MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2796 588.3156 1761.9250 1761.9050 11.3 1 5 1 +1Score > 39 indicates identity
Score > 18 indicates homology
ITATNVSFVHKGVFGGE
3031 477.7145 1906.8289 1906.8513 -11.7 1 5 0.87 +1Score > 36 indicates identity
Score > 17 indicates homology
IENAIMQGLQQCSSGGGR + 2 Deamidated (NQ)
1472 569.3588 1136.7030 1136.6805 19.9 0 5 0.41 +1Score > 30 indicates identity
Score > 14 indicates homology
QIIAPALVALE
2682 566.2923 1695.8551 1695.8315 13.9 1 5 0.7 +1Score > 40 indicates identity
Score > 16 indicates homology
NTGILTVTYNSEVQR + 2 Deamidated (NQ)
2942 615.9901 1844.9485 1844.9302 9.90 1 5 0.84 +1Score > 40 indicates identity
Score > 17 indicates homology
QGQAIMVDVSGKEPTLR + Deamidated (NQ); Oxidation (M)
2768 578.6682 1732.9828 1732.9764 3.70 0 5 1 +1Score > 33 indicates identity
Score > 18 indicates homology
FDTNVPVIFLIGSLAK
3025 952.4968 1902.9790 1902.9687 5.44 1 5 0.87 +1Score > 39 indicates identity
Score > 17 indicates homology
VLQQNLPTEDIGYSGLR + Deamidated (NQ)
3902 837.4684 2509.3834 2509.4115 -11.2 1 5 0.61 +1Score > 32 indicates identity
Score > 16 indicates homology
SLSLLANTTQTHIRTIDGIILTK + Deamidated (NQ)
1610 596.8084 1191.6022 1191.6023 -0.039 0 5 0.83 +1Score > 42 indicates identity
Score > 17 indicates homology
GIYILSPSNVE + Deamidated (NQ)
2748 864.4963 1726.9780 1726.9577 11.8 1 5 0.76 +1Score > 35 indicates identity
Score > 17 indicates homology
ALLVQGDNGAGKTTLLR + Deamidated (NQ)
2772 869.9744 1737.9342 1737.9050 16.9 1 5 0.88 +1Score > 38 indicates identity
Score > 17 indicates homology
VIASYNQLSSFRQPK + Deamidated (NQ)
2166 728.4348 1454.8550 1454.8457 6.45 1 5 0.96 +1Score > 33 indicates identity
Score > 18 indicates homology
TAIRALQAVGLDVK + Deamidated (NQ)
1131 983.5111 982.5038 982.5124 -8.70 0 5 0.88 +1Score > 39 indicates identity
Score > 17 indicates homology
DVAQFLYK
3179 671.3325 2010.9757 2010.9608 7.39 1 5 0.87 +1Score > 40 indicates identity
Score > 17 indicates homology
AYEADPTSIFGGIIALNME
3865 826.0764 2475.2074 2475.2364 -11.7 1 5 0.86 +1Score > 38 indicates identity
Score > 17 indicates homology
NGSCYRMLGYLALVMIPPLYK + Deamidated (NQ); Oxidation (M)
4606 927.4732 3705.8637 3705.8638 -0.024 1 5 1 +1Score > 34 indicates identity
Score > 18 indicates homology
SIGLAQAAHSPLAPSYQASPHINAQDSLLQRSVFK + 4 Deamidated (NQ)
2533 809.3950 1616.7754 1616.7504 15.5 0 5 0.89 +1Score > 40 indicates identity
Score > 17 indicates homology
QMVQSYQFGANSIK + Deamidated (NQ); Oxidation (M)
2660 837.4791 1672.9436 1672.9624 -11.2 1 5 0.9 +1Score > 35 indicates identity
Score > 17 indicates homology
RIGIFDSGIGGLTVLR
3126 661.3121 1980.9145 1980.8999 7.33 1 5 0.75 +1Score > 39 indicates identity
Score > 17 indicates homology
GDFLNYALSLMRSHNDE
4562 886.9464 3543.7565 3543.7458 3.00 1 5 0.37 +1Score > 35 indicates identity
Score > 13 indicates homology
TTALFTAGFTLLTAFLFHSNFAEGVNSLMFMK + Deamidated (NQ); Oxidation (M)
4573 894.4978 3573.9621 3573.9480 3.95 1 5 0.84 +1Score > 29 indicates identity
Score > 17 indicates homology
MIALGADTILLGRAFLYALATAGQAGVANLLNLIE + Deamidated (NQ); Oxidation (M)
2255 750.4054 1498.7962 1498.7919 2.90 0 5 0.85 +1Score > 40 indicates identity
Score > 17 indicates homology
TGLINPVAIFEPVE + Deamidated (NQ)
2728 860.4283 1718.8420 1718.8331 5.20 1 5 0.65 +1Score > 41 indicates identity
Score > 16 indicates homology
AVNVNMGKGNMAATIVE + Deamidated (NQ)
3045 639.9859 1916.9359 1916.9335 1.22 1 5 0.84 +1Score > 40 indicates identity
Score > 17 indicates homology
KNPNLVLGIIGCMAQNR + 4 Deamidated (NQ); Oxidation (M)
3852 822.1154 2463.3244 2463.3009 9.53 0 5 0.62 +1Score > 35 indicates identity
Score > 16 indicates homology
IAVAHYAAQLSTLDINPQPLSLE
2677 565.2858 1692.8356 1692.8141 12.7 1 5 0.94 +1Score > 41 indicates identity
Score > 17 indicates homology
DPTFISKQVLNDCR + Deamidated (NQ)
3301 1047.5422 2093.0698 2093.0476 10.6 1 5 0.9 +1Score > 39 indicates identity
Score > 17 indicates homology
RPTPEMLLHHTFLSGADR + Oxidation (M)
3934 849.7755 2546.3047 2546.2751 11.6 1 5 0.96 +1Score > 37 indicates identity
Score > 17 indicates homology
GPSPLDPLLPSVAQAVQQLQAQER + 5 Deamidated (NQ)
2840 597.3010 1788.8812 1788.8907 -5.34 0 5 0.95 +1Score > 40 indicates identity
Score > 17 indicates homology
GPSANPSAHPVPTYAPAR
2914 608.9926 1823.9560 1823.9417 7.80 1 5 0.66 +1Score > 38 indicates identity
Score > 16 indicates homology
TAQKLAFQAAVSDAFQK + Deamidated (NQ)
1883 657.2759 1312.5372 1312.5289 6.37 0 5 0.76 +1Score > 37 indicates identity
Score > 16 indicates homology
DGSGCGACFQVR
3705 775.4303 2323.2691 2323.2940 -10.7 1 5 0.59 +1Score > 34 indicates identity
Score > 15 indicates homology
WLPLTGKTLDVGTGAGFPGVPLK
1303 530.7457 1059.4768 1059.4832 -6.02 0 5 0.72 +1Score > 39 indicates identity
Score > 16 indicates homology
AAVNQLQNSN + 2 Deamidated (NQ)
4486 662.3703 3306.8151 3306.8380 -6.91 1 5 0.85 +1Score > 30 indicates identity
Score > 17 indicates homology
NFLLLLLAAYALGSTPFAIVSSRLFGLADPR + Deamidated (NQ)
4529 849.9301 3395.6913 3395.6953 -1.17 1 5 0.98 +1Score > 35 indicates identity
Score > 18 indicates homology
ACGTNVTDIVVLVVAADDGIMPQTIESINHVK + Deamidated (NQ); Oxidation (M)
1019 940.5822 939.5749 939.5865 -12.4 1 5 0.87 +1Score > 35 indicates identity
Score > 17 indicates homology
GLTKGLVPR
2436 791.3908 1580.7670 1580.7651 1.26 0 5 0.54 +1Score > 41 indicates identity
Score > 15 indicates homology
MSNATQLVAQTVMR + 2 Oxidation (M)
2382 520.6258 1558.8556 1558.8249 19.7 1 5 0.83 +1Score > 38 indicates identity
Score > 17 indicates homology
QNQALNAMLIKGSR + Oxidation (M)
3956 856.4610 2566.3612 2566.3618 -0.23 1 5 0.47 +1Score > 36 indicates identity
Score > 14 indicates homology
IAQMLTAPFVYYAVGQGALAVEVR
1264 519.7725 1037.5304 1037.5366 -5.96 1 5 0.93 +1Score > 40 indicates identity
Score > 17 indicates homology
RIDDHVQR
3624 1139.5983 2277.1820 2277.1398 18.6 1 5 0.79 +1Score > 38 indicates identity
Score > 17 indicates homology
DAILMNMPNAVISLHRYFR + Deamidated (NQ); Oxidation (M)
2690 567.3217 1698.9433 1698.9668 -13.9 0 5 0.36 +1Score > 36 indicates identity
Score > 13 indicates homology
LLKPSLALNLSDPYR
2925 611.3383 1830.9931 1830.9648 15.4 1 5 0.74 +1Score > 37 indicates identity
Score > 16 indicates homology
IMEPLVLAGDKITSSIE + Oxidation (M)
4102 917.4767 2749.4083 2749.3666 15.2 1 5 0.91 +1Score > 37 indicates identity
Score > 17 indicates homology
LSTQKAALNLGMNVMVLDINQGAWK + 3 Deamidated (NQ); 2 Oxidation (M)
3429 1083.5778 2165.1410 2165.1813 -18.6 1 5 0.78 +1Score > 37 indicates identity
Score > 16 indicates homology
ANPTAAILSACLMLRHLGLK + Oxidation (M)
3692 579.5699 2314.2505 2314.2757 -10.9 1 5 1 +1Score > 35 indicates identity
Score > 18 indicates homology
VLHLGQDDLPLTVARQIVGDR
3745 591.3262 2361.2757 2361.2580 7.48 1 5 0.58 +1Score > 35 indicates identity
Score > 15 indicates homology
GFPPKLISITVGDTDAVDVYVR
3679 770.3973 2308.1701 2308.1546 6.68 1 5 0.69 +1Score > 38 indicates identity
Score > 16 indicates homology
QGVLLLNTSLTVEDGQPASHAR + 3 Deamidated (NQ)
3136 663.3504 1987.0294 1986.9898 19.9 1 5 0.89 +1Score > 39 indicates identity
Score > 17 indicates homology
NIEADVINDLVDQYIPR + Deamidated (NQ)
3700 774.0625 2319.1657 2319.1760 -4.45 0 5 0.96 +1Score > 39 indicates identity
Score > 17 indicates homology
FVLQIAANYGGQWDIAQAAQR
3023 634.3521 1900.0345 1900.0517 -9.07 1 5 0.85 +1Score > 37 indicates identity
Score > 17 indicates homology
SKIIGIDLGTTNSAVAVLE
3671 769.7060 2306.0962 2306.1147 -8.05 1 5 0.51 +1Score > 39 indicates identity
Score > 15 indicates homology
HQPDIVICVGQAGGRMQITPE + Deamidated (NQ)
3151 666.3336 1995.9790 1995.9843 -2.67 1 5 0.81 +1Score > 40 indicates identity
Score > 17 indicates homology
FLLHYNFPPYSVGETGR
3379 713.7105 2138.1097 2138.1398 -14.1 1 5 0.8 +1Score > 38 indicates identity
Score > 17 indicates homology
IANLVYTYILLEGLLDVSE + Deamidated (NQ)
4267 737.8779 2947.4825 2947.4420 13.8 1 5 0.52 +1Score > 37 indicates identity
Score > 15 indicates homology
RADGYHQVVMVMQTIALADTVTVAVNE + Deamidated (NQ); Oxidation (M)
2742 432.2235 1724.8649 1724.8468 10.5 1 5 0.88 +1Score > 40 indicates identity
Score > 17 indicates homology
DLETQVLAQLSSYTR + 2 Deamidated (NQ)
3344 530.2828 2117.1021 2117.0752 12.7 0 5 0.78 +1Score > 38 indicates identity
Score > 16 indicates homology
ALTNAIGNLNIQDAYADALR + Deamidated (NQ)
3681 578.2996 2309.1693 2309.1249 19.2 1 5 0.99 +1Score > 38 indicates identity
Score > 17 indicates homology
AANALDFETMPIQADLSLFDK
2603 828.4756 1654.9366 1654.9042 19.6 1 5 0.8 +1Score > 35 indicates identity
Score > 16 indicates homology
AWDNNVILKLDLNK
565 723.3608 722.3535 722.3633 -13.5 0 5 0.59 +1Score > 41 indicates identity
Score > 15 indicates homology
TTLMNK + Oxidation (M)
4372 780.9206 3119.6533 3119.6372 5.16 1 5 0.71 +1Score > 34 indicates identity
Score > 16 indicates homology
APGLPTAMDRPHFIGIGGAGMSGIAKILAQR + Deamidated (NQ); Oxidation (M)
2329 512.2603 1533.7591 1533.7353 15.5 1 5 0.86 +1Score > 41 indicates identity
Score > 17 indicates homology
RMMTAAAMTYVIK + 3 Oxidation (M)
362 618.3073 617.3000 617.3020 -3.28 0 5 0.87 +1Score > 45 indicates identity
Score > 17 indicates homology
AGTGIAE
4282 990.1880 2967.5422 2967.5077 11.6 1 5 0.79 +1Score > 35 indicates identity
Score > 16 indicates homology
TVVLSDTTINISWSEPLEPNGPLESIR + Deamidated (NQ)
1897 440.2636 1317.7690 1317.7768 -5.96 1 5 0.77 +1Score > 33 indicates identity
Score > 16 indicates homology
LAAIKSLVNYAR
2308 760.4190 1518.8234 1518.8116 7.82 0 5 0.91 +1Score > 38 indicates identity
Score > 17 indicates homology
LIDSIINAMSWLK + Oxidation (M)
2484 402.4813 1605.8961 1605.8695 16.6 1 5 0.92 +1Score > 35 indicates identity
Score > 17 indicates homology
VMTRTTTALVMQVR
2577 547.2958 1638.8656 1638.8941 -17.4 1 5 0.54 +1Score > 39 indicates identity
Score > 15 indicates homology
VRDLKPNGANILVTE + Deamidated (NQ)
3417 718.7194 2153.1364 2153.1633 -12.5 0 5 0.82 +1Score > 38 indicates identity
Score > 17 indicates homology
RPQIQTIAPGAQIAHFLYK + 2 Deamidated (NQ)
4346 1023.5303 3067.5691 3067.5158 17.4 1 5 0.52 +1Score > 35 indicates identity
Score > 15 indicates homology
MNYISTTTITTTNNIDLNNLLPIENIK + 4 Deamidated (NQ)
3020 632.3570 1894.0492 1894.0173 16.8 1 5 0.4 +1Score > 35 indicates identity
Score > 13 indicates homology
RNVPGHVLQRPSYLTR + 2 Deamidated (NQ)
4434 1070.5562 3208.6468 3208.6438 0.94 1 5 0.69 +1Score > 35 indicates identity
Score > 16 indicates homology
QQQALAASLPDVAVELAMSYGQPALPQAIAR + 2 Deamidated (NQ)
3812 605.3224 2417.2605 2417.2187 17.3 0 5 0.74 +1Score > 37 indicates identity
Score > 16 indicates homology
QLLDQAGLQPGDVAAIGAHGQTVR + 3 Deamidated (NQ)
4397 792.4233 3165.6641 3165.6169 14.9 1 5 1 +1Score > 34 indicates identity
Score > 17 indicates homology
KLNIQPDVIIGCHGGGSNFGGTILPFIPDK + 2 Deamidated (NQ)
4232 967.5089 2899.5049 2899.4967 2.82 1 5 0.62 +1Score > 35 indicates identity
Score > 15 indicates homology
LELNDVVYLIRPDPLSYLPNSEPSR + Deamidated (NQ)
4327 760.3899 3037.5305 3037.5425 -3.96 0 5 1 +1Score > 36 indicates identity
Score > 17 indicates homology
AAIGLALLVMMSNTYGLDLIQNLSLLQC + 3 Deamidated (NQ)
4155 702.8943 2807.5481 2807.5605 -4.43 1 5 0.53 +1Score > 31 indicates identity
Score > 15 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
4219 961.4694 2881.3864 2881.3838 0.91 1 5 0.39 +1Score > 37 indicates identity
Score > 13 indicates homology
ISHDAGTMASLLPAVSNTTTENVMYVK + 2 Oxidation (M)
2683 566.2967 1695.8683 1695.8726 -2.56 1 5 0.85 +1Score > 40 indicates identity
Score > 17 indicates homology
TLGFNGLARMSSSSLR
3177 671.0389 2010.0949 2010.0898 2.51 1 5 1 +1Score > 36 indicates identity
Score > 17 indicates homology
LASIKGLHDIGTFVAPDTR
3298 1046.5621 2091.1096 2091.1034 2.99 1 5 0.54 +1Score > 37 indicates identity
Score > 15 indicates homology
VLADPMYNSIVVTRLINR + 2 Deamidated (NQ); Oxidation (M)
3382 714.3864 2140.1374 2140.1164 9.80 0 5 0.89 +1Score > 37 indicates identity
Score > 17 indicates homology
GLDVPQVSLIINYDLPNNR + Deamidated (NQ)
3707 776.4152 2326.2238 2326.1804 18.6 1 5 0.5 +1Score > 37 indicates identity
Score > 14 indicates homology
LGNNLPTPQEVVALYNQANIR + 3 Deamidated (NQ)
2562 408.7470 1630.9589 1630.9406 11.2 0 5 0.82 +1Score > 31 indicates identity
Score > 16 indicates homology
LIQLGVFTGLSNLTR
3437 724.7311 2171.1715 2171.1741 -1.19 0 5 0.79 +1Score > 36 indicates identity
Score > 16 indicates homology
YLPLVATLGLFIFFANLME
3457 546.2883 2181.1241 2181.1286 -2.05 1 5 0.4 +1Score > 38 indicates identity
Score > 13 indicates homology
VAEMVAMAGALVLNIGTLDHR + Deamidated (NQ)
4290 747.8804 2987.4925 2987.4975 -1.67 1 5 0.53 +1Score > 37 indicates identity
Score > 15 indicates homology
GGLLVVDASQGVEAQTLANVYLAIDNNLE + 2 Deamidated (NQ)
2162 726.3790 1450.7434 1450.7303 9.03 1 5 0.64 +1Score > 41 indicates identity
Score > 15 indicates homology
DIDAILDQYRTK + Deamidated (NQ)
2501 805.9794 1609.9442 1609.9443 -0.035 0 5 1 +1Score > 32 indicates identity
Score > 17 indicates homology
YIGPLGILAPLADGLK
3246 685.3853 2053.1341 2053.1176 8.01 1 5 0.92 +1Score > 34 indicates identity
Score > 17 indicates homology
GGIVGMTLPIARDLAPMGIR + Oxidation (M)
3401 717.0195 2148.0367 2148.0519 -7.11 1 5 0.61 +1Score > 39 indicates identity
Score > 15 indicates homology
GSVQGSSRAASVSVQTTQQNR + Deamidated (NQ)
3748 789.4012 2365.1818 2365.1584 9.89 1 5 0.94 +1Score > 38 indicates identity
Score > 17 indicates homology
DGLPMTLPVRLGADADSANPGGPK + Deamidated (NQ); Oxidation (M)
1985 456.2301 1365.6685 1365.6598 6.32 1 5 0.92 +1Score > 41 indicates identity
Score > 17 indicates homology
WDLIASGSMDKK + Oxidation (M)
4075 678.6071 2710.3993 2710.3564 15.8 0 5 0.61 +1Score > 36 indicates identity
Score > 15 indicates homology
SANLMLSLTVLPNVNSTWYFDVPK + 2 Deamidated (NQ)
3615 758.3867 2272.1383 2272.1133 11.0 1 5 0.59 +1Score > 39 indicates identity
Score > 15 indicates homology
SLKPWDAVVSKFTGCFQMR + Oxidation (M)
3366 712.0538 2133.1396 2133.1365 1.44 0 5 0.64 +1Score > 37 indicates identity
Score > 15 indicates homology
TVGAKPVVVHGGGPQISDMLR + Oxidation (M)
3883 830.4703 2488.3891 2488.3723 6.75 1 5 0.74 +1Score > 31 indicates identity
Score > 16 indicates homology
ALQQLKAMGYLLVLVTNQSGIAR + 2 Deamidated (NQ)
1723 618.3071 1234.5996 1234.5976 1.68 1 5 0.73 +1Score > 42 indicates identity
Score > 16 indicates homology
GMNVEQATTLR + Oxidation (M)
2838 596.3294 1785.9664 1785.9659 0.29 1 5 0.86 +1Score > 38 indicates identity
Score > 17 indicates homology
ADILVAAVGRLQMITAE + Oxidation (M)
3640 765.3931 2293.1575 2293.1131 19.3 0 5 0.91 +1Score > 38 indicates identity
Score > 17 indicates homology
FGLALMMLMMAVAFFNDVTR + Oxidation (M)
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