MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2952 928.4583 1854.9020 1854.9224 -11.0 0 8 0.96 +1Score > 40 indicates identity
Score > 20 indicates homology
GANASAPDQLSLALAWNR + Deamidated (NQ)
4415 1061.5273 3181.5601 3181.5682 -2.55 1 8 0.25 +1Score > 36 indicates identity
Score > 14 indicates homology
LAMHGTHNMLNSLAAIGVATQMGIADDKIR + Deamidated (NQ); 2 Oxidation (M)
652 384.7069 767.3992 767.4038 -5.96 0 8 0.49 +1Score > 37 indicates identity
Score > 17 indicates homology
IASHGQR
2818 591.6478 1771.9216 1771.9250 -1.96 1 8 0.54 +1Score > 39 indicates identity
Score > 18 indicates homology
VNVLRLNMALDAAGATQ + Oxidation (M)
2012 460.5603 1378.6591 1378.6364 16.4 1 8 0.97 +1Score > 41 indicates identity
Score > 20 indicates homology
NLDSIEDANFNK
2376 312.3888 1556.9076 1556.8787 18.6 1 8 0.89 +1Score > 33 indicates identity
Score > 20 indicates homology
NPLARGIDLPHVVR + Deamidated (NQ)
3220 679.3964 2035.1674 2035.1605 3.38 1 8 0.23 +1Score > 30 indicates identity
Score > 14 indicates homology
VQLVLPLLVAEAAAAPAFLE + Deamidated (NQ)
3241 682.7174 2045.1304 2045.1044 12.7 1 8 0.44 +1Score > 34 indicates identity
Score > 17 indicates homology
LAKNGAAFISVVSSNPDLLK + 2 Deamidated (NQ)
3604 1134.0784 2266.1422 2266.1805 -16.9 1 8 0.29 +1Score > 39 indicates identity
Score > 15 indicates homology
NPIQDIKITSDTNVPVNAGIR + 2 Deamidated (NQ)
3910 631.0999 2520.3705 2520.3295 16.3 1 8 0.48 +1Score > 34 indicates identity
Score > 17 indicates homology
ALNPNNQTQAINALIANAKNGLVR + 3 Deamidated (NQ)
3670 769.3719 2305.0939 2305.1235 -12.9 1 8 0.79 +1Score > 39 indicates identity
Score > 19 indicates homology
GDLDWVRPTPNIPKMPPMPE + Oxidation (M)
3912 841.7895 2522.3467 2522.3268 7.88 1 8 0.6 +1Score > 35 indicates identity
Score > 18 indicates homology
FLGVDEQVQLADLLPRPALAQVE + 2 Deamidated (NQ)
1351 362.5461 1084.6165 1084.5989 16.2 1 8 0.9 +1Score > 40 indicates identity
Score > 20 indicates homology
NGLRLQQVR + 2 Deamidated (NQ)
3492 1098.5807 2195.1468 2195.1330 6.33 1 8 0.2 +1Score > 38 indicates identity
Score > 13 indicates homology
MKSASIPIYLQQAIMNTLR + 2 Deamidated (NQ); Oxidation (M)
2092 706.4304 1410.8462 1410.8194 19.0 1 8 0.65 +1Score > 33 indicates identity
Score > 18 indicates homology
KNVLILGAGGQIAR + 2 Deamidated (NQ)
2961 620.6158 1858.8256 1858.8605 -18.8 1 8 0.82 +1Score > 38 indicates identity
Score > 19 indicates homology
NGRPRSNSWQGNMGGNK
3841 816.0844 2445.2314 2445.2798 -19.8 1 8 0.38 +1Score > 38 indicates identity
Score > 16 indicates homology
LQPGIYKNMGSNSVNIIINNTR
1105 324.8395 971.4967 971.5011 -4.54 1 8 0.96 +1Score > 41 indicates identity
Score > 20 indicates homology
KMWHINK + Oxidation (M)
2205 738.4058 1474.7970 1474.7780 12.9 0 8 0.82 +1Score > 39 indicates identity
Score > 19 indicates homology
LWLTTLGNSGANTK
2656 837.4734 1672.9322 1672.9624 -18.0 1 8 0.27 +1Score > 36 indicates identity
Score > 15 indicates homology
LSIYVPALNRTVTAR
3906 838.4314 2512.2724 2512.2849 -5.00 1 8 0.76 +1Score > 38 indicates identity
Score > 19 indicates homology
WLDKQEPSSVIYISFGTTTALR + Deamidated (NQ)
2169 729.9164 1457.8182 1457.8136 3.16 1 8 0.39 +1Score > 37 indicates identity
Score > 16 indicates homology
AVQSAMRLGALGIR + Oxidation (M)
2910 365.5974 1822.9506 1822.9173 18.3 0 8 0.69 +1Score > 39 indicates identity
Score > 19 indicates homology
TLIHQLNAQGDQNLTR + 2 Deamidated (NQ)
3840 816.0719 2445.1939 2445.2362 -17.3 0 8 0.36 +1Score > 39 indicates identity
Score > 16 indicates homology
TWMQPGLAVILAGDNPASSVYVR + Deamidated (NQ)
2466 532.9446 1595.8120 1595.8051 4.29 1 8 0.87 +1Score > 40 indicates identity
Score > 20 indicates homology
VAIMKGLTVQFMNE + Oxidation (M)
3585 750.0927 2247.2563 2247.2362 8.94 1 8 0.38 +1Score > 32 indicates identity
Score > 16 indicates homology
QLLASSLFVGLGSESTINLLGK + Deamidated (NQ)
1660 405.8966 1214.6680 1214.6507 14.3 0 8 0.77 +1Score > 40 indicates identity
Score > 19 indicates homology
ALIAGNIGTVASE
2629 834.4564 1666.8982 1666.9004 -1.29 0 8 0.89 +1Score > 38 indicates identity
Score > 20 indicates homology
TSIQMLAIFSLLWK + Deamidated (NQ); Oxidation (M)
2655 837.4726 1672.9306 1672.9069 14.2 1 8 0.47 +1Score > 36 indicates identity
Score > 17 indicates homology
KLVILGGAMQGNVLDK + 2 Deamidated (NQ); Oxidation (M)
699 792.4238 791.4165 791.4025 17.7 1 8 0.35 +1Score > 42 indicates identity
Score > 16 indicates homology
TVEQSTK
1864 434.9121 1301.7145 1301.7303 -12.2 1 8 0.97 +1Score > 40 indicates identity
Score > 20 indicates homology
VVRILAGSTDGSK
3124 991.4589 1980.9032 1980.9389 -18.0 1 8 0.92 +1Score > 38 indicates identity
Score > 20 indicates homology
GTDKLVNDFNSSINVDSR + Deamidated (NQ)
3289 696.3799 2086.1179 2086.0793 18.5 1 8 0.36 +1Score > 37 indicates identity
Score > 16 indicates homology
LTTLNATIQQQDQELAGLK + 2 Deamidated (NQ)
2782 876.4321 1750.8496 1750.8573 -4.37 1 8 0.85 +1Score > 41 indicates identity
Score > 19 indicates homology
ARFHDAMNILGPQHK + Deamidated (NQ); Oxidation (M)
3355 709.7245 2126.1517 2126.1736 -10.3 1 8 0.84 +1Score > 36 indicates identity
Score > 19 indicates homology
LFVDTLLTVQPLQNQEIR
2281 378.7177 1510.8417 1510.8177 15.9 0 8 0.87 +1Score > 37 indicates identity
Score > 20 indicates homology
NVMLPQLIAGVSPR + Deamidated (NQ); Oxidation (M)
2301 758.3746 1514.7346 1514.7253 6.18 0 8 0.58 +1Score > 41 indicates identity
Score > 18 indicates homology
NTFSATSPPLLTHE + Deamidated (NQ)
2112 474.8926 1421.6560 1421.6643 -5.83 0 8 0.73 +1Score > 41 indicates identity
Score > 19 indicates homology
MQDLNLAMDALR + 2 Oxidation (M)
2919 913.4852 1824.9558 1824.9556 0.13 1 8 0.7 +1Score > 39 indicates identity
Score > 19 indicates homology
TFYQIVKSLARPMDR + Deamidated (NQ)
575 364.7019 727.3892 727.3864 3.85 0 8 0.91 +1Score > 39 indicates identity
Score > 20 indicates homology
SPATPGAK
3030 954.4216 1906.8286 1906.8328 -2.19 0 8 0.36 +1Score > 36 indicates identity
Score > 16 indicates homology
TLQDMMNIIDYNQYK + 2 Deamidated (NQ); Oxidation (M)
3780 599.2966 2393.1573 2393.1877 -12.7 0 8 0.3 +1Score > 39 indicates identity
Score > 15 indicates homology
SHNHPSFIEPYQGAGTGVGGILR
2485 536.3143 1605.9211 1605.9130 5.02 1 8 0.69 +1Score > 33 indicates identity
Score > 19 indicates homology
GIIQIYVSPVFKNK + Deamidated (NQ)
2825 889.4637 1776.9128 1776.9258 -7.28 1 8 0.84 +1Score > 40 indicates identity
Score > 19 indicates homology
VTGQEVPFSSSASQLLK
3330 1058.5403 2115.0660 2115.0524 6.45 1 8 0.66 +1Score > 39 indicates identity
Score > 18 indicates homology
GSNLEFALGYSHPVLITAPE + Deamidated (NQ)
1980 455.2483 1362.7231 1362.7143 6.44 1 8 0.62 +1Score > 40 indicates identity
Score > 18 indicates homology
SKTNSIFQLAPR + 2 Deamidated (NQ)
3728 783.4315 2347.2727 2347.2933 -8.80 1 8 0.3 +1Score > 35 indicates identity
Score > 15 indicates homology
AQEPVKGPVSTKPGSCPIILIR + Deamidated (NQ)
4600 921.5124 3682.0205 3681.9539 18.1 1 8 1 +1Score > 29 indicates identity
Score > 20 indicates homology
AISVPGDISSAAFLIAAVMVVPGSSLLIENVGINPSR + 2 Deamidated (NQ); Oxidation (M)
2650 558.3142 1671.9208 1671.9235 -1.65 1 8 0.55 +1Score > 37 indicates identity
Score > 18 indicates homology
YVAYNAYKLIVLNK + Deamidated (NQ)
2975 623.3232 1866.9478 1866.9377 5.42 0 8 0.99 +1Score > 40 indicates identity
Score > 20 indicates homology
AYLVQAFTHASYINNR
1965 451.9028 1352.6866 1352.6758 7.94 1 8 0.79 +1Score > 40 indicates identity
Score > 19 indicates homology
DATFSPAIMTKR + Oxidation (M)
3002 943.0084 1884.0022 1883.9999 1.23 1 8 0.89 +1Score > 38 indicates identity
Score > 20 indicates homology
NQVNQDLMIAGIRSGLR
3037 957.4919 1912.9692 1912.9869 -9.23 1 8 0.8 +1Score > 40 indicates identity
Score > 19 indicates homology
LPLMELLWQAQYVHR + Deamidated (NQ); Oxidation (M)
2271 753.9350 1505.8554 1505.8314 16.0 1 8 0.44 +1Score > 35 indicates identity
Score > 17 indicates homology
LLEPSLGKSGVNHR
2584 822.9483 1643.8820 1643.8705 7.03 1 8 0.65 +1Score > 39 indicates identity
Score > 18 indicates homology
KLGFPTANLCLPADK
2741 862.9909 1723.9672 1723.9608 3.77 0 8 0.39 +1Score > 35 indicates identity
Score > 16 indicates homology
LLVLDEPTQGIDIIGK + Deamidated (NQ)
3385 1071.5735 2141.1324 2141.1553 -10.7 1 8 0.83 +1Score > 37 indicates identity
Score > 19 indicates homology
QVDVLLVVGATNSSNSNRLR
2326 511.2901 1530.8485 1530.8518 -2.16 1 8 0.61 +1Score > 38 indicates identity
Score > 18 indicates homology
TYASLLPRNNLIR + Deamidated (NQ)
4171 705.8503 2819.3721 2819.3324 14.1 0 8 0.4 +1Score > 37 indicates identity
Score > 16 indicates homology
AQVSVDMFLFSSQYQDVASLSNLPR + 2 Deamidated (NQ); Oxidation (M)
2412 394.2335 1572.9049 1572.8736 19.9 1 8 0.25 +1Score > 34 indicates identity
Score > 14 indicates homology
AVLLGFNVRADTAAR
2765 578.6564 1732.9474 1732.9359 6.59 1 8 0.74 +1Score > 37 indicates identity
Score > 19 indicates homology
DVSLSIASGEIFGIIGR
3599 754.3941 2260.1605 2260.1409 8.64 1 8 0.27 +1Score > 38 indicates identity
Score > 14 indicates homology
IFTYSKLGTIAGSMQDTGVVR + Deamidated (NQ); Oxidation (M)
1554 391.2053 1170.5941 1170.5815 10.7 0 7 0.93 +1Score > 40 indicates identity
Score > 20 indicates homology
IASMHSQQIR + Deamidated (NQ)
2747 576.6489 1726.9249 1726.9287 -2.24 1 7 0.7 +1Score > 38 indicates identity
Score > 18 indicates homology
QVLEPLMNLEVTVAR + Oxidation (M)
2978 934.5607 1867.1068 1867.0713 19.0 1 7 0.49 +1Score > 27 indicates identity
Score > 17 indicates homology
LRQGLTMLPQLLVNVR + Deamidated (NQ); Oxidation (M)
3496 1099.5430 2197.0714 2197.0538 8.01 0 7 0.82 +1Score > 39 indicates identity
Score > 19 indicates homology
ASGNHNLILSSYGINTVGSYK + 3 Deamidated (NQ)
787 842.4392 841.4319 841.4406 -10.3 1 7 0.98 +1Score > 39 indicates identity
Score > 20 indicates homology
RINADPR + Deamidated (NQ)
1350 543.2889 1084.5632 1084.5441 17.7 0 7 0.99 +1Score > 40 indicates identity
Score > 20 indicates homology
LFSIDNFTK + Deamidated (NQ)
2755 865.4790 1728.9434 1728.9154 16.2 0 7 0.47 +1Score > 38 indicates identity
Score > 17 indicates homology
VTMINAIPMASLDPIK + Oxidation (M)
1169 333.8355 998.4847 998.5032 -18.6 1 7 0.77 +1Score > 40 indicates identity
Score > 19 indicates homology
KSLHLTNGE + Deamidated (NQ)
2398 783.9711 1565.9276 1565.9215 3.95 0 7 0.63 +1Score > 31 indicates identity
Score > 18 indicates homology
SPALPLGIAMGLLLGK + Oxidation (M)
3328 529.5411 2114.1353 2114.1081 12.9 0 7 0.34 +1Score > 37 indicates identity
Score > 15 indicates homology
QLLAWQAQIQSIMAILGAR + 4 Deamidated (NQ)
3605 1134.0791 2266.1436 2266.1805 -16.2 1 7 0.28 +1Score > 39 indicates identity
Score > 14 indicates homology
NPIQDIKITSDTNVPVNAGIR + 2 Deamidated (NQ)
2306 759.9402 1517.8658 1517.8454 13.5 0 7 0.89 +1Score > 34 indicates identity
Score > 19 indicates homology
SILGNLVNVTDVFK
3846 818.4258 2452.2556 2452.2169 15.8 1 7 0.49 +1Score > 38 indicates identity
Score > 17 indicates homology
HAIAQGVNPVTAFQMATLNPAER + Deamidated (NQ); Oxidation (M)
1988 683.8631 1365.7116 1365.7292 -12.9 0 7 0.64 +1Score > 40 indicates identity
Score > 18 indicates homology
IAAQDYIIGVFR + Deamidated (NQ)
2598 826.4328 1650.8510 1650.8213 18.0 1 7 0.36 +1Score > 40 indicates identity
Score > 15 indicates homology
AIDADEVQQIAHLAR + 2 Deamidated (NQ)
3494 733.3633 2197.0681 2197.0386 13.4 1 7 0.96 +1Score > 39 indicates identity
Score > 20 indicates homology
SSRNASISNGTSISDTIFPIE + 2 Deamidated (NQ)
3639 765.3926 2293.1560 2293.1552 0.36 1 7 0.81 +1Score > 38 indicates identity
Score > 19 indicates homology
MLTPEIQYLSDLGANLWVTK + 2 Deamidated (NQ)
3713 778.1046 2331.2920 2331.2726 8.31 1 7 0.62 +1Score > 33 indicates identity
Score > 18 indicates homology
DPLQVLPPTVVDQIKLWQLE + Deamidated (NQ)
3299 698.3595 2092.0567 2092.0775 -9.98 1 7 0.94 +1Score > 39 indicates identity
Score > 20 indicates homology
GGAIWLLTGPNMGGKSTFLR + Deamidated (NQ); Oxidation (M)
3752 790.4220 2368.2442 2368.2784 -14.5 1 7 0.87 +1Score > 37 indicates identity
Score > 19 indicates homology
MQSLTLQPISRINGTINLPGSK + Deamidated (NQ)
3809 484.4539 2417.2331 2417.1976 14.7 1 7 0.47 +1Score > 38 indicates identity
Score > 17 indicates homology
WDVPQVQQSVKGQDGIQTFTR + Deamidated (NQ)
2830 593.6058 1777.7956 1777.7842 6.39 0 7 0.58 +1Score > 39 indicates identity
Score > 17 indicates homology
VQIWSCSGGANQQWR + 2 Deamidated (NQ)
3278 520.5431 2078.1433 2078.1160 13.1 1 7 0.29 +1Score > 34 indicates identity
Score > 14 indicates homology
VKYLGQLTSVPGYLNPSSR
3414 718.4139 2152.2199 2152.2442 -11.3 1 7 0.25 +1Score > 32 indicates identity
Score > 14 indicates homology
IIYVLLPNIMSISANHVKK
3804 806.4169 2416.2289 2416.1944 14.3 0 7 0.86 +1Score > 38 indicates identity
Score > 19 indicates homology
IVLLMGSNAQNDPSSPLNPYIR + 2 Deamidated (NQ); Oxidation (M)
4121 925.5098 2773.5076 2773.4659 15.0 1 7 0.36 +1Score > 33 indicates identity
Score > 15 indicates homology
RGNVMGIALGGLAMGVLVGPPFGSVLYE
2792 587.3586 1759.0540 1759.0243 16.8 1 7 0.31 +1Score > 26 indicates identity
Score > 15 indicates homology
LIFNSKITNIDLVLR + Deamidated (NQ)
3503 1102.0916 2202.1686 2202.1930 -11.0 1 7 0.59 +1Score > 37 indicates identity
Score > 17 indicates homology
VAGTAAGVTALQMDIKIQGITK + Deamidated (NQ); Oxidation (M)
3608 756.3901 2266.1485 2266.1668 -8.07 0 7 0.7 +1Score > 39 indicates identity
Score > 18 indicates homology
LFQIGVAPSSLTLVAVTHMHE + Deamidated (NQ); Oxidation (M)
3184 1007.4968 2012.9790 2012.9399 19.4 1 7 0.91 +1Score > 40 indicates identity
Score > 19 indicates homology
STSTDDITHIYTSSSSRR
3796 804.7495 2411.2267 2411.1964 12.6 1 7 0.37 +1Score > 38 indicates identity
Score > 15 indicates homology
EGVDLLGFLIITLNCNVTMSGK + 2 Deamidated (NQ); Oxidation (M)
3903 837.4705 2509.3897 2509.4011 -4.56 1 7 0.28 +1Score > 32 indicates identity
Score > 14 indicates homology
LAQLILSMPNLNLRGIMAVPSIK + 2 Deamidated (NQ); Oxidation (M)
3937 850.0952 2547.2638 2547.2664 -1.03 0 7 0.25 +1Score > 38 indicates identity
Score > 14 indicates homology
TSVSPLNVAQGASVNSSSQSVAQVTK + 2 Deamidated (NQ)
3500 733.4002 2197.1788 2197.2101 -14.3 0 7 0.46 +1Score > 36 indicates identity
Score > 16 indicates homology
GSMGLIINKPLPLMLNDIIK + 2 Deamidated (NQ); Oxidation (M)
2929 919.5040 1836.9934 1837.0282 -18.9 1 7 0.57 +1Score > 37 indicates identity
Score > 17 indicates homology
TARPGVVVGRGGAGIDSLR
242 506.2675 505.2602 505.2570 6.34 0 7 1 +1Score > 43 indicates identity
Score > 20 indicates homology
MGGLL + Oxidation (M)
2824 592.9856 1775.9350 1775.9128 12.5 0 7 0.83 +1Score > 39 indicates identity
Score > 19 indicates homology
DTAGGFQQLLLEPMLK + Oxidation (M)
3127 661.3167 1980.9283 1980.9218 3.29 1 7 0.34 +1Score > 39 indicates identity
Score > 15 indicates homology
ATKSFAQANGYVTDFFGR + 2 Deamidated (NQ)
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