MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3313 703.0201 2106.0385 2106.0224 7.61 1 8 0.45 +1Score > 39 indicates identity
Score > 17 indicates homology
MLGLDSMADAQELSIIGVVE + Oxidation (M)
1166 333.2074 996.6004 996.6080 -7.66 0 8 0.55 +1Score > 35 indicates identity
Score > 18 indicates homology
KPTKPAVTR
1666 609.3916 1216.7686 1216.7543 11.8 1 8 0.47 +1Score > 23 indicates identity
Score > 18 indicates homology
IALKFIINSAK
1952 673.8650 1345.7154 1345.7089 4.87 1 8 0.97 +1Score > 40 indicates identity
Score > 21 indicates homology
ANNLVSSVVSEVK + Deamidated (NQ)
2374 778.8537 1555.6928 1555.7075 -9.44 1 8 0.47 +1Score > 39 indicates identity
Score > 18 indicates homology
NAILNSDFQKQMK + 4 Deamidated (NQ); Oxidation (M)
2948 463.9782 1851.8837 1851.8753 4.53 0 8 0.35 +1Score > 40 indicates identity
Score > 16 indicates homology
SFQPMNINFGLFPPLE + 2 Deamidated (NQ)
2252 749.9404 1497.8662 1497.8402 17.4 1 8 0.78 +1Score > 35 indicates identity
Score > 20 indicates homology
ISIAGRLVADLLQE + Deamidated (NQ)
3139 994.9766 1987.9386 1987.9231 7.82 1 8 0.47 +1Score > 39 indicates identity
Score > 18 indicates homology
ITSDTEVFTMCTDTIVR
4066 902.4822 2704.4248 2704.3854 14.6 1 8 0.45 +1Score > 35 indicates identity
Score > 17 indicates homology
STKIYVGNVPIGGDAPIAVQSMTNTR + Oxidation (M)
1547 390.5664 1168.6774 1168.6816 -3.59 0 8 0.89 +1Score > 37 indicates identity
Score > 20 indicates homology
LASSGQIPGVIK
772 417.2206 832.4266 832.4403 -16.4 0 8 0.93 +1Score > 43 indicates identity
Score > 21 indicates homology
TNVSGSIR
2823 592.3230 1773.9472 1773.9665 -10.9 1 8 0.88 +1Score > 38 indicates identity
Score > 20 indicates homology
LNLVVQDFIFKHTAK + 2 Deamidated (NQ)
3512 735.3860 2203.1362 2203.1334 1.28 1 8 0.34 +1Score > 38 indicates identity
Score > 16 indicates homology
IIVSELNVLNTAMTPPFTIE + 2 Deamidated (NQ)
3638 1147.5852 2293.1558 2293.1240 13.9 1 8 0.56 +1Score > 38 indicates identity
Score > 18 indicates homology
FWLGNDHIHALTAQGTNELR + Deamidated (NQ)
2082 705.3968 1408.7790 1408.7561 16.3 1 8 0.64 +1Score > 37 indicates identity
Score > 19 indicates homology
NELLLLDLSHNK + Deamidated (NQ)
3470 547.7710 2187.0549 2187.0365 8.40 1 8 0.55 +1Score > 39 indicates identity
Score > 18 indicates homology
GDVMDKTNGLPVQNGIDADVK + 2 Deamidated (NQ)
2559 408.7244 1630.8685 1630.8678 0.41 0 8 0.51 +1Score > 39 indicates identity
Score > 18 indicates homology
INDGVAIALYGLNAAR + Deamidated (NQ)
2953 928.4595 1854.9044 1854.8748 16.0 0 8 0.96 +1Score > 40 indicates identity
Score > 21 indicates homology
EPSGGNNWAPLQAGDTLK + Deamidated (NQ)
3738 786.7617 2357.2633 2357.2267 15.5 1 8 0.21 +1Score > 36 indicates identity
Score > 14 indicates homology
NLYIIYSDFTVNKNIQLAAR + 2 Deamidated (NQ)
3284 696.0280 2085.0622 2085.0677 -2.65 1 8 0.64 +1Score > 39 indicates identity
Score > 19 indicates homology
GLTLYGNVDIRTLNMSFR + Oxidation (M)
3564 747.0310 2238.0712 2238.0429 12.6 0 8 0.61 +1Score > 39 indicates identity
Score > 19 indicates homology
IWSVYCHYYHVLQNNSR
3618 758.7445 2273.2117 2273.1837 12.3 1 8 0.46 +1Score > 36 indicates identity
Score > 17 indicates homology
KSNFSLAILNVGAPAAGMNAAVR + 2 Deamidated (NQ)
1162 498.7815 995.5484 995.5400 8.49 0 8 0.73 +1Score > 38 indicates identity
Score > 19 indicates homology
APPTTAVPSR
1937 668.8314 1335.6482 1335.6592 -8.18 1 8 0.92 +1Score > 41 indicates identity
Score > 20 indicates homology
VVTESIGQVMQK + 2 Deamidated (NQ); Oxidation (M)
3482 731.3945 2191.1617 2191.1637 -0.92 1 8 0.38 +1Score > 37 indicates identity
Score > 17 indicates homology
QQFGRIINIASVNGLVGFAGK + 3 Deamidated (NQ)
932 906.4987 905.4914 905.4971 -6.22 0 8 0.92 +1Score > 41 indicates identity
Score > 20 indicates homology
VHQIPSPK + Deamidated (NQ)
1288 351.2122 1050.6148 1050.6046 9.64 1 8 0.58 +1Score > 33 indicates identity
Score > 18 indicates homology
TVLAARQHR
3538 1112.0242 2222.0338 2222.0703 -16.4 1 8 0.87 +1Score > 39 indicates identity
Score > 20 indicates homology
TEYGLNVVSGDITDITTPDGR
3832 813.7896 2438.3470 2438.3429 1.67 1 8 0.76 +1Score > 32 indicates identity
Score > 20 indicates homology
LQIMAHISLLVGAGVMSLLAKWA + Deamidated (NQ); Oxidation (M)
2560 816.4534 1630.8922 1630.9116 -11.9 1 8 0.92 +1Score > 38 indicates identity
Score > 20 indicates homology
LFGLPMAVKDNLSVK
3641 1147.5865 2293.1584 2293.1240 15.0 1 8 0.94 +1Score > 38 indicates identity
Score > 21 indicates homology
FWLGNDHIHALTAQGTNELR + Deamidated (NQ)
684 392.7512 783.4878 783.4854 3.09 0 8 0.65 +1Score > 35 indicates identity
Score > 19 indicates homology
VIQSLPK
2672 563.2996 1686.8770 1686.9039 -16.0 0 8 0.36 +1Score > 40 indicates identity
Score > 16 indicates homology
TTLNIQAIQQDSLIK + 2 Deamidated (NQ)
4108 918.8070 2753.3992 2753.4132 -5.09 1 8 0.19 +1Score > 37 indicates identity
Score > 14 indicates homology
NPLAAVMMGLIYVNPEGVDGKPDPLK + Oxidation (M)
3568 748.4068 2242.1986 2242.1633 15.7 1 8 0.28 +1Score > 36 indicates identity
Score > 15 indicates homology
YVGNKINAGLYIFNSAILDR + 2 Deamidated (NQ)
1465 378.8714 1133.5924 1133.5863 5.38 1 8 0.57 +1Score > 42 indicates identity
Score > 18 indicates homology
NMLLTGGRQK + Deamidated (NQ); Oxidation (M)
3116 658.0286 1971.0640 1971.1000 -18.3 1 8 0.81 +1Score > 37 indicates identity
Score > 20 indicates homology
SLIQKTTNGVTAALGALNAK + Deamidated (NQ)
2248 749.9377 1497.8608 1497.8555 3.58 1 8 0.82 +1Score > 35 indicates identity
Score > 20 indicates homology
AQLAAAEVPFLLVR + Deamidated (NQ)
3137 663.3510 1987.0312 1987.0271 2.06 1 8 0.21 +1Score > 39 indicates identity
Score > 14 indicates homology
FSMPKQVGIDLVQMHLK + Deamidated (NQ); Oxidation (M)
3993 874.4418 2620.3036 2620.2798 9.08 1 8 0.23 +1Score > 38 indicates identity
Score > 14 indicates homology
VVDASIMPSMVSGNLNAPTIMIAEK + Deamidated (NQ); 2 Oxidation (M)
3691 772.4233 2314.2481 2314.2209 11.7 0 8 1 +1Score > 35 indicates identity
Score > 21 indicates homology
IGASIPIVSNGSGLFSSFSIFSK
3309 1050.5541 2099.0936 2099.0867 3.31 1 8 0.98 +1Score > 38 indicates identity
Score > 21 indicates homology
MQAQASIEGGPQLSMLGLLR
3377 1068.6093 2135.2040 2135.1739 14.1 1 8 0.62 +1Score > 32 indicates identity
Score > 19 indicates homology
WLPSLNPLDINVVVTGKDR
3169 1005.5563 2009.0980 2009.0582 19.8 1 8 0.24 +1Score > 36 indicates identity
Score > 15 indicates homology
QKQTLLNIGFGNFVVSSR + 2 Deamidated (NQ)
3702 1162.5925 2323.1704 2323.1743 -1.65 1 8 0.75 +1Score > 38 indicates identity
Score > 19 indicates homology
MQTAALAAGTDALPKLDWASHR
2480 535.6456 1603.9150 1603.9450 -18.7 0 8 0.83 +1Score > 34 indicates identity
Score > 20 indicates homology
ILGQFTKPFALTIR
828 856.4462 855.4389 855.4450 -7.13 1 8 0.8 +1Score > 38 indicates identity
Score > 20 indicates homology
QDVELPR
3223 1019.5565 2037.0984 2037.0629 17.4 1 8 0.95 +1Score > 36 indicates identity
Score > 20 indicates homology
KTQQIAQQLILGSSSIYR + 4 Deamidated (NQ)
874 439.2634 876.5122 876.5102 2.29 1 8 0.82 +1Score > 38 indicates identity
Score > 20 indicates homology
ASMISKLK
1825 427.5989 1279.7749 1279.7864 -8.98 0 8 0.73 +1Score > 33 indicates identity
Score > 19 indicates homology
GLSLLVPVQLNK
2004 458.8862 1373.6368 1373.6423 -3.99 0 8 0.72 +1Score > 40 indicates identity
Score > 19 indicates homology
TAATNLAQAQAQGE + Deamidated (NQ)
3135 663.3465 1987.0177 1987.0098 3.98 1 8 0.61 +1Score > 39 indicates identity
Score > 19 indicates homology
LAMLSTHYRQPINWTR + Deamidated (NQ)
2993 627.6279 1879.8619 1879.8655 -1.94 0 8 0.98 +1Score > 39 indicates identity
Score > 21 indicates homology
LQAIIVNSGNANACTGMK + 3 Deamidated (NQ); Oxidation (M)
3028 477.5091 1906.0073 1905.9982 4.77 1 8 0.94 +1Score > 38 indicates identity
Score > 20 indicates homology
QMNKINLQNVFAVTLR + 2 Deamidated (NQ); Oxidation (M)
3119 658.3365 1971.9877 1971.9974 -4.92 1 8 0.26 +1Score > 39 indicates identity
Score > 15 indicates homology
SIQVNKTQTLGDQQAQGR + Deamidated (NQ)
400 322.1881 642.3616 642.3561 8.57 1 8 0.6 +1Score > 35 indicates identity
Score > 18 indicates homology
NRTPR
3238 1023.5369 2045.0592 2045.0840 -12.1 1 8 0.8 +1Score > 38 indicates identity
Score > 20 indicates homology
GVFSMQQRQALAQISLPR + Oxidation (M)
3361 710.3771 2128.1095 2128.0834 12.2 1 8 0.9 +1Score > 38 indicates identity
Score > 20 indicates homology
IGTVQQCLVDAIEGDIAVAR + Deamidated (NQ)
2381 520.6223 1558.8451 1558.8606 -9.98 1 8 0.6 +1Score > 38 indicates identity
Score > 18 indicates homology
QQKYAILGVPSLLE + Deamidated (NQ)
2906 909.9445 1817.8744 1817.8982 -13.0 1 8 0.65 +1Score > 40 indicates identity
Score > 19 indicates homology
HLLDSLSVMSFIENGR + Deamidated (NQ)
3391 716.0552 2145.1438 2145.1099 15.8 0 8 0.73 +1Score > 37 indicates identity
Score > 19 indicates homology
INLDNLGLLTMQANITGTSR + Deamidated (NQ)
3216 1017.5451 2033.0756 2033.0689 3.31 1 8 0.75 +1Score > 38 indicates identity
Score > 19 indicates homology
EMLQGLLLWLLLSMGGAR + Deamidated (NQ); 2 Oxidation (M)
2773 870.4803 1738.9460 1738.9465 -0.26 0 8 0.39 +1Score > 37 indicates identity
Score > 17 indicates homology
LIGSVDDLLPSLPASSR
3266 692.7170 2075.1292 2075.0899 18.9 1 8 0.19 +1Score > 36 indicates identity
Score > 13 indicates homology
ATDLDVFLKGGAALDINSVR + Deamidated (NQ)
3443 1087.5537 2173.0928 2173.0611 14.6 1 8 0.41 +1Score > 39 indicates identity
Score > 17 indicates homology
QNTRPVSEVNASLDLNTASR + 2 Deamidated (NQ)
2761 578.2634 1731.7684 1731.7799 -6.66 1 8 0.41 +1Score > 39 indicates identity
Score > 17 indicates homology
ADTTTGSAAGPPLSEDDK
3594 564.0352 2252.1117 2252.1319 -8.99 0 8 0.31 +1Score > 39 indicates identity
Score > 16 indicates homology
IALMQAQLSAILDYIAMLQE + 2 Deamidated (NQ); Oxidation (M)
3221 1019.0269 2036.0392 2036.0367 1.23 1 8 0.99 +1Score > 39 indicates identity
Score > 21 indicates homology
LHFFTPSSIGAFGDSTPKK
3526 1106.5432 2211.0718 2211.0596 5.53 1 8 0.43 +1Score > 39 indicates identity
Score > 17 indicates homology
EITGLWQGSLFNANYDVQR + Deamidated (NQ)
1987 456.2315 1365.6727 1365.6637 6.59 1 8 0.91 +1Score > 41 indicates identity
Score > 20 indicates homology
RSHDALVASNPAE
3051 641.6551 1921.9435 1921.9765 -17.2 1 8 0.9 +1Score > 40 indicates identity
Score > 20 indicates homology
QRPTGRPTSARQAMGHR + Oxidation (M)
3675 1154.0580 2306.1014 2306.1147 -5.77 1 8 0.49 +1Score > 39 indicates identity
Score > 17 indicates homology
HQPDIVICVGQAGGRMQITPE + Deamidated (NQ)
1040 947.5008 946.4935 946.4872 6.65 0 8 0.32 +1Score > 42 indicates identity
Score > 16 indicates homology
GAVSNPAFGK
1808 320.1845 1276.7089 1276.6887 15.8 1 8 0.4 +1Score > 37 indicates identity
Score > 17 indicates homology
NLIRASYNVAR + Deamidated (NQ)
2880 602.3285 1803.9637 1803.9441 10.9 1 8 0.82 +1Score > 38 indicates identity
Score > 20 indicates homology
TLVYMADEQPVIAVVR + Deamidated (NQ)
3504 735.0666 2202.1780 2202.1354 19.3 1 8 0.94 +1Score > 36 indicates identity
Score > 20 indicates homology
LFDMVETLSHNNNVSILLK + Oxidation (M)
3620 759.3496 2275.0270 2275.0024 10.8 1 8 0.44 +1Score > 37 indicates identity
Score > 17 indicates homology
LILSNQYYLMSQMDPENAE + Deamidated (NQ); Oxidation (M)
3516 736.3893 2206.1461 2206.1706 -11.1 1 8 0.18 +1Score > 38 indicates identity
Score > 13 indicates homology
GLTPSQIGVILRDSHGVAQTR + 2 Deamidated (NQ)
2744 576.0106 1725.0100 1724.9785 18.3 1 8 0.63 +1Score > 31 indicates identity
Score > 18 indicates homology
VGLKLDQAGLDVLGTAR
2931 613.3408 1837.0006 1836.9767 13.0 1 8 0.94 +1Score > 36 indicates identity
Score > 20 indicates homology
AGLGLAQEAPVVALMPGSR + Deamidated (NQ)
3121 990.5240 1979.0334 1979.0146 9.54 1 8 0.81 +1Score > 38 indicates identity
Score > 20 indicates homology
YDTLAQMLTLGNIRAGNK + Deamidated (NQ)
3424 722.3622 2164.0648 2164.0432 9.99 1 8 0.73 +1Score > 39 indicates identity
Score > 19 indicates homology
ASLENPLAAVMMGLIYVNPE + Deamidated (NQ); 2 Oxidation (M)
1358 544.2924 1086.5702 1086.5710 -0.68 0 8 0.61 +1Score > 41 indicates identity
Score > 18 indicates homology
GVPDTPGVAFK
3695 579.7919 2315.1385 2315.1355 1.29 1 8 0.85 +1Score > 39 indicates identity
Score > 20 indicates homology
WVDPQLSSEALVQQITMAGLE + Deamidated (NQ)
4395 1055.5162 3163.5268 3163.5282 -0.46 1 8 0.32 +1Score > 36 indicates identity
Score > 15 indicates homology
LAATFQSDIPSKQNQAQNGNQSHLPPQSR + 2 Deamidated (NQ)
1612 597.8237 1193.6328 1193.6478 -12.5 1 8 0.91 +1Score > 40 indicates identity
Score > 20 indicates homology
ILLQEYIMR + Oxidation (M)
2676 846.8771 1691.7396 1691.7355 2.44 0 8 0.9 +1Score > 38 indicates identity
Score > 20 indicates homology
NGANAGMIVGSCQNAAR + 2 Deamidated (NQ)
2766 867.4825 1732.9504 1732.9247 14.9 0 8 0.52 +1Score > 37 indicates identity
Score > 18 indicates homology
ITTVQLAIDYINANLA + Deamidated (NQ)
3597 752.3781 2254.1125 2254.0827 13.2 1 8 0.69 +1Score > 39 indicates identity
Score > 19 indicates homology
DGIQVDMIFYKGNAISVSAPE + Deamidated (NQ)
2654 837.4692 1672.9238 1672.9413 -10.4 0 8 0.55 +1Score > 36 indicates identity
Score > 18 indicates homology
LAGAHHPVHLLPLGIE
2731 574.3109 1719.9109 1719.9083 1.49 1 8 0.47 +1Score > 39 indicates identity
Score > 17 indicates homology
QKLFQADNGLPVFLK + 3 Deamidated (NQ)
3399 537.7668 2147.0381 2147.0092 13.4 1 8 0.27 +1Score > 40 indicates identity
Score > 15 indicates homology
DQNVQAILAGAPELMDFLGE + Deamidated (NQ); Oxidation (M)
2949 927.9570 1853.8994 1853.9231 -12.8 0 8 0.54 +1Score > 40 indicates identity
Score > 18 indicates homology
TIDNTGKPTNVQANPQR + Deamidated (NQ)
3478 1096.5350 2191.0554 2191.0613 -2.65 1 8 0.34 +1Score > 39 indicates identity
Score > 16 indicates homology
MSGLDMIQKLNDIGAANGIGR + 2 Deamidated (NQ); Oxidation (M)
4220 961.8224 2882.4454 2882.4082 12.9 0 8 0.27 +1Score > 37 indicates identity
Score > 15 indicates homology
SLNVTLNLSMDNSFVIDTPLMWLNK + 2 Deamidated (NQ); Oxidation (M)
2963 930.4800 1858.9454 1858.9173 15.1 1 8 0.78 +1Score > 40 indicates identity
Score > 19 indicates homology
INRDGDVSDIAVPFANR + Deamidated (NQ)
3075 646.3497 1936.0273 1936.0492 -11.3 1 8 0.75 +1Score > 38 indicates identity
Score > 19 indicates homology
VGKLLPFMIDLSAFVNR + Deamidated (NQ); Oxidation (M)
3113 657.9999 1970.9779 1970.9949 -8.64 0 8 0.54 +1Score > 40 indicates identity
Score > 18 indicates homology
TSDHFIADLAVGLNAGQIK + 2 Deamidated (NQ)
876 440.2522 878.4898 878.4861 4.23 1 8 0.55 +1Score > 38 indicates identity
Score > 18 indicates homology
KAAVQYAK + Deamidated (NQ)
2779 582.0031 1742.9875 1742.9607 15.4 0 8 0.64 +1Score > 34 indicates identity
Score > 18 indicates homology
LVGVLNWAAQIYPGIK + 2 Deamidated (NQ)
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