MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2625 556.6273 1666.8601 1666.8501 6.00 1 12 0.75 +1Score > 39 indicates identity
Score > 24 indicates homology
MFASIAPRYDLLNR + Deamidated (NQ)
3524 553.5361 2210.1153 2210.0789 16.4 1 12 0.75 +1Score > 39 indicates identity
Score > 24 indicates homology
NNAACYLPEISQLLNHVPR + 2 Deamidated (NQ)
1986 683.8421 1365.6696 1365.6524 12.6 1 12 0.71 +1Score > 41 indicates identity
Score > 23 indicates homology
TANATALGYEQAR + Deamidated (NQ)
1487 571.8620 1141.7094 1141.7070 2.10 0 12 0.74 +1Score > 30 indicates identity
Score > 24 indicates homology
ISIQVLQTLK
1827 643.3618 1284.7090 1284.6925 12.9 1 12 0.94 +1Score > 39 indicates identity
Score > 25 indicates homology
LLAEDIQNAGIK + Deamidated (NQ)
1836 645.8593 1289.7040 1289.7078 -2.94 0 12 0.59 +1Score > 39 indicates identity
Score > 23 indicates homology
ISIITSQVTLSE
4042 896.1078 2685.3016 2685.2956 2.24 0 12 0.25 +1Score > 38 indicates identity
Score > 19 indicates homology
APNIDGSDLHSAVIITWINMSTSNK + 2 Deamidated (NQ)
917 449.2813 896.5480 896.5443 4.15 0 12 0.43 +1Score > 34 indicates identity
Score > 21 indicates homology
AGLLSAPLR
945 305.1642 912.4708 912.4526 20.0 1 12 0.46 +1Score > 39 indicates identity
Score > 21 indicates homology
SHSRSPSR
2013 690.3372 1378.6598 1378.6802 -14.8 0 12 0.37 +1Score > 41 indicates identity
Score > 21 indicates homology
MSGFQINLNPLK + 2 Deamidated (NQ); Oxidation (M)
3781 599.5563 2394.1961 2394.1525 18.2 0 12 0.56 +1Score > 39 indicates identity
Score > 22 indicates homology
TLWVVVNSNLGLCGGYNANVNK + 3 Deamidated (NQ)
1854 649.3499 1296.6852 1296.6898 -3.53 1 12 0.27 +1Score > 40 indicates identity
Score > 19 indicates homology
NVTPNNNIRQK
2335 512.9846 1535.9320 1535.9511 -12.5 1 12 0.41 +1Score > 28 indicates identity
Score > 21 indicates homology
AVIQALIARGALLAR + Deamidated (NQ)
2536 540.2943 1617.8611 1617.8661 -3.08 0 12 0.57 +1Score > 39 indicates identity
Score > 22 indicates homology
MPHILILNGPNLNR + Deamidated (NQ); Oxidation (M)
1445 565.7777 1129.5408 1129.5516 -9.52 1 12 0.77 +1Score > 41 indicates identity
Score > 24 indicates homology
LAQVRHDYE
1928 665.8696 1329.7246 1329.7187 4.49 1 12 0.62 +1Score > 40 indicates identity
Score > 23 indicates homology
LRSVLNCALAGR + Deamidated (NQ)
3439 725.0746 2172.2020 2172.1711 14.2 1 12 0.16 +1Score > 34 indicates identity
Score > 17 indicates homology
VDTALDVLLELIVLNASAMR + Deamidated (NQ); Oxidation (M)
2759 866.8900 1731.7654 1731.7807 -8.83 0 12 0.73 +1Score > 39 indicates identity
Score > 23 indicates homology
NDNPLVDMQAMSPTAK + Deamidated (NQ)
2985 625.9717 1874.8933 1874.8832 5.35 1 12 0.79 +1Score > 40 indicates identity
Score > 24 indicates homology
FGGNGELSGHNLGNLMLK + 2 Deamidated (NQ); Oxidation (M)
593 367.7153 733.4160 733.4123 5.16 0 12 0.99 +1Score > 39 indicates identity
Score > 25 indicates homology
IADIFR
2992 627.3398 1878.9976 1878.9772 10.8 1 12 0.2 +1Score > 38 indicates identity
Score > 18 indicates homology
ALIASSNNAHIRGNQASR
3008 944.5106 1887.0066 1886.9706 19.1 1 12 0.75 +1Score > 38 indicates identity
Score > 24 indicates homology
QVVMLRLLSDMAQDPR + Oxidation (M)
3171 670.9867 2009.9383 2009.9252 6.51 0 12 0.98 +1Score > 39 indicates identity
Score > 25 indicates homology
FAGDDLPSTDISASVAMADK
2665 562.2994 1683.8764 1683.8832 -4.03 0 12 0.91 +1Score > 39 indicates identity
Score > 24 indicates homology
NITNQLITFLIAGHE + Deamidated (NQ)
535 352.2224 702.4302 702.4388 -12.2 1 12 0.29 +1Score > 38 indicates identity
Score > 19 indicates homology
ITKVSR
1495 573.8179 1145.6212 1145.6193 1.71 0 12 0.8 +1Score > 40 indicates identity
Score > 24 indicates homology
LDSHHPSILK
1529 387.8892 1160.6458 1160.6666 -17.9 1 12 0.62 +1Score > 39 indicates identity
Score > 23 indicates homology
FRQLNLITR + Deamidated (NQ)
1996 684.3632 1366.7118 1366.7092 1.93 1 12 0.58 +1Score > 40 indicates identity
Score > 22 indicates homology
LASKTDINYNTK
854 434.2055 866.3964 866.4021 -6.56 1 12 0.59 +1Score > 41 indicates identity
Score > 22 indicates homology
VENYVNK + 2 Deamidated (NQ)
1592 593.3483 1184.6820 1184.6990 -14.3 1 12 0.53 +1Score > 39 indicates identity
Score > 22 indicates homology
VAVASTGVIGRR
2155 482.9371 1445.7895 1445.7739 10.8 1 12 0.35 +1Score > 39 indicates identity
Score > 20 indicates homology
IQQINRDYLQR
2323 510.9542 1529.8408 1529.8678 -17.6 1 12 0.92 +1Score > 38 indicates identity
Score > 24 indicates homology
TYASLLPRNNLIR
1116 487.3133 972.6120 972.5968 15.7 1 12 0.7 +1Score > 30 indicates identity
Score > 23 indicates homology
ILKTGISNK
1314 356.8537 1067.5393 1067.5247 13.6 0 12 0.99 +1Score > 39 indicates identity
Score > 25 indicates homology
QVVSHSADPK + Deamidated (NQ)
1943 671.3810 1340.7474 1340.7551 -5.72 0 12 0.82 +1Score > 38 indicates identity
Score > 24 indicates homology
LNPSLVISLSQGL + Deamidated (NQ)
2448 793.4388 1584.8630 1584.8545 5.40 1 12 0.92 +1Score > 38 indicates identity
Score > 24 indicates homology
SMLSEPLLREPSVK
1580 591.3682 1180.7218 1180.7067 12.8 1 12 0.59 +1Score > 31 indicates identity
Score > 22 indicates homology
EIIQVLQLPK + Deamidated (NQ)
3444 725.3716 2173.0930 2173.1266 -15.5 1 12 0.71 +1Score > 39 indicates identity
Score > 23 indicates homology
TPGGTQSKVVPIASLNPYQSK + 2 Deamidated (NQ)
2138 718.3804 1434.7462 1434.7354 7.54 1 12 0.47 +1Score > 41 indicates identity
Score > 21 indicates homology
LQNQVLDSKNFK + 2 Deamidated (NQ)
2733 861.4271 1720.8396 1720.8342 3.19 0 12 0.88 +1Score > 41 indicates identity
Score > 24 indicates homology
IVMDVANGATFNLNPK + 2 Deamidated (NQ); Oxidation (M)
2678 847.4265 1692.8384 1692.8723 -20.0 1 12 0.86 +1Score > 41 indicates identity
Score > 24 indicates homology
GPPITEAYVTDVLYR
1662 608.3613 1214.7080 1214.6982 8.07 1 12 0.98 +1Score > 36 indicates identity
Score > 24 indicates homology
TIQNLSRIIR + 2 Deamidated (NQ)
537 352.6960 703.3774 703.3864 -12.8 0 12 0.9 +1Score > 44 indicates identity
Score > 24 indicates homology
QTGALSK
3155 667.0026 1997.9860 1997.9656 10.2 0 12 0.5 +1Score > 40 indicates identity
Score > 22 indicates homology
LDYLAPMSNNLGYVLAVE + Deamidated (NQ); Oxidation (M)
2802 588.6662 1762.9768 1762.9941 -9.85 0 12 0.18 +1Score > 35 indicates identity
Score > 17 indicates homology
VLGLGLPGNGTTLAIDPR
3066 644.3660 1930.0762 1930.0524 12.3 0 12 0.39 +1Score > 34 indicates identity
Score > 20 indicates homology
QSALVQVFNKPVQTITR + 2 Deamidated (NQ)
1848 432.9035 1295.6887 1295.6795 7.08 1 12 0.48 +1Score > 39 indicates identity
Score > 21 indicates homology
AMLAVLSEAFTK + Oxidation (M)
1505 385.5659 1153.6759 1153.6567 16.6 1 12 0.67 +1Score > 36 indicates identity
Score > 23 indicates homology
ITGINDPRLR
2866 601.3344 1800.9814 1800.9668 8.07 0 12 0.72 +1Score > 37 indicates identity
Score > 23 indicates homology
QGIHTPIIAMTAHALAR + Deamidated (NQ)
1172 334.1719 999.4939 999.5138 -19.9 1 12 0.6 +1Score > 40 indicates identity
Score > 22 indicates homology
GPPEVAFAGR
3110 657.0107 1968.0103 1968.0316 -10.9 1 12 0.16 +1Score > 39 indicates identity
Score > 17 indicates homology
ILQDIASGSHPFSQVLKE
1231 342.8497 1025.5273 1025.5254 1.83 0 12 0.5 +1Score > 39 indicates identity
Score > 21 indicates homology
EPATNGRPGK
3419 1078.0344 2154.0542 2154.0415 5.89 0 12 0.14 +1Score > 39 indicates identity
Score > 16 indicates homology
VSMAVLTYPDGNYGNLVDAR
1584 394.9005 1181.6797 1181.7019 -18.9 1 12 0.41 +1Score > 36 indicates identity
Score > 21 indicates homology
GAPKLQIDVLK + Deamidated (NQ)
3201 1012.5281 2023.0416 2023.0698 -13.9 1 12 0.19 +1Score > 39 indicates identity
Score > 17 indicates homology
SAALQSIQENLGLQDAPLR
2265 752.3928 1502.7710 1502.7801 -6.04 1 12 0.62 +1Score > 41 indicates identity
Score > 22 indicates homology
ITSRLSTTSHTSGR
2882 904.4610 1806.9074 1806.9220 -8.03 0 12 0.53 +1Score > 40 indicates identity
Score > 22 indicates homology
INIVVLGSMNTDLVMR + Deamidated (NQ); 2 Oxidation (M)
2990 939.4781 1876.9416 1876.9782 -19.5 1 12 0.45 +1Score > 40 indicates identity
Score > 21 indicates homology
YLNAVGSVLDLSIEDLR + Deamidated (NQ)
1799 637.8120 1273.6094 1273.5972 9.59 1 12 0.56 +1Score > 41 indicates identity
Score > 22 indicates homology
DHSGALSALMEK + Oxidation (M)
1846 431.9082 1292.7028 1292.7128 -7.79 1 12 0.64 +1Score > 39 indicates identity
Score > 22 indicates homology
QLPFDKYLLR + Deamidated (NQ)
1918 663.3430 1324.6714 1324.6551 12.3 1 12 0.97 +1Score > 40 indicates identity
Score > 24 indicates homology
VYPVVGTVYAEE
3204 1012.5317 2023.0488 2023.0837 -17.2 1 12 0.99 +1Score > 39 indicates identity
Score > 24 indicates homology
LSVIPDLLKGDVVANDNIE
544 354.6887 707.3628 707.3636 -1.07 0 12 0.39 +1Score > 43 indicates identity
Score > 20 indicates homology
MTTLAR + Oxidation (M)
1205 507.7922 1013.5698 1013.5645 5.32 1 12 0.26 +1Score > 41 indicates identity
Score > 18 indicates homology
DIIEIIGLE
3930 509.6463 2543.1951 2543.1883 2.67 1 12 0.35 +1Score > 38 indicates identity
Score > 20 indicates homology
LLNQMDGFDQTTNVKVIMATNR + 3 Deamidated (NQ); 2 Oxidation (M)
1195 337.5272 1009.5598 1009.5597 0.11 0 12 0.53 +1Score > 37 indicates identity
Score > 22 indicates homology
FFSLDILR
3415 1077.1179 2152.2212 2152.1786 19.8 1 12 0.34 +1Score > 31 indicates identity
Score > 20 indicates homology
RAVLLVSGGMDSLVATALAHR + Oxidation (M)
1589 593.3257 1184.6368 1184.6223 12.3 1 12 0.93 +1Score > 40 indicates identity
Score > 24 indicates homology
VDVNRMIPLE
3760 1190.6436 2379.2726 2379.2355 15.6 1 12 1 +1Score > 35 indicates identity
Score > 24 indicates homology
TNLGNFLSKINMNTIDIQITK + 2 Deamidated (NQ)
3931 509.6464 2543.1956 2543.1890 2.59 0 12 0.53 +1Score > 38 indicates identity
Score > 22 indicates homology
FDGQLWIDMDFVDGTDTVSLLR + Deamidated (NQ)
2117 712.3609 1422.7072 1422.7242 -11.9 1 12 0.8 +1Score > 41 indicates identity
Score > 23 indicates homology
ILGQSLSNAYVEK + 2 Deamidated (NQ)
3778 797.4395 2389.2967 2389.3104 -5.74 1 12 0.19 +1Score > 34 indicates identity
Score > 17 indicates homology
LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ)
1283 524.2662 1046.5178 1046.5244 -6.25 1 12 0.74 +1Score > 42 indicates identity
Score > 23 indicates homology
QTPASASVAKS + Deamidated (NQ)
232 489.2802 488.2729 488.2707 4.57 0 12 0.8 +1Score > 44 indicates identity
Score > 23 indicates homology
SGGIR
1573 589.2998 1176.5850 1176.6040 -16.1 1 12 0.94 +1Score > 42 indicates identity
Score > 24 indicates homology
RVGFGYDVHK
2025 693.8937 1385.7728 1385.7626 7.38 1 12 0.71 +1Score > 38 indicates identity
Score > 23 indicates homology
ILAANAATTRNIR + 2 Deamidated (NQ)
1329 358.5167 1072.5283 1072.5400 -11.0 0 12 0.7 +1Score > 41 indicates identity
Score > 23 indicates homology
DNDLIDVIR + Deamidated (NQ)
2620 833.4946 1664.9746 1665.0076 -19.8 0 12 0.094 +1Score > 31 indicates identity
Score > 14 indicates homology
SLPQAVLSAIVIVNLK + Deamidated (NQ)
1002 467.7308 933.4470 933.4477 -0.71 0 12 0.94 +1Score > 43 indicates identity
Score > 24 indicates homology
KPMDISNI + Deamidated (NQ); Oxidation (M)
2666 562.6516 1684.9330 1684.9182 8.79 1 12 0.51 +1Score > 37 indicates identity
Score > 21 indicates homology
LQKQMVPLQASAQVK + Deamidated (NQ); Oxidation (M)
3257 1030.5339 2059.0532 2059.0375 7.67 1 12 0.48 +1Score > 39 indicates identity
Score > 21 indicates homology
GDKVQGDGDWKPIVQFLR + 2 Deamidated (NQ)
3464 546.7999 2183.1705 2183.2062 -16.4 1 12 0.1 +1Score > 36 indicates identity
Score > 14 indicates homology
NYGPLPIGSNARSILLTLQR + Deamidated (NQ)
2737 574.9880 1721.9422 1721.9539 -6.79 1 12 0.54 +1Score > 37 indicates identity
Score > 22 indicates homology
QMDIGVFFGLKPKVK + Oxidation (M)
539 354.1788 706.3430 706.3432 -0.22 0 12 0.35 +1Score > 41 indicates identity
Score > 20 indicates homology
QMSGIR + Oxidation (M)
2680 565.6216 1693.8430 1693.8523 -5.49 0 12 0.5 +1Score > 41 indicates identity
Score > 21 indicates homology
AVLGSNHVQLQLAVDE + 2 Deamidated (NQ)
2244 499.3066 1494.8980 1494.8704 18.4 1 12 0.68 +1Score > 30 indicates identity
Score > 23 indicates homology
RMEPAALLNVIIR
1348 542.7627 1083.5108 1083.5309 -18.5 0 12 0.31 +1Score > 40 indicates identity
Score > 19 indicates homology
IGGSSGGHNGLK + Deamidated (NQ)
596 368.7281 735.4416 735.4391 3.40 0 12 0.85 +1Score > 34 indicates identity
Score > 23 indicates homology
LPHTIR
2679 424.2240 1692.8669 1692.8656 0.78 1 12 0.63 +1Score > 40 indicates identity
Score > 22 indicates homology
TASTPHTTRPEPSRR
981 464.2743 926.5340 926.5297 4.65 1 12 0.63 +1Score > 38 indicates identity
Score > 22 indicates homology
RLGTNPLR + Deamidated (NQ)
1153 497.8385 993.6624 993.6586 3.83 0 12 0.66 +1Score > 24 indicates identity
Score > 22 indicates homology
KPLVIVTPK
1100 485.7663 969.5180 969.5284 -10.6 0 12 0.95 +1Score > 39 indicates identity
Score > 24 indicates homology
DVPNVWIK
2855 598.9818 1793.9236 1793.9496 -14.5 1 12 0.77 +1Score > 39 indicates identity
Score > 23 indicates homology
AARATLNQDITLHSAGR
2338 514.2926 1539.8560 1539.8257 19.7 0 12 0.93 +1Score > 37 indicates identity
Score > 24 indicates homology
GQLQQIVDIQLQR + 2 Deamidated (NQ)
4003 659.8129 2635.2225 2635.2186 1.47 1 12 0.16 +1Score > 37 indicates identity
Score > 16 indicates homology
GQTVFMGDFPLMTDKGTFIINGTE + Deamidated (NQ); Oxidation (M)
3134 663.3200 1986.9382 1986.9534 -7.67 1 12 0.42 +1Score > 39 indicates identity
Score > 20 indicates homology
FSHINQPVKTLSGGNQQK + 5 Deamidated (NQ)
399 321.2036 640.3926 640.3908 2.87 0 12 0.48 +1Score > 35 indicates identity
Score > 21 indicates homology
QPVAVK
3634 763.4363 2287.2871 2287.2827 1.89 0 12 0.36 +1Score > 31 indicates identity
Score > 20 indicates homology
VLFLQGGASLQFSAIPLNLLGK + 2 Deamidated (NQ)
2879 602.3057 1803.8953 1803.9077 -6.87 1 12 0.58 +1Score > 40 indicates identity
Score > 22 indicates homology
ALDFVTMPAPQLASAEK + Oxidation (M)
477 341.6983 681.3820 681.3810 1.59 1 12 0.99 +1Score > 37 indicates identity
Score > 24 indicates homology
AHVVEK
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