MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2274 755.3892 1508.7638 1508.7731 -6.13 1 9 0.28 +1Score > 40 indicates identity
Score > 16 indicates homology
GEGAFKPLMGLVMK + 2 Oxidation (M)
2468 533.2822 1596.8248 1596.7995 15.8 0 9 0.36 +1Score > 40 indicates identity
Score > 17 indicates homology
TNAGPLQGTIQVPGDK + 2 Deamidated (NQ)
2821 887.4681 1772.9216 1772.8866 19.8 0 9 0.52 +1Score > 40 indicates identity
Score > 19 indicates homology
LNTILNTMSTIYSTGK + Deamidated (NQ); Oxidation (M)
1795 637.3329 1272.6512 1272.6449 5.00 1 9 0.93 +1Score > 41 indicates identity
Score > 21 indicates homology
QLVALEQLQTE + 2 Deamidated (NQ)
1927 665.3443 1328.6740 1328.6646 7.13 0 9 0.71 +1Score > 41 indicates identity
Score > 20 indicates homology
LNVVNTPQMIAE + Deamidated (NQ)
3091 651.3326 1950.9760 1950.9660 5.11 1 9 0.99 +1Score > 40 indicates identity
Score > 22 indicates homology
SNRHIYAQVIDDVHQR + Deamidated (NQ)
3374 712.7391 2135.1955 2135.1991 -1.68 0 9 0.8 +1Score > 32 indicates identity
Score > 21 indicates homology
LPLTTVSQLFVQLIIGGHAE
3901 837.4662 2509.3768 2509.3462 12.2 1 9 0.28 +1Score > 33 indicates identity
Score > 16 indicates homology
VAEMLAPVLTGNDLILVQGAGNIGK + Deamidated (NQ); Oxidation (M)
1447 565.8241 1129.6336 1129.6343 -0.54 1 9 0.95 +1Score > 40 indicates identity
Score > 21 indicates homology
LTAKQSLLQE
2850 598.3006 1791.8800 1791.8686 6.35 1 9 0.83 +1Score > 41 indicates identity
Score > 21 indicates homology
MNWAISNLTGGNRVSR + Deamidated (NQ); Oxidation (M)
2969 622.3367 1863.9883 1864.0135 -13.5 1 9 0.79 +1Score > 38 indicates identity
Score > 21 indicates homology
LFVNFDPQILILFRE + Deamidated (NQ)
3144 996.0228 1990.0310 1990.0483 -8.68 1 9 0.87 +1Score > 39 indicates identity
Score > 21 indicates homology
LAGNSIIIFARTVNDTQR + 2 Deamidated (NQ)
3975 862.1168 2583.3286 2583.3553 -10.3 1 9 0.37 +1Score > 37 indicates identity
Score > 17 indicates homology
VDPVNFKLLSQCIQVVLAAHMGK + Deamidated (NQ); Oxidation (M)
3627 760.4053 2278.1941 2278.1488 19.9 1 9 0.82 +1Score > 37 indicates identity
Score > 21 indicates homology
QHADLQRQSADALLAMLPQR + Deamidated (NQ); Oxidation (M)
2180 366.4666 1461.8373 1461.8113 17.8 1 9 0.64 +1Score > 34 indicates identity
Score > 20 indicates homology
MTVLTQEAIITVK + Oxidation (M)
2479 802.4159 1602.8172 1602.8287 -7.14 1 9 0.39 +1Score > 40 indicates identity
Score > 18 indicates homology
AIDRSGVVLMVINAE + Deamidated (NQ); Oxidation (M)
3293 1045.5602 2089.1058 2089.1419 -17.3 1 9 0.95 +1Score > 38 indicates identity
Score > 21 indicates homology
LNVLGEGVDIVIRPPDAALE
4416 1061.5283 3181.5631 3181.5682 -1.61 1 9 0.17 +1Score > 36 indicates identity
Score > 14 indicates homology
LAMHGTHNMLNSLAAIGVATQMGIADDKIR + Deamidated (NQ); 2 Oxidation (M)
1265 346.8706 1037.5900 1037.5943 -4.20 1 9 0.39 +1Score > 35 indicates identity
Score > 18 indicates homology
KLFVGMISK + Oxidation (M)
1984 683.3270 1364.6394 1364.6554 -11.7 0 9 0.66 +1Score > 41 indicates identity
Score > 20 indicates homology
YWLYPYGTFR
2264 752.3927 1502.7708 1502.7538 11.3 1 9 0.42 +1Score > 41 indicates identity
Score > 18 indicates homology
LTVGVAMSEQLLPE + Deamidated (NQ); Oxidation (M)
3189 1010.0085 2018.0024 2018.0216 -9.51 1 9 0.92 +1Score > 40 indicates identity
Score > 21 indicates homology
LESIVMNWLGQMLNLPK + Deamidated (NQ); 2 Oxidation (M)
1462 378.8654 1133.5744 1133.5717 2.38 1 9 0.83 +1Score > 42 indicates identity
Score > 21 indicates homology
QVTLEPYER
2178 487.9282 1460.7628 1460.7736 -7.39 1 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
NLSPSPQHPVTKR + Deamidated (NQ)
2668 843.4811 1684.9476 1684.9247 13.6 1 9 0.62 +1Score > 36 indicates identity
Score > 20 indicates homology
QAQILLESVNIITNK + 2 Deamidated (NQ)
3717 585.0399 2336.1305 2336.1179 5.39 1 9 0.41 +1Score > 39 indicates identity
Score > 18 indicates homology
NASKGPHSPQVPSQVQSPMTSR + Deamidated (NQ); Oxidation (M)
2895 906.9430 1811.8714 1811.8876 -8.94 0 9 0.59 +1Score > 40 indicates identity
Score > 19 indicates homology
GGAPNVAVVMVDGWPTDK
3234 680.7229 2039.1469 2039.1150 15.6 1 9 0.85 +1Score > 33 indicates identity
Score > 21 indicates homology
IGNQLRATLLLQDINLLE + 3 Deamidated (NQ)
4097 687.6025 2746.3809 2746.3815 -0.24 0 9 0.86 +1Score > 37 indicates identity
Score > 21 indicates homology
QLGNQLMMAINLQINQQQLMSVSK + Deamidated (NQ); Oxidation (M)
3164 1004.5883 2007.1620 2007.1405 10.8 1 9 0.27 +1Score > 29 indicates identity
Score > 16 indicates homology
IVLGIDPALGSLGWAVVAKE
2658 558.6526 1672.9360 1672.9359 0.026 1 9 0.74 +1Score > 36 indicates identity
Score > 20 indicates homology
LVQSQLLKSVVSGSAR + 2 Deamidated (NQ)
1464 378.8713 1133.5921 1133.5829 8.09 1 9 0.78 +1Score > 42 indicates identity
Score > 20 indicates homology
GAGNFLAIRSE
3552 745.4353 2233.2841 2233.2583 11.5 1 9 0.84 +1Score > 29 indicates identity
Score > 21 indicates homology
SLTRFGFTLIIGGAIGNLIDR
2188 735.3715 1468.7284 1468.7449 -11.2 0 9 0.73 +1Score > 40 indicates identity
Score > 20 indicates homology
SQLIDILDFDYK
2531 808.4574 1614.9002 1614.8689 19.4 1 9 0.58 +1Score > 37 indicates identity
Score > 19 indicates homology
TGGGTQGDSNVLKIIR
2771 578.9674 1733.8804 1733.8757 2.71 0 9 0.46 +1Score > 40 indicates identity
Score > 18 indicates homology
ILQNMADTISLIAGQK + 3 Deamidated (NQ); Oxidation (M)
446 671.3842 670.3769 670.3650 17.8 0 9 0.46 +1Score > 37 indicates identity
Score > 18 indicates homology
AQVPAGK + Deamidated (NQ)
3423 721.7231 2162.1475 2162.1556 -3.74 1 9 0.95 +1Score > 37 indicates identity
Score > 21 indicates homology
SLLATHNTNAAGRAIAQLPSR + Deamidated (NQ)
1800 425.5518 1273.6336 1273.6085 19.7 0 9 0.78 +1Score > 41 indicates identity
Score > 20 indicates homology
TGATTIAHGCTGK
2828 593.3222 1776.9448 1776.9509 -3.45 0 9 0.67 +1Score > 39 indicates identity
Score > 20 indicates homology
AISLAYATGQSLLGLIGE + Deamidated (NQ)
1286 1050.5592 1049.5519 1049.5539 -1.89 0 9 0.79 +1Score > 40 indicates identity
Score > 20 indicates homology
ALSAMSLSVR + Oxidation (M)
3161 668.0254 2001.0544 2001.0425 5.92 1 9 0.35 +1Score > 39 indicates identity
Score > 17 indicates homology
DRSGASNSLTLDRPLMLR
1559 391.5690 1171.6852 1171.6673 15.2 1 9 0.91 +1Score > 37 indicates identity
Score > 21 indicates homology
ANTGGLSKLVGR
2337 769.8981 1537.7816 1537.7698 7.72 0 9 0.53 +1Score > 40 indicates identity
Score > 19 indicates homology
YISDMLNTVLKPE + Oxidation (M)
2535 809.9174 1617.8202 1617.7893 19.1 1 9 0.89 +1Score > 41 indicates identity
Score > 21 indicates homology
LAGAAQSTADKQAACR
2746 576.6263 1726.8571 1726.8308 15.2 1 9 0.77 +1Score > 40 indicates identity
Score > 20 indicates homology
SGHNVAEVMNLIGDVR + Deamidated (NQ); Oxidation (M)
2303 759.9179 1517.8212 1517.8487 -18.1 1 9 0.66 +1Score > 39 indicates identity
Score > 20 indicates homology
ILNKQSGMLGISLK + Deamidated (NQ); Oxidation (M)
3296 1046.5596 2091.1046 2091.0888 7.57 1 9 0.54 +1Score > 37 indicates identity
Score > 19 indicates homology
IPFDILINGQFLRTTVDE + Deamidated (NQ)
1383 368.5266 1102.5580 1102.5441 12.6 0 9 0.73 +1Score > 42 indicates identity
Score > 20 indicates homology
AQLVVCQQR + 2 Deamidated (NQ)
440 335.7115 669.4084 669.4173 -13.3 1 9 0.77 +1Score > 34 indicates identity
Score > 20 indicates homology
GAPKAVK
3407 1076.5602 2151.1058 2151.0994 2.99 1 9 0.21 +1Score > 38 indicates identity
Score > 15 indicates homology
AVDFRTLLTLCAQSVSAGDK
2410 524.9780 1571.9122 1571.9148 -1.65 1 9 0.5 +1Score > 33 indicates identity
Score > 18 indicates homology
VVRVQPPAPPVAVSR + Deamidated (NQ)
3409 718.0455 2151.1147 2151.1245 -4.58 1 9 0.73 +1Score > 38 indicates identity
Score > 20 indicates homology
RNMLSYDQLLDSSLLVGVK + Deamidated (NQ)
3497 733.3656 2197.0750 2197.0434 14.4 0 9 0.99 +1Score > 39 indicates identity
Score > 21 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
3359 710.0457 2127.1153 2127.0960 9.05 1 9 0.59 +1Score > 38 indicates identity
Score > 19 indicates homology
AADTAGDLAGLEIAGPGFINVR
2324 765.9388 1529.8630 1529.8929 -19.5 0 9 0.47 +1Score > 36 indicates identity
Score > 18 indicates homology
LQSLALYVVAAINR
3609 756.3937 2266.1593 2266.1490 4.53 1 9 0.6 +1Score > 38 indicates identity
Score > 19 indicates homology
VDGAVIEIPLMAMNFTGGIFR + Oxidation (M)
2045 464.2625 1389.7657 1389.7616 2.93 1 9 0.67 +1Score > 38 indicates identity
Score > 20 indicates homology
GEGIAGLATALVYR
2663 842.4498 1682.8850 1682.8814 2.16 1 9 0.46 +1Score > 39 indicates identity
Score > 18 indicates homology
IDICAIGSKAVSFFR
3744 787.7613 2360.2621 2360.2410 8.94 1 9 1 +1Score > 36 indicates identity
Score > 21 indicates homology
IADVGLAKLVTDVAPDNVTMYR
3349 707.7039 2120.0899 2120.0870 1.34 1 9 0.61 +1Score > 38 indicates identity
Score > 19 indicates homology
RGALQAIDQMSLFRPLCK + Deamidated (NQ); Oxidation (M)
2727 860.4235 1718.8324 1718.8152 10.1 1 9 0.49 +1Score > 40 indicates identity
Score > 18 indicates homology
EQVSIFPATHFVTNE + Deamidated (NQ)
3147 498.5261 1990.0753 1990.0591 8.13 0 9 0.77 +1Score > 37 indicates identity
Score > 20 indicates homology
ADVVLIGAGIMSSTLGAMLR + Oxidation (M)
2111 711.8326 1421.6506 1421.6537 -2.12 1 9 0.8 +1Score > 40 indicates identity
Score > 20 indicates homology
YKYLPNMNSFK + 2 Deamidated (NQ); Oxidation (M)
3520 1105.1345 2208.2544 2208.2531 0.61 1 9 0.84 +1Score > 31 indicates identity
Score > 21 indicates homology
NIINLEPVLNVLRWHLHK + Deamidated (NQ)
1804 426.5642 1276.6708 1276.6775 -5.30 1 9 0.61 +1Score > 41 indicates identity
Score > 19 indicates homology
FLQNSDGKLVR + Deamidated (NQ)
1285 1048.5516 1047.5443 1047.5349 9.00 1 9 0.75 +1Score > 42 indicates identity
Score > 20 indicates homology
QDFVRALAE
2743 575.9982 1724.9728 1724.9560 9.73 1 9 0.66 +1Score > 35 indicates identity
Score > 19 indicates homology
SNVILIVIDAEQGIIE
3342 530.2810 2117.0949 2117.0609 16.1 1 9 0.79 +1Score > 39 indicates identity
Score > 20 indicates homology
VVICTPGRMIDLLTANNGR + 2 Deamidated (NQ); Oxidation (M)
3430 722.7241 2165.1505 2165.1328 8.16 1 9 0.32 +1Score > 37 indicates identity
Score > 16 indicates homology
VTINPAIAHGISSQVGSVETGK + Deamidated (NQ)
3038 638.6935 1913.0587 1913.0218 19.3 1 9 0.72 +1Score > 35 indicates identity
Score > 20 indicates homology
TLTANVTSLALGPVQEGSR
3242 513.2548 2048.9901 2049.0102 -9.82 1 9 0.24 +1Score > 40 indicates identity
Score > 15 indicates homology
AKLPGRPTSDCFFSDPVR
1225 340.8636 1019.5690 1019.5552 13.5 0 9 0.76 +1Score > 39 indicates identity
Score > 20 indicates homology
FLLNNPFR
2927 918.4647 1834.9148 1834.9465 -17.2 0 9 0.6 +1Score > 40 indicates identity
Score > 19 indicates homology
TLASIINDNIFNQFPK + Deamidated (NQ)
3259 1031.0336 2060.0526 2060.0704 -8.63 1 9 0.41 +1Score > 39 indicates identity
Score > 17 indicates homology
HGWPPAGGSALFQRLVDPR
653 384.7074 767.4002 767.4038 -4.66 0 9 0.78 +1Score > 37 indicates identity
Score > 20 indicates homology
IASHGQR
2721 857.4451 1712.8756 1712.9057 -17.5 1 9 0.86 +1Score > 40 indicates identity
Score > 20 indicates homology
DLADKLAQTNAAVLNR + Deamidated (NQ)
3065 644.3655 1930.0747 1930.0524 11.6 0 9 0.54 +1Score > 34 indicates identity
Score > 18 indicates homology
QSALVQVFNKPVQTITR + 2 Deamidated (NQ)
3514 552.2950 2205.1509 2205.1187 14.6 1 9 0.95 +1Score > 37 indicates identity
Score > 21 indicates homology
IDGVEPMLHPLHMFQRLR + Deamidated (NQ); Oxidation (M)
2393 783.9011 1565.7876 1565.7582 18.8 1 9 0.89 +1Score > 41 indicates identity
Score > 21 indicates homology
MVSQAMQWLALEK + 2 Oxidation (M)
2550 543.3274 1626.9604 1626.9444 9.85 1 9 0.44 +1Score > 30 indicates identity
Score > 18 indicates homology
DGVLLINLLEIISSK + Deamidated (NQ)
1404 557.2660 1112.5174 1112.5324 -13.5 1 9 0.49 +1Score > 40 indicates identity
Score > 18 indicates homology
KWVQNYMK + Deamidated (NQ); Oxidation (M)
1735 413.8957 1238.6653 1238.6693 -3.22 0 9 0.91 +1Score > 39 indicates identity
Score > 21 indicates homology
LNSLMVPHLSK + Deamidated (NQ)
1356 362.8672 1085.5798 1085.5869 -6.58 0 9 0.48 +1Score > 41 indicates identity
Score > 18 indicates homology
QHALLYNVK + Deamidated (NQ)
1674 610.8359 1219.6572 1219.6561 0.97 0 9 0.45 +1Score > 40 indicates identity
Score > 18 indicates homology
ISLGLFSNVNR + Deamidated (NQ)
1366 546.8095 1091.6044 1091.6226 -16.7 0 9 0.61 +1Score > 39 indicates identity
Score > 19 indicates homology
LGNILVAYTK + Deamidated (NQ)
3442 725.3682 2173.0828 2173.1167 -15.6 1 9 0.86 +1Score > 39 indicates identity
Score > 20 indicates homology
QQQLPQVAWLAEHLAAQLE + Deamidated (NQ)
1881 437.2238 1308.6496 1308.6687 -14.6 0 9 0.93 +1Score > 41 indicates identity
Score > 21 indicates homology
AGASAWNGHLAVR
2558 816.4163 1630.8180 1630.8025 9.54 1 9 0.6 +1Score > 41 indicates identity
Score > 19 indicates homology
CAFRVLLPSDPVQE + Deamidated (NQ)
1785 635.3603 1268.7060 1268.7088 -2.19 0 9 0.35 +1Score > 38 indicates identity
Score > 17 indicates homology
DAGQIIGLNVLR + Deamidated (NQ)
2091 706.4281 1410.8416 1410.8558 -10.1 1 9 0.78 +1Score > 33 indicates identity
Score > 20 indicates homology
GILSTPAVSAVIRK
1578 393.8892 1178.6458 1178.6659 -17.1 1 9 0.98 +1Score > 38 indicates identity
Score > 21 indicates homology
KVTVFDTTLR
2397 783.9326 1565.8506 1565.8487 1.25 0 8 0.49 +1Score > 38 indicates identity
Score > 18 indicates homology
LGNLLAQLMGLPTPE
2350 515.2885 1542.8437 1542.8617 -11.7 1 8 0.88 +1Score > 38 indicates identity
Score > 20 indicates homology
SILSDLEGTAIVAVR
3425 722.4137 2164.2193 2164.1813 17.5 1 8 0.49 +1Score > 32 indicates identity
Score > 18 indicates homology
VVQMLVDYITKNSIAGVGLK + Deamidated (NQ); Oxidation (M)
4101 917.4517 2749.3333 2749.3307 0.95 0 8 0.59 +1Score > 38 indicates identity
Score > 19 indicates homology
AHADASVSAQALQLDHNGINFALNIR + 4 Deamidated (NQ)
2270 753.9338 1505.8530 1505.8752 -14.7 0 8 0.42 +1Score > 35 indicates identity
Score > 17 indicates homology
LLDLLHLQLICR
3529 739.3673 2215.0801 2215.1120 -14.4 1 8 0.32 +1Score > 39 indicates identity
Score > 16 indicates homology
LALADNVPYNVVDSQNLKNE
2119 475.5775 1423.7107 1423.7195 -6.18 0 8 0.69 +1Score > 41 indicates identity
Score > 19 indicates homology
YATADLSVQTDIK
3183 503.7774 2011.0805 2011.0594 10.5 0 8 0.6 +1Score > 37 indicates identity
Score > 19 indicates homology
ASVMLHLPALVMTQQISR + Deamidated (NQ); Oxidation (M)
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