MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3933 848.7721 2543.2945 2543.2941 0.13 1 12 0.91 +1Score > 38 indicates identity
Score > 24 indicates homology
GVFMKDIGVVNAPSGAPTLALTGGAAE + Deamidated (NQ)
1054 475.7538 949.4930 949.4981 -5.34 0 11 0.95 +1Score > 42 indicates identity
Score > 24 indicates homology
GFNVSSIAR
2344 772.4105 1542.8064 1542.8140 -4.92 0 11 0.68 +1Score > 40 indicates identity
Score > 22 indicates homology
LAIQNSQIASAIALE + 2 Deamidated (NQ)
2819 591.9731 1772.8975 1772.8866 6.15 1 11 0.79 +1Score > 40 indicates identity
Score > 23 indicates homology
NDMVENIVNQLIQLK + 3 Deamidated (NQ)
3053 642.3136 1923.9190 1923.9425 -12.2 1 11 1 +1Score > 40 indicates identity
Score > 24 indicates homology
SNDEIHLSSVISTTPPNL + Deamidated (NQ)
936 454.7280 907.4414 907.4399 1.66 1 11 0.62 +1Score > 42 indicates identity
Score > 22 indicates homology
EVVNSFGR + Deamidated (NQ)
2103 472.9413 1415.8021 1415.8236 -15.2 0 11 0.96 +1Score > 37 indicates identity
Score > 24 indicates homology
TVTGTLGTIVDIVK
1104 324.8392 971.4958 971.5036 -8.06 1 11 0.89 +1Score > 41 indicates identity
Score > 23 indicates homology
QVKSLSSHS
1224 510.3036 1018.5926 1018.5811 11.3 1 11 0.76 +1Score > 37 indicates identity
Score > 23 indicates homology
KDFDLLLR
3252 687.3478 2059.0216 2059.0349 -6.48 1 11 0.93 +1Score > 39 indicates identity
Score > 24 indicates homology
HGRMYLLALDPYYYLR + Oxidation (M)
1509 1156.6245 1155.6172 1155.6247 -6.50 0 11 0.79 +1Score > 40 indicates identity
Score > 23 indicates homology
KPNAITNAGLR + 2 Deamidated (NQ)
3521 737.3942 2209.1608 2209.1895 -13.0 1 11 0.57 +1Score > 37 indicates identity
Score > 21 indicates homology
FKVVLNQLINNANDYLGFK
1160 332.8553 995.5441 995.5440 0.066 1 11 0.56 +1Score > 37 indicates identity
Score > 21 indicates homology
WTYKTGLK
1894 439.8840 1316.6302 1316.6071 17.5 0 11 0.95 +1Score > 41 indicates identity
Score > 24 indicates homology
MTHQFPPLTTE + Oxidation (M)
3501 734.7112 2201.1118 2201.0926 8.71 1 11 0.94 +1Score > 39 indicates identity
Score > 24 indicates homology
IFVSSMNRVTVVFTDVQLE + 2 Deamidated (NQ); Oxidation (M)
4187 948.5522 2842.6348 2842.5891 16.1 1 11 1 +1Score > 26 indicates identity
Score > 24 indicates homology
IIITWSRGSTIIPIMIGHTIAIHNGK + Deamidated (NQ)
1375 549.2790 1096.5434 1096.5474 -3.63 0 11 0.69 +1Score > 40 indicates identity
Score > 22 indicates homology
QTLALMFQK + 2 Deamidated (NQ); Oxidation (M)
1613 597.8596 1193.7046 1193.6880 13.9 1 11 0.94 +1Score > 33 indicates identity
Score > 24 indicates homology
PRTVPIQDLR
1784 633.8831 1265.7516 1265.7343 13.7 0 11 0.82 +1Score > 33 indicates identity
Score > 23 indicates homology
LQLQQLGLVPR + 2 Deamidated (NQ)
3428 722.6946 2165.0620 2165.0688 -3.13 1 11 0.53 +1Score > 39 indicates identity
Score > 21 indicates homology
MAAERPQNLQDTFLNFVR + Oxidation (M)
2075 703.8948 1405.7750 1405.7790 -2.78 1 11 0.61 +1Score > 37 indicates identity
Score > 22 indicates homology
APARGAAPVNNLQK
2624 556.6266 1666.8580 1666.8777 -11.9 1 11 0.24 +1Score > 39 indicates identity
Score > 18 indicates homology
ITATSVPPIINIEGNE
875 440.2521 878.4896 878.4861 3.99 1 11 0.99 +1Score > 39 indicates identity
Score > 24 indicates homology
KAFSDAIK
1309 354.8589 1061.5549 1061.5692 -13.5 1 11 0.83 +1Score > 41 indicates identity
Score > 23 indicates homology
FHGMSIVKK + Oxidation (M)
1702 410.5761 1228.7065 1228.7139 -6.06 1 11 0.84 +1Score > 38 indicates identity
Score > 23 indicates homology
QTIDKAIATLR
2532 539.5994 1615.7764 1615.7664 6.14 1 11 0.78 +1Score > 40 indicates identity
Score > 23 indicates homology
LEDHMGAFTVEPVR + Oxidation (M)
2835 893.4612 1784.9078 1784.9277 -11.1 1 11 0.91 +1Score > 40 indicates identity
Score > 23 indicates homology
SRQVLLLQMPLRPGC + 2 Deamidated (NQ); Oxidation (M)
4156 702.8947 2807.5497 2807.5605 -3.86 1 11 0.14 +1Score > 31 indicates identity
Score > 15 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
2189 735.3730 1468.7314 1468.7158 10.7 0 11 0.65 +1Score > 40 indicates identity
Score > 22 indicates homology
APQGSQTHQVSLSK + 2 Deamidated (NQ)
3762 1190.6466 2379.2786 2379.2885 -4.16 0 11 0.91 +1Score > 35 indicates identity
Score > 23 indicates homology
FGIGADGVLFLAKPLHTSLHMR
1981 455.2505 1362.7297 1362.7116 13.2 1 11 0.37 +1Score > 40 indicates identity
Score > 19 indicates homology
EPQTKPSRHQR
831 429.2232 856.4318 856.4290 3.27 0 11 0.46 +1Score > 39 indicates identity
Score > 20 indicates homology
AGVGVSAAPE
1306 531.7680 1061.5214 1061.5175 3.72 0 11 0.84 +1Score > 42 indicates identity
Score > 23 indicates homology
AAIIMNAGQR + 2 Deamidated (NQ); Oxidation (M)
2606 414.9655 1655.8329 1655.8076 15.3 0 11 0.49 +1Score > 40 indicates identity
Score > 21 indicates homology
SNSVISMGVFIGQSTK + 2 Deamidated (NQ)
2784 877.4672 1752.9198 1752.9159 2.27 1 11 0.59 +1Score > 39 indicates identity
Score > 21 indicates homology
KGQVINLDQLHSSWK + Deamidated (NQ)
1235 343.5318 1027.5736 1027.5550 18.1 1 11 0.87 +1Score > 40 indicates identity
Score > 23 indicates homology
VEATPGDVLK
1260 518.3012 1034.5878 1034.6012 -12.9 1 11 0.96 +1Score > 37 indicates identity
Score > 24 indicates homology
IEIYIVGTK
1007 469.2415 936.4684 936.4665 2.09 1 11 0.85 +1Score > 41 indicates identity
Score > 23 indicates homology
VISGEGYGR
2074 469.5989 1405.7749 1405.7929 -12.8 0 11 0.46 +1Score > 37 indicates identity
Score > 20 indicates homology
ITPLVPVAASGQPR + Deamidated (NQ)
2476 533.9672 1598.8798 1598.8992 -12.1 1 11 0.62 +1Score > 38 indicates identity
Score > 22 indicates homology
SKLGNGSGAGGILDLLK
708 399.2308 796.4470 796.4443 3.44 0 11 0.3 +1Score > 36 indicates identity
Score > 18 indicates homology
GAPAVTPGK
1877 437.2221 1308.6445 1308.6449 -0.31 1 11 0.86 +1Score > 41 indicates identity
Score > 23 indicates homology
DLQTEINSFLK + 2 Deamidated (NQ)
4090 911.4605 2731.3597 2731.3660 -2.30 1 11 0.18 +1Score > 38 indicates identity
Score > 16 indicates homology
NMILDMQIGVLVSTLIDEGSQNLVE + Deamidated (NQ)
372 314.2144 626.4142 626.4115 4.36 1 11 0.83 +1Score > 27 indicates identity
Score > 23 indicates homology
KAPIAK
241 506.2669 505.2596 505.2496 19.8 0 11 0.26 +1Score > 44 indicates identity
Score > 18 indicates homology
SSAGGK
2292 379.2159 1512.8345 1512.8260 5.61 0 11 0.78 +1Score > 38 indicates identity
Score > 22 indicates homology
LASNVNLSLGVVDGR
3559 746.4238 2236.2496 2236.2150 15.4 1 11 0.095 +1Score > 33 indicates identity
Score > 13 indicates homology
MFLQARAALVTGGALYIVGNR + Oxidation (M)
2903 606.6308 1816.8706 1816.8917 -11.6 0 11 0.43 +1Score > 40 indicates identity
Score > 20 indicates homology
INTLYGINMTFVTNSK + 2 Deamidated (NQ)
3392 716.0567 2145.1483 2145.1695 -9.89 1 11 0.47 +1Score > 36 indicates identity
Score > 20 indicates homology
LFTRPGLKAAVVNLDDGFGR
868 437.7178 873.4210 873.4344 -15.3 1 11 0.47 +1Score > 42 indicates identity
Score > 20 indicates homology
LKHNFSE
1448 565.8253 1129.6360 1129.6343 1.58 1 11 0.74 +1Score > 39 indicates identity
Score > 22 indicates homology
TLTEGLLNIR + Deamidated (NQ)
1527 580.3621 1158.7096 1158.7046 4.37 1 11 0.18 +1Score > 31 indicates identity
Score > 16 indicates homology
SLPLLMITKK + Oxidation (M)
1806 426.5753 1276.7041 1276.6809 18.1 1 11 0.89 +1Score > 38 indicates identity
Score > 23 indicates homology
ASVTAAMVKDLR + Oxidation (M)
4005 659.8143 2635.2281 2635.2186 3.59 1 11 0.56 +1Score > 37 indicates identity
Score > 21 indicates homology
GQTVFMGDFPLMTDKGTFIINGTE + Deamidated (NQ); Oxidation (M)
2497 805.4571 1608.8996 1608.8723 17.0 1 11 0.16 +1Score > 36 indicates identity
Score > 15 indicates homology
ALGVNDPVQLAILER + 2 Deamidated (NQ)
3822 809.4221 2425.2445 2425.2246 8.18 1 11 0.15 +1Score > 38 indicates identity
Score > 15 indicates homology
AKNVVGLSAMNFGTFPMVNGLSR + Oxidation (M)
1936 668.8304 1335.6462 1335.6531 -5.15 1 11 0.66 +1Score > 41 indicates identity
Score > 22 indicates homology
TDQYRQVSSPR
3373 712.7120 2135.1142 2135.0833 14.4 1 11 0.24 +1Score > 38 indicates identity
Score > 17 indicates homology
LVHHDIIDALENMSRPFK + Deamidated (NQ)
2507 806.9336 1611.8526 1611.8409 7.26 1 11 0.73 +1Score > 39 indicates identity
Score > 22 indicates homology
RTVPVTPLDDWWK
1668 609.8095 1217.6044 1217.5962 6.81 0 11 0.68 +1Score > 42 indicates identity
Score > 22 indicates homology
QGLANLMDDLK + Deamidated (NQ)
545 354.7069 707.3992 707.3966 3.73 0 11 0.89 +1Score > 39 indicates identity
Score > 23 indicates homology
GAAAPPPK
1819 427.5938 1279.7596 1279.7798 -15.8 1 11 0.37 +1Score > 33 indicates identity
Score > 19 indicates homology
LGRKPIIVCPK
2777 581.6678 1741.9816 1741.9475 19.6 1 11 0.56 +1Score > 35 indicates identity
Score > 21 indicates homology
QVVLGGTSKYLINGHR + Deamidated (NQ)
619 377.7519 753.4892 753.4861 4.20 1 11 0.46 +1Score > 32 indicates identity
Score > 20 indicates homology
LGKLPAR
554 358.7010 715.3874 715.3864 1.40 1 11 0.92 +1Score > 44 indicates identity
Score > 23 indicates homology
AVENGVK
1289 527.3087 1052.6028 1052.6091 -5.90 1 11 0.96 +1Score > 36 indicates identity
Score > 23 indicates homology
LVANRVRPE
1571 589.2985 1176.5824 1176.5631 16.4 1 11 0.5 +1Score > 42 indicates identity
Score > 20 indicates homology
SPMAEIVCVR + Oxidation (M)
1975 453.5798 1357.7176 1357.7275 -7.32 1 11 0.81 +1Score > 40 indicates identity
Score > 23 indicates homology
AMGLEAGLVGQLAK + Deamidated (NQ)
3093 651.3342 1950.9808 1950.9660 7.57 1 11 0.4 +1Score > 40 indicates identity
Score > 19 indicates homology
SNRHIYAQVIDDVHQR + Deamidated (NQ)
2745 863.9955 1725.9764 1725.9512 14.6 0 11 0.54 +1Score > 35 indicates identity
Score > 21 indicates homology
ALIAQASSNLIVGNIIE + Deamidated (NQ)
3558 746.4088 2236.2046 2236.2150 -4.68 1 11 0.33 +1Score > 36 indicates identity
Score > 19 indicates homology
MFLQARAALVTGGALYIVGNR + Oxidation (M)
4437 1070.8842 3209.6308 3209.5743 17.6 1 11 1 +1Score > 35 indicates identity
Score > 23 indicates homology
IGNIAMHIHTARNNAGDMTQTLDITTMIR + Deamidated (NQ)
2266 752.3972 1502.7798 1502.7828 -1.94 1 11 0.63 +1Score > 41 indicates identity
Score > 21 indicates homology
LTSDLAVNAAKSAIE + Deamidated (NQ)
2675 846.8741 1691.7336 1691.7373 -2.18 1 11 0.85 +1Score > 37 indicates identity
Score > 23 indicates homology
QLANGVQATLDNSETE + 3 Deamidated (NQ)
1672 610.3288 1218.6430 1218.6390 3.32 1 11 0.51 +1Score > 41 indicates identity
Score > 21 indicates homology
AALASMQRLNK + Deamidated (NQ); Oxidation (M)
3012 946.4802 1890.9458 1890.9548 -4.72 1 11 0.42 +1Score > 40 indicates identity
Score > 20 indicates homology
QQGTGAVAHIQLAGESAPR + Deamidated (NQ)
130 404.2145 403.2072 403.2067 1.31 0 11 0.8 +1Score > 46 indicates identity
Score > 22 indicates homology
VSAAG
2358 775.4043 1548.7940 1548.8004 -4.08 1 11 0.92 +1Score > 40 indicates identity
Score > 23 indicates homology
ISPMKMLAVTGSNGK + Oxidation (M)
2239 498.9556 1493.8450 1493.8453 -0.24 0 11 0.66 +1Score > 35 indicates identity
Score > 22 indicates homology
GDLLVLNGDVPLLR + Deamidated (NQ)
3039 638.9907 1913.9503 1913.9703 -10.5 1 11 0.64 +1Score > 40 indicates identity
Score > 21 indicates homology
VSPAIGNMLMQGLADLER
3255 687.3580 2059.0522 2059.0143 18.4 1 11 0.71 +1Score > 39 indicates identity
Score > 22 indicates homology
LMINTSTNEPDILVDEVR + Deamidated (NQ)
1097 323.8389 968.4949 968.4927 2.25 1 11 0.81 +1Score > 39 indicates identity
Score > 22 indicates homology
EHLDTLNK
2492 804.8942 1607.7738 1607.8042 -18.9 1 11 0.69 +1Score > 41 indicates identity
Score > 22 indicates homology
NKLANISQDQFLSK + 3 Deamidated (NQ)
3329 1058.5392 2115.0638 2115.0847 -9.87 1 11 0.81 +1Score > 39 indicates identity
Score > 22 indicates homology
ALANDDLQLAENLLGKPYR + 2 Deamidated (NQ)
3376 712.7419 2135.2039 2135.2201 -7.62 1 11 0.34 +1Score > 32 indicates identity
Score > 19 indicates homology
DLALVTRQLATLVQAALPIE + Deamidated (NQ)
2015 691.8856 1381.7566 1381.7565 0.096 1 11 0.23 +1Score > 38 indicates identity
Score > 17 indicates homology
DILIEQVQTAPR
3323 704.3757 2110.1053 2110.1456 -19.1 0 11 0.87 +1Score > 38 indicates identity
Score > 23 indicates homology
GIVLLDSQIDHTTGLLMLR + Oxidation (M)
947 457.7767 913.5388 913.5233 17.1 0 11 0.62 +1Score > 40 indicates identity
Score > 21 indicates homology
GNLNVVAVK + Deamidated (NQ)
2902 606.6308 1816.8706 1816.8632 4.08 0 11 0.44 +1Score > 40 indicates identity
Score > 20 indicates homology
NAVHSWVAATLDYLQE + Deamidated (NQ)
433 333.6777 665.3408 665.3384 3.67 0 11 0.91 +1Score > 41 indicates identity
Score > 23 indicates homology
NAYGLK + Deamidated (NQ)
2383 780.9455 1559.8764 1559.8527 15.2 1 11 0.79 +1Score > 36 indicates identity
Score > 22 indicates homology
KSLCANILMIKPR + Deamidated (NQ); Oxidation (M)
2691 567.6342 1699.8808 1699.8628 10.6 1 11 0.38 +1Score > 40 indicates identity
Score > 19 indicates homology
LDEISNDIANVIINR + 2 Deamidated (NQ)
3138 994.5243 1987.0340 1987.0302 1.93 0 11 0.27 +1Score > 39 indicates identity
Score > 18 indicates homology
SGQPSPVNFPFLLNLNLK + 3 Deamidated (NQ)
3668 769.0394 2304.0964 2304.1170 -8.97 1 11 0.14 +1Score > 39 indicates identity
Score > 15 indicates homology
VFMGDFPLMTTKGTFIINGAE + Oxidation (M)
4064 676.6379 2702.5225 2702.4690 19.8 1 11 0.14 +1Score > 29 indicates identity
Score > 15 indicates homology
GVIVRPVANYGMPNHLRITIGLPAE + Oxidation (M)
4063 676.6379 2702.5225 2702.4690 19.8 1 11 1 +1Score > 29 indicates identity
Score > 23 indicates homology
GVIVRPVANYGMPNHLRITIGLPAE + Oxidation (M)
3732 784.3979 2350.1719 2350.1329 16.6 1 11 0.32 +1Score > 39 indicates identity
Score > 18 indicates homology
HQDSSEYSIVVLGQQGVDFLK + 2 Deamidated (NQ)
1120 488.7845 975.5544 975.5542 0.27 0 11 0.95 +1Score > 39 indicates identity
Score > 23 indicates homology
IYIVGFHK
3250 686.3692 2056.0858 2056.0550 14.9 0 11 0.36 +1Score > 38 indicates identity
Score > 19 indicates homology
YLQDQLIIFMALASGISR + 2 Deamidated (NQ); Oxidation (M)
3311 526.5472 2102.1597 2102.1194 19.2 0 11 0.17 +1Score > 34 indicates identity
Score > 16 indicates homology
MVQPDPPANLVVSAIPGKPR + Deamidated (NQ); Oxidation (M)
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