MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2011 689.3800 1376.7454 1376.7313 10.3 1 7 0.87 +1Score > 39 indicates identity
Score > 19 indicates homology
SINALFSQRWR
2065 468.2635 1401.7687 1401.7867 -12.9 1 7 0.78 +1Score > 38 indicates identity
Score > 19 indicates homology
ESLHLIYSSILK
3534 741.3741 2221.1005 2221.1062 -2.59 1 7 0.91 +1Score > 39 indicates identity
Score > 19 indicates homology
TGFGTGGWQLSALNLCNKAAR
3578 562.0588 2244.2061 2244.2478 -18.6 0 7 0.38 +1Score > 36 indicates identity
Score > 16 indicates homology
SLGNVIDPLHVINGISLQDLK
1989 683.9164 1365.8182 1365.8119 4.66 1 7 0.79 +1Score > 31 indicates identity
Score > 19 indicates homology
GIVIIPLSAIELE
3064 966.0436 1930.0726 1930.0445 14.6 1 7 0.85 +1Score > 34 indicates identity
Score > 19 indicates homology
VQVGLVGDAQVGKTSLMVK + 2 Deamidated (NQ)
3071 645.3593 1933.0561 1933.0408 7.90 1 7 0.55 +1Score > 36 indicates identity
Score > 17 indicates homology
NEVLNTTVINGIGVYVVK + 2 Deamidated (NQ)
3000 471.7330 1882.9029 1882.9346 -16.8 1 7 0.71 +1Score > 40 indicates identity
Score > 18 indicates homology
TAQQNFIDSSMILLKR + 3 Deamidated (NQ); Oxidation (M)
2627 834.4504 1666.8862 1666.8665 11.8 0 7 0.61 +1Score > 38 indicates identity
Score > 18 indicates homology
SVPVNNLVQVVGTPLE + 3 Deamidated (NQ)
1824 640.8923 1279.7700 1279.7540 12.6 1 7 0.83 +1Score > 32 indicates identity
Score > 19 indicates homology
FQLLKYLLDK
2999 471.7327 1882.9017 1882.9207 -10.1 1 7 0.88 +1Score > 40 indicates identity
Score > 19 indicates homology
NIVFSVRNMSGITSADR + Deamidated (NQ); Oxidation (M)
2631 834.4722 1666.9298 1666.9083 12.9 0 7 0.6 +1Score > 36 indicates identity
Score > 17 indicates homology
LLINGGTISPFFFNK
1525 580.3619 1158.7092 1158.7125 -2.77 0 7 0.39 +1Score > 31 indicates identity
Score > 16 indicates homology
LLAIFLGSGLR
2881 602.9742 1805.9008 1805.9193 -10.2 1 7 0.99 +1Score > 41 indicates identity
Score > 20 indicates homology
TMLNAGADKVSINSAAVK + Deamidated (NQ); Oxidation (M)
3230 1020.5176 2039.0206 2039.0588 -18.7 1 7 0.81 +1Score > 39 indicates identity
Score > 19 indicates homology
EFYQAVVNHAQLTLHIR + Deamidated (NQ)
3785 600.3142 2397.2277 2397.2012 11.1 1 7 0.76 +1Score > 38 indicates identity
Score > 18 indicates homology
NNVPRPPTFQPFGSGPRACIGK + Deamidated (NQ)
4332 1015.8775 3044.6107 3044.5940 5.49 1 7 0.9 +1Score > 34 indicates identity
Score > 19 indicates homology
ALIGIVIGGFWSMSAATAMRLVPANDVPR + 2 Oxidation (M)
970 461.7313 921.4480 921.4304 19.1 0 7 0.9 +1Score > 42 indicates identity
Score > 19 indicates homology
LYGGAGNDR
2481 803.4626 1604.9106 1604.8926 11.2 0 7 0.31 +1Score > 34 indicates identity
Score > 14 indicates homology
IQLLYSQHSLYLK
1659 405.8963 1214.6671 1214.6507 13.5 1 7 0.93 +1Score > 40 indicates identity
Score > 19 indicates homology
QGSPLLTITRE + Deamidated (NQ)
3087 649.7085 1946.1037 1946.0771 13.6 1 7 0.38 +1Score > 32 indicates identity
Score > 15 indicates homology
SHVLVLMKGLPGGNASLPR + Deamidated (NQ)
843 860.4277 859.4204 859.4260 -6.52 1 7 0.52 +1Score > 42 indicates identity
Score > 17 indicates homology
DAGTAGGRR
3218 1018.0439 2034.0732 2034.0521 10.4 1 7 0.64 +1Score > 38 indicates identity
Score > 18 indicates homology
AFDIELIDQSAQSIVASVK + Deamidated (NQ)
476 341.6758 681.3370 681.3446 -11.0 0 7 0.43 +1Score > 38 indicates identity
Score > 16 indicates homology
IGSSYR
2361 517.6089 1549.8049 1549.7956 5.98 1 7 0.59 +1Score > 40 indicates identity
Score > 17 indicates homology
QGASNVAVIIKMMR + Deamidated (NQ); 2 Oxidation (M)
3207 507.7903 2027.1321 2027.1197 6.12 1 7 0.86 +1Score > 34 indicates identity
Score > 19 indicates homology
IIQANNATLAGVMIALNRK + Deamidated (NQ); Oxidation (M)
1581 591.3683 1180.7220 1180.7404 -15.5 1 7 0.99 +1Score > 31 indicates identity
Score > 19 indicates homology
QNLRLLVLGR
3125 991.4621 1980.9096 1980.9164 -3.41 1 7 0.64 +1Score > 39 indicates identity
Score > 18 indicates homology
VQNIGKTSIDDVNVSDFE + 2 Deamidated (NQ)
3489 731.7200 2192.1382 2192.1477 -4.35 1 7 0.24 +1Score > 38 indicates identity
Score > 13 indicates homology
QQFGRIINIASVNGLVGFAGK + 4 Deamidated (NQ)
4018 530.6513 2648.2201 2648.2581 -14.3 1 7 0.6 +1Score > 37 indicates identity
Score > 17 indicates homology
AHYQVDPAMFKPQADFLLRVNGE + 3 Deamidated (NQ)
1982 683.3259 1364.6372 1364.6554 -13.3 0 7 0.82 +1Score > 41 indicates identity
Score > 19 indicates homology
YWLYPYGTFR
3277 693.7116 2078.1130 2078.1120 0.48 0 7 0.47 +1Score > 37 indicates identity
Score > 16 indicates homology
LATALHSADLPLSALTSAGNR
2567 818.4420 1634.8694 1634.8372 19.8 1 7 0.67 +1Score > 39 indicates identity
Score > 18 indicates homology
LGVDMDKVMINIQK + 2 Oxidation (M)
4493 1106.8970 3317.6692 3317.6319 11.2 1 7 0.36 +1Score > 35 indicates identity
Score > 15 indicates homology
MAIATVVNNLGSTLGACGDINRNVMAPAAPFR + Deamidated (NQ); Oxidation (M)
2673 563.9988 1688.9746 1688.9495 14.9 1 7 0.4 +1Score > 32 indicates identity
Score > 15 indicates homology
LTLLQNVVSMKSTQK
1449 565.8312 1129.6478 1129.6343 12.0 1 7 0.92 +1Score > 38 indicates identity
Score > 19 indicates homology
LQETLGILSR + Deamidated (NQ)
1438 564.3257 1126.6368 1126.6458 -7.97 1 7 0.93 +1Score > 39 indicates identity
Score > 19 indicates homology
IQINKAQGQK
1339 360.2273 1077.6601 1077.6658 -5.33 1 7 0.38 +1Score > 30 indicates identity
Score > 15 indicates homology
AAKLLANHLK
2456 529.9635 1586.8687 1586.8780 -5.88 1 7 0.88 +1Score > 38 indicates identity
Score > 19 indicates homology
AQINTIAHKQIVLH + 2 Deamidated (NQ)
2684 849.4332 1696.8518 1696.8454 3.80 1 7 0.84 +1Score > 40 indicates identity
Score > 19 indicates homology
QQIQAYVMDISKTR + Deamidated (NQ); Oxidation (M)
2587 549.6504 1645.9294 1645.9039 15.5 1 7 0.91 +1Score > 35 indicates identity
Score > 19 indicates homology
VTTKYNVPLLINDR + Deamidated (NQ)
3245 514.2784 2053.0845 2053.1069 -10.9 1 7 0.65 +1Score > 38 indicates identity
Score > 18 indicates homology
QQISQLDGWQINGKIGIR
1704 410.5818 1228.7236 1228.7292 -4.56 1 7 0.67 +1Score > 36 indicates identity
Score > 18 indicates homology
RIFPAILSGQK
3081 648.0316 1941.0730 1941.0836 -5.47 0 7 0.68 +1Score > 35 indicates identity
Score > 18 indicates homology
AIILVSLPFNASWLNQR
3473 730.3733 2188.0981 2188.0800 8.25 1 7 0.49 +1Score > 39 indicates identity
Score > 16 indicates homology
SVYGQALYNSVNEQYNVLK
2950 618.9753 1853.9041 1853.9231 -10.3 0 7 0.88 +1Score > 40 indicates identity
Score > 19 indicates homology
TIDNTGKPTNVQANPQR + Deamidated (NQ)
1983 683.3268 1364.6390 1364.6242 10.9 1 7 0.91 +1Score > 41 indicates identity
Score > 19 indicates homology
VSAQLDRVMSDE + Oxidation (M)
2763 867.4499 1732.8852 1732.8665 10.8 1 7 0.95 +1Score > 40 indicates identity
Score > 19 indicates homology
VGSAVIIDSEITNCQK
3451 727.0816 2178.2230 2178.2082 6.79 0 7 0.99 +1Score > 32 indicates identity
Score > 19 indicates homology
CNLSTVPGLALAQLPALVALR + 2 Deamidated (NQ)
3477 730.3848 2188.1326 2188.1483 -7.18 1 7 0.72 +1Score > 38 indicates identity
Score > 18 indicates homology
NLSITNGLIVSEAVMMALAPK + Deamidated (NQ); Oxidation (M)
3917 842.4601 2524.3585 2524.3584 0.023 1 7 0.37 +1Score > 35 indicates identity
Score > 15 indicates homology
AIVTGHATIAGNPLVIAVMDSRFR + Oxidation (M)
4567 714.9721 3569.8241 3569.7560 19.1 1 7 0.25 +1Score > 34 indicates identity
Score > 13 indicates homology
AMVADNSVGDIESPVTPSTPGSPPVSPGPLSPGATPGK
2846 597.3180 1788.9322 1788.9258 3.58 1 7 0.81 +1Score > 40 indicates identity
Score > 18 indicates homology
VTFDLDANGILNVAAKE
2010 459.9141 1376.7205 1376.6936 19.5 1 7 0.92 +1Score > 40 indicates identity
Score > 19 indicates homology
INLAEQGYDLNK
2202 738.3596 1474.7046 1474.7012 2.36 1 7 0.53 +1Score > 41 indicates identity
Score > 17 indicates homology
NTEGILSGNATQNR + Deamidated (NQ)
2503 806.4191 1610.8236 1610.8264 -1.69 1 7 0.99 +1Score > 40 indicates identity
Score > 19 indicates homology
QINGPSDLARVSPQK + 2 Deamidated (NQ)
3768 794.7590 2381.2552 2381.2155 16.7 1 7 0.66 +1Score > 36 indicates identity
Score > 18 indicates homology
GTGLDVVWQPLLSVWLEDEPK + Deamidated (NQ)
3924 633.8301 2531.2913 2531.3206 -11.6 1 7 0.69 +1Score > 38 indicates identity
Score > 18 indicates homology
MTSHVLIATLAIPLPSAHPEFDR + Oxidation (M)
2005 458.8918 1373.6536 1373.6364 12.5 1 7 0.98 +1Score > 41 indicates identity
Score > 19 indicates homology
ENYIPDSFPHR
2440 527.9614 1580.8624 1580.8409 13.6 0 7 1 +1Score > 38 indicates identity
Score > 19 indicates homology
LSSLNPNLQNIPIR + 3 Deamidated (NQ)
4054 898.4501 2692.3285 2692.3391 -3.95 1 7 0.55 +1Score > 38 indicates identity
Score > 17 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
4368 778.9259 3111.6745 3111.6454 9.36 0 7 1 +1Score > 32 indicates identity
Score > 19 indicates homology
IVSSLVNNIIMPFVGVLLGGLDFSDLSFK + 2 Deamidated (NQ); Oxidation (M)
2315 509.9655 1526.8747 1526.8854 -7.05 1 7 0.87 +1Score > 35 indicates identity
Score > 19 indicates homology
VGLNPVKINTVVMK + Oxidation (M)
3249 515.0069 2055.9985 2056.0009 -1.16 1 7 0.69 +1Score > 40 indicates identity
Score > 18 indicates homology
SQNMLNKLNICFLFAPK + 3 Deamidated (NQ); Oxidation (M)
4160 702.8966 2807.5573 2807.5329 8.69 1 7 0.43 +1Score > 31 indicates identity
Score > 16 indicates homology
MMLEHVLVLSAYLLSIGIYGLITSR + Oxidation (M)
2865 901.4946 1800.9746 1800.9444 16.8 1 7 0.97 +1Score > 38 indicates identity
Score > 19 indicates homology
VLVLCLDQSYPPELR
4052 898.4482 2692.3228 2692.2724 18.7 1 7 0.72 +1Score > 38 indicates identity
Score > 18 indicates homology
MANSFAARIFTTLSDLQTNMANLK + 3 Deamidated (NQ); 2 Oxidation (M)
2876 601.6680 1801.9822 1801.9673 8.25 1 7 0.8 +1Score > 37 indicates identity
Score > 18 indicates homology
DAIKDDALVISTLVDSK
3089 650.9976 1949.9710 1949.9694 0.81 0 7 0.91 +1Score > 40 indicates identity
Score > 19 indicates homology
ALTAGNTGLTLNIAANYGGR + 3 Deamidated (NQ)
3957 856.4682 2566.3828 2566.3326 19.6 1 7 0.69 +1Score > 34 indicates identity
Score > 18 indicates homology
ISLMRNHSNIQSVSNVPYHLLK + Deamidated (NQ); Oxidation (M)
2716 571.3289 1710.9649 1710.9628 1.20 1 7 0.86 +1Score > 35 indicates identity
Score > 19 indicates homology
AQSLQLSLGDSPLKVR
2561 327.1988 1630.9576 1630.9294 17.3 0 7 0.98 +1Score > 31 indicates identity
Score > 19 indicates homology
IQIAISLNGGYIQIK + Deamidated (NQ)
3027 636.2832 1905.8278 1905.8488 -11.0 0 7 0.44 +1Score > 36 indicates identity
Score > 16 indicates homology
TLQDMMNIIDYNQYK + Deamidated (NQ); Oxidation (M)
3306 699.6874 2096.0404 2096.0347 2.71 0 7 0.52 +1Score > 39 indicates identity
Score > 16 indicates homology
IINADAMQIYSGLDVLTNK + 2 Deamidated (NQ); Oxidation (M)
3772 796.0934 2385.2584 2385.2284 12.6 0 7 0.59 +1Score > 37 indicates identity
Score > 17 indicates homology
TGVDMLPATVATLAQNPMIQGIK + Deamidated (NQ); Oxidation (M)
2529 538.9871 1613.9395 1613.9141 15.7 0 7 0.92 +1Score > 32 indicates identity
Score > 19 indicates homology
LIGQQGLVDGVFLVR + Deamidated (NQ)
2450 794.4044 1586.7942 1586.7787 9.77 1 7 0.71 +1Score > 40 indicates identity
Score > 18 indicates homology
ASPSKGNIDITVNAAE + Deamidated (NQ)
3262 1038.5305 2075.0464 2075.0647 -8.79 1 7 0.86 +1Score > 39 indicates identity
Score > 19 indicates homology
AAAKGSDLFLIVGAPNSSNSR + Deamidated (NQ)
3672 1154.0570 2306.0994 2306.1147 -6.63 1 7 0.36 +1Score > 39 indicates identity
Score > 15 indicates homology
HQPDIVICVGQAGGRMQITPE + Deamidated (NQ)
3845 818.4251 2452.2535 2452.2169 14.9 1 7 0.42 +1Score > 38 indicates identity
Score > 15 indicates homology
HAIAQGVNPVTAFQMATLNPAER + Deamidated (NQ); Oxidation (M)
566 723.3635 722.3562 722.3520 5.80 0 7 0.83 +1Score > 41 indicates identity
Score > 18 indicates homology
TITMIE + Oxidation (M)
1470 379.8907 1136.6503 1136.6302 17.7 1 7 0.87 +1Score > 36 indicates identity
Score > 19 indicates homology
LNHLIGSVER
3190 673.6752 2018.0038 2017.9714 16.1 1 7 0.62 +1Score > 40 indicates identity
Score > 17 indicates homology
MFKMVSSPHTHGTNLTAK + 2 Oxidation (M)
4499 1111.9369 3332.7889 3332.8306 -12.5 1 7 0.58 +1Score > 32 indicates identity
Score > 17 indicates homology
TLVMALGAIGAALFYGDGVITPALSVLSAVEGLK + Oxidation (M)
2311 760.9150 1519.8154 1519.8068 5.66 0 7 0.67 +1Score > 39 indicates identity
Score > 17 indicates homology
LLAFPTQTSLACAK
2932 919.9504 1837.8862 1837.8734 7.00 1 7 0.92 +1Score > 40 indicates identity
Score > 19 indicates homology
LENAGVNFDYLGAVNIE + Deamidated (NQ)
4241 970.4946 2908.4620 2908.4939 -11.0 1 7 1 +1Score > 37 indicates identity
Score > 19 indicates homology
IQAISLPMIMTPPHKHLIAQAHNGSGK + 3 Deamidated (NQ); Oxidation (M)
218 474.2697 473.2624 473.2598 5.58 0 7 0.77 +1Score > 47 indicates identity
Score > 18 indicates homology
AGAAGK
2543 812.4304 1622.8462 1622.8304 9.75 1 7 0.97 +1Score > 40 indicates identity
Score > 19 indicates homology
LAGDSFIVSSEFPVR
3297 698.0425 2091.1057 2091.1364 -14.7 1 7 0.7 +1Score > 37 indicates identity
Score > 18 indicates homology
FPIQVILGSATPSLNSYKR + Deamidated (NQ)
2378 520.3176 1557.9310 1557.9242 4.32 1 7 0.77 +1Score > 29 indicates identity
Score > 18 indicates homology
DVTLFIINLKNLR
3162 1001.5388 2001.0630 2001.0677 -2.32 1 7 0.67 +1Score > 39 indicates identity
Score > 17 indicates homology
VIGMLQNITRNLITQDR + Deamidated (NQ); Oxidation (M)
3347 707.3846 2119.1320 2119.1426 -5.01 0 7 0.69 +1Score > 37 indicates identity
Score > 17 indicates homology
QVVVADVHAVQEPAPIPPPR + Deamidated (NQ)
1930 666.8862 1331.7578 1331.7561 1.30 1 7 0.73 +1Score > 36 indicates identity
Score > 18 indicates homology
ALYGGDIVLQKR
4333 763.4014 3049.5765 3049.5244 17.1 1 7 1 +1Score > 35 indicates identity
Score > 19 indicates homology
IATGEYDALGDISTLADPGVVQHLIDTHK + Deamidated (NQ)
2123 714.4257 1426.8368 1426.8143 15.8 1 6 0.51 +1Score > 34 indicates identity
Score > 16 indicates homology
IQQLAVSGLKVNR + 2 Deamidated (NQ)
2670 843.9459 1685.8772 1685.8631 8.38 1 6 0.45 +1Score > 40 indicates identity
Score > 16 indicates homology
QCLRNNTPNSLTLR
2994 471.0006 1879.9733 1879.9713 1.05 1 6 0.84 +1Score > 39 indicates identity
Score > 18 indicates homology
NEGITALYSGLKPTMIR + Deamidated (NQ); Oxidation (M)
3871 620.8225 2479.2609 2479.2929 -12.9 0 6 0.8 +1Score > 38 indicates identity
Score > 18 indicates homology
LTMVVLNGMIGFAVTLYFIAMK + 3 Oxidation (M)
1502 384.2172 1149.6298 1149.6367 -6.00 1 6 0.66 +1Score > 39 indicates identity
Score > 17 indicates homology
QGARQGPPALR
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