MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1615 598.3472 1194.6798 1194.6608 15.9 0 5 0.51 +1Score > 36 indicates identity
Score > 14 indicates homology
LVGAAAVLNAAPE
3448 726.3925 2176.1557 2176.1310 11.3 0 5 0.88 +1Score > 37 indicates identity
Score > 17 indicates homology
ATAIAPGTVVTVSNLFASCAAR
3533 741.3715 2221.0927 2221.1307 -17.1 1 5 1 +1Score > 39 indicates identity
Score > 17 indicates homology
KQPFVDTLFPPTNQSLFLE + Deamidated (NQ)
4352 771.6467 3082.5577 3082.5036 17.6 1 5 1 +1Score > 36 indicates identity
Score > 17 indicates homology
NIELVNQIINDYGADPDTWLPIFHQR + 2 Deamidated (NQ)
3280 1042.0455 2082.0764 2082.0932 -8.02 1 5 0.57 +1Score > 38 indicates identity
Score > 15 indicates homology
AFASLGPALRHQMLDLVNK + 2 Deamidated (NQ)
4680 814.8051 4068.9891 4068.9531 8.85 1 5 0.37 +1Score > 33 indicates identity
Score > 13 indicates homology
CGMIYMEPQMLGWRPLMVSWINTLPQSVSIIQKE + 2 Deamidated (NQ); 2 Oxidation (M)
2736 861.4676 1720.9206 1720.9148 3.39 0 5 0.6 +1Score > 38 indicates identity
Score > 15 indicates homology
SPTIAPLADPDWVAIR
3548 1116.5986 2231.1826 2231.1923 -4.33 1 5 0.64 +1Score > 37 indicates identity
Score > 15 indicates homology
GEPVRPALLWNDVRSAPQAR
3942 851.1242 2550.3508 2550.3006 19.7 0 5 0.93 +1Score > 35 indicates identity
Score > 17 indicates homology
TGNQWKPLTGGIFPLDLLHQIAE + 3 Deamidated (NQ)
2694 567.9664 1700.8774 1700.8958 -10.9 0 5 0.9 +1Score > 40 indicates identity
Score > 17 indicates homology
TNAVHGDKPSAPVGPVR
2867 901.5004 1800.9862 1800.9768 5.26 1 5 0.92 +1Score > 37 indicates identity
Score > 17 indicates homology
VIVGLPRMLDGSAGSSVK + Oxidation (M)
3963 857.4575 2569.3507 2569.3486 0.79 1 5 0.77 +1Score > 36 indicates identity
Score > 16 indicates homology
ALDDSVIDALSDDLNTPKALTLLR + Deamidated (NQ)
3911 631.5928 2522.3421 2522.3228 7.65 1 5 0.61 +1Score > 35 indicates identity
Score > 15 indicates homology
STKLEPIYNTAVTSTVGLTSPSTR
161 418.2314 417.2241 417.2223 4.29 0 5 0.87 +1Score > 43 indicates identity
Score > 17 indicates homology
AAVAS
3607 756.3901 2266.1485 2266.1118 16.2 1 5 0.85 +1Score > 39 indicates identity
Score > 16 indicates homology
AGATTVNIPDTVGYSTPLEFGR + Deamidated (NQ)
3312 702.7111 2105.1115 2105.1004 5.25 1 5 0.85 +1Score > 37 indicates identity
Score > 16 indicates homology
EGQAILGALPQGADVIALDPR + 2 Deamidated (NQ)
4274 986.5358 2956.5856 2956.5982 -4.26 1 5 0.71 +1Score > 33 indicates identity
Score > 16 indicates homology
NIGIIAHVDHGKTTTSDQLLAASGIISPK
4508 1114.9453 3341.8141 3341.8210 -2.06 1 5 0.45 +1Score > 31 indicates identity
Score > 14 indicates homology
LNHNHMLLWLLLIGTVPVGIAGLLFNKQAE + 2 Deamidated (NQ); Oxidation (M)
4500 834.4540 3333.7869 3333.7793 2.27 0 5 1 +1Score > 32 indicates identity
Score > 17 indicates homology
VQLINQPAHTAPQLIRPAAPAHLSSDSTSSVK
2184 489.6353 1465.8841 1465.8617 15.3 1 5 0.77 +1Score > 30 indicates identity
Score > 16 indicates homology
IIVVVGAPGDKGVSR
3086 487.5046 1945.9893 1945.9646 12.7 1 5 0.93 +1Score > 39 indicates identity
Score > 17 indicates homology
VYAGAAHPHTAQQPQELK + Deamidated (NQ)
3141 995.0335 1988.0524 1988.0367 7.92 1 5 0.71 +1Score > 38 indicates identity
Score > 16 indicates homology
FLEQAQQVSGLLAQLWR + 2 Deamidated (NQ)
2599 826.4330 1650.8514 1650.8577 -3.78 1 5 0.67 +1Score > 40 indicates identity
Score > 15 indicates homology
LSVADVTAAFANTKSR + Deamidated (NQ)
3343 706.7060 2117.0962 2117.0609 16.7 1 5 0.45 +1Score > 39 indicates identity
Score > 14 indicates homology
VNPPDLMNCLTSRTLITR + Deamidated (NQ); Oxidation (M)
2568 545.9698 1634.8876 1634.8628 15.2 1 5 0.85 +1Score > 37 indicates identity
Score > 16 indicates homology
SLHLDPSDILELGAR
3049 641.3571 1921.0495 1921.0706 -11.0 1 5 0.88 +1Score > 36 indicates identity
Score > 16 indicates homology
ILLGSSGSIGKNVAFLAMK + Oxidation (M)
3083 971.5457 1941.0768 1941.0611 8.12 0 5 0.86 +1Score > 35 indicates identity
Score > 16 indicates homology
LIIDWHNLAYSILQLK + 2 Deamidated (NQ)
3561 1119.5864 2237.1582 2237.1467 5.17 1 5 0.82 +1Score > 38 indicates identity
Score > 16 indicates homology
SVQGIQYLPNVTKLFLNGNK + 5 Deamidated (NQ)
3863 825.0994 2472.2764 2472.2642 4.91 1 5 0.42 +1Score > 37 indicates identity
Score > 13 indicates homology
QAVQVASLTTGMASQASETLQVPR
1821 640.8893 1279.7640 1279.7724 -6.55 1 5 0.95 +1Score > 33 indicates identity
Score > 17 indicates homology
RPKIGGLANQVK
3746 788.1195 2361.3367 2361.3743 -16.0 1 5 0.72 +1Score > 30 indicates identity
Score > 16 indicates homology
NIRLSVAAIVTDVLPNGNLIIR + Deamidated (NQ)
2322 765.4394 1528.8642 1528.8865 -14.5 1 5 0.76 +1Score > 36 indicates identity
Score > 16 indicates homology
LAGSLIKPVFLDEK
3709 777.4062 2329.1968 2329.1703 11.4 1 5 1 +1Score > 38 indicates identity
Score > 17 indicates homology
GIQGAAGEPGLFGFLGPKGDLGDR + Deamidated (NQ)
4253 732.3929 2925.5425 2925.5239 6.36 0 5 0.73 +1Score > 34 indicates identity
Score > 16 indicates homology
AMQVAGAVTPVPGGVGQMTVACLLVNTLR + Oxidation (M)
298 576.2962 575.2889 575.2915 -4.43 1 4 0.64 +1Score > 44 indicates identity
Score > 15 indicates homology
AGETAK
3097 653.3529 1957.0369 1957.0594 -11.5 0 4 0.77 +1Score > 38 indicates identity
Score > 16 indicates homology
IGPIANLDAIYMVNVLPK + Deamidated (NQ); Oxidation (M)
3588 563.2863 2249.1161 2249.1189 -1.24 1 4 0.45 +1Score > 39 indicates identity
Score > 14 indicates homology
SATGGFDINNLLGRGSHGSVYK
1524 580.3610 1158.7074 1158.6947 11.0 1 4 0.52 +1Score > 31 indicates identity
Score > 14 indicates homology
MLLSLKWLR
2887 904.5296 1807.0446 1807.0277 9.37 1 4 1 +1Score > 31 indicates identity
Score > 17 indicates homology
QIIPQETINVIMIAPK
2893 906.4737 1810.9328 1810.9676 -19.2 0 4 0.75 +1Score > 39 indicates identity
Score > 16 indicates homology
LGANIAVDGNTALVTGVPK + 2 Deamidated (NQ)
2905 606.9590 1817.8552 1817.8295 14.1 0 4 0.55 +1Score > 40 indicates identity
Score > 14 indicates homology
VFSTNGQSVAFWMSLE + Oxidation (M)
3687 578.8182 2311.2437 2311.2821 -16.6 1 4 0.84 +1Score > 35 indicates identity
Score > 16 indicates homology
TVTLQNVPGLQDVATMLKLIR + 2 Deamidated (NQ)
3243 684.7197 2051.1373 2051.1011 17.6 1 4 0.95 +1Score > 34 indicates identity
Score > 17 indicates homology
VNVPEPGSSTLLQGRDLLR + Deamidated (NQ)
4084 908.4711 2722.3915 2722.3524 14.3 0 4 0.99 +1Score > 37 indicates identity
Score > 17 indicates homology
ADGLFGYQLVVVVDDLDMGVDDIVR
2183 489.6348 1465.8826 1465.8617 14.3 1 4 0.5 +1Score > 30 indicates identity
Score > 14 indicates homology
IIVVVGAPGDKGVSR
2313 509.6393 1525.8961 1525.8716 16.1 1 4 1 +1Score > 32 indicates identity
Score > 17 indicates homology
VVLSQVSLAISPGEK
2482 803.4708 1604.9270 1604.9250 1.29 1 4 1 +1Score > 32 indicates identity
Score > 17 indicates homology
LSPPPGLPSKIGSISR
3678 578.0347 2308.1097 2308.0933 7.09 1 4 0.55 +1Score > 39 indicates identity
Score > 14 indicates homology
FIILSDILGDEDHLGDMDFK + Oxidation (M)
2652 837.4511 1672.8876 1672.9083 -12.3 1 4 0.99 +1Score > 39 indicates identity
Score > 17 indicates homology
ASLEAALCLGFLRPR
2778 581.6686 1741.9840 1741.9549 16.7 1 4 0.95 +1Score > 34 indicates identity
Score > 17 indicates homology
QAIEKPGLFVMKPQR + Deamidated (NQ)
2585 412.2278 1644.8821 1644.8909 -5.36 0 4 0.78 +1Score > 39 indicates identity
Score > 16 indicates homology
TLPDASFLPVLSVMR
2891 906.0003 1809.9860 1809.9949 -4.87 1 4 0.91 +1Score > 37 indicates identity
Score > 16 indicates homology
VPSGPPSTTILASSNKVR
3288 696.3710 2086.0912 2086.1099 -8.97 1 4 0.93 +1Score > 38 indicates identity
Score > 17 indicates homology
LADKPQWLVFNKIDTIGE
3643 765.3975 2293.1707 2293.1372 14.6 1 4 0.44 +1Score > 38 indicates identity
Score > 13 indicates homology
SGKSMTQSLWLNNNVLTDLR + Deamidated (NQ); Oxidation (M)
1708 410.9345 1229.7817 1229.7860 -3.49 0 4 0.85 +1Score > 22 indicates identity
Score > 16 indicates homology
LIPQHLLGIVK
4217 961.1357 2880.3853 2880.3851 0.059 1 4 0.8 +1Score > 37 indicates identity
Score > 16 indicates homology
TAINAPNNYQVAVQIFDGKTSLCEPK + 3 Deamidated (NQ)
3206 1014.5078 2027.0010 2026.9856 7.62 1 4 0.95 +1Score > 39 indicates identity
Score > 17 indicates homology
VNAVNCNTSWKITLFMK + 2 Deamidated (NQ)
3227 680.3466 2038.0180 2038.0380 -9.83 1 4 0.7 +1Score > 39 indicates identity
Score > 15 indicates homology
TPAFMPVGTYGTVKGMLPR + Oxidation (M)
2396 392.4603 1565.8121 1565.8276 -9.88 1 4 0.61 +1Score > 40 indicates identity
Score > 15 indicates homology
MLWFQGKSLQIAK + Deamidated (NQ); Oxidation (M)
3308 700.0080 2097.0022 2097.0300 -13.3 1 4 0.83 +1Score > 39 indicates identity
Score > 16 indicates homology
IATIMQNTDSIFEIDTIR + Deamidated (NQ); Oxidation (M)
4046 673.3504 2689.3725 2689.3303 15.7 1 4 0.8 +1Score > 37 indicates identity
Score > 16 indicates homology
LVDMGEAVGIIAAQSIGEPGTQLTMR + Deamidated (NQ); 2 Oxidation (M)
4234 967.8486 2900.5240 2900.5279 -1.34 1 4 0.49 +1Score > 35 indicates identity
Score > 14 indicates homology
SLQLSFTFLIISIILSQGMMADAQKK + 2 Deamidated (NQ); Oxidation (M)
3460 728.3782 2182.1128 2182.1277 -6.82 1 4 0.85 +1Score > 39 indicates identity
Score > 16 indicates homology
ATRPVQMPGASLASAPTARGSR + Deamidated (NQ)
3467 729.4017 2185.1833 2185.2149 -14.5 1 4 0.61 +1Score > 36 indicates identity
Score > 15 indicates homology
MGPQAAAGRMILLVVLMLSAK + Oxidation (M)
3446 1087.5548 2173.0950 2173.1300 -16.1 1 4 0.44 +1Score > 39 indicates identity
Score > 13 indicates homology
SQGSPSSPSLIQIDMQKIIK + Deamidated (NQ); Oxidation (M)
4544 1144.2623 3429.7651 3429.7101 16.0 0 4 0.54 +1Score > 34 indicates identity
Score > 14 indicates homology
VFGFNNLFLGGCGNIPTAYGANPTLTAMSLAIK + Deamidated (NQ)
2973 933.9299 1865.8452 1865.8353 5.33 1 4 0.82 +1Score > 39 indicates identity
Score > 16 indicates homology
INSVMDETTAGPGKPFGE + Deamidated (NQ); Oxidation (M)
3658 1151.6405 2301.2664 2301.2369 12.9 1 4 0.93 +1Score > 34 indicates identity
Score > 16 indicates homology
SISLDTLIAVLKWSSHPYGSK
3872 827.4279 2479.2619 2479.2125 19.9 1 4 0.67 +1Score > 38 indicates identity
Score > 15 indicates homology
TMARLGISVSDANNLLNNAFGQR + 2 Deamidated (NQ); Oxidation (M)
4122 925.5115 2773.5127 2773.4659 16.9 1 4 0.47 +1Score > 33 indicates identity
Score > 13 indicates homology
RGNVMGIALGGLAMGVLVGPPFGSVLYE
4511 837.4401 3345.7313 3345.7860 -16.4 1 4 0.73 +1Score > 34 indicates identity
Score > 15 indicates homology
GELHSALIGQFNVSNLLLVFATLLSLGYDIE
3522 737.4161 2209.2265 2209.1855 18.5 1 4 0.71 +1Score > 33 indicates identity
Score > 15 indicates homology
RPVPVLTVHPTGNPGEALLGGE
2868 901.5051 1800.9956 1800.9655 16.7 1 4 0.64 +1Score > 36 indicates identity
Score > 15 indicates homology
LVVLGGAMGATTLNAEGVK + Deamidated (NQ)
3518 737.0662 2208.1768 2208.1526 11.0 1 4 0.68 +1Score > 37 indicates identity
Score > 15 indicates homology
YSLQNDIQTLVTVTTVGVEK + Deamidated (NQ)
3095 653.0315 1956.0727 1956.0568 8.12 0 4 1 +1Score > 36 indicates identity
Score > 17 indicates homology
LSDLISSAIVDDPPFVLR
4053 898.4485 2692.3237 2692.2724 19.1 1 4 0.84 +1Score > 38 indicates identity
Score > 16 indicates homology
MANSFAARIFTTLSDLQTNMANLK + 3 Deamidated (NQ); 2 Oxidation (M)
2638 557.3273 1668.9601 1668.9271 19.7 1 4 0.81 +1Score > 34 indicates identity
Score > 16 indicates homology
ADGQLVTGGSILVRQR
2964 620.6562 1858.9468 1858.9173 15.8 1 4 0.49 +1Score > 40 indicates identity
Score > 14 indicates homology
INRDGDVSDIAVPFANR + Deamidated (NQ)
3819 808.4584 2422.3534 2422.3835 -12.4 1 4 1 +1Score > 31 indicates identity
Score > 17 indicates homology
TKPVFVVATANNVDLLPLEIIR + Deamidated (NQ)
4301 750.6528 2998.5821 2998.5361 15.3 1 4 0.85 +1Score > 34 indicates identity
Score > 16 indicates homology
LSANILFDVTLAQEIYAASDIFLMPSR + Deamidated (NQ)
4717 901.1114 4500.5206 4500.4788 9.30 1 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
VPYIIIGQLATIFYFSYFMILLPLISILENMLFYLAIK + Deamidated (NQ); Oxidation (M)
1146 497.2736 992.5326 992.5403 -7.75 1 4 0.7 +1Score > 40 indicates identity
Score > 15 indicates homology
VATGFGRTGK
2135 717.3950 1432.7754 1432.7496 18.0 1 4 0.8 +1Score > 39 indicates identity
Score > 16 indicates homology
LQYHMQLRSIK + Deamidated (NQ); Oxidation (M)
3611 756.3945 2266.1617 2266.1706 -3.92 1 4 0.86 +1Score > 38 indicates identity
Score > 16 indicates homology
IWDTINGPNLAQNVLPTRSR + 2 Deamidated (NQ)
3875 827.4715 2479.3927 2479.3760 6.73 0 4 1 +1Score > 30 indicates identity
Score > 17 indicates homology
IYDSQIYTVLCIALLAGILAIR + Deamidated (NQ)
3970 861.1380 2580.3922 2580.3622 11.6 1 4 1 +1Score > 34 indicates identity
Score > 17 indicates homology
YIGLNPVIVHGGGPQIGQVLKSMGK + 3 Deamidated (NQ); Oxidation (M)
4033 892.7857 2675.3353 2675.2870 18.1 0 4 0.91 +1Score > 38 indicates identity
Score > 16 indicates homology
VNGHIIDDIMHDIMVKPLPQGCR + 2 Deamidated (NQ); Oxidation (M)
2645 557.9247 1670.7523 1670.7635 -6.72 1 4 0.96 +1Score > 39 indicates identity
Score > 17 indicates homology
EAQLDIQSQSQPPTE + Deamidated (NQ)
3073 645.6628 1933.9666 1933.9606 3.09 1 4 0.8 +1Score > 40 indicates identity
Score > 16 indicates homology
ALRNATQGNSTLFGNDVR + Deamidated (NQ)
4115 922.1371 2763.3895 2763.4331 -15.8 1 4 0.63 +1Score > 38 indicates identity
Score > 15 indicates homology
DQAFVSSLVPNYGGTLDIIGGVVVESK
4131 696.0989 2780.3665 2780.3257 14.7 1 4 0.45 +1Score > 38 indicates identity
Score > 13 indicates homology
YGQEPQVAMMMPILPGLDGVQKMSK + Deamidated (NQ); 2 Oxidation (M)
4314 1007.7922 3020.3548 3020.3227 10.6 1 4 0.49 +1Score > 35 indicates identity
Score > 14 indicates homology
GLPGDRMSSMTINFSAQTSSNSLQDTIE + 2 Deamidated (NQ); 2 Oxidation (M)
3128 661.6809 1982.0209 1982.0010 10.0 0 4 0.92 +1Score > 39 indicates identity
Score > 16 indicates homology
VNFAVQAFAALNSNNFVR + Deamidated (NQ)
3606 1134.0797 2266.1448 2266.1780 -14.6 1 4 0.58 +1Score > 39 indicates identity
Score > 14 indicates homology
VQLTQQFCKLFQALGLNTR + 2 Deamidated (NQ)
2220 494.9475 1481.8207 1481.8242 -2.37 1 4 0.79 +1Score > 38 indicates identity
Score > 16 indicates homology
KITLFSIYSPASR
2320 765.4337 1528.8528 1528.8725 -12.9 1 4 0.93 +1Score > 37 indicates identity
Score > 16 indicates homology
QIRNPLAGIVFSSK
2643 557.3378 1668.9916 1668.9847 4.09 1 4 0.64 +1Score > 30 indicates identity
Score > 15 indicates homology
ALSLAKPIIQMLKNK + 2 Deamidated (NQ)
4304 752.3759 3005.4745 3005.4539 6.84 0 4 1 +1Score > 37 indicates identity
Score > 17 indicates homology
WSQTLPTTNVPSIIVDQSMLSQTNSIE + Deamidated (NQ); Oxidation (M)
2786 586.6042 1756.7908 1756.7726 10.3 0 4 0.61 +1Score > 39 indicates identity
Score > 14 indicates homology
NGGSMADPGSVAYLFNR + 2 Deamidated (NQ)
1823 640.8899 1279.7652 1279.7724 -5.61 1 4 0.9 +1Score > 33 indicates identity
Score > 16 indicates homology
RPKIGGLANQVK
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