MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3683 1155.6549 2309.2952 2309.2718 10.2 1 6 0.7 +1Score > 32 indicates identity
Score > 17 indicates homology
QKHPNIPIGLLMYANLVFNK
2414 787.9196 1573.8246 1573.8351 -6.66 1 6 0.69 +1Score > 40 indicates identity
Score > 17 indicates homology
IIQQYIENPLSQK + Deamidated (NQ)
2750 864.9781 1727.9416 1727.9094 18.7 0 6 0.83 +1Score > 38 indicates identity
Score > 18 indicates homology
LFNGGPVGLNTLAAALAE + Deamidated (NQ)
3759 595.7888 2379.1261 2379.0988 11.5 1 6 0.94 +1Score > 39 indicates identity
Score > 18 indicates homology
VAVVGAMSGPIAQWGDMEFNGAR + Deamidated (NQ); Oxidation (M)
3925 845.4872 2533.4398 2533.4681 -11.2 1 6 0.39 +1Score > 29 indicates identity
Score > 14 indicates homology
WPPFLLGCTILVILLVMKHVGK
3914 631.8165 2523.2369 2523.2677 -12.2 1 6 0.72 +1Score > 38 indicates identity
Score > 17 indicates homology
QSRLGPQPTQGQLAGLQQGGSGSIR + 3 Deamidated (NQ)
2302 759.9166 1517.8186 1517.8309 -8.09 0 6 0.66 +1Score > 39 indicates identity
Score > 17 indicates homology
LLKPTVMMNLLGR + Deamidated (NQ); 2 Oxidation (M)
3730 1175.5830 2349.1514 2349.1488 1.11 1 6 0.88 +1Score > 39 indicates identity
Score > 18 indicates homology
FGITSQGELILADVIDNDSWR + Deamidated (NQ)
890 443.7389 885.4632 885.4556 8.66 1 6 0.65 +1Score > 41 indicates identity
Score > 16 indicates homology
TASASGPKAP
2621 833.9927 1665.9708 1665.9777 -4.13 1 6 0.77 +1Score > 32 indicates identity
Score > 17 indicates homology
LGIPLLKALQTQASGR + Deamidated (NQ)
2804 883.4763 1764.9380 1764.9444 -3.58 0 6 0.64 +1Score > 38 indicates identity
Score > 16 indicates homology
AIVNLQMLTYLNLSR + Deamidated (NQ); Oxidation (M)
3078 647.0386 1938.0940 1938.0608 17.1 0 6 0.85 +1Score > 33 indicates identity
Score > 18 indicates homology
DAIIVGCAQVLALVPGVSR + Deamidated (NQ)
3229 680.3715 2038.0927 2038.0833 4.58 1 6 0.32 +1Score > 37 indicates identity
Score > 13 indicates homology
VNIATLPLQVNINVQRLE + 5 Deamidated (NQ)
3614 1137.0764 2272.1382 2272.1786 -17.8 1 6 0.79 +1Score > 39 indicates identity
Score > 17 indicates homology
DNVYRNVMPTLVIPAHYVR + Oxidation (M)
4254 586.3088 2926.5076 2926.4560 17.7 1 6 1 +1Score > 36 indicates identity
Score > 18 indicates homology
EPETYGHLTLNDIVQSLSADAQALNVK + Deamidated (NQ)
1494 1145.6095 1144.6022 1144.5910 9.79 1 6 0.53 +1Score > 41 indicates identity
Score > 16 indicates homology
AKAQAMGIPDK + Oxidation (M)
1955 450.2760 1347.8062 1347.8126 -4.75 1 6 0.69 +1Score > 30 indicates identity
Score > 17 indicates homology
GEILPGIPNVLVK
2864 901.4843 1800.9540 1800.9372 9.35 0 6 0.87 +1Score > 39 indicates identity
Score > 18 indicates homology
MGILTVAIVTTPFYFE
3212 677.3682 2029.0828 2029.0489 16.7 0 6 0.67 +1Score > 37 indicates identity
Score > 17 indicates homology
HFGMIAGGTGITPMLQIIR + Deamidated (NQ); Oxidation (M)
3877 828.0818 2481.2236 2481.2421 -7.46 1 6 0.88 +1Score > 38 indicates identity
Score > 18 indicates homology
SNDRLLQNVVSILLMTGYNVSE + Deamidated (NQ); Oxidation (M)
4055 898.4520 2692.3342 2692.3391 -1.83 1 6 0.48 +1Score > 38 indicates identity
Score > 15 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
891 887.4591 886.4518 886.4648 -14.6 1 6 0.8 +1Score > 42 indicates identity
Score > 17 indicates homology
IPTELVSE
3041 638.9927 1913.9563 1913.9192 19.3 1 6 0.71 +1Score > 40 indicates identity
Score > 17 indicates homology
ANFVYNTSNMKPKQLR + 4 Deamidated (NQ)
482 684.3226 683.3153 683.3061 13.5 0 6 0.52 +1Score > 37 indicates identity
Score > 15 indicates homology
TGFGMR + Oxidation (M)
4230 966.1472 2895.4198 2895.4477 -9.63 1 6 1 +1Score > 37 indicates identity
Score > 18 indicates homology
FTDLFNLINPGDLMVFNNTRVIPAR + 3 Deamidated (NQ); Oxidation (M)
3493 733.0219 2196.0439 2196.0442 -0.15 1 6 0.54 +1Score > 39 indicates identity
Score > 16 indicates homology
TATIDKNNMMQYIAPQDLK + 2 Deamidated (NQ)
4134 929.5165 2785.5277 2785.5059 7.83 0 6 1 +1Score > 32 indicates identity
Score > 18 indicates homology
MLPVLFVTFVPSMMLAQIMTLFIK + Oxidation (M)
2102 708.4041 1414.7936 1414.7780 11.1 0 6 0.96 +1Score > 38 indicates identity
Score > 18 indicates homology
SVILLTLGTNDNR
2076 703.8950 1405.7754 1405.7678 5.47 1 6 0.93 +1Score > 37 indicates identity
Score > 18 indicates homology
VKDVSQEPLVHR
3459 728.0613 2181.1621 2181.1641 -0.92 1 6 0.77 +1Score > 37 indicates identity
Score > 17 indicates homology
ATTPLTAATSNPSTPAKPAKQK + Deamidated (NQ)
3894 835.0892 2502.2458 2502.2649 -7.64 1 6 0.56 +1Score > 38 indicates identity
Score > 16 indicates homology
LLTNDIWGDMINLGAGSASNRIR + Oxidation (M)
4326 1013.5167 3037.5283 3037.5066 7.13 0 6 1 +1Score > 36 indicates identity
Score > 18 indicates homology
GTAIYDLPATNGVPIVADMSSNILAAQYR + Deamidated (NQ); Oxidation (M)
1367 546.8096 1091.6046 1091.5863 16.8 1 6 0.58 +1Score > 39 indicates identity
Score > 16 indicates homology
IFGEDGLTLK
3046 640.0101 1917.0085 1917.0030 2.88 1 6 0.57 +1Score > 38 indicates identity
Score > 16 indicates homology
QIPELPDPNLDMGILPR
3165 1004.5898 2007.1650 2007.1405 12.3 1 6 0.61 +1Score > 29 indicates identity
Score > 16 indicates homology
IVLGIDPALGSLGWAVVAKE
2648 558.2847 1671.8323 1671.8138 11.1 1 6 0.66 +1Score > 40 indicates identity
Score > 16 indicates homology
QTEYGSLTALVSTMR + Oxidation (M)
3895 835.4490 2503.3252 2503.3331 -3.18 1 6 0.72 +1Score > 36 indicates identity
Score > 17 indicates homology
GMGGTLGFLAVVVALGAMFGKILHE + Oxidation (M)
2018 461.9233 1382.7481 1382.7405 5.45 1 6 0.98 +1Score > 39 indicates identity
Score > 18 indicates homology
SSELTPTPPLVSR
2020 692.8259 1383.6372 1383.6374 -0.13 0 6 0.61 +1Score > 40 indicates identity
Score > 16 indicates homology
VVSSGVTGAMDMAK + 2 Oxidation (M)
3631 761.4043 2281.1911 2281.1988 -3.38 0 6 0.51 +1Score > 37 indicates identity
Score > 15 indicates homology
AMQVALINDGPVTLILDTPQR + Deamidated (NQ); Oxidation (M)
3105 492.2864 1965.1165 1965.0843 16.4 1 6 0.39 +1Score > 32 indicates identity
Score > 14 indicates homology
HAVLRGHLHIADMVQIR
3283 1043.5345 2085.0544 2085.0524 0.97 0 6 0.89 +1Score > 39 indicates identity
Score > 18 indicates homology
ATIVANAGQPGAVTIATNMAGR + 2 Deamidated (NQ)
4373 1042.1721 3123.4945 3123.5320 -12.0 1 6 0.8 +1Score > 36 indicates identity
Score > 17 indicates homology
TNSTTTIINNNTQNNFTNITNIIVSKNE + Deamidated (NQ)
2070 468.9231 1403.7475 1403.7310 11.8 0 6 1 +1Score > 40 indicates identity
Score > 18 indicates homology
QWVPPAPRPAQR + 2 Deamidated (NQ)
3703 775.3992 2323.1758 2323.2068 -13.4 1 6 0.59 +1Score > 38 indicates identity
Score > 16 indicates homology
MLLYYLHRAQQPITLTAMK + Deamidated (NQ); 2 Oxidation (M)
4760 819.4399 5729.0284 5729.0017 4.66 1 6 1 +1Score > 26 indicates identity
Score > 18 indicates homology
LFGSLGFGVAVFAMGQVTNQLYPIHVIFIFGCAFLCIAAILASQVPGQQKTTK + Deamidated (NQ)
3108 984.0107 1966.0068 1966.0081 -0.63 1 6 1 +1Score > 39 indicates identity
Score > 18 indicates homology
NLGAIQVLPKMTSHIVNE + 3 Deamidated (NQ)
3630 761.4024 2281.1854 2281.2165 -13.7 1 6 0.81 +1Score > 38 indicates identity
Score > 17 indicates homology
VGEAVGIIAAQSIGEPGTQLTLR + 2 Deamidated (NQ)
543 708.3698 707.3625 707.3602 3.27 0 6 0.65 +1Score > 43 indicates identity
Score > 16 indicates homology
DAYAIR
1582 591.3683 1180.7220 1180.7179 3.48 1 6 0.89 +1Score > 31 indicates identity
Score > 18 indicates homology
KLLQVDVPLR + Deamidated (NQ)
2642 557.3373 1668.9901 1668.9847 3.19 1 6 0.84 +1Score > 30 indicates identity
Score > 17 indicates homology
ALSLAKPIIQMLKNK + 2 Deamidated (NQ)
2877 902.4736 1802.9326 1802.9124 11.2 1 6 0.97 +1Score > 40 indicates identity
Score > 18 indicates homology
KFMTLDPVSQDALPPK + Deamidated (NQ); Oxidation (M)
2943 616.3126 1845.9160 1845.9254 -5.13 0 6 0.62 +1Score > 40 indicates identity
Score > 16 indicates homology
AVVIACNTATSAAVNDLR + Deamidated (NQ)
4439 1071.2043 3210.5911 3210.6019 -3.38 1 6 1 +1Score > 36 indicates identity
Score > 18 indicates homology
MNLFHLQAPFQATGDQPQAIAQLVNSIEK + 2 Deamidated (NQ)
3598 753.7377 2258.1913 2258.1543 16.4 1 6 0.48 +1Score > 37 indicates identity
Score > 15 indicates homology
VVRGTDNDTTWLIISPNTQK + Deamidated (NQ)
3456 546.2866 2181.1173 2181.0922 11.5 1 6 1 +1Score > 38 indicates identity
Score > 18 indicates homology
KPLEQAFATMVSSLGNGMIR + 2 Oxidation (M)
3142 995.5167 1989.0188 1988.9924 13.3 1 6 0.98 +1Score > 39 indicates identity
Score > 18 indicates homology
NLTHCDFIALRNMVIR + Deamidated (NQ); Oxidation (M)
3011 946.4801 1890.9456 1890.9548 -4.83 1 6 0.83 +1Score > 40 indicates identity
Score > 17 indicates homology
QQGTGAVAHIQLAGESAPR + Deamidated (NQ)
546 354.7073 707.4000 707.3966 4.87 0 6 0.97 +1Score > 37 indicates identity
Score > 18 indicates homology
GAAAPPPK
4159 702.8953 2807.5521 2807.5605 -3.00 1 6 0.63 +1Score > 31 indicates identity
Score > 16 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
4147 699.8903 2795.5321 2795.5606 -10.2 0 6 0.49 +1Score > 32 indicates identity
Score > 15 indicates homology
SIQSGLIEPMVILLILVANATVGVVTE + Deamidated (NQ); Oxidation (M)
4392 633.3194 3161.5606 3161.5757 -4.76 0 6 0.46 +1Score > 36 indicates identity
Score > 15 indicates homology
LLGNVLVCVLAHHFGHDFNPQVQAAFQK + 3 Deamidated (NQ)
3469 730.0245 2187.0517 2187.0596 -3.63 1 6 0.69 +1Score > 39 indicates identity
Score > 16 indicates homology
LHFDSNNDVIYPLENLQR + Deamidated (NQ)
4577 718.7860 3588.8936 3588.8275 18.4 1 6 0.32 +1Score > 32 indicates identity
Score > 13 indicates homology
MAMLGNVLHLSPMVLATTTTTKPSLLNQSKCTK + 3 Deamidated (NQ)
3024 634.3524 1900.0354 1900.0054 15.8 1 6 0.35 +1Score > 37 indicates identity
Score > 14 indicates homology
QYNAVIPGIVQQKANAGK + 2 Deamidated (NQ)
3465 728.7313 2183.1721 2183.1685 1.63 0 6 0.54 +1Score > 36 indicates identity
Score > 15 indicates homology
ISLDSVLDSLLDTIDALPQR
4017 663.0620 2648.2189 2648.2581 -14.8 1 6 0.55 +1Score > 37 indicates identity
Score > 15 indicates homology
MPPSPLSSGAFYQRWLGPVLANDE + Deamidated (NQ); Oxidation (M)
2221 741.9474 1481.8802 1481.8540 17.7 1 6 0.91 +1Score > 32 indicates identity
Score > 18 indicates homology
MLFPSIIAGLKHR
3816 484.6611 2418.2691 2418.2583 4.45 1 6 0.79 +1Score > 37 indicates identity
Score > 17 indicates homology
NNIEYDFITLHVGLGTFLPVR + Deamidated (NQ)
4027 890.4855 2668.4347 2668.3934 15.5 0 6 0.67 +1Score > 34 indicates identity
Score > 16 indicates homology
LAVCVSLIGAANIPPQHLNLYQFK + 3 Deamidated (NQ)
3440 725.3625 2173.0657 2173.0871 -9.86 1 5 0.81 +1Score > 39 indicates identity
Score > 17 indicates homology
AIQGIEGVLQQMQATMQVAR + 2 Deamidated (NQ)
696 395.7094 789.4042 789.4055 -1.53 0 5 0.66 +1Score > 42 indicates identity
Score > 16 indicates homology
QAVNMVK + Deamidated (NQ)
3056 963.4778 1924.9410 1924.9162 12.9 0 5 0.5 +1Score > 40 indicates identity
Score > 15 indicates homology
MLTSLTDLCFSPIQVAE + Deamidated (NQ)
2465 798.4666 1594.9186 1594.8930 16.1 1 5 0.47 +1Score > 34 indicates identity
Score > 15 indicates homology
LLVDSGLNPVVLEAR + Deamidated (NQ)
2803 882.9743 1763.9340 1763.9458 -6.65 1 5 0.62 +1Score > 39 indicates identity
Score > 16 indicates homology
PFGGLFQLTLESTINK
1203 506.7425 1011.4704 1011.4695 0.92 1 5 0.76 +1Score > 40 indicates identity
Score > 17 indicates homology
MAVDIEYR + Oxidation (M)
1667 609.3926 1216.7706 1216.7584 10.1 0 5 0.96 +1Score > 23 indicates identity
Score > 18 indicates homology
IPLQLLVFFK
2572 546.6257 1636.8553 1636.8607 -3.30 1 5 0.5 +1Score > 39 indicates identity
Score > 15 indicates homology
MDVLLAPTVAEAVHR + Oxidation (M)
3130 662.0553 1983.1441 1983.1193 12.5 1 5 0.42 +1Score > 30 indicates identity
Score > 14 indicates homology
VPWGIVLLLGGGFALAKGSE
3352 1061.5780 2121.1414 2121.1504 -4.21 0 5 0.53 +1Score > 36 indicates identity
Score > 15 indicates homology
SLVVLFNSSGLLCSGTLINK
4032 669.3257 2673.2737 2673.3167 -16.1 1 5 0.55 +1Score > 38 indicates identity
Score > 15 indicates homology
AIFKSISSISNSTGSMGSSIGTSNIVE
2433 790.9658 1579.9170 1579.8861 19.6 0 5 0.94 +1Score > 33 indicates identity
Score > 18 indicates homology
LATLTQITPFFLSK + Deamidated (NQ)
2696 568.2866 1701.8380 1701.8495 -6.78 0 5 0.74 +1Score > 40 indicates identity
Score > 17 indicates homology
IAVGMGADVTVIDLSPE + Oxidation (M)
3546 744.3781 2230.1125 2230.1165 -1.79 0 5 0.66 +1Score > 39 indicates identity
Score > 16 indicates homology
VVAAQDHLTHVVNSVMQPQR + 2 Deamidated (NQ)
3708 1164.5986 2327.1826 2327.1645 7.81 1 5 0.35 +1Score > 38 indicates identity
Score > 13 indicates homology
LTLEAHNLIAADSSINFVGNVE + Deamidated (NQ)
3767 794.1042 2379.2908 2379.2885 0.94 0 5 1 +1Score > 34 indicates identity
Score > 18 indicates homology
FGIGADGVLFLAKPLHTSLHMR
3776 797.1479 2388.4219 2388.4041 7.45 0 5 1 +1Score > 24 indicates identity
Score > 18 indicates homology
ITIPLAVLIVICFMLAAVFIR + Oxidation (M)
2215 738.9471 1475.8796 1475.9088 -19.8 1 5 0.98 +1Score > 29 indicates identity
Score > 18 indicates homology
ARYPHLLIKPLR
2613 830.9258 1659.8370 1659.8250 7.23 1 5 0.57 +1Score > 41 indicates identity
Score > 15 indicates homology
MSTPVTRTQVPDGVR + Deamidated (NQ); Oxidation (M)
2859 451.2076 1800.8013 1800.7948 3.59 1 5 0.62 +1Score > 38 indicates identity
Score > 16 indicates homology
DGMKDGDAPADAQQLPR + Deamidated (NQ); Oxidation (M)
2714 570.9881 1709.9425 1709.9464 -2.30 1 5 0.47 +1Score > 37 indicates identity
Score > 15 indicates homology
FINEIILLSQINHR + Deamidated (NQ)
3209 677.0107 2028.0103 2028.0098 0.21 1 5 1 +1Score > 39 indicates identity
Score > 18 indicates homology
LYNMGRLTYLLDGDNVR + Oxidation (M)
3803 604.8379 2415.3225 2415.2879 14.3 1 5 0.41 +1Score > 33 indicates identity
Score > 14 indicates homology
SLHPHVPGVTNLQVMRAMTSLK
3851 822.1149 2463.3229 2463.3009 8.92 0 5 0.47 +1Score > 35 indicates identity
Score > 15 indicates homology
IAVAHYAAQLSTLDINPQPLSLE
2662 840.4528 1678.8910 1678.8824 5.12 1 5 0.92 +1Score > 39 indicates identity
Score > 17 indicates homology
LLSFNQKQMVAAASR + Oxidation (M)
2913 365.7983 1823.9551 1823.9312 13.1 1 5 0.88 +1Score > 38 indicates identity
Score > 17 indicates homology
GRINCNSNTTPIVHLK + Deamidated (NQ)
3817 807.7284 2420.1634 2420.1295 14.0 1 5 0.42 +1Score > 39 indicates identity
Score > 14 indicates homology
MVMIACQLKPHYCCLVPER + Oxidation (M)
3651 766.7673 2297.2801 2297.2929 -5.60 1 5 0.95 +1Score > 33 indicates identity
Score > 18 indicates homology
NLLAAVGAQQQMLKLTIWGVK + Oxidation (M)
3018 947.5063 1892.9980 1892.9666 16.6 0 5 0.88 +1Score > 38 indicates identity
Score > 17 indicates homology
ITGADVVVAVMSGNFIQR + Deamidated (NQ); Oxidation (M)
4288 746.9128 2983.6221 2983.6311 -3.01 1 5 1 +1Score > 31 indicates identity
Score > 18 indicates homology
LSSNTLVTTVMSNLGLHLAMRSVGVIVR + Oxidation (M)
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