MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 3754)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
747 411.2446 820.4746 820.4694 6.35 0 13 1 +1Score > 37 indicates identity
Score > 26 indicates homology
LNSFVIK + Deamidated (NQ)
1374 548.3366 1094.6586 1094.6560 2.43 1 13 0.48 +1Score > 32 indicates identity
Score > 23 indicates homology
ARVALPQALR + Deamidated (NQ)
1812 427.2394 1278.6964 1278.6932 2.50 1 13 0.63 +1Score > 38 indicates identity
Score > 24 indicates homology
QEIIPADIQPR
3160 1001.0354 2000.0562 2000.0765 -10.1 1 13 0.8 +1Score > 38 indicates identity
Score > 25 indicates homology
LVITPQDAEMVVGFLVNR
980 464.2740 926.5334 926.5185 16.1 0 13 0.77 +1Score > 38 indicates identity
Score > 25 indicates homology
VLQNSLPR + Deamidated (NQ)
3173 1005.9935 2009.9724 2009.9483 12.0 0 13 0.69 +1Score > 40 indicates identity
Score > 24 indicates homology
VYIGSFWAQPLQNTDNR + 2 Deamidated (NQ)
3239 682.6946 2045.0620 2045.0629 -0.44 1 13 0.5 +1Score > 38 indicates identity
Score > 23 indicates homology
MAKAHDLIAWNAPHSLVR + Oxidation (M)
940 455.7567 909.4988 909.4920 7.56 1 13 0.59 +1Score > 38 indicates identity
Score > 23 indicates homology
GPVNPKGLE
2204 738.4019 1474.7892 1474.7892 0.0014 1 13 0.72 +1Score > 40 indicates identity
Score > 24 indicates homology
LSVRGFVDSVGPSR
3370 1068.5616 2135.1086 2135.0858 10.7 1 13 0.57 +1Score > 38 indicates identity
Score > 23 indicates homology
ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
2389 781.9593 1561.9040 1561.9007 2.15 0 13 0.95 +1Score > 32 indicates identity
Score > 26 indicates homology
IFLFLAILLQQLE + 2 Deamidated (NQ)
3159 667.0266 1998.0580 1998.0720 -7.04 1 13 0.26 +1Score > 38 indicates identity
Score > 20 indicates homology
MDIKLINIGFGNIVSAHR + Deamidated (NQ)
1034 316.1812 945.5218 945.5131 9.18 0 13 0.85 +1Score > 41 indicates identity
Score > 25 indicates homology
QATLSAQVK + Deamidated (NQ)
1772 421.2321 1260.6745 1260.6714 2.45 1 13 0.87 +1Score > 41 indicates identity
Score > 25 indicates homology
VGYSVPQELLR + Deamidated (NQ)
551 357.7006 713.3866 713.3959 -13.0 0 13 0.37 +1Score > 38 indicates identity
Score > 21 indicates homology
VQQLPK + 2 Deamidated (NQ)
1204 507.7913 1013.5680 1013.5757 -7.55 1 13 0.73 +1Score > 41 indicates identity
Score > 24 indicates homology
KDVQLIAVE
776 418.2253 834.4360 834.4348 1.51 0 13 0.89 +1Score > 42 indicates identity
Score > 25 indicates homology
VAVNYNR
2449 529.6353 1585.8841 1585.8610 14.5 1 13 0.41 +1Score > 37 indicates identity
Score > 22 indicates homology
IDGMDVRGLTLAGLR
3248 685.6808 2054.0206 2053.9853 17.2 0 13 0.12 +1Score > 39 indicates identity
Score > 16 indicates homology
ISSPFLQMAQDPTMPTYK
1591 593.3320 1184.6494 1184.6401 7.91 1 13 0.9 +1Score > 40 indicates identity
Score > 25 indicates homology
QLQEVLDIAR + Deamidated (NQ)
973 308.5166 922.5280 922.5123 16.9 1 13 0.54 +1Score > 37 indicates identity
Score > 23 indicates homology
KYNLSVAK + Deamidated (NQ)
2087 705.9500 1409.8854 1409.8606 17.6 1 13 0.9 +1Score > 25 indicates identity KLLPATVVNQVTK
2430 527.3039 1578.8899 1578.9205 -19.4 0 13 0.71 +1Score > 35 indicates identity
Score > 24 indicates homology
AAQQLLAQRPTILR + Deamidated (NQ)
2760 866.8901 1731.7656 1731.7807 -8.72 0 13 0.33 +1Score > 39 indicates identity
Score > 21 indicates homology
NDNPLVDMQAMSPTAK + Deamidated (NQ)
3750 790.0566 2367.1480 2367.1239 10.2 0 13 0.29 +1Score > 39 indicates identity
Score > 20 indicates homology
ILLMANGAQYPCDQVGVFTPR + 2 Deamidated (NQ); Oxidation (M)
1931 445.2645 1332.7717 1332.7613 7.79 1 13 0.29 +1Score > 34 indicates identity
Score > 20 indicates homology
VVALTGSSGKTSVK
2842 597.3100 1788.9082 1788.8979 5.73 1 13 0.3 +1Score > 40 indicates identity
Score > 20 indicates homology
RPVTGNNVSHAHNKTR + 2 Deamidated (NQ)
2181 488.2868 1461.8386 1461.8265 8.28 1 13 0.81 +1Score > 34 indicates identity
Score > 25 indicates homology
MKFIINNNILIK + 2 Deamidated (NQ)
2649 836.9432 1671.8718 1671.8792 -4.37 1 13 0.45 +1Score > 40 indicates identity
Score > 22 indicates homology
NRAQVVIDTTTLSPR + 2 Deamidated (NQ)
480 342.2272 682.4398 682.4378 3.06 0 13 0.33 +1Score > 34 indicates identity
Score > 21 indicates homology
VPAVIGK
773 417.2207 832.4268 832.4153 13.9 1 13 0.97 +1Score > 43 indicates identity
Score > 25 indicates homology
KYTFMK + Oxidation (M)
2109 474.2591 1419.7555 1419.7432 8.66 0 13 0.66 +1Score > 40 indicates identity
Score > 24 indicates homology
GIMLQTLDSLWK + Oxidation (M)
1605 595.8265 1189.6384 1189.6376 0.68 1 13 0.39 +1Score > 40 indicates identity
Score > 22 indicates homology
QIQMVKQIGK + 2 Deamidated (NQ); Oxidation (M)
2856 897.9706 1793.9266 1793.9047 12.2 1 13 0.59 +1Score > 39 indicates identity
Score > 23 indicates homology
SSPGTLSLVVPSAPPQEE
1247 345.5028 1033.4866 1033.5040 -16.8 1 13 0.98 +1Score > 41 indicates identity
Score > 25 indicates homology
KQSQQANTK + 2 Deamidated (NQ)
629 380.2540 758.4934 758.4902 4.32 0 13 0.96 +1Score > 30 indicates identity
Score > 25 indicates homology
VLISSLK
1895 439.8852 1316.6338 1316.6217 9.19 0 13 0.32 +1Score > 41 indicates identity
Score > 21 indicates homology
DLLGTNMMLHR + Deamidated (NQ); Oxidation (M)
2644 835.5045 1668.9944 1668.9661 17.0 1 13 0.18 +1Score > 29 indicates identity
Score > 18 indicates homology
LNIILQGNIILSTEK + Deamidated (NQ)
2898 907.9396 1813.8646 1813.8992 -19.1 1 13 0.69 +1Score > 40 indicates identity
Score > 24 indicates homology
SHEPKSPSLGMISTATR + Oxidation (M)
3043 959.0071 1915.9996 1915.9673 16.9 1 13 0.23 +1Score > 39 indicates identity
Score > 19 indicates homology
VSIGSQELQIVCGANNVK + Deamidated (NQ)
4062 901.8058 2702.3956 2702.3731 8.32 1 13 0.12 +1Score > 36 indicates identity
Score > 16 indicates homology
AVQFIDLLQKVYNDFGFNDILVK + 4 Deamidated (NQ)
3237 682.6933 2045.0581 2045.0847 -13.0 1 13 0.93 +1Score > 39 indicates identity
Score > 25 indicates homology
LADREPWAPLLHDIPFR
3290 696.6765 2087.0077 2087.0292 -10.3 1 13 0.4 +1Score > 39 indicates identity
Score > 21 indicates homology
ALAAEPSPAMLHPQCELPR
2377 779.8904 1557.7662 1557.7900 -15.2 1 13 0.7 +1Score > 41 indicates identity
Score > 24 indicates homology
AVDAGPIAFVRDGDR
1063 319.8389 956.4949 956.4927 2.26 0 13 0.92 +1Score > 39 indicates identity
Score > 25 indicates homology
VVSDPNGIR + Deamidated (NQ)
3007 629.6716 1885.9930 1885.9971 -2.21 0 13 0.96 +1Score > 39 indicates identity
Score > 25 indicates homology
ASLMFDHVILAIAASPSK + Oxidation (M)
2452 794.4112 1586.8078 1586.8093 -0.91 1 13 0.29 +1Score > 40 indicates identity
Score > 20 indicates homology
LKGFWGQDVAPIQE
3664 768.3841 2302.1305 2302.1176 5.58 1 13 0.74 +1Score > 39 indicates identity
Score > 24 indicates homology
SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
1957 450.5668 1348.6786 1348.6908 -9.07 0 13 0.95 +1Score > 41 indicates identity
Score > 25 indicates homology
LVGVQSNSMVLGK + 2 Deamidated (NQ); Oxidation (M)
1537 389.5573 1165.6501 1165.6417 7.21 0 13 0.48 +1Score > 37 indicates identity
Score > 22 indicates homology
SFLMQILGLK + Deamidated (NQ); Oxidation (M)
3811 605.3210 2417.2549 2417.2660 -4.58 0 13 0.19 +1Score > 37 indicates identity
Score > 18 indicates homology
LSAMMVTSFILISVLALGLMYE + Oxidation (M)
2693 851.4203 1700.8260 1700.8370 -6.42 0 13 0.15 +1Score > 40 indicates identity
Score > 17 indicates homology
LGIDFQSDAQHVTIR + 2 Deamidated (NQ)
2933 613.6424 1837.9054 1837.9349 -16.1 1 13 0.69 +1Score > 40 indicates identity
Score > 24 indicates homology
YNVEIVLPVDYVTADK + Deamidated (NQ)
576 364.7024 727.3902 727.3864 5.23 0 13 0.49 +1Score > 38 indicates identity
Score > 22 indicates homology
QADPAVK
989 310.1635 927.4687 927.4848 -17.4 0 13 0.8 +1Score > 40 indicates identity
Score > 24 indicates homology
MADLPPLR + Oxidation (M)
1565 391.8609 1172.5609 1172.5648 -3.37 1 13 0.94 +1Score > 40 indicates identity
Score > 25 indicates homology
HFVEMEPIR + Oxidation (M)
2493 804.8950 1607.7754 1607.7732 1.37 1 13 0.66 +1Score > 41 indicates identity
Score > 24 indicates homology
LGWAYQGPEQGFVR + Deamidated (NQ)
2168 729.8618 1457.7090 1457.6859 15.9 1 13 0.54 +1Score > 40 indicates identity
Score > 23 indicates homology
ISQGNEQAAQQQR + Deamidated (NQ)
2552 814.9684 1627.9222 1627.9144 4.80 1 13 0.82 +1Score > 35 indicates identity
Score > 25 indicates homology
LGINASIGIANSKVAAK + 2 Deamidated (NQ)
570 364.2178 726.4210 726.4177 4.62 0 13 0.95 +1Score > 36 indicates identity
Score > 25 indicates homology
GPVWLR
2459 531.2817 1590.8233 1590.8478 -15.4 0 13 0.66 +1Score > 40 indicates identity
Score > 24 indicates homology
ISQGNHLPQSILQR + Deamidated (NQ)
530 351.7118 701.4090 701.3959 18.7 0 13 0.78 +1Score > 43 indicates identity
Score > 24 indicates homology
IIDNVK + Deamidated (NQ)
1461 567.3404 1132.6662 1132.6492 15.1 1 13 0.36 +1Score > 35 indicates identity
Score > 21 indicates homology
LFLSGINKIE
3694 772.7196 2315.1370 2315.1065 13.2 1 13 0.77 +1Score > 39 indicates identity
Score > 24 indicates homology
MNNYETVFILTPVLSDAQMK + 2 Deamidated (NQ)
237 501.3168 500.3095 500.3071 4.88 0 13 0.18 +1Score > 43 indicates identity
Score > 18 indicates homology
AVVGR
2483 803.9312 1605.8478 1605.8402 4.75 1 13 0.44 +1Score > 39 indicates identity
Score > 22 indicates homology
ALSLKSNQALWFVE + Deamidated (NQ)
2817 886.9542 1771.8938 1771.8815 6.99 1 13 0.23 +1Score > 40 indicates identity
Score > 19 indicates homology
VNFSVNFKCLSSQIK + 2 Deamidated (NQ)
1352 362.5461 1084.6165 1084.6142 2.14 1 13 0.52 +1Score > 40 indicates identity
Score > 22 indicates homology
QGAKGLAWVR
1092 322.5317 964.5733 964.5593 14.5 0 13 0.94 +1Score > 34 indicates identity
Score > 25 indicates homology
SSLFIIASK
2061 701.3842 1400.7538 1400.7623 -6.05 1 13 0.27 +1Score > 40 indicates identity
Score > 20 indicates homology
SVTQVNRDLLAGK + Deamidated (NQ)
829 428.7311 855.4476 855.4450 3.07 0 13 0.71 +1Score > 39 indicates identity
Score > 24 indicates homology
LADPDVAR
1406 557.3237 1112.6328 1112.6302 2.39 1 13 0.38 +1Score > 38 indicates identity
Score > 21 indicates homology
SIVPISRGQR + Deamidated (NQ)
463 337.2183 672.4220 672.4170 7.47 0 13 0.84 +1Score > 40 indicates identity
Score > 24 indicates homology
AVSVAVK
1794 636.8410 1271.6674 1271.6656 1.46 1 13 0.34 +1Score > 41 indicates identity
Score > 21 indicates homology
MRIAIDGPAGAGK + Oxidation (M)
3596 564.5308 2254.0941 2254.1351 -18.2 1 13 0.82 +1Score > 39 indicates identity
Score > 24 indicates homology
ASMGHQPMLVLSADRPFELR
595 368.2256 734.4366 734.4327 5.41 0 13 0.59 +1Score > 36 indicates identity
Score > 23 indicates homology
LVFQTK
2040 464.2604 1389.7594 1389.7537 4.06 0 13 0.32 +1Score > 39 indicates identity
Score > 20 indicates homology
QAIQMVLLATASK + Deamidated (NQ); Oxidation (M)
748 411.2451 820.4756 820.4807 -6.14 0 13 0.95 +1Score > 37 indicates identity
Score > 25 indicates homology
IPHDLVK
1142 495.2822 988.5498 988.5627 -13.0 1 13 0.71 +1Score > 40 indicates identity
Score > 24 indicates homology
METALLLAK
2698 568.6266 1702.8580 1702.8485 5.54 1 13 0.34 +1Score > 40 indicates identity
Score > 20 indicates homology
DAAASGRNNLVSQLASK + 2 Deamidated (NQ)
1500 384.1908 1149.5506 1149.5410 8.37 1 13 0.55 +1Score > 42 indicates identity
Score > 23 indicates homology
KSGMIPLQME + Deamidated (NQ); Oxidation (M)
1971 677.8814 1353.7482 1353.7728 -18.2 1 13 0.21 +1Score > 38 indicates identity
Score > 18 indicates homology
IVRSILDGATGPR
2841 895.4600 1788.9054 1788.9298 -13.6 0 13 0.65 +1Score > 40 indicates identity
Score > 23 indicates homology
ISFDLNVAQGLALLWE + Deamidated (NQ)
2984 938.4536 1874.8926 1874.9084 -8.40 1 13 0.94 +1Score > 40 indicates identity
Score > 25 indicates homology
SVPCNINIFGDPKQGVK + 3 Deamidated (NQ)
2240 499.2213 1494.6421 1494.6297 8.31 1 13 0.29 +1Score > 38 indicates identity
Score > 20 indicates homology
AEPTEQSFMQPGR + 2 Deamidated (NQ); Oxidation (M)
1604 397.5410 1189.6012 1189.6091 -6.68 1 13 0.74 +1Score > 42 indicates identity
Score > 24 indicates homology
QFLRNQGTPK + 2 Deamidated (NQ)
1526 580.3619 1158.7092 1158.7046 4.02 1 13 0.091 +1Score > 31 indicates identity
Score > 15 indicates homology
SLPLLMITKK + Oxidation (M)
2019 692.8253 1383.6360 1383.6227 9.62 1 13 0.27 +1Score > 40 indicates identity
Score > 19 indicates homology
NAINENTAFLMK + 3 Deamidated (NQ); Oxidation (M)
2081 470.5646 1408.6720 1408.6616 7.35 1 13 0.44 +1Score > 41 indicates identity
Score > 21 indicates homology
SASGDNMSKIIDR + Oxidation (M)
3749 789.4321 2365.2745 2365.2893 -6.26 0 13 0.12 +1Score > 35 indicates identity
Score > 16 indicates homology
NILDINTGVQEPPRPLFSLIK + 2 Deamidated (NQ)
1485 381.5583 1141.6531 1141.6316 18.8 1 13 0.73 +1Score > 39 indicates identity
Score > 24 indicates homology
AARALTANAQR
3021 633.3229 1896.9469 1896.9403 3.45 0 13 0.71 +1Score > 40 indicates identity
Score > 24 indicates homology
IMQQNIAILYQQYNR + 2 Deamidated (NQ)
3673 1154.0577 2306.1008 2306.0962 2.00 1 13 0.76 +1Score > 39 indicates identity
Score > 24 indicates homology
MNNLIKAYAAGVMSAAFLFGSE + 2 Deamidated (NQ)
2372 519.3008 1554.8806 1554.8729 4.91 1 12 0.8 +1Score > 36 indicates identity
Score > 24 indicates homology
VAAISTIVNRGQTPK + Deamidated (NQ)
1623 600.3375 1198.6604 1198.6418 15.5 1 12 0.66 +1Score > 40 indicates identity
Score > 23 indicates homology
DVLALARDGNR
1862 651.3877 1300.7608 1300.7755 -11.2 1 12 0.54 +1Score > 35 indicates identity
Score > 22 indicates homology
LQPYVLKVVNK + Deamidated (NQ)
2900 605.9786 1814.9140 1814.9149 -0.52 1 12 0.65 +1Score > 40 indicates identity
Score > 23 indicates homology
SQLLISAEPIVVSSDEE
1540 583.8336 1165.6526 1165.6342 15.8 1 12 0.66 +1Score > 37 indicates identity
Score > 23 indicates homology
NAIGIELPNPK + Deamidated (NQ)
2659 558.6533 1672.9381 1672.9294 5.18 1 12 0.26 +1Score > 35 indicates identity
Score > 19 indicates homology
VSLQNLSMVLRQLR + Deamidated (NQ); Oxidation (M)
2735 574.6246 1720.8520 1720.8414 6.16 1 12 0.61 +1Score > 41 indicates identity
Score > 23 indicates homology
SSTEIGANSVSMAAAAVR
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