MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1370 365.5256 1093.5550 1093.5590 -3.69 0 11 0.63 +1Score > 41 indicates identity
Score > 21 indicates homology
IAGVGFINMR + Deamidated (NQ); Oxidation (M)
1950 449.2375 1344.6907 1344.6885 1.63 0 11 0.63 +1Score > 41 indicates identity
Score > 21 indicates homology
LQQATISAQQQK + 2 Deamidated (NQ)
3530 555.0692 2216.2477 2216.2466 0.51 0 11 0.79 +1Score > 31 indicates identity
Score > 22 indicates homology
VMAVGPYVVVGLVIWVCVLK + Oxidation (M)
3123 990.9907 1979.9668 1979.9802 -6.73 0 11 0.34 +1Score > 40 indicates identity
Score > 19 indicates homology
NVDIVIVSIFINPMQFE + 3 Deamidated (NQ)
2100 707.9147 1413.8148 1413.8278 -9.19 0 11 0.57 +1Score > 35 indicates identity
Score > 21 indicates homology
LFLLGRPMLQAR
472 338.2077 674.4008 674.3897 16.5 1 11 0.62 +1Score > 39 indicates identity
Score > 21 indicates homology
AMKAVR
2194 736.3712 1470.7278 1470.7354 -5.15 1 11 0.65 +1Score > 41 indicates identity
Score > 21 indicates homology
AGNTLYEISSFLR + Deamidated (NQ)
2068 351.9375 1403.7209 1403.7310 -7.17 1 11 0.73 +1Score > 41 indicates identity
Score > 22 indicates homology
AGPRPEGYFAALR
2218 493.6088 1477.8046 1477.8327 -19.0 1 11 0.32 +1Score > 38 indicates identity
Score > 18 indicates homology
AIGFLVGQIMKASK + Oxidation (M)
560 361.2170 720.4194 720.4170 3.37 0 11 0.88 +1Score > 38 indicates identity
Score > 23 indicates homology
ASLGFVK
1915 442.2675 1323.7807 1323.7809 -0.16 1 11 0.67 +1Score > 34 indicates identity
Score > 21 indicates homology
GVIAMLARQLPR
1585 394.9011 1181.6815 1181.6590 19.0 1 11 0.31 +1Score > 36 indicates identity
Score > 18 indicates homology
KAISGMIPHTK
1331 537.8050 1073.5954 1073.6081 -11.8 0 11 0.82 +1Score > 40 indicates identity
Score > 22 indicates homology
ITDILTSVGR
1466 567.8036 1133.5926 1133.5937 -0.89 0 11 0.91 +1Score > 42 indicates identity
Score > 23 indicates homology
LMDALMTAIR
1561 586.8513 1171.6880 1171.6924 -3.75 1 11 0.99 +1Score > 36 indicates identity
Score > 23 indicates homology
QATKLTAGIAAK
2256 751.4002 1500.7858 1500.7572 19.1 1 11 0.4 +1Score > 40 indicates identity
Score > 19 indicates homology
LGEAQTVWDNLQK
3059 483.0185 1928.0449 1928.0268 9.37 1 11 0.23 +1Score > 36 indicates identity
Score > 17 indicates homology
RQTFVFSATLALSANFR
3495 550.2746 2197.0693 2197.0434 11.8 0 11 0.68 +1Score > 39 indicates identity
Score > 21 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
3976 862.1192 2583.3358 2583.3553 -7.56 1 11 0.2 +1Score > 37 indicates identity
Score > 16 indicates homology
VDPVNFKLLSQCIQVVLAAHMGK + Deamidated (NQ); Oxidation (M)
897 445.7365 889.4584 889.4505 8.95 0 11 0.99 +1Score > 42 indicates identity
Score > 23 indicates homology
NLLNSSGGK + Deamidated (NQ)
2988 938.9543 1875.8940 1875.8950 -0.49 1 11 1 +1Score > 40 indicates identity
Score > 23 indicates homology
QIEVTTDGAGVSGTVVDVE + Deamidated (NQ)
731 807.4172 806.4099 806.3956 17.7 0 11 0.74 +1Score > 41 indicates identity
Score > 22 indicates homology
QTAAIMR + Deamidated (NQ); Oxidation (M)
2141 479.6110 1435.8112 1435.7857 17.7 0 11 0.81 +1Score > 35 indicates identity
Score > 22 indicates homology
NLVVFTVASAMLR + Oxidation (M)
2499 537.3430 1609.0072 1608.9926 9.03 0 11 0.63 +1Score > 21 indicates identity SISVGKPLSILLLNR
2304 759.9391 1517.8636 1517.8705 -4.49 1 11 0.89 +1Score > 35 indicates identity
Score > 23 indicates homology
ISLVNIKYLVLDE
4022 888.7911 2663.3515 2663.3604 -3.35 1 11 0.26 +1Score > 37 indicates identity
Score > 17 indicates homology
VLYVPSMGFCILVAHGWQKISNK + Deamidated (NQ); Oxidation (M)
2614 831.9574 1661.9002 1661.8923 4.79 1 11 0.19 +1Score > 38 indicates identity
Score > 16 indicates homology
SRSLCNIPFITINK
2989 938.9780 1875.9414 1875.9578 -8.70 1 11 0.68 +1Score > 40 indicates identity
Score > 21 indicates homology
NKTQGNLQDLFLNVLR + 4 Deamidated (NQ)
766 415.6945 829.3744 829.3719 3.12 0 11 0.46 +1Score > 37 indicates identity
Score > 20 indicates homology
DPDAWAR
1206 507.7937 1013.5728 1013.5692 3.62 0 11 0.6 +1Score > 40 indicates identity
Score > 21 indicates homology
VGCISLPLR
2488 804.4320 1606.8494 1606.8467 1.70 1 11 0.75 +1Score > 39 indicates identity
Score > 22 indicates homology
VNYKIANTGNSLWK
3535 1112.0239 2222.0332 2222.0159 7.79 1 11 0.41 +1Score > 39 indicates identity
Score > 19 indicates homology
SSDADVDSPQSRSRPSTSSTR
1793 636.8408 1271.6670 1271.6609 4.86 0 11 0.95 +1Score > 41 indicates identity
Score > 23 indicates homology
NLQQPTTLVQK + 3 Deamidated (NQ)
1951 449.2378 1344.6916 1344.7184 -19.9 1 11 0.6 +1Score > 41 indicates identity
Score > 21 indicates homology
IQAVVDMAKAQR + Oxidation (M)
728 403.2318 804.4490 804.4606 -14.4 1 10 0.85 +1Score > 41 indicates identity
Score > 22 indicates homology
KGFANIR
2518 404.2328 1612.9021 1612.9162 -8.72 1 10 0.56 +1Score > 37 indicates identity
Score > 20 indicates homology
TIVRSDPHIGLLHR
1538 389.5575 1165.6507 1165.6343 14.1 1 10 0.68 +1Score > 37 indicates identity
Score > 21 indicates homology
SGKQQTLIYK + Deamidated (NQ)
2632 556.9799 1667.9179 1667.9094 5.07 0 10 0.64 +1Score > 37 indicates identity
Score > 21 indicates homology
QQGIGTLLTVPGAAGGVK + 2 Deamidated (NQ)
3214 1017.0261 2032.0376 2032.0412 -1.72 1 10 0.87 +1Score > 39 indicates identity
Score > 22 indicates homology
SYAGNPLVNALCVGTLRVE
2930 613.3391 1836.9955 1836.9767 10.2 1 10 0.47 +1Score > 37 indicates identity
Score > 20 indicates homology
AGLGLAQEAPVVALMPGSR + Deamidated (NQ)
867 437.7170 873.4194 873.4344 -17.2 1 10 0.4 +1Score > 41 indicates identity
Score > 19 indicates homology
LKHNFSE
2072 469.2850 1404.8332 1404.8340 -0.61 0 10 0.39 +1Score > 31 indicates identity
Score > 19 indicates homology
LGLGNIAPLPVVNK + Deamidated (NQ)
2243 748.4562 1494.8978 1494.8770 14.0 0 10 0.29 +1Score > 30 indicates identity
Score > 18 indicates homology
LAADVTGVPTLLVAR
3057 964.4909 1926.9672 1926.9774 -5.29 1 10 0.79 +1Score > 39 indicates identity
Score > 22 indicates homology
WSGYAFGMGVDRTVLLR
3145 996.0229 1990.0312 1990.0306 0.34 1 10 0.43 +1Score > 39 indicates identity
Score > 19 indicates homology
TVNQLISFMNGNTAPVRK + Deamidated (NQ)
3265 692.6913 2075.0521 2075.0826 -14.7 1 10 0.85 +1Score > 39 indicates identity
Score > 22 indicates homology
IAFAFQVDIPLNLDQLKE + 2 Deamidated (NQ)
3466 729.3997 2185.1773 2185.1637 6.22 1 10 0.56 +1Score > 36 indicates identity
Score > 20 indicates homology
SVTAAARSINLSQPAMSAAIAR
2261 501.9286 1502.7640 1502.7841 -13.4 1 10 0.67 +1Score > 41 indicates identity
Score > 21 indicates homology
FINVVGSPDTGNKR
2321 765.4343 1528.8540 1528.8402 9.06 0 10 0.36 +1Score > 37 indicates identity
Score > 18 indicates homology
AFNIDLGFPLIGPR
2444 792.4188 1582.8230 1582.8025 13.0 0 10 0.44 +1Score > 40 indicates identity
Score > 19 indicates homology
QISLQQQLIHVME + Deamidated (NQ); Oxidation (M)
2458 531.2811 1590.8215 1590.8253 -2.43 0 10 0.84 +1Score > 40 indicates identity
Score > 22 indicates homology
LSVDGVISALAFDQR + Deamidated (NQ)
3143 996.0222 1990.0298 1990.0081 10.9 1 10 0.71 +1Score > 39 indicates identity
Score > 21 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
479 342.2260 682.4374 682.4378 -0.46 0 10 0.56 +1Score > 35 indicates identity
Score > 20 indicates homology
LGGPVLK
2048 349.9469 1395.7585 1395.7796 -15.1 0 10 0.6 +1Score > 38 indicates identity
Score > 21 indicates homology
MLDHAVLLQVIK + Deamidated (NQ); Oxidation (M)
3092 488.7513 1950.9761 1950.9660 5.17 1 10 0.84 +1Score > 40 indicates identity
Score > 22 indicates homology
SNRHIYAQVIDDVHQR + Deamidated (NQ)
1566 587.2887 1172.5628 1172.5713 -7.24 0 10 0.98 +1Score > 40 indicates identity
Score > 23 indicates homology
LQLLQQDWE + Deamidated (NQ)
1720 617.3170 1232.6194 1232.6295 -8.17 1 10 0.95 +1Score > 42 indicates identity
Score > 23 indicates homology
SQIQNKASAMR
2300 758.3738 1514.7330 1514.7035 19.5 1 10 0.55 +1Score > 41 indicates identity
Score > 20 indicates homology
SESMYQSQLLASR + Oxidation (M)
2971 933.5040 1864.9934 1864.9829 5.66 1 10 0.68 +1Score > 38 indicates identity
Score > 21 indicates homology
GMKVPDVLLSGNHAAIAR + Deamidated (NQ); Oxidation (M)
293 572.3136 571.3063 571.3078 -2.56 0 10 0.62 +1Score > 35 indicates identity
Score > 21 indicates homology
AAAPSR
644 382.7133 763.4120 763.4228 -14.1 0 10 0.51 +1Score > 41 indicates identity
Score > 20 indicates homology
NAVAVYK
4396 1055.5167 3163.5283 3163.5282 0.014 1 10 0.15 +1Score > 36 indicates identity
Score > 15 indicates homology
LAATFQSDIPSKQNQAQNGNQSHLPPQSR + 2 Deamidated (NQ)
3172 670.9871 2009.9395 2009.9402 -0.36 1 10 0.7 +1Score > 39 indicates identity
Score > 21 indicates homology
SHRNAILQANQQDLDAGR + 4 Deamidated (NQ)
1843 647.3225 1292.6304 1292.6248 4.33 0 10 0.7 +1Score > 41 indicates identity
Score > 21 indicates homology
ISVSGVFGINNGE + Deamidated (NQ)
4072 903.8201 2708.4385 2708.4346 1.43 1 10 0.98 +1Score > 35 indicates identity
Score > 23 indicates homology
RLMAYSTILNIGYVLIGVILPNQE + 3 Deamidated (NQ); Oxidation (M)
3009 630.0103 1887.0091 1886.9989 5.37 0 10 0.51 +1Score > 38 indicates identity
Score > 20 indicates homology
VIGINTGNLGFLTDLNPK + 2 Deamidated (NQ)
2626 834.4484 1666.8822 1666.8890 -4.04 1 10 0.86 +1Score > 39 indicates identity
Score > 22 indicates homology
SIVGEIVQNLINDPR + Deamidated (NQ)
2820 887.4672 1772.9198 1772.8866 18.8 0 10 0.81 +1Score > 40 indicates identity
Score > 22 indicates homology
LNTILNTMSTIYSTGK + Deamidated (NQ); Oxidation (M)
3386 714.7255 2141.1547 2141.1692 -6.77 0 10 0.54 +1Score > 36 indicates identity
Score > 20 indicates homology
IQQQLTTILSTAQQGQLLR + 2 Deamidated (NQ)
3642 765.3945 2293.1617 2293.1240 16.4 1 10 0.74 +1Score > 38 indicates identity
Score > 21 indicates homology
FWLGNDHIHALTAQGTNELR + Deamidated (NQ)
280 557.3680 556.3607 556.3585 4.07 0 10 0.99 +1Score > 37 indicates identity
Score > 23 indicates homology
VVVVAA
2701 569.9534 1706.8384 1706.8264 7.03 1 10 0.47 +1Score > 41 indicates identity
Score > 19 indicates homology
RQTFALFSNPGNPQK + 3 Deamidated (NQ)
2945 925.4546 1848.8946 1848.9026 -4.32 1 10 0.23 +1Score > 40 indicates identity
Score > 16 indicates homology
GQLSSVTAVLQEMNLLE + 2 Deamidated (NQ); Oxidation (M)
3236 681.6780 2042.0122 2042.0030 4.47 0 10 0.72 +1Score > 39 indicates identity
Score > 21 indicates homology
QTLTFYMGVAGLPTIQQR + 3 Deamidated (NQ); Oxidation (M)
3316 1055.5573 2109.1000 2109.1106 -5.01 1 10 0.69 +1Score > 38 indicates identity
Score > 21 indicates homology
AGQDIQEVIDNLPINVPFK
2116 712.3604 1422.7062 1422.7242 -12.6 1 10 0.88 +1Score > 41 indicates identity
Score > 22 indicates homology
ILGQSLSNAYVEK + 2 Deamidated (NQ)
3449 1089.5278 2177.0410 2177.0384 1.20 0 10 0.22 +1Score > 39 indicates identity
Score > 16 indicates homology
FADMAGAITALPVTMQLDAPE + Oxidation (M)
478 341.6987 681.3828 681.3810 2.76 0 10 0.41 +1Score > 37 indicates identity
Score > 19 indicates homology
IDVHAK
1357 362.8776 1085.6110 1085.6193 -7.65 1 10 0.93 +1Score > 40 indicates identity
Score > 22 indicates homology
KPKSNSAVAGK
2938 922.9529 1843.8912 1843.9078 -8.96 0 10 0.57 +1Score > 40 indicates identity
Score > 20 indicates homology
SLGTQFQPHPRPHNAR + 2 Deamidated (NQ)
3918 842.7889 2525.3449 2525.3347 4.03 0 10 0.4 +1Score > 35 indicates identity
Score > 19 indicates homology
MLMFPYILNLLAAMLLGALIGAE + 3 Oxidation (M)
1703 410.5771 1228.7095 1228.7067 2.26 1 10 0.95 +1Score > 38 indicates identity
Score > 22 indicates homology
KLAPAIIFIDE
2071 703.3411 1404.6676 1404.6779 -7.31 1 10 0.34 +1Score > 41 indicates identity
Score > 18 indicates homology
AAARMADDLASAAR + Oxidation (M)
1643 403.1977 1206.5713 1206.5880 -13.9 1 10 0.82 +1Score > 41 indicates identity
Score > 22 indicates homology
QYDAELQALR + Deamidated (NQ)
2000 457.9160 1370.7262 1370.7266 -0.32 1 10 0.28 +1Score > 40 indicates identity
Score > 17 indicates homology
EGRPSTNATLVAR
2262 752.3916 1502.7686 1502.7477 13.9 1 10 0.92 +1Score > 41 indicates identity
Score > 22 indicates homology
NSIHSIYTELNGR
2780 582.2907 1743.8503 1743.8639 -7.79 1 10 0.9 +1Score > 40 indicates identity
Score > 22 indicates homology
VTKNSIAPVSSNPSANR + 3 Deamidated (NQ)
2232 745.9316 1489.8486 1489.8578 -6.16 0 10 0.88 +1Score > 35 indicates identity
Score > 22 indicates homology
LFLLMLSVSGIGPK + Oxidation (M)
3112 657.3766 1969.1080 1969.0997 4.22 1 10 0.2 +1Score > 34 indicates identity
Score > 16 indicates homology
VTYGRIPAGSVVVAGNLPAK + Deamidated (NQ)
2530 539.2889 1614.8449 1614.8174 17.0 1 10 0.77 +1Score > 40 indicates identity
Score > 21 indicates homology
KLNINLAMSPLPSSE + 2 Deamidated (NQ)
866 437.2629 872.5112 872.4967 16.7 1 10 0.53 +1Score > 40 indicates identity
Score > 20 indicates homology
QIAAEITK
1202 506.7423 1011.4700 1011.4695 0.51 1 10 0.89 +1Score > 40 indicates identity
Score > 22 indicates homology
KSGAVAMFGE + Oxidation (M)
1692 410.2270 1227.6592 1227.6499 7.53 0 10 0.89 +1Score > 41 indicates identity
Score > 22 indicates homology
QTLFQTYSLK
746 411.2444 820.4742 820.4695 5.85 0 10 0.5 +1Score > 37 indicates identity
Score > 20 indicates homology
IFDVISK
938 455.2264 908.4382 908.4491 -11.9 0 10 0.85 +1Score > 41 indicates identity
Score > 22 indicates homology
LSLNGYLE + Deamidated (NQ)
3420 720.0580 2157.1522 2157.1178 15.9 1 10 0.99 +1Score > 37 indicates identity
Score > 23 indicates homology
VSNRDAAQLVASDLGFALGPR + Deamidated (NQ)
3629 1141.5992 2281.1838 2281.2066 -9.99 0 10 0.82 +1Score > 38 indicates identity
Score > 22 indicates homology
SAVADGPHSVILNQVTYGIAIR + Deamidated (NQ)
2114 474.9373 1421.7901 1421.7813 6.17 0 10 0.7 +1Score > 37 indicates identity
Score > 21 indicates homology
AKPLMPGGVNSPVR
1405 557.2666 1112.5186 1112.5397 -18.9 1 10 0.61 +1Score > 40 indicates identity
Score > 20 indicates homology
KGVDGNMHVR + Deamidated (NQ)
1301 353.8621 1058.5645 1058.5760 -10.9 0 10 0.78 +1Score > 42 indicates identity
Score > 22 indicates homology
GNPVIGVFQK + Deamidated (NQ)
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