MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3579 562.2777 2245.0817 2245.1083 -11.8 1 4 0.79 +1Score > 39 indicates identity
Score > 16 indicates homology
VRCLPVDPQIVSCSTDATVK + Deamidated (NQ)
4000 878.4557 2632.3453 2632.3095 13.6 1 4 0.96 +1Score > 37 indicates identity
Score > 16 indicates homology
TVILDGITYGGFNVVDIQMLYRE + Deamidated (NQ); Oxidation (M)
3479 731.3835 2191.1287 2191.1094 8.81 1 4 0.96 +1Score > 38 indicates identity
Score > 16 indicates homology
AQLGAANAGVRPGGRAIQPDDR + 2 Deamidated (NQ)
3666 768.4297 2302.2673 2302.3096 -18.4 1 4 0.77 +1Score > 33 indicates identity
Score > 15 indicates homology
LSVLQHAPWLGRLLCASILR
4402 792.4300 3165.6909 3165.6930 -0.67 1 4 1 +1Score > 32 indicates identity
Score > 17 indicates homology
EAILNGCLTALQMGGPILGFPLHSTLVTVK + Oxidation (M)
3345 1060.5491 2119.0836 2119.1030 -9.14 1 4 0.49 +1Score > 39 indicates identity
Score > 13 indicates homology
RGALQAIDQMSLFRPLCK + Oxidation (M)
2619 833.4946 1664.9746 1665.0076 -19.8 0 4 0.43 +1Score > 31 indicates identity
Score > 13 indicates homology
SLPQAVLSAIVIVNLK + Deamidated (NQ)
3044 959.0111 1916.0076 1915.9931 7.60 0 4 0.94 +1Score > 38 indicates identity
Score > 16 indicates homology
NAINLDFLSIYIVYTR + 2 Deamidated (NQ)
3468 1093.6010 2185.1874 2185.1711 7.48 1 4 0.71 +1Score > 35 indicates identity
Score > 15 indicates homology
VMAILCLASDLADLQARLGR
2686 566.6355 1696.8847 1696.8858 -0.68 1 4 0.9 +1Score > 39 indicates identity
Score > 16 indicates homology
FGALLIIDEVMTGFR + Oxidation (M)
2980 623.3774 1867.1104 1867.0971 7.10 0 4 0.67 +1Score > 27 indicates identity
Score > 15 indicates homology
GVISFYLIGQLLYLIR
3292 696.7091 2087.1055 2087.1190 -6.51 1 4 0.6 +1Score > 37 indicates identity
Score > 14 indicates homology
IAQLVFVPVVQAEFNIVAE + 2 Deamidated (NQ)
4162 703.1447 2808.5497 2808.5064 15.4 1 4 0.87 +1Score > 31 indicates identity
Score > 16 indicates homology
SLSLMVDNVKQLPLTMDAMILALHR
4249 973.8682 2918.5828 2918.5324 17.3 1 4 1 +1Score > 33 indicates identity
Score > 17 indicates homology
AINHAMAAGVPIVFAINKVDKPTANPDK + Deamidated (NQ); Oxidation (M)
4309 1004.8832 3011.6278 3011.5750 17.5 1 4 1 +1Score > 32 indicates identity
Score > 16 indicates homology
LLNQGGLFVLTGASAALNRTSGMIAYGATK + Deamidated (NQ); Oxidation (M)
2392 782.4288 1562.8430 1562.8668 -15.2 1 4 0.89 +1Score > 39 indicates identity
Score > 16 indicates homology
KTGAAALASPATYTIK
2036 463.9351 1388.7835 1388.7888 -3.84 1 4 0.79 +1Score > 37 indicates identity
Score > 15 indicates homology
AVRAATYGGALALR
3418 718.7411 2153.2015 2153.2055 -1.89 1 4 0.51 +1Score > 33 indicates identity
Score > 14 indicates homology
LLNGLLTAAGADSAGQKLAVLR + 2 Deamidated (NQ)
4060 676.3539 2701.3865 2701.3671 7.17 1 4 0.53 +1Score > 36 indicates identity
Score > 14 indicates homology
IPSSTTLSPPRHDSLSLDGGTVNPPR + Deamidated (NQ)
2849 895.9021 1789.7896 1789.7907 -0.61 1 4 0.66 +1Score > 38 indicates identity
Score > 15 indicates homology
NRNFSTTGNFGFGIQE + 2 Deamidated (NQ)
4767 1165.6238 5823.0826 5823.0366 7.90 1 4 1 +1Score > 25 indicates identity
Score > 16 indicates homology
SPVVDSAVATLSSPSIAVFGGVSFKTNGGLPSGMPLTSILNSLNHCLLVGSAIIQVLE + 2 Deamidated (NQ)
976 463.2254 924.4362 924.4521 -17.2 1 4 0.9 +1Score > 40 indicates identity
Score > 16 indicates homology
MVKTMQR + 2 Oxidation (M)
3072 967.9469 1933.8792 1933.9091 -15.4 1 4 0.6 +1Score > 39 indicates identity
Score > 14 indicates homology
GTPFAAQIAAEQAAQIAME + Deamidated (NQ); Oxidation (M)
3375 1068.6079 2135.2012 2135.1990 1.04 0 4 0.8 +1Score > 32 indicates identity
Score > 15 indicates homology
VTLLEPQADGSWAGLLKPLK
3757 475.4765 2372.3461 2372.3063 16.8 1 4 0.81 +1Score > 30 indicates identity
Score > 15 indicates homology
QVSTWLDNTQASIISIISRLK
3655 576.0738 2300.2661 2300.2780 -5.18 1 4 0.67 +1Score > 33 indicates identity
Score > 15 indicates homology
LSAIRAVLPQSAVLAFPFTVAE + Deamidated (NQ)
3569 1122.1135 2242.2124 2242.2494 -16.5 0 4 0.94 +1Score > 35 indicates identity
Score > 16 indicates homology
MLLILLSVALLALSSAQNLNE + Deamidated (NQ); Oxidation (M)
2544 813.8995 1625.7844 1625.7910 -4.05 0 4 0.78 +1Score > 40 indicates identity
Score > 15 indicates homology
AHNPPTGTPGDLGAGHK
2897 454.2393 1812.9281 1812.8928 19.5 0 4 0.74 +1Score > 40 indicates identity
Score > 15 indicates homology
QYAITSDIMDTVIATR + Oxidation (M)
3148 664.6874 1991.0404 1991.0146 13.0 0 4 0.67 +1Score > 39 indicates identity
Score > 15 indicates homology
YATNQSLPLILVCASGGAR + Deamidated (NQ)
4441 806.6761 3222.6753 3222.6217 16.6 1 4 0.62 +1Score > 34 indicates identity
Score > 14 indicates homology
QVGVVLQDNVLLNRSIIDNIALADPGMPVE + 5 Deamidated (NQ); Oxidation (M)
2581 820.9624 1639.9102 1639.8907 11.9 1 4 0.73 +1Score > 37 indicates identity
Score > 15 indicates homology
EPLVVAAKHHVNGNR
3992 874.4415 2620.3027 2620.2841 7.10 1 4 0.47 +1Score > 38 indicates identity
Score > 13 indicates homology
QRLAQLQQQAGAAAGAPNGAPNGTTLN + 3 Deamidated (NQ)
4183 946.7402 2837.1988 2837.1933 1.93 1 4 0.66 +1Score > 32 indicates identity
Score > 15 indicates homology
MSNWLHVVNNNNSNNNNKTSDGITK + 7 Deamidated (NQ); Oxidation (M)
3317 1055.5580 2109.1014 2109.0702 14.8 1 4 0.86 +1Score > 38 indicates identity
Score > 16 indicates homology
TQGNQKHLLIDTASQVNLE + Deamidated (NQ)
4227 965.1918 2892.5536 2892.5960 -14.7 1 4 0.53 +1Score > 33 indicates identity
Score > 14 indicates homology
LAIGIDLGTTNSLIAVWKDGAAQLIPNK + Deamidated (NQ)
203 459.2656 458.2583 458.2601 -3.95 0 4 0.8 +1Score > 39 indicates identity
Score > 15 indicates homology
GGGIR
3413 718.3868 2152.1386 2152.1310 3.53 1 4 0.88 +1Score > 37 indicates identity
Score > 16 indicates homology
QALNSLAQMEPALVRQLPR + 2 Deamidated (NQ); Oxidation (M)
3949 852.4378 2554.2916 2554.2407 19.9 0 4 0.75 +1Score > 38 indicates identity
Score > 15 indicates homology
MNISSQNVLLPSPIPSSSPMASHK + Deamidated (NQ); 2 Oxidation (M)
2222 741.9474 1481.8802 1481.8817 -0.99 1 4 0.8 +1Score > 32 indicates identity
Score > 15 indicates homology
KQATAIVLLGVIGAE
3475 1095.0715 2188.1284 2188.1232 2.42 1 4 0.7 +1Score > 38 indicates identity
Score > 15 indicates homology
TVLMSPLMVAAAAVAGEIADAR + 2 Oxidation (M)
2754 865.4777 1728.9408 1728.9523 -6.62 1 4 0.89 +1Score > 38 indicates identity
Score > 16 indicates homology
GAGVPFRVLGTVGGDSLK
2597 550.9861 1649.9365 1649.9114 15.2 1 4 0.48 +1Score > 34 indicates identity
Score > 13 indicates homology
SVAAFGGHGVVIPQRR
3381 714.3851 2140.1335 2140.0987 16.3 1 4 0.78 +1Score > 37 indicates identity
Score > 15 indicates homology
PLLDSFTVDHKIMNAPAVR + Deamidated (NQ); Oxidation (M)
2318 765.4326 1528.8506 1528.8348 10.3 0 4 0.69 +1Score > 37 indicates identity
Score > 15 indicates homology
TSLVNLLGGLTTPDK + Deamidated (NQ)
3450 726.6934 2177.0584 2177.0423 7.38 1 4 0.85 +1Score > 39 indicates identity
Score > 16 indicates homology
ILGGEYQPDSGGVLVDGQAMR + Oxidation (M)
2886 603.3384 1806.9934 1806.9628 16.9 1 4 0.83 +1Score > 36 indicates identity
Score > 15 indicates homology
FALIGDNGRSALFANLK + Deamidated (NQ)
3426 1083.5356 2165.0566 2165.0721 -7.15 1 4 0.89 +1Score > 39 indicates identity
Score > 16 indicates homology
VVSDFRINISNQMATMPAR + Oxidation (M)
3684 770.7726 2309.2960 2309.2703 11.1 1 4 0.79 +1Score > 31 indicates identity
Score > 15 indicates homology
QTRLIAGLVSGLDANIQVGGGIR + 2 Deamidated (NQ)
3755 594.0935 2372.3449 2372.3146 12.8 0 4 0.83 +1Score > 30 indicates identity
Score > 15 indicates homology
VWLCICSIGILLMVLVGGITR
3079 647.0388 1938.0946 1938.0608 17.4 0 4 0.78 +1Score > 33 indicates identity
Score > 15 indicates homology
DAIIVGCAQVLALVPGVSR + Deamidated (NQ)
4172 940.7980 2819.3722 2819.3324 14.1 0 4 0.85 +1Score > 37 indicates identity
Score > 16 indicates homology
AQVSVDMFLFSSQYQDVASLSNLPR + 2 Deamidated (NQ); Oxidation (M)
4454 1080.5210 3238.5412 3238.5641 -7.07 1 4 1 +1Score > 36 indicates identity
Score > 16 indicates homology
VLLATMNMQTIAQMSCNPAMGGVAKGQIVR + Deamidated (NQ); 3 Oxidation (M)
1218 1016.5492 1015.5419 1015.5437 -1.78 0 4 1 +1Score > 42 indicates identity
Score > 16 indicates homology
ILLLDSVDE
3830 813.1123 2436.3151 2436.3125 1.07 0 4 0.5 +1Score > 35 indicates identity
Score > 13 indicates homology
ISQSNAISLLSHLWVANTAAALR + Deamidated (NQ)
2692 850.9808 1699.9470 1699.9290 10.6 1 4 0.89 +1Score > 36 indicates identity
Score > 16 indicates homology
QAIQIIVKMAAANGVR + 2 Deamidated (NQ); Oxidation (M)
3867 826.1046 2475.2920 2475.3043 -4.98 1 4 0.95 +1Score > 37 indicates identity
Score > 16 indicates homology
GQTLLKNVPILSDVFTMNNVVR + 2 Deamidated (NQ); Oxidation (M)
393 634.2952 633.2879 633.2758 19.1 0 4 0.68 +1Score > 41 indicates identity
Score > 15 indicates homology
QWAQT + Deamidated (NQ)
2280 378.7177 1510.8417 1510.8177 15.9 0 4 0.9 +1Score > 37 indicates identity
Score > 16 indicates homology
MNLAVAAALPSVTPR + Deamidated (NQ)
2907 456.2391 1820.9273 1820.9528 -14.0 1 4 0.88 +1Score > 40 indicates identity
Score > 16 indicates homology
GIFGTCAGMILLAKQLE
3404 717.3858 2149.1356 2149.1419 -2.95 1 4 0.46 +1Score > 37 indicates identity
Score > 13 indicates homology
VSIIGISNDLKFTDFLDPR
3928 846.4492 2536.3258 2536.2843 16.4 1 4 0.79 +1Score > 36 indicates identity
Score > 15 indicates homology
VQPVAMNLVPTIQKDIQQQPNR + 4 Deamidated (NQ); Oxidation (M)
4056 898.4557 2692.3453 2692.2915 20.0 0 4 0.68 +1Score > 37 indicates identity
Score > 15 indicates homology
GPPGPAGPAGLMGPPGLQGPSGLPGDPGDR + Deamidated (NQ); Oxidation (M)
4201 956.4839 2866.4299 2866.4133 5.80 0 4 0.6 +1Score > 37 indicates identity
Score > 14 indicates homology
MIDLFQLTCLTSTVSISNFILYNR + 2 Deamidated (NQ); Oxidation (M)
4604 618.0015 3701.9653 3702.0131 -12.9 0 4 1 +1Score > 31 indicates identity
Score > 16 indicates homology
LSSSEPSTPAQTPLAAPIIPTPVLTSPGAVPPLPSPSK
4023 666.8469 2663.3585 2663.3411 6.53 0 4 0.73 +1Score > 37 indicates identity
Score > 15 indicates homology
AIQTLVTAMHLVQTNHQIVQMAAE + Deamidated (NQ); Oxidation (M)
3805 806.4172 2416.2298 2416.2234 2.64 1 4 0.86 +1Score > 38 indicates identity
Score > 15 indicates homology
DVYGNIEGVSVGLQVLNQQLNR + 2 Deamidated (NQ)
3120 658.3734 1972.0984 1972.1132 -7.51 1 4 0.61 +1Score > 35 indicates identity
Score > 14 indicates homology
IILVILDAISNIFQAAEK + 2 Deamidated (NQ)
3602 755.7245 2264.1517 2264.1397 5.30 0 4 0.96 +1Score > 38 indicates identity
Score > 16 indicates homology
VDAIVNAANSSLLGGGGVDGAIHR + 2 Deamidated (NQ)
4079 907.8209 2720.4409 2720.4021 14.2 1 4 0.58 +1Score > 35 indicates identity
Score > 14 indicates homology
AIDFSSNDVLVGIAASGRTPYVIGGLE
2976 623.3406 1867.0000 1867.0051 -2.73 0 4 0.82 +1Score > 37 indicates identity
Score > 15 indicates homology
IQVTGSPAVLQNALVQAR + 3 Deamidated (NQ)
3915 842.0907 2523.2503 2523.2501 0.059 1 4 0.69 +1Score > 38 indicates identity
Score > 14 indicates homology
IMSAADVANQYVDRMKPWVLAK + 2 Deamidated (NQ); Oxidation (M)
4226 724.1239 2892.4665 2892.4981 -10.9 1 4 0.87 +1Score > 36 indicates identity
Score > 15 indicates homology
DIVASTHKNGVIDNIGGFSAHIAIDVTK + Deamidated (NQ)
3566 747.0483 2238.1231 2238.1015 9.62 1 4 0.94 +1Score > 39 indicates identity
Score > 16 indicates homology
VDNLIKDINSYTNSTSVINE
3793 803.3982 2407.1728 2407.1577 6.27 0 4 0.74 +1Score > 39 indicates identity
Score > 15 indicates homology
NQAVNIINNMAPGDVFQTLTTK + 3 Deamidated (NQ); Oxidation (M)
3878 828.1135 2481.3187 2481.3075 4.51 1 4 0.93 +1Score > 35 indicates identity
Score > 16 indicates homology
QARSPPTSASVSTVIDGPTQILVR + 2 Deamidated (NQ)
4262 978.8424 2933.5054 2933.4732 11.0 1 4 0.94 +1Score > 36 indicates identity
Score > 16 indicates homology
LAQGLVGQPLLMPVAVPVDDNWNEVIE + 2 Deamidated (NQ); Oxidation (M)
4299 999.5282 2995.5628 2995.5073 18.5 1 4 0.51 +1Score > 35 indicates identity
Score > 13 indicates homology
HTAVIPIAGDQVTNDIAMALRTPTQYAE
2997 628.0073 1881.0001 1881.0068 -3.58 1 4 0.94 +1Score > 38 indicates identity
Score > 16 indicates homology
LVTPGIRPANSATGDQKR + Deamidated (NQ)
3304 699.3752 2095.1038 2095.0990 2.28 1 4 1 +1Score > 38 indicates identity
Score > 16 indicates homology
WTEFLNLLGDTVVLNSFK
4050 898.4448 2692.3126 2692.3312 -6.92 1 4 0.64 +1Score > 38 indicates identity
Score > 14 indicates homology
NPIITMINSGARGNISNYVQLAGMR + 3 Deamidated (NQ)
2298 757.9534 1513.8922 1513.9079 -10.4 1 4 1 +1Score > 31 indicates identity
Score > 16 indicates homology
IIVGTLINVGTGKTK + Deamidated (NQ)
4255 976.5305 2926.5697 2926.6254 -19.0 1 4 1 +1Score > 33 indicates identity
Score > 16 indicates homology
ARIAGVNIPNHQHTVIGLTAIFGIGTTR
3547 744.3990 2230.1752 2230.1814 -2.78 1 4 0.79 +1Score > 37 indicates identity
Score > 15 indicates homology
LTGMAMRVPTANVSVVDLTVR + Deamidated (NQ)
3704 775.4247 2323.2523 2323.2172 15.1 0 4 0.89 +1Score > 35 indicates identity
Score > 16 indicates homology
IVQDFVSNHVSNAPSLAAALIR + 2 Deamidated (NQ)
4148 933.8179 2798.4319 2798.4378 -2.12 1 4 1 +1Score > 37 indicates identity
Score > 16 indicates homology
SLQPLGLYAANSIFVGDYLTTLGQEK + Deamidated (NQ)
4546 859.4349 3433.7105 3433.7036 2.00 1 4 0.57 +1Score > 35 indicates identity
Score > 14 indicates homology
LEPMLNNALNEIKPQPDLSTLSMLFFSIPE + Deamidated (NQ); 2 Oxidation (M)
3146 664.3527 1990.0363 1990.0173 9.52 0 3 0.96 +1Score > 39 indicates identity
Score > 16 indicates homology
ISQLPPSTHYGHLGWAAR
3474 1095.0706 2188.1266 2188.1232 1.60 1 3 0.73 +1Score > 38 indicates identity
Score > 15 indicates homology
TVLMSPLMVAAAAVAGEIADAR + 2 Oxidation (M)
3980 648.8088 2591.2061 2591.1722 13.1 0 3 0.49 +1Score > 38 indicates identity
Score > 13 indicates homology
SLLSTSSLVQPQQQSQQSQQQQE + 5 Deamidated (NQ)
4233 967.8465 2900.5177 2900.4919 8.88 1 3 1 +1Score > 35 indicates identity
Score > 16 indicates homology
AGADYLVLGRPIYAADDPKAAAQAIIQE + Deamidated (NQ)
3454 546.2834 2181.1045 2181.1277 -10.6 1 3 1 +1Score > 39 indicates identity
Score > 16 indicates homology
LTNLGLVTVSLASGLGQHRDE + 2 Deamidated (NQ)
4001 878.4572 2632.3498 2632.3306 7.28 1 3 0.72 +1Score > 37 indicates identity
Score > 15 indicates homology
VQSYLTSTSSTFKLADTVILICGE
2086 705.9489 1409.8832 1409.8606 16.1 1 3 1 +1Score > 25 indicates identity
Score > 16 indicates homology
KLLPATVVNQVTK
3213 678.3405 2031.9997 2031.9605 19.3 1 3 0.98 +1Score > 39 indicates identity
Score > 16 indicates homology
SAQLVESMLQHMSDALVR + 2 Deamidated (NQ); Oxidation (M)
3507 735.3595 2203.0567 2203.0579 -0.57 0 3 0.76 +1Score > 39 indicates identity
Score > 15 indicates homology
MYYSTAVTGNIVNSVNVQSR + Deamidated (NQ)
3770 796.0579 2385.1519 2385.1634 -4.85 0 3 0.59 +1Score > 39 indicates identity
Score > 14 indicates homology
IISDVNSFMQNVLTNLYQNR + Deamidated (NQ); Oxidation (M)
4049 898.4421 2692.3045 2692.2634 15.3 1 3 0.48 +1Score > 38 indicates identity
Score > 13 indicates homology
NRNMWIVLSCVLYMIYICNGR + 2 Deamidated (NQ); Oxidation (M)
3715 585.0394 2336.1285 2336.1179 4.53 1 3 0.5 +1Score > 39 indicates identity
Score > 13 indicates homology
NASKGPHSPQVPSQVQSPMTSR + Deamidated (NQ); Oxidation (M)
2549 814.3964 1626.7782 1626.7997 -13.2 1 3 0.88 +1Score > 40 indicates identity
Score > 15 indicates homology
SIYDIQKVLAAVCM + Deamidated (NQ); Oxidation (M)
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Not what you expected? Try the select summary.