score > 36 indicates identity
Score > 15 indicates homology
GCMLIVTCVAILAVDFRLFPR + Oxidation (M)
3346 707.3699 2119.0879 2119.0507 17.5 1 3 0.72 +1Score > 39 indicates identity
Score > 14 indicates homology
ISANDFLGSLELQLPDMVR + 2 Deamidated (NQ)
4682 1020.5323 4078.1001 4078.0986 0.36 1 3 1 +1Score > 32 indicates identity
Score > 15 indicates homology
IFGDSITPGSTRLGAIIVYLVGFWLLDVGNNATQGPCR + Deamidated (NQ)
3927 846.4364 2536.2874 2536.2843 1.22 1 3 0.78 +1Score > 37 indicates identity
Score > 14 indicates homology
VQPVAMNLVPTIQKDIQQQPNR + 4 Deamidated (NQ); Oxidation (M)
3203 1012.5303 2023.0460 2023.0109 17.4 1 3 0.91 +1Score > 39 indicates identity
Score > 15 indicates homology
DLLQENGTPIVDPINDIR + 2 Deamidated (NQ)
3890 834.0759 2499.2059 2499.2176 -4.70 0 3 1 +1Score > 39 indicates identity
Score > 15 indicates homology
GGCLNLSQSNVFLDLVQGANHQK + Deamidated (NQ)
4275 986.5377 2956.5913 2956.5408 17.1 0 3 0.78 +1Score > 33 indicates identity
Score > 14 indicates homology
YAAYGLVMPSLVLNYFGQGALLIQNPK + Deamidated (NQ); Oxidation (M)
2502 805.9844 1609.9542 1609.9589 -2.90 0 3 0.83 +1Score > 31 indicates identity
Score > 14 indicates homology
VLVMTPQILLQSLR
4294 998.4963 2992.4671 2992.4158 17.1 1 3 0.61 +1Score > 37 indicates identity
Score > 13 indicates homology
HQSPTDLSLMALGNCVTHLLQNKVPAE + 4 Deamidated (NQ); Oxidation (M)
2729 860.4286 1718.8426 1718.8257 9.83 1 3 0.98 +1Score > 41 indicates identity
Score > 15 indicates homology
QPSMGITAAGSKGGASGGGK + Deamidated (NQ)
3565 747.0403 2238.0991 2238.1175 -8.23 1 3 0.88 +1Score > 39 indicates identity
Score > 15 indicates homology
AGGNILIMGTLKGNAHAGSQGDR + Deamidated (NQ)
4040 672.0694 2684.2485 2684.2210 10.2 1 3 0.71 +1Score > 37 indicates identity
Score > 14 indicates homology
AIINFASENGLQSWQQCDVTMVR + 2 Deamidated (NQ); Oxidation (M)
3197 1012.0268 2022.0390 2022.0455 -3.21 0 3 0.83 +1Score > 39 indicates identity
Score > 14 indicates homology
LSALPDPPQDIVAAMQALR + Deamidated (NQ); Oxidation (M)
3591 751.4086 2251.2040 2251.1631 18.2 1 3 0.61 +1Score > 36 indicates identity
Score > 13 indicates homology
SVAMTIADGVALSNTGRGYVIR + Deamidated (NQ)
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Not what you expected? Try the select summary.