MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1433 562.8235 1123.6324 1123.6390 -5.80 1 10 0.19 +1Score > 36 indicates identity
Score > 15 indicates homology		 SYGIKPFGKK
2795 587.9515 1760.8327 1760.8662 -19.0 1 10 0.66 +1Score > 40 indicates identity
Score > 21 indicates homology		 RTCLVANTSNMPVAAR + Deamidated (NQ)
3378 1070.0194 2138.0242 2138.0491 -11.6 1 10 0.92 +1Score > 39 indicates identity
Score > 22 indicates homology		 PLLDPNTQTLAESSAAQPQR + 2 Deamidated (NQ)
1590 593.3260 1184.6374 1184.6401 -2.20 0 10 0.63 +1Score > 40 indicates identity
Score > 21 indicates homology		 SQAALILLNNE
4139 930.8053 2789.3941 2789.3429 18.3 0 10 0.3 +1Score > 37 indicates identity
Score > 17 indicates homology		 QINLDSDLLGDARPFLQDGMTVQLE + 2 Deamidated (NQ)
2515 806.9558 1611.8970 1611.8944 1.63 0 10 0.82 +1Score > 37 indicates identity
Score > 22 indicates homology		 DGGISGTVIAIAGVLNR
3372 1068.5627 2135.1108 2135.0858 11.7 1 10 0.36 +1Score > 38 indicates identity
Score > 18 indicates homology		 ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
1737 620.3404 1238.6662 1238.6805 -11.5 0 10 0.86 +1Score > 39 indicates identity
Score > 22 indicates homology		 ALVANMVHGVTK
1920 663.4166 1324.8186 1324.8078 8.18 1 10 0.56 +1Score > 29 indicates identity
Score > 20 indicates homology		 NVVVVAANGKILK + Deamidated (NQ)
2601 826.4419 1650.8692 1650.8689 0.19 0 10 0.4 +1Score > 39 indicates identity
Score > 19 indicates homology		 LTLDDGQVVNAHLTR
3438 725.0671 2172.1795 2172.1579 9.93 0 10 0.93 +1Score > 36 indicates identity
Score > 22 indicates homology		 QPVFAPAPGQVQGIQQALPPK + 2 Deamidated (NQ)
2785 877.4673 1752.9200 1752.8934 15.2 1 10 0.58 +1Score > 39 indicates identity
Score > 20 indicates homology		 LSPETLYLAVNYVDR + Deamidated (NQ)
2947 617.9913 1850.9521 1850.9639 -6.38 1 10 0.42 +1Score > 39 indicates identity
Score > 19 indicates homology		 GKPTDLSIHETAWGLAR
485 343.6678 685.3210 685.3143 9.80 0 10 0.5 +1Score > 37 indicates identity
Score > 19 indicates homology		 NPNGQR + Deamidated (NQ)
2263 752.3917 1502.7688 1502.7437 16.7 1 10 0.9 +1Score > 41 indicates identity
Score > 22 indicates homology		 LTSDGAGISGGQRQR + Deamidated (NQ)
2724 429.4659 1713.8345 1713.8356 -0.63 1 10 0.9 +1Score > 40 indicates identity
Score > 22 indicates homology		 VTLIPCGDGNAVAAARE + Deamidated (NQ)
3763 1190.6470 2379.2794 2379.2885 -3.82 0 10 0.81 +1Score > 35 indicates identity
Score > 22 indicates homology		 FGIGADGVLFLAKPLHTSLHMR
1837 645.8599 1289.7052 1289.7092 -3.04 1 10 0.45 +1Score > 39 indicates identity
Score > 19 indicates homology		 LAVFKANGDSIR
2918 457.2455 1824.9529 1824.9833 -16.7 0 10 0.84 +1Score > 39 indicates identity
Score > 22 indicates homology		 TLVVDGDVNTVLVPIDR + Deamidated (NQ)
507 349.7331 697.4516 697.4486 4.34 1 10 0.96 +1Score > 31 indicates identity
Score > 22 indicates homology		 AAPKLAK
1065 319.8391 956.4955 956.4927 2.89 1 10 0.62 +1Score > 39 indicates identity
Score > 20 indicates homology		 TPVAEDVAR
1411 372.5354 1114.5844 1114.5883 -3.55 1 10 0.93 +1Score > 41 indicates identity
Score > 22 indicates homology		 FASATHKTPR
3285 1043.5388 2085.0630 2085.0466 7.90 1 10 0.29 +1Score > 39 indicates identity
Score > 17 indicates homology		 YHGLSVGVILNTMHPFER + Oxidation (M)
4164 938.4618 2812.3636 2812.3412 7.95 1 10 0.35 +1Score > 37 indicates identity
Score > 18 indicates homology		 IGDRCQLVGDDLFVTNVDFLAMGIE + Oxidation (M)
1474 569.8066 1137.5986 1137.6070 -7.35 0 10 0.81 +1Score > 40 indicates identity
Score > 21 indicates homology		 ADFFLLDGIK
1236 343.5322 1027.5748 1027.5662 8.36 1 10 0.83 +1Score > 40 indicates identity
Score > 22 indicates homology		 KQSGLPQLR + 2 Deamidated (NQ)
1724 619.3142 1236.6138 1236.5916 18.0 0 10 0.93 +1Score > 41 indicates identity
Score > 22 indicates homology		 MLMPCIQLAK + Deamidated (NQ); 2 Oxidation (M)
3751 592.7967 2367.1577 2367.1383 8.20 1 10 0.94 +1Score > 39 indicates identity
Score > 22 indicates homology		 FVDHLQKIDQQYDVFSDIR + 2 Deamidated (NQ)
3261 692.3857 2074.1353 2074.1324 1.41 1 10 0.33 +1Score > 35 indicates identity
Score > 18 indicates homology		 QITPKPIGVGFGIASPEHAR
3001 629.0075 1884.0007 1883.9887 6.34 1 10 0.42 +1Score > 38 indicates identity
Score > 19 indicates homology		 MVSLLNHSLNVTTKDGR
3884 830.4727 2488.3963 2488.4201 -9.57 0 10 0.2 +1Score > 30 indicates identity
Score > 15 indicates homology		 LFLPNIILFVVNILAMMSAVLR + 2 Deamidated (NQ)
3260 688.0435 2061.1087 2061.0677 19.9 1 10 0.21 +1Score > 37 indicates identity
Score > 16 indicates homology		 LPFSIQQRAQTLAMDAVR + Deamidated (NQ); Oxidation (M)
3268 692.7201 2075.1385 2075.1164 10.7 1 10 0.18 +1Score > 35 indicates identity
Score > 15 indicates homology		 QITPKPIGVGFGIASPEHAR + Deamidated (NQ)
3701 774.4219 2320.2439 2320.2096 14.8 1 10 0.95 +1Score > 35 indicates identity
Score > 22 indicates homology		 ASMLVTLANLEQHPLSVPINR + 2 Deamidated (NQ); Oxidation (M)
3835 814.4024 2440.1854 2440.2097 -9.96 1 10 0.22 +1Score > 38 indicates identity
Score > 16 indicates homology		 HATPMLDQLETGPWPSFISGIK + Oxidation (M)
4502 834.4572 3333.7997 3333.7793 6.11 0 10 0.13 +1Score > 32 indicates identity
Score > 14 indicates homology		 VQLINQPAHTAPQLIRPAAPAHLSSDSTSSVK
1385 552.8291 1103.6436 1103.6260 16.0 1 10 0.95 +1Score > 37 indicates identity
Score > 22 indicates homology		 KVVLLGMSLE + Oxidation (M)
1690 409.5463 1225.6171 1225.6244 -5.96 1 10 1 +1Score > 40 indicates identity
Score > 22 indicates homology		 QDKYAIWFR
969 307.8567 920.5483 920.5304 19.4 1 10 0.84 +1Score > 35 indicates identity
Score > 22 indicates homology		 VAHRVSPR
3150 666.0626 1995.1660 1995.1631 1.45 0 10 0.21 +1Score > 28 indicates identity
Score > 16 indicates homology		 IPAGLLVMLLLWLGYLNP
1599 594.8240 1187.6334 1187.6397 -5.29 1 10 0.97 +1Score > 41 indicates identity
Score > 22 indicates homology		 LAAQVDLSKNK + 2 Deamidated (NQ)
3706 775.7208 2324.1406 2324.1136 11.6 0 10 0.43 +1Score > 39 indicates identity
Score > 19 indicates homology		 SILPMSCGVLVMVMSGLLDAAE + 2 Oxidation (M)
1746 415.5682 1243.6828 1243.6884 -4.55 1 10 0.7 +1Score > 40 indicates identity
Score > 21 indicates homology		 ALRATTQDQLK
2216 739.3533 1476.6920 1476.6997 -5.21 0 10 0.84 +1Score > 41 indicates identity
Score > 22 indicates homology		 FFASHVVQNNGVR + 3 Deamidated (NQ)
1707 615.8406 1229.6666 1229.6615 4.15 1 10 0.83 +1Score > 41 indicates identity
Score > 21 indicates homology		 VIATASNGAEAVK
2968 466.7452 1862.9517 1862.9785 -14.4 1 10 0.28 +1Score > 40 indicates identity
Score > 17 indicates homology		 MRILVTNDDGVHAPGLR
1586 394.9015 1181.6827 1181.6656 14.5 1 10 0.76 +1Score > 36 indicates identity
Score > 21 indicates homology		 QIGIPDEGIIK
2618 417.2339 1664.9065 1664.9138 -4.38 1 10 0.67 +1Score > 37 indicates identity
Score > 20 indicates homology		 TVLGFFGITDIEVVR
2612 830.4389 1658.8632 1658.8475 9.49 1 10 0.96 +1Score > 40 indicates identity
Score > 22 indicates homology		 GANATLTVDLASQEIR + Deamidated (NQ)
3929 636.8059 2543.1945 2543.2391 -17.5 1 10 0.79 +1Score > 38 indicates identity
Score > 21 indicates homology		 RTANIFTQITQINTTNDVVSFR + 5 Deamidated (NQ)
4205 718.1182 2868.4437 2868.4354 2.88 1 10 0.49 +1Score > 37 indicates identity
Score > 19 indicates homology		 DYSNKTVVTLSLDDPLNPLLVMLYE + Deamidated (NQ); Oxidation (M)
1343 541.7529 1081.4912 1081.5114 -18.6 1 10 0.9 +1Score > 40 indicates identity
Score > 22 indicates homology		 DLGFMNLEK + Oxidation (M)
3035 638.3117 1911.9133 1911.9003 6.79 1 10 0.55 +1Score > 40 indicates identity
Score > 20 indicates homology		 QPPQFTRGYGLVFGQSE + 2 Deamidated (NQ)
1844 647.3380 1292.6614 1292.6798 -14.2 1 10 0.72 +1Score > 41 indicates identity
Score > 21 indicates homology		 GDMIEAIFILR + Oxidation (M)
2697 568.2877 1701.8413 1701.8495 -4.85 0 10 0.37 +1Score > 40 indicates identity
Score > 18 indicates homology		 IAVGMGADVTVIDLSPE + Oxidation (M)
3176 671.0233 2010.0481 2010.0633 -7.60 1 10 0.83 +1Score > 38 indicates identity
Score > 21 indicates homology		 GQIVDTVVQEQRPSVLLE + Deamidated (NQ)
3669 1153.5521 2305.0896 2305.1122 -9.80 1 10 0.13 +1Score > 39 indicates identity
Score > 13 indicates homology		 MNNLIKAYAAGVMSAAFLFGSE + Deamidated (NQ)
3886 832.4239 2494.2499 2494.2997 -20.0 0 10 0.16 +1Score > 38 indicates identity
Score > 14 indicates homology		 VMLALAIFAALSLAASQVMNGVMR + 2 Deamidated (NQ); Oxidation (M)
1067 479.2564 956.4982 956.4927 5.79 1 10 0.78 +1Score > 39 indicates identity
Score > 21 indicates homology		 TIDRVGPAE
2022 693.8633 1385.7120 1385.7337 -15.6 0 10 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology		 ITLGVIPGMGGSQR + Deamidated (NQ)
3729 1175.5769 2349.1392 2349.1812 -17.9 1 10 0.43 +1Score > 39 indicates identity
Score > 18 indicates homology		 TNPNFNQSVLNTALKTNSSGIK + 2 Deamidated (NQ)
2892 906.4523 1810.8900 1810.8924 -1.28 1 10 0.55 +1Score > 40 indicates identity
Score > 19 indicates homology		 GQELLYAGMPISDVFR + Oxidation (M)
2506 806.9333 1611.8520 1611.8508 0.76 1 10 0.87 +1Score > 39 indicates identity
Score > 21 indicates homology		 EQVPQGYVAPAQVLI + Deamidated (NQ)
3019 632.3524 1894.0354 1894.0352 0.073 1 10 0.96 +1Score > 36 indicates identity
Score > 22 indicates homology		 IAQSINFLLYAPLFRE
3115 986.5361 1971.0576 1971.0425 7.67 1 10 0.61 +1Score > 37 indicates identity
Score > 20 indicates homology		 ADVLSDTRALLSALDFHK
1979 682.3435 1362.6724 1362.6667 4.22 0 10 0.82 +1Score > 41 indicates identity
Score > 21 indicates homology		 DFVAQLVSQNIE + Deamidated (NQ)
2653 837.4689 1672.9232 1672.9069 9.75 1 10 0.37 +1Score > 36 indicates identity
Score > 18 indicates homology		 KLVILGGAMQGNVLDK + 2 Deamidated (NQ); Oxidation (M)
2944 617.2753 1848.8041 1848.7869 9.27 1 10 0.18 +1Score > 37 indicates identity
Score > 15 indicates homology		 KMSGHMGAVQQSTQNLE + 4 Deamidated (NQ)
3186 672.0019 2012.9839 2012.9691 7.34 0 10 0.29 +1Score > 40 indicates identity
Score > 17 indicates homology		 GQVSWALGALSSGGPGTQLPE + 2 Deamidated (NQ)
3102 653.6852 1958.0338 1958.0275 3.21 0 10 0.56 +1Score > 38 indicates identity
Score > 20 indicates homology		 LFPSTHALQPHLSAHFR
3783 799.4221 2395.2445 2395.2707 -11.0 1 10 0.79 +1Score > 37 indicates identity
Score > 21 indicates homology		 ISTAVADGPQSAILEQVTNGVAVR
3581 749.4138 2245.2196 2245.2470 -12.2 1 10 0.15 +1Score > 35 indicates identity
Score > 14 indicates homology		 LSILAPLHNPANLAGIEFVQK + Deamidated (NQ)
1809 639.3721 1276.7296 1276.7292 0.36 1 9 0.35 +1Score > 35 indicates identity
Score > 17 indicates homology		 KALFAPFQVTR
2534 809.4396 1616.8646 1616.8774 -7.87 1 9 0.34 +1Score > 39 indicates identity
Score > 17 indicates homology		 DTIKYLLSVDSVHK
4318 1009.5314 3025.5724 3025.5551 5.71 0 9 0.19 +1Score > 35 indicates identity
Score > 15 indicates homology		 LAWFGFVMPALVLNYFGQGALLLIEPE + 2 Deamidated (NQ); Oxidation (M)
2454 794.4371 1586.8596 1586.8529 4.27 1 9 0.91 +1Score > 39 indicates identity
Score > 22 indicates homology		 LSLTVLGGNRQWSR + Deamidated (NQ)
3402 717.0582 2148.1528 2148.1356 7.99 0 9 0.45 +1Score > 36 indicates identity
Score > 19 indicates homology		 NMGTLALMGLIMTLLAQASAK + Deamidated (NQ)
620 379.2143 756.4140 756.4130 1.39 0 9 0.88 +1Score > 39 indicates identity
Score > 21 indicates homology		 LVASPDR
2523 538.9555 1613.8447 1613.8236 13.1 1 9 0.74 +1Score > 40 indicates identity
Score > 21 indicates homology		 ALGTALGMPDEVVWR
3106 656.3359 1965.9859 1965.9864 -0.25 1 9 0.4 +1Score > 40 indicates identity
Score > 18 indicates homology		 SLMGTVVSNKMNDTVVVR + Deamidated (NQ); Oxidation (M)
2537 809.9984 1617.9822 1617.9566 15.8 1 9 0.16 +1Score > 24 indicates identity
Score > 14 indicates homology		 KLAPVLQPGAQLDLR
389 632.3764 631.3691 631.3653 6.06 1 9 0.92 +1Score > 41 indicates identity
Score > 22 indicates homology		 KSIQR + Deamidated (NQ)
1115 325.1957 972.5653 972.5604 5.03 1 9 0.9 +1Score > 39 indicates identity
Score > 21 indicates homology		 KQIDTLAGK
1880 655.3309 1308.6472 1308.6418 4.20 1 9 0.96 +1Score > 41 indicates identity
Score > 22 indicates homology		 QGTAEMMGLLVK + 2 Oxidation (M)
2047 465.8961 1394.6665 1394.6864 -14.3 1 9 0.77 +1Score > 41 indicates identity
Score > 21 indicates homology		 EMIAAFDLSSVGR
931 452.7645 903.5144 903.5025 13.2 0 9 0.99 +1Score > 39 indicates identity
Score > 22 indicates homology		 LSQVITSR + Deamidated (NQ)
2478 801.9511 1601.8876 1601.8777 6.21 0 9 0.3 +1Score > 37 indicates identity
Score > 17 indicates homology		 LADYPTVAIGGVSLAR
2615 832.4402 1662.8658 1662.8828 -10.2 1 9 0.87 +1Score > 40 indicates identity
Score > 21 indicates homology		 YLVIKDVNDDISAAK
2753 577.3207 1728.9403 1728.9159 14.1 1 9 0.9 +1Score > 38 indicates identity
Score > 21 indicates homology		 QAIGLTGDLPFIGRDR + Deamidated (NQ)
3390 1073.5790 2145.1434 2145.1252 8.51 0 9 0.42 +1Score > 37 indicates identity
Score > 18 indicates homology		 CKPSAPQTLHQILQDPALK + Deamidated (NQ)
2702 427.7358 1706.9141 1706.8951 11.1 1 9 0.75 +1Score > 38 indicates identity
Score > 21 indicates homology		 NAKLNNLNNVNIVHK + 3 Deamidated (NQ)
1360 544.2941 1086.5736 1086.5710 2.45 0 9 0.73 +1Score > 42 indicates identity
Score > 20 indicates homology		 GVPDTPGVAFK
2462 531.9741 1592.9005 1592.9012 -0.44 1 9 0.49 +1Score > 35 indicates identity
Score > 19 indicates homology		 HSVHALLAHPRVTR
2589 550.9561 1649.8465 1649.8600 -8.17 1 9 0.45 +1Score > 40 indicates identity
Score > 18 indicates homology		 ELKPFPSVCATVFR
3240 682.6954 2045.0644 2045.0350 14.3 1 9 0.95 +1Score > 38 indicates identity
Score > 22 indicates homology		 GQTVPLNTQLMTIVELNR + 3 Deamidated (NQ); Oxidation (M)
310 590.3174 589.3101 589.3184 -14.0 0 9 0.91 +1Score > 43 indicates identity
Score > 21 indicates homology		 ATAATR
1186 504.2625 1006.5104 1006.5043 6.11 1 9 0.77 +1Score > 42 indicates identity
Score > 21 indicates homology		 SNTNSRSLK + Deamidated (NQ)
2368 777.4136 1552.8126 1552.8209 -5.33 0 9 0.96 +1Score > 39 indicates identity
Score > 22 indicates homology		 VNVPEPGSSTLLQGR
2628 834.4514 1666.8882 1666.9002 -7.18 1 9 0.94 +1Score > 38 indicates identity
Score > 22 indicates homology		 ANVPILGTQVRDLDR + Deamidated (NQ)
1606 595.8442 1189.6738 1189.6819 -6.77 1 9 0.62 +1Score > 37 indicates identity
Score > 20 indicates homology		 IADIYRGGVVK
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