MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
707 398.7157 795.4168 795.4014 19.4 0 29 0.4 +1Score > 39 indicates identity
Score > 38 indicates homology		 AVTTYLE
2009 688.8732 1375.7318 1375.7170 10.8 0 29 0.065 +1Score > 40 indicates identity
Score > 30 indicates homology		 DIMFGPIALGQSK
1998 457.5979 1369.7719 1369.7677 3.05 1 29 0.097 +1Score > 38 indicates identity
Score > 31 indicates homology		 NARLSILNAQLR + 2 Deamidated (NQ)
2080 470.5639 1408.6699 1408.6616 5.86 1 29 0.056 +1Score > 41 indicates identity
Score > 29 indicates homology		 SASGDNMSKIIDR + Oxidation (M)
808 424.7007 847.3868 847.3937 -8.04 1 29 0.12 +1Score > 42 indicates identity
Score > 32 indicates homology		 DDTWRR
948 457.7918 913.5690 913.5596 10.3 0 29 0.18 +1Score > 36 indicates identity
Score > 34 indicates homology		 ALLSQLLR + Deamidated (NQ)
869 437.7522 873.4898 873.4920 -2.42 0 29 0.6 +1Score > 42 indicates identity
Score > 39 indicates homology		 AQITTLAR + Deamidated (NQ)
571 364.2219 726.4292 726.4276 2.31 1 29 0.31 +1Score > 36 indicates identity IPQEIK
774 417.2499 832.4852 832.4840 1.45 0 29 0.3 +1Score > 38 indicates identity
Score > 36 indicates homology		 ITLAMLR + Oxidation (M)
1295 528.7958 1055.5770 1055.5723 4.47 1 29 0.74 +1Score > 40 indicates identity
Score > 40 indicates homology		 KLAAQPSDAR
929 452.2444 902.4742 902.4821 -8.74 0 29 0.44 +1Score > 43 indicates identity
Score > 38 indicates homology		 ISAADVATR
1697 614.8384 1227.6622 1227.6823 -16.3 0 29 0.5 +1Score > 40 indicates identity
Score > 38 indicates homology		 IDVGGSAAVLGAAK
378 315.1855 628.3564 628.3544 3.21 0 28 0.4 +1Score > 37 indicates identity
Score > 37 indicates homology		 LGGGTPK
2317 510.6183 1528.8331 1528.8072 16.9 0 28 0.092 +1Score > 39 indicates identity
Score > 30 indicates homology		 GGVMPIAPGYLAQVR + Deamidated (NQ)
1632 601.3411 1200.6676 1200.6601 6.25 1 28 0.1 +1Score > 40 indicates identity
Score > 31 indicates homology		 DLVENAISLVK + Deamidated (NQ)
484 343.2100 684.4054 684.3919 19.9 0 28 0.18 +1Score > 37 indicates identity
Score > 33 indicates homology		 ATAGIPR
907 894.4709 893.4636 893.4607 3.31 1 28 0.56 +1Score > 41 indicates identity
Score > 38 indicates homology		 GISEGLYR
589 366.7053 731.3960 731.3926 4.70 0 28 0.94 +1Score > 41 indicates identity
Score > 40 indicates homology		 TGTGQIR
217 473.3112 472.3039 472.3009 6.33 0 28 0.59 +1Score > 45 indicates identity
Score > 38 indicates homology		 IGGVK
403 322.2114 642.4082 642.4064 2.83 1 28 0.75 +1Score > 41 indicates identity
Score > 39 indicates homology		 IAKLAQ
1999 686.3422 1370.6698 1370.6929 -16.8 1 28 0.11 +1Score > 40 indicates identity
Score > 31 indicates homology		 LNLNLQDEAVIE + Deamidated (NQ)
1694 614.8380 1227.6614 1227.6710 -7.78 1 28 0.62 +1Score > 40 indicates identity
Score > 38 indicates homology		 NILNEAQIIAK + 2 Deamidated (NQ)
1137 494.2982 986.5818 986.6012 -19.6 1 28 0.71 +1Score > 39 indicates identity TILAETIVK
617 377.2180 752.4214 752.4181 4.48 0 28 0.13 +1Score > 38 indicates identity
Score > 31 indicates homology		 ALDVHAK
2079 470.5630 1408.6672 1408.6616 3.94 1 28 0.059 +1Score > 41 indicates identity
Score > 28 indicates homology		 SASGDNMSKIIDR + Oxidation (M)
345 307.7034 613.3922 613.3911 1.80 0 28 0.49 +1Score > 40 indicates identity
Score > 37 indicates homology		 IGGVLR
933 302.8481 905.5225 905.5083 15.7 1 27 0.61 +1Score > 38 indicates identity IRFTVNR + Deamidated (NQ)
388 631.3679 630.3606 630.3588 2.88 1 27 0.79 +1Score > 44 indicates identity
Score > 39 indicates homology		 QIEIK + Deamidated (NQ)
968 461.2588 920.5030 920.5113 -8.99 1 27 0.07 +1Score > 40 indicates identity
Score > 28 indicates homology		 IAVTSKMR + Oxidation (M)
1281 349.8420 1046.5042 1046.5178 -13.1 1 27 0.071 +1Score > 42 indicates identity
Score > 28 indicates homology		 KAQVQANMR + 2 Deamidated (NQ)
1194 337.5267 1009.5583 1009.5781 -19.6 1 27 0.07 +1Score > 37 indicates identity
Score > 28 indicates homology		 QRAAGIAPAR
844 430.7227 859.4308 859.4174 15.6 1 27 0.64 +1Score > 43 indicates identity
Score > 38 indicates homology		 IIQENIE + 2 Deamidated (NQ)
1631 601.3409 1200.6672 1200.6503 14.2 1 27 0.77 +1Score > 40 indicates identity
Score > 38 indicates homology		 SWALVDILER
1622 399.8964 1196.6674 1196.6877 -17.0 1 27 0.63 +1Score > 38 indicates identity
Score > 37 indicates homology		 DRIVTPLNIR + Deamidated (NQ)
270 544.3477 543.3404 543.3380 4.38 0 27 0.7 +1Score > 44 indicates identity
Score > 38 indicates homology		 IAGGVK
493 689.3964 688.3891 688.3868 3.36 0 27 0.65 +1Score > 43 indicates identity
Score > 38 indicates homology		 TGTVVGR
689 394.2215 786.4284 786.4235 6.26 0 27 0.058 +1Score > 42 indicates identity
Score > 27 indicates homology		 ANAQIIR + 2 Deamidated (NQ)
588 366.7052 731.3958 731.3926 4.43 0 27 0.78 +1Score > 44 indicates identity
Score > 38 indicates homology		 TGTGQIR
879 440.2538 878.4930 878.4861 7.87 0 27 0.39 +1Score > 37 indicates identity
Score > 35 indicates homology		 YILNSIR + Deamidated (NQ)
356 308.6877 615.3608 615.3704 -15.5 0 27 0.5 +1Score > 45 indicates identity
Score > 36 indicates homology		 AIVSAR
2516 538.3064 1611.8974 1611.8944 1.83 0 27 0.43 +1Score > 37 indicates identity
Score > 35 indicates homology		 DGGISGTVIAIAGVLNR
2051 699.8883 1397.7620 1397.7514 7.62 1 27 0.51 +1Score > 39 indicates identity
Score > 36 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
1388 553.3092 1104.6038 1104.5928 10.0 1 27 0.085 +1Score > 40 indicates identity
Score > 28 indicates homology		 QIELVFSGGR
637 381.2070 760.3994 760.3967 3.68 1 27 0.23 +1Score > 43 indicates identity
Score > 33 indicates homology		 AQTEALK + Deamidated (NQ)
398 320.1862 638.3578 638.3540 5.97 0 27 0.31 +1Score > 35 indicates identity
Score > 34 indicates homology		 FGIFR
411 322.7216 643.4286 643.4269 2.77 0 26 0.11 +1Score > 33 indicates identity
Score > 30 indicates homology		 LSVVVK
471 675.4062 674.3989 674.3963 3.91 0 26 0.7 +1Score > 39 indicates identity
Score > 37 indicates homology		 QVTISK
2101 472.5765 1414.7077 1414.7052 1.75 0 26 0.45 +1Score > 41 indicates identity
Score > 35 indicates homology		 TANAIVSQQTPQR + 2 Deamidated (NQ)
1857 434.2457 1299.7153 1299.7034 9.12 1 26 0.097 +1Score > 40 indicates identity
Score > 29 indicates homology		 QVGGLDEIILSR + Deamidated (NQ)
847 430.7479 859.4812 859.4651 18.8 0 26 0.47 +1Score > 42 indicates identity
Score > 36 indicates homology		 IVDSNIAK + Deamidated (NQ)
988 464.7131 927.4116 927.3974 15.4 1 26 0.25 +1Score > 38 indicates identity
Score > 33 indicates homology		 EFFDQNK + Deamidated (NQ)
532 352.2038 702.3930 702.4024 -13.3 1 26 0.24 +1Score > 44 indicates identity
Score > 33 indicates homology		 DTAKIR
1399 554.8010 1107.5874 1107.6077 -18.3 0 26 0.17 +1Score > 40 indicates identity
Score > 31 indicates homology		 FNVIIPSYR
1595 594.3206 1186.6266 1186.6081 15.6 1 26 0.27 +1Score > 41 indicates identity
Score > 33 indicates homology		 VNQELIQTLE + Deamidated (NQ)
800 423.2299 844.4452 844.4443 1.13 1 26 0.22 +1Score > 43 indicates identity
Score > 32 indicates homology		 IENGVWK
437 334.7089 667.4032 667.4017 2.29 0 26 0.094 +1Score > 30 indicates identity
Score > 28 indicates homology		 VPVPTR
1541 584.7751 1167.5356 1167.5482 -10.7 1 26 0.48 +1Score > 40 indicates identity
Score > 36 indicates homology		 VDAQVFEMTK + Deamidated (NQ)
2151 481.9529 1442.8369 1442.8093 19.1 1 26 0.15 +1Score > 35 indicates identity
Score > 30 indicates homology		 SASKNAIDLIGNLK
1215 339.4995 1015.4767 1015.4822 -5.42 1 26 0.095 +1Score > 40 indicates identity
Score > 28 indicates homology		 QPLNTGEQK + 2 Deamidated (NQ)
1861 651.3600 1300.7054 1300.6888 12.8 1 26 0.24 +1Score > 40 indicates identity
Score > 32 indicates homology		 ANIWLVRGSQR + 2 Deamidated (NQ)
255 516.3051 515.2978 515.2955 4.54 0 26 0.066 +1Score > 47 indicates identity
Score > 27 indicates homology		 ALAQL + Deamidated (NQ)
941 456.7321 911.4496 911.4461 3.90 1 26 0.1 +1Score > 40 indicates identity
Score > 29 indicates homology		 ESVIHDGR
628 380.2360 758.4574 758.4538 4.83 0 26 0.72 +1Score > 40 indicates identity
Score > 37 indicates homology		 IISDALK
1031 473.2502 944.4858 944.4814 4.66 1 26 0.3 +1Score > 43 indicates identity
Score > 33 indicates homology		 IDQLKAQE + Deamidated (NQ)
1176 501.2872 1000.5598 1000.5553 4.56 1 26 0.44 +1Score > 41 indicates identity
Score > 35 indicates homology		 QATLEQALK
1542 584.7753 1167.5360 1167.5482 -10.4 1 26 0.21 +1Score > 40 indicates identity
Score > 32 indicates homology		 VDAQVFEMTK + Deamidated (NQ)
771 416.2471 830.4796 830.4749 5.70 1 26 0.34 +1Score > 40 indicates identity
Score > 34 indicates homology		 TLEQIVK + Deamidated (NQ)
287 561.3054 560.2981 560.2918 11.2 0 26 0.4 +1Score > 44 indicates identity
Score > 35 indicates homology		 DATVR
329 603.3365 602.3292 602.3275 2.81 1 26 0.89 +1Score > 46 indicates identity
Score > 38 indicates homology		 EIDVK
1256 1035.5883 1034.5810 1034.6012 -19.5 1 26 0.48 +1Score > 38 indicates identity
Score > 35 indicates homology		 IEIYIVGTK
638 381.2082 760.4018 760.4153 -17.7 1 26 0.64 +1Score > 44 indicates identity
Score > 36 indicates homology		 AVMEALK
2687 566.6355 1696.8847 1696.8858 -0.68 1 26 0.11 +1Score > 39 indicates identity
Score > 29 indicates homology		 FGALLIIDEVMTGFR + Oxidation (M)
2369 778.4441 1554.8736 1554.8869 -8.50 1 26 0.41 +1Score > 37 indicates identity
Score > 35 indicates homology		 DKIIVQSVPDVTLK + Deamidated (NQ)
1428 374.5381 1120.5925 1120.5724 17.9 1 26 0.4 +1Score > 41 indicates identity
Score > 34 indicates homology		 TTNKNITSNK + Deamidated (NQ)
1039 316.5040 946.4902 946.4872 3.11 1 26 0.13 +1Score > 42 indicates identity
Score > 30 indicates homology		 FGIDPDKR
452 336.7027 671.3908 671.3827 12.1 1 26 0.26 +1Score > 42 indicates identity
Score > 32 indicates homology		 AGVGRGR
2199 491.9372 1472.7898 1472.8100 -13.7 0 26 1 +1Score > 40 indicates identity
Score > 38 indicates homology		 HGLPPTAGLGIGIDR
352 308.6756 615.3366 615.3452 -13.9 1 26 0.73 +1Score > 44 indicates identity
Score > 37 indicates homology		 AGNKAR
2370 778.4456 1554.8766 1554.8869 -6.57 1 26 0.44 +1Score > 36 indicates identity
Score > 35 indicates homology		 DKIIVQSVPDVTLK + Deamidated (NQ)
586 366.2202 730.4258 730.4337 -10.8 0 26 0.49 +1Score > 43 indicates identity
Score > 35 indicates homology		 VATTALR
1498 574.7867 1147.5588 1147.5469 10.4 1 26 0.92 +1Score > 42 indicates identity
Score > 38 indicates homology		 GESSGNLQTVR + Deamidated (NQ)
2160 726.3376 1450.6606 1450.6861 -17.6 1 26 0.43 +1Score > 40 indicates identity
Score > 34 indicates homology		 TTAEVMVAVDVTSE
1048 474.7680 947.5214 947.5110 11.0 0 25 0.63 +1Score > 40 indicates identity
Score > 36 indicates homology		 MGILSVQGK + Oxidation (M)
804 848.3922 847.3849 847.3937 -10.3 1 25 0.76 +1Score > 41 indicates identity
Score > 37 indicates homology		 DDTWRR
2052 699.8895 1397.7644 1397.7514 9.33 1 25 0.31 +1Score > 39 indicates identity
Score > 33 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
1634 301.4196 1201.6493 1201.6554 -5.09 1 25 0.73 +1Score > 41 indicates identity
Score > 36 indicates homology		 TLTLLDGNEVK
768 416.2207 830.4268 830.4134 16.2 1 25 0.69 +1Score > 44 indicates identity
Score > 36 indicates homology		 ELLDQGR + Deamidated (NQ)
770 416.2470 830.4794 830.4749 5.46 1 25 0.75 +1Score > 40 indicates identity
Score > 37 indicates homology		 TQVELLK + Deamidated (NQ)
536 352.2227 702.4308 702.4276 4.65 1 25 0.54 +1Score > 38 indicates identity
Score > 35 indicates homology		 KIDVTK
909 894.4714 893.4641 893.4607 3.87 1 25 0.88 +1Score > 41 indicates identity
Score > 37 indicates homology		 LSGELYGR
1171 500.2855 998.5564 998.5760 -19.6 1 25 0.43 +1Score > 40 indicates identity
Score > 34 indicates homology		 QIEAGAIGLK
1243 1031.5145 1030.5072 1030.5043 2.81 1 25 0.53 +1Score > 41 indicates identity
Score > 35 indicates homology		 TVQESPQSR
454 336.7121 671.4096 671.3966 19.4 1 25 0.81 +1Score > 44 indicates identity
Score > 37 indicates homology		 GVVEIR
286 561.3027 560.2954 560.2918 6.43 0 25 0.27 +1Score > 44 indicates identity
Score > 32 indicates homology		 DATVR
1617 399.8721 1196.5945 1196.6037 -7.71 0 25 0.58 +1Score > 40 indicates identity
Score > 35 indicates homology		 AINDAVIHTDK + Deamidated (NQ)
616 377.2175 752.4204 752.4181 3.15 0 25 0.11 +1Score > 38 indicates identity
Score > 28 indicates homology		 ALDVHAK
919 449.7426 897.4706 897.4668 4.26 0 25 0.34 +1Score > 39 indicates identity
Score > 33 indicates homology		 QQLTGHSK
1008 469.7329 937.4512 937.4505 0.78 1 25 0.12 +1Score > 41 indicates identity
Score > 29 indicates homology		 VFDSSVER
717 401.2336 800.4526 800.4392 16.8 0 25 0.37 +1Score > 43 indicates identity
Score > 33 indicates homology		 QLAVDGAK
662 386.7232 771.4318 771.4239 10.3 1 25 0.54 +1Score > 41 indicates identity
Score > 35 indicates homology		 IDRSGPK
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