MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2193 735.9225 1469.8304 1469.8276 1.96 1 6 1 +1Score > 37 indicates identity
Score > 19 indicates homology
AVNLNVPLVVRMK + 2 Deamidated (NQ); Oxidation (M)
2960 620.3224 1857.9454 1857.9459 -0.29 1 6 0.57 +1Score > 40 indicates identity
Score > 17 indicates homology
DAVLDDVLSLTVEDILE
1655 607.3388 1212.6630 1212.6601 2.40 1 6 0.53 +1Score > 40 indicates identity
Score > 16 indicates homology
KPAELVLSDLE
1853 649.3492 1296.6838 1296.6898 -4.60 1 6 0.7 +1Score > 39 indicates identity
Score > 17 indicates homology
NVTPNNNIRQK
2689 567.2935 1698.8587 1698.8546 2.42 1 6 0.93 +1Score > 40 indicates identity
Score > 19 indicates homology
RSVTSMALTVFQGMR + Oxidation (M)
3511 735.3856 2203.1350 2203.0943 18.4 0 6 0.98 +1Score > 38 indicates identity
Score > 19 indicates homology
VADQDVPPTSHPLPLTNVMR + Deamidated (NQ); Oxidation (M)
1978 455.2306 1362.6700 1362.6528 12.6 1 6 0.91 +1Score > 41 indicates identity
Score > 19 indicates homology
VGSAVGAAAGGEFDR
2314 509.6397 1525.8973 1525.8716 16.9 1 6 1 +1Score > 32 indicates identity
Score > 19 indicates homology
VVLSQVSLAISPGEK
1709 411.2422 1230.7048 1230.6867 14.7 1 6 0.95 +1Score > 37 indicates identity
Score > 19 indicates homology
RSIQMQTVLR
3279 520.5437 2078.1457 2078.1385 3.47 1 6 0.96 +1Score > 34 indicates identity
Score > 19 indicates homology
SHQLRVHSLALGHPIIGDK + Deamidated (NQ)
2231 745.9308 1489.8470 1489.8286 12.4 1 6 0.41 +1Score > 35 indicates identity
Score > 15 indicates homology
LINAMIGGSTKTLR + Oxidation (M)
129 403.2324 402.2251 402.2227 6.10 0 6 1 +1Score > 50 indicates identity
Score > 19 indicates homology
AGGAK
2939 615.6413 1843.9021 1843.8952 3.73 1 6 0.52 +1Score > 40 indicates identity
Score > 16 indicates homology
LVILDEADNLHGTYDR + Deamidated (NQ)
3303 524.7825 2095.1009 2095.0990 0.91 1 6 0.48 +1Score > 38 indicates identity
Score > 16 indicates homology
WTEFLNLLGDTVVLNSFK
436 334.2121 666.4096 666.4177 -12.1 1 6 0.76 +1Score > 29 indicates identity
Score > 18 indicates homology
IPGPKR
1676 610.8650 1219.7154 1219.7176 -1.77 0 6 0.76 +1Score > 34 indicates identity
Score > 18 indicates homology
LTTIYDIILR
1791 424.5555 1270.6447 1270.6643 -15.4 1 6 0.83 +1Score > 41 indicates identity
Score > 18 indicates homology
NRHAAAHIPQR + Deamidated (NQ)
2495 536.9345 1607.7817 1607.8042 -14.0 1 6 0.69 +1Score > 41 indicates identity
Score > 17 indicates homology
IKATYQDGTVIAQAE + Deamidated (NQ)
2889 603.6464 1807.9174 1807.9455 -15.5 1 6 0.93 +1Score > 40 indicates identity
Score > 19 indicates homology
QPIPLQVLLSQEAQLE + 3 Deamidated (NQ)
3411 718.3802 2152.1188 2152.1303 -5.38 1 6 0.75 +1Score > 38 indicates identity
Score > 18 indicates homology
LFGLAVLDITSGNFSVQEIK + 2 Deamidated (NQ)
4142 931.4904 2791.4494 2791.4942 -16.1 1 6 0.48 +1Score > 36 indicates identity
Score > 16 indicates homology
AAIKQAAQDIGIVFAANFSVGVNVMLK + Deamidated (NQ); Oxidation (M)
4545 687.1549 3430.7381 3430.7092 8.42 1 6 0.58 +1Score > 35 indicates identity
Score > 16 indicates homology
TVTIFTVGTGGMFDQRLVGATNQPKPAQWHR + 2 Deamidated (NQ); Oxidation (M)
4355 772.6244 3086.4685 3086.4689 -0.13 0 6 0.67 +1Score > 36 indicates identity
Score > 17 indicates homology
TMYDAQGIGLAASQVDHHIQLIVMDLSE + 2 Oxidation (M)
4394 1055.5140 3163.5202 3163.4703 15.8 1 6 0.93 +1Score > 36 indicates identity
Score > 19 indicates homology
QSEFLVCSGGGSYDSGAMALVVNHHGDIIK + Oxidation (M)
1574 589.8141 1177.6136 1177.6091 3.82 1 6 0.61 +1Score > 41 indicates identity
Score > 17 indicates homology
RGTAVTFLGQE
3085 648.3539 1942.0399 1942.0047 18.1 1 6 0.48 +1Score > 37 indicates identity
Score > 16 indicates homology
HKLLNGLDDIGITLQYE + Deamidated (NQ)
1654 607.3354 1212.6562 1212.6615 -4.33 0 6 1 +1Score > 40 indicates identity
Score > 19 indicates homology
VFSNDIHLLR
3015 631.6870 1892.0392 1892.0043 18.4 1 6 0.79 +1Score > 35 indicates identity
Score > 18 indicates homology
RLSTYVGNLNVVLFAPE + Deamidated (NQ)
3452 727.0833 2178.2281 2178.2082 9.13 0 6 0.93 +1Score > 32 indicates identity
Score > 18 indicates homology
CNLSTVPGLALAQLPALVALR + 2 Deamidated (NQ)
3471 547.7711 2187.0553 2187.0956 -18.4 1 6 0.57 +1Score > 39 indicates identity
Score > 16 indicates homology
DGSSKILFIYICPMQATVK + Deamidated (NQ); Oxidation (M)
1369 365.2094 1092.6064 1092.5928 12.5 1 6 0.84 +1Score > 37 indicates identity
Score > 18 indicates homology
RVGAVDVSYK
2233 746.3871 1490.7596 1490.7763 -11.1 1 6 1 +1Score > 41 indicates identity
Score > 19 indicates homology
RNTIDIITLGCSK + Deamidated (NQ)
2542 406.7163 1622.8361 1622.8450 -5.52 1 6 0.84 +1Score > 40 indicates identity
Score > 18 indicates homology
MSVLKQDGQFVVTR + Oxidation (M)
2583 548.9673 1643.8801 1643.8817 -1.01 1 6 0.83 +1Score > 39 indicates identity
Score > 18 indicates homology
WKDKPVAMSIVQAR + Oxidation (M)
3408 1076.5602 2151.1058 2151.0848 9.80 1 6 0.79 +1Score > 38 indicates identity
Score > 18 indicates homology
KPHTTTVAPPQINFIKNNE + 3 Deamidated (NQ)
3396 716.3818 2146.1236 2146.0874 16.8 1 6 0.88 +1Score > 38 indicates identity
Score > 18 indicates homology
RTALNLLMHLSGIATMTNR + 2 Deamidated (NQ); 2 Oxidation (M)
2144 720.4015 1438.7884 1438.8031 -10.2 1 6 0.86 +1Score > 38 indicates identity
Score > 18 indicates homology
ILQPLLEQGASLR + 2 Deamidated (NQ)
2810 884.4470 1766.8794 1766.8951 -8.88 0 6 0.92 +1Score > 40 indicates identity
Score > 18 indicates homology
DAPQGTVILLFNAHDR + Deamidated (NQ)
3617 758.4112 2272.2118 2272.2388 -11.9 1 6 0.75 +1Score > 36 indicates identity
Score > 18 indicates homology
KMLNVQSLDGLVPLLVAGFLE + Deamidated (NQ); Oxidation (M)
3710 777.4147 2329.2223 2329.1835 16.6 1 6 1 +1Score > 37 indicates identity
Score > 19 indicates homology
ARLDALTPAGMIAQIDLSITDE + Oxidation (M)
2118 712.8293 1423.6440 1423.6328 7.91 1 6 0.97 +1Score > 40 indicates identity
Score > 19 indicates homology
QRDDAVTDGGYAR + Deamidated (NQ)
2569 409.9789 1635.8865 1635.8944 -4.82 1 6 0.78 +1Score > 37 indicates identity
Score > 18 indicates homology
HIDQISNVLAQIKR + 2 Deamidated (NQ)
2815 885.0142 1768.0138 1767.9995 8.10 1 6 0.79 +1Score > 32 indicates identity
Score > 18 indicates homology
LNAASASFQPVRALPVK
3318 704.3696 2110.0870 2110.0921 -2.43 1 6 0.55 +1Score > 38 indicates identity
Score > 16 indicates homology
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
2928 919.4433 1836.8720 1836.8927 -11.3 1 6 0.8 +1Score > 40 indicates identity
Score > 18 indicates homology
NMNTSKFLALTPQLDE + Oxidation (M)
3458 728.0606 2181.1600 2181.1641 -1.88 1 6 0.8 +1Score > 37 indicates identity
Score > 18 indicates homology
ATTPLTAATSNPSTPAKPAKQK + Deamidated (NQ)
1471 379.8909 1136.6509 1136.6441 5.95 0 6 0.79 +1Score > 36 indicates identity
Score > 18 indicates homology
SIASTIASIFK
3698 773.4547 2317.3423 2317.3270 6.59 1 6 0.32 +1Score > 28 indicates identity
Score > 14 indicates homology
ILNIPKLGSVNVHGSILPQYR
2594 825.9337 1649.8528 1649.8739 -12.7 1 6 0.84 +1Score > 40 indicates identity
Score > 18 indicates homology
SLDTPMEFLPLFLK
2920 609.3378 1824.9916 1824.9655 14.3 1 6 0.94 +1Score > 37 indicates identity
Score > 18 indicates homology
ISLSNVMVDGVQLPPKE
3263 1038.5308 2075.0470 2075.0139 16.0 1 6 0.78 +1Score > 39 indicates identity
Score > 18 indicates homology
IAALQEMAQSGMNVSTGPVR + Oxidation (M)
3103 654.9877 1961.9413 1961.9233 9.15 1 6 0.95 +1Score > 40 indicates identity
Score > 18 indicates homology
AWLITFGTWMYSVNKE + Deamidated (NQ); Oxidation (M)
4231 724.8967 2895.5577 2895.5779 -6.99 0 6 0.9 +1Score > 33 indicates identity
Score > 18 indicates homology
LANLNADIMVVVAYGLLLPQAVLDTPR + Deamidated (NQ); Oxidation (M)
3967 645.8130 2579.2229 2579.2173 2.16 1 6 0.64 +1Score > 38 indicates identity
Score > 17 indicates homology
RIMDLADSGSGAVAANSSAAPVFLNE + Deamidated (NQ); Oxidation (M)
960 919.4965 918.4892 918.4957 -7.00 1 6 0.74 +1Score > 41 indicates identity
Score > 17 indicates homology
KNLQLMR + Deamidated (NQ); Oxidation (M)
1523 1159.7125 1158.7052 1158.6860 16.6 0 6 0.96 +1Score > 31 indicates identity
Score > 18 indicates homology
ILDTVISSIAK
3371 1068.5625 2135.1104 2135.0858 11.5 1 6 0.79 +1Score > 38 indicates identity
Score > 18 indicates homology
ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
2604 828.4850 1654.9554 1654.9519 2.17 1 6 0.4 +1Score > 33 indicates identity
Score > 15 indicates homology
RIIQVHSLVVKPHE + Deamidated (NQ)
2130 477.6046 1429.7920 1429.7929 -0.63 1 6 0.93 +1Score > 38 indicates identity
Score > 18 indicates homology
KNIPLNNGVYAVK + Deamidated (NQ)
3032 636.6174 1906.8304 1906.8037 14.0 1 6 0.94 +1Score > 37 indicates identity
Score > 18 indicates homology
NMVDPQNGKGSLCSQAAE + 2 Deamidated (NQ)
1845 647.3423 1292.6700 1292.6473 17.6 0 6 0.97 +1Score > 41 indicates identity
Score > 18 indicates homology
HLQANPANIASR + 2 Deamidated (NQ)
2720 429.2128 1712.8221 1712.8481 -15.2 1 6 0.66 +1Score > 40 indicates identity
Score > 17 indicates homology
LIQYHKISNNDPNR + 2 Deamidated (NQ)
3084 648.3373 1941.9901 1941.9778 6.33 0 6 0.8 +1Score > 39 indicates identity
Score > 18 indicates homology
QMATALAHCHQHGLVLR
2749 576.6675 1726.9807 1726.9505 17.5 1 6 0.72 +1Score > 35 indicates identity
Score > 17 indicates homology
ASDLKILPGLFGLSAPE
3510 735.3853 2203.1341 2203.1194 6.65 1 6 0.49 +1Score > 38 indicates identity
Score > 15 indicates homology
IMDYSLLLGIHVLNSNVRE + 2 Deamidated (NQ); Oxidation (M)
3554 746.0882 2235.2428 2235.2449 -0.96 1 6 0.84 +1Score > 33 indicates identity
Score > 18 indicates homology
LTPPPVKIAAMAHNLPVLQPE
3563 746.7275 2237.1607 2237.1587 0.90 1 6 1 +1Score > 38 indicates identity
Score > 19 indicates homology
GLTPSQIGVMLRDSHGVAQVR + Deamidated (NQ); Oxidation (M)
3016 947.4999 1892.9852 1892.9731 6.43 1 6 0.63 +1Score > 39 indicates identity
Score > 17 indicates homology
NFNVSVNILQGKISQTK + 4 Deamidated (NQ)
3632 761.4066 2281.1980 2281.1624 15.6 1 6 1 +1Score > 37 indicates identity
Score > 19 indicates homology
HQVLQSQGVLILQGSMLEVGE + Deamidated (NQ); Oxidation (M)
1476 380.2355 1137.6847 1137.6771 6.68 1 6 0.74 +1Score > 32 indicates identity
Score > 17 indicates homology
LGLPIAGRWR
2127 715.8731 1429.7316 1429.7412 -6.71 1 6 0.78 +1Score > 41 indicates identity
Score > 18 indicates homology
INTNGTLQTPNKK + 2 Deamidated (NQ)
3813 806.7614 2417.2624 2417.2161 19.1 1 6 0.97 +1Score > 37 indicates identity
Score > 18 indicates homology
QAALNAMPLVKPDQVRTPWHE + Deamidated (NQ); Oxidation (M)
2790 587.0005 1757.9797 1757.9676 6.88 1 6 0.98 +1Score > 35 indicates identity
Score > 18 indicates homology
NQVPFNAKGTVIVTIR + 2 Deamidated (NQ)
2681 848.9326 1695.8506 1695.8203 17.9 1 6 0.96 +1Score > 40 indicates identity
Score > 18 indicates homology
SQVEYAQSLAQTLQK + 3 Deamidated (NQ)
3195 674.3522 2020.0348 2020.0153 9.63 0 6 0.71 +1Score > 39 indicates identity
Score > 17 indicates homology
QVAFASTNIIPGLDFSPDK + Deamidated (NQ)
2901 908.9653 1815.9160 1815.9400 -13.2 1 6 0.87 +1Score > 40 indicates identity
Score > 18 indicates homology
VVTLDMLDLGAARANLE + Oxidation (M)
3990 873.0904 2616.2494 2616.2402 3.49 0 6 0.43 +1Score > 38 indicates identity
Score > 15 indicates homology
LSVQDQPSLSPTSLQNSSSHTTTAK + 3 Deamidated (NQ)
2455 397.7243 1586.8681 1586.8701 -1.29 1 6 0.89 +1Score > 38 indicates identity
Score > 18 indicates homology
MSILLIDLSGQELR
2490 402.7379 1606.9225 1606.9519 -18.3 1 6 0.88 +1Score > 33 indicates identity
Score > 18 indicates homology
VPGAGPRGSLTLTLLR
4041 672.0704 2684.2525 2684.2606 -3.02 1 6 1 +1Score > 37 indicates identity
Score > 19 indicates homology
KFISITVDGVTYNATPNTHSNFQE + 2 Deamidated (NQ)
2237 498.9250 1493.7532 1493.7586 -3.66 1 6 0.68 +1Score > 41 indicates identity
Score > 17 indicates homology
TGTLAPRQGNPEPR + Deamidated (NQ)
2104 710.3898 1418.7650 1418.7657 -0.43 1 6 0.71 +1Score > 40 indicates identity
Score > 17 indicates homology
NIGFKGSLINLIE + 2 Deamidated (NQ)
2861 601.2753 1800.8041 1800.8101 -3.35 1 6 0.59 +1Score > 38 indicates identity
Score > 16 indicates homology
TDFVARNDAFQDMVR + Deamidated (NQ); Oxidation (M)
3719 780.7208 2339.1406 2339.1604 -8.50 1 6 0.43 +1Score > 39 indicates identity
Score > 15 indicates homology
QNLLQAAGNVGQASGELLQQIGE + 2 Deamidated (NQ)
2066 701.9005 1401.7864 1401.8014 -10.6 1 6 0.86 +1Score > 37 indicates identity
Score > 18 indicates homology
TMLSRLVISPTGK
3267 692.7173 2075.1301 2075.0899 19.4 1 6 0.5 +1Score > 36 indicates identity
Score > 15 indicates homology
ATDLDVFLKGGAALDINSVR + Deamidated (NQ)
2517 538.3223 1611.9451 1611.9433 1.07 1 6 0.67 +1Score > 32 indicates identity
Score > 17 indicates homology
RPKPPGPQLGRPRR + Deamidated (NQ)
2740 862.4715 1722.9284 1722.9239 2.62 1 6 0.72 +1Score > 38 indicates identity
Score > 17 indicates homology
RPLKDISFGQFLMR + Oxidation (M)
777 835.4435 834.4362 834.4487 -15.0 0 6 0.3 +1Score > 42 indicates identity
Score > 13 indicates homology
AGVLLAYE
2494 804.8961 1607.7776 1607.7535 15.0 0 6 0.94 +1Score > 41 indicates identity
Score > 18 indicates homology
LDADLAGAMMGINGVK + Deamidated (NQ); 2 Oxidation (M)
3198 1012.0270 2022.0394 2022.0746 -17.4 1 6 0.59 +1Score > 39 indicates identity
Score > 16 indicates homology
LGGGPGAGDLQTLALEVAQQK
2962 465.7139 1858.8265 1858.8632 -19.7 1 6 0.94 +1Score > 38 indicates identity
Score > 18 indicates homology
SLMDHLGSGKNWDLNR + Deamidated (NQ); Oxidation (M)
3849 818.7513 2453.2321 2453.2261 2.44 1 6 0.6 +1Score > 38 indicates identity
Score > 16 indicates homology
GGADVIELGIPFSDPMADGPVIQR
2390 782.3890 1562.7634 1562.7763 -8.20 1 6 0.61 +1Score > 41 indicates identity
Score > 16 indicates homology
VNEAIPMPLGHGALE + Oxidation (M)
1896 440.2636 1317.7690 1317.7768 -5.96 1 6 0.88 +1Score > 33 indicates identity
Score > 18 indicates homology
LAAIKSLVNYAR
2595 825.9361 1649.8576 1649.8737 -9.70 1 6 0.87 +1Score > 40 indicates identity
Score > 18 indicates homology
DLIAANHVVEQTIAR + Deamidated (NQ)
3644 574.3112 2293.2157 2293.2165 -0.37 1 6 1 +1Score > 37 indicates identity
Score > 18 indicates homology
IDASQTPDLVPTLAVLGAVAEGR + Deamidated (NQ)
4058 898.4689 2692.3849 2692.3895 -1.71 1 6 0.38 +1Score > 37 indicates identity
Score > 14 indicates homology
TMGKIFGFLGLTTGVILHDLDSDAR + Oxidation (M)
4157 702.8949 2807.5505 2807.5605 -3.57 1 6 0.41 +1Score > 31 indicates identity
Score > 15 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
4158 702.8949 2807.5505 2807.5581 -2.69 0 6 0.95 +1Score > 31 indicates identity
Score > 18 indicates homology
SLGQFLLQILGMLSGVGIMLLIALYE + Oxidation (M)
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