MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2996 628.0028 1880.9866 1880.9567 15.9 1 14 0.62 +1Score > 38 indicates identity
Score > 25 indicates homology		 IEALTGWNAMHAVLAQR + Deamidated (NQ)
600 372.1891 742.3636 742.3610 3.62 1 14 0.22 +1Score > 38 indicates identity
Score > 20 indicates homology		 GPEDGLR
302 586.3882 585.3809 585.3737 12.3 0 14 0.09 +1Score > 39 indicates identity
Score > 16 indicates homology		 ILDIL
1342 541.3409 1080.6672 1080.6655 1.63 1 14 0.45 +1Score > 31 indicates identity
Score > 23 indicates homology		 AQLKLAALPR + Deamidated (NQ)
1658 304.6740 1214.6669 1214.6619 4.14 1 14 0.59 +1Score > 40 indicates identity
Score > 24 indicates homology		 VASLNKQIGQR + 2 Deamidated (NQ)
296 574.3347 573.3274 573.3234 6.95 0 14 0.68 +1Score > 42 indicates identity
Score > 25 indicates homology		 AGASIR
376 628.3802 627.3729 627.3704 4.06 1 14 0.96 +1Score > 37 indicates identity
Score > 26 indicates homology		 AAPNKK
792 842.5145 841.5072 841.5021 6.05 1 14 0.51 +1Score > 40 indicates identity
Score > 24 indicates homology		 KDITIPR
3321 704.3729 2110.0969 2110.0921 2.26 1 14 0.28 +1Score > 38 indicates identity
Score > 21 indicates homology		 LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
3358 1064.5641 2127.1136 2127.1358 -10.4 1 14 0.49 +1Score > 38 indicates identity
Score > 24 indicates homology		 SSPGGLLNMVSKRPTTEPLK + Oxidation (M)
486 343.7149 685.4152 685.4235 -12.0 1 14 0.44 +1Score > 41 indicates identity
Score > 23 indicates homology		 NAVVKR
2357 387.9576 1547.8013 1547.8056 -2.75 1 14 0.63 +1Score > 40 indicates identity
Score > 25 indicates homology		 LDRNLHNQPALVR + 3 Deamidated (NQ)
2190 735.3737 1468.7328 1468.7521 -13.1 0 14 0.67 +1Score > 40 indicates identity
Score > 25 indicates homology		 QIQAVPIANSQAAR + 3 Deamidated (NQ)
813 425.7631 849.5116 849.5072 5.20 0 14 0.43 +1Score > 36 indicates identity
Score > 23 indicates homology		 GPIPLPTR
815 426.2128 850.4110 850.4185 -8.71 1 14 0.87 +1Score > 42 indicates identity
Score > 26 indicates homology		 IEQFASR + Deamidated (NQ)
1901 660.3888 1318.7630 1318.7497 10.1 1 14 0.77 +1Score > 34 indicates identity
Score > 25 indicates homology		 FLGTTKDPVTLK
3076 646.7035 1937.0887 1937.0768 6.11 0 14 0.29 +1Score > 33 indicates identity
Score > 21 indicates homology		 DAIIVGCAQVLALVPGVSR
1822 427.5953 1279.7641 1279.7864 -17.4 0 14 0.81 +1Score > 33 indicates identity
Score > 26 indicates homology		 GLSLLVPVQLNK
3254 1030.5322 2059.0498 2059.0545 -2.27 1 14 0.74 +1Score > 39 indicates identity
Score > 25 indicates homology		 IVSSQINSGNALSADVQKAR + 2 Deamidated (NQ)
1781 422.9205 1265.7397 1265.7231 13.1 1 14 0.27 +1Score > 34 indicates identity
Score > 21 indicates homology		 LPISLPNGVEVK + Deamidated (NQ)
3891 835.0854 2502.2344 2502.2446 -4.09 1 14 0.11 +1Score > 38 indicates identity
Score > 17 indicates homology		 TAQMGWSLARLIAQMGWGAPAMR
475 340.7041 679.3936 679.3904 4.72 1 14 0.78 +1Score > 35 indicates identity
Score > 25 indicates homology		 YKQIK + Deamidated (NQ)
682 783.4683 782.4610 782.4538 9.26 0 14 0.25 +1Score > 36 indicates identity
Score > 20 indicates homology		 QAIPLLE
2616 555.2959 1662.8659 1662.8537 7.35 1 14 0.2 +1Score > 40 indicates identity
Score > 19 indicates homology		 ISGASGLASNTTNTKNK
715 400.7723 799.5300 799.5167 16.7 1 14 1 +1Score > 36 indicates identity
Score > 26 indicates homology		 KVQAIIK + Deamidated (NQ)
2871 901.9120 1801.8094 1801.8094 0.051 1 14 0.81 +1Score > 39 indicates identity
Score > 26 indicates homology		 NGSSAKWMGPFYIGDR + Deamidated (NQ); Oxidation (M)
1340 540.3041 1078.5936 1078.5883 4.96 1 14 0.43 +1Score > 39 indicates identity
Score > 23 indicates homology		 IISNRASYR
294 572.3551 571.3478 571.3442 6.36 0 14 0.48 +1Score > 42 indicates identity
Score > 23 indicates homology		 AGAVVR
470 675.3818 674.3745 674.3711 5.04 1 14 0.72 +1Score > 40 indicates identity
Score > 25 indicates homology		 KISDGR
2833 890.4459 1778.8772 1778.8985 -11.9 1 14 0.43 +1Score > 41 indicates identity
Score > 23 indicates homology		 NVASAQGPTGLGKYLMR + Deamidated (NQ); Oxidation (M)
705 398.1996 794.3846 794.3922 -9.56 0 14 0.56 +1Score > 41 indicates identity
Score > 24 indicates homology		 LHQPGNK + 2 Deamidated (NQ)
1257 518.2981 1034.5816 1034.5913 -9.34 0 14 0.68 +1Score > 38 indicates identity
Score > 25 indicates homology		 LFGPLSFVR
2934 920.0056 1837.9966 1837.9897 3.76 1 14 0.12 +1Score > 37 indicates identity
Score > 17 indicates homology		 AQLVAEALSPGANVSAIAR + Deamidated (NQ)
2995 627.6750 1880.0032 1880.0115 -4.45 1 14 0.74 +1Score > 38 indicates identity
Score > 25 indicates homology		 AQAAQPAAKGAGSKPQGLTK + Deamidated (NQ)
1080 480.7741 959.5336 959.5287 5.12 1 14 0.93 +1Score > 41 indicates identity
Score > 26 indicates homology		 LGNNVKVSK + 2 Deamidated (NQ)
2491 402.7379 1606.9225 1606.8977 15.4 1 14 0.31 +1Score > 33 indicates identity
Score > 21 indicates homology		 KLQNLIHIMNNIR + Deamidated (NQ)
516 351.2041 700.3936 700.3868 9.81 0 14 0.34 +1Score > 42 indicates identity
Score > 22 indicates homology		 VAQQAGK
561 361.2171 720.4196 720.4170 3.64 0 14 0.72 +1Score > 38 indicates identity
Score > 25 indicates homology		 ASLGFVK
1478 380.5695 1138.6867 1138.6710 13.8 0 14 0.9 +1Score > 33 indicates identity
Score > 26 indicates homology		 VSILPGTPLSR
373 314.6760 627.3374 627.3340 5.47 0 14 0.54 +1Score > 38 indicates identity
Score > 24 indicates homology		 IGADPR
219 474.2707 473.2634 473.2598 7.70 0 14 0.33 +1Score > 47 indicates identity
Score > 21 indicates homology		 AGAAGK
2073 703.8940 1405.7734 1405.7790 -3.94 0 14 0.71 +1Score > 38 indicates identity
Score > 25 indicates homology		 PAARPAGAGVGLVDR
722 401.2505 800.4864 800.4909 -5.51 0 14 0.84 +1Score > 40 indicates identity
Score > 26 indicates homology		 GIVLPFR
1651 405.2052 1212.5938 1212.6040 -8.41 1 14 0.47 +1Score > 40 indicates identity
Score > 23 indicates homology		 WFYNNVKSR
3133 663.3196 1986.9370 1986.9534 -8.28 1 14 0.39 +1Score > 39 indicates identity
Score > 22 indicates homology		 FSHINQPVKTLSGGNQQK + 5 Deamidated (NQ)
1477 570.2638 1138.5130 1138.5117 1.15 1 14 0.28 +1Score > 40 indicates identity
Score > 21 indicates homology		 FSAMGVWNEV
1311 534.2437 1066.4728 1066.4713 1.47 1 14 0.88 +1Score > 40 indicates identity
Score > 26 indicates homology		 AMKSASDNAR + Deamidated (NQ); Oxidation (M)
1700 410.2289 1227.6649 1227.6823 -14.2 0 14 0.8 +1Score > 40 indicates identity
Score > 25 indicates homology		 DQVIGVALQASK
2752 576.9918 1727.9536 1727.9206 19.1 1 14 0.27 +1Score > 37 indicates identity
Score > 20 indicates homology		 FNTLHQLLRTLQDK + 2 Deamidated (NQ)
1423 560.8060 1119.5974 1119.5924 4.50 0 14 0.98 +1Score > 41 indicates identity
Score > 26 indicates homology		 GAVVAYANDLK
262 529.3475 528.3402 528.3384 3.52 0 14 0.95 +1Score > 41 indicates identity
Score > 26 indicates homology		 IIGAR
1395 554.2526 1106.4906 1106.4914 -0.65 1 14 0.78 +1Score > 40 indicates identity
Score > 25 indicates homology		 QCVETNNLK + 2 Deamidated (NQ)
1442 564.8273 1127.6400 1127.6298 9.07 0 14 0.4 +1Score > 40 indicates identity
Score > 22 indicates homology		 LLNLSLANNR + Deamidated (NQ)
581 365.2258 728.4370 728.4293 10.6 1 14 0.9 +1Score > 41 indicates identity
Score > 26 indicates homology		 ALAGKNR
1307 531.7688 1061.5230 1061.5288 -5.38 1 14 0.55 +1Score > 42 indicates identity
Score > 24 indicates homology		 IRDMADGIR + Oxidation (M)
3334 1059.0403 2116.0660 2116.0973 -14.8 0 14 0.67 +1Score > 39 indicates identity
Score > 24 indicates homology		 TVTVLNTILLQNMGAALTPE + 2 Deamidated (NQ); Oxidation (M)
2508 538.2925 1611.8557 1611.8580 -1.45 1 14 0.57 +1Score > 39 indicates identity
Score > 24 indicates homology		 LSARPGNEVNLLDSK
1262 519.2742 1036.5338 1036.5189 14.4 0 14 0.92 +1Score > 40 indicates identity
Score > 26 indicates homology		 IAQVSQYAR + 2 Deamidated (NQ)
3014 631.6670 1891.9792 1892.0043 -13.3 1 14 0.88 +1Score > 39 indicates identity
Score > 26 indicates homology		 EPPAHVKFLLATTDPQK + Deamidated (NQ)
939 455.7202 909.4258 909.4300 -4.52 0 14 0.73 +1Score > 41 indicates identity
Score > 25 indicates homology		 MLMDSVAK + Oxidation (M)
1473 380.2027 1137.5863 1137.5700 14.3 1 14 0.4 +1Score > 41 indicates identity
Score > 22 indicates homology		 GKSASDMVTVK + Oxidation (M)
1747 623.3163 1244.6180 1244.6361 -14.5 1 14 0.55 +1Score > 41 indicates identity
Score > 23 indicates homology		 QTQNAEGGIISK
1839 647.2911 1292.5676 1292.5593 6.49 0 14 0.65 +1Score > 39 indicates identity
Score > 24 indicates homology		 GAGSNAGASQSTQR + 2 Deamidated (NQ)
3174 1005.9955 2009.9764 2009.9483 14.0 0 14 0.91 +1Score > 40 indicates identity
Score > 26 indicates homology		 VYIGSFWAQPLQNTDNR + 2 Deamidated (NQ)
642 382.2097 762.4048 762.4163 -15.1 1 14 0.87 +1Score > 43 indicates identity
Score > 25 indicates homology		 ILFEIE
741 408.2357 814.4568 814.4548 2.45 1 14 0.93 +1Score > 43 indicates identity
Score > 26 indicates homology		 EIIIDGR
1628 400.8657 1199.5753 1199.5935 -15.2 1 14 0.89 +1Score > 40 indicates identity
Score > 26 indicates homology		 KFDFSNLSSR
387 316.1874 630.3602 630.3701 -15.6 0 14 0.44 +1Score > 44 indicates identity
Score > 22 indicates homology		 NVGTLK
303 586.3886 585.3813 585.3737 12.9 0 14 0.41 +1Score > 39 indicates identity
Score > 22 indicates homology		 ILDIL
2661 559.2846 1674.8320 1674.8134 11.1 1 14 0.74 +1Score > 41 indicates identity
Score > 25 indicates homology		 GENIINIITGPNMSGK + 2 Deamidated (NQ); Oxidation (M)
3077 969.5537 1937.0928 1937.0595 17.2 1 13 0.71 +1Score > 33 indicates identity
Score > 24 indicates homology		 RAVAGNLSNILFIHADAR
1012 470.3020 938.5894 938.5913 -1.95 0 13 0.98 +1Score > 32 indicates identity
Score > 26 indicates homology		 LLAPAVLSR
1739 413.9052 1238.6938 1238.6731 16.7 1 13 0.82 +1Score > 38 indicates identity
Score > 25 indicates homology		 QRVAADLPVDR
2001 457.9180 1370.7322 1370.7154 12.2 1 13 0.45 +1Score > 40 indicates identity
Score > 23 indicates homology		 LGQLDQGEGSVLR
1560 391.5692 1171.6858 1171.6673 15.8 1 13 0.59 +1Score > 37 indicates identity
Score > 24 indicates homology		 QLQTGLSRLR + Deamidated (NQ)
2038 695.8790 1389.7434 1389.7616 -13.1 0 13 0.92 +1Score > 40 indicates identity
Score > 26 indicates homology		 YLGIPLSSATVNR
3253 1030.5316 2059.0486 2059.0884 -19.3 1 13 0.85 +1Score > 39 indicates identity
Score > 25 indicates homology		 ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
3665 768.3849 2302.1329 2302.1176 6.63 1 13 0.96 +1Score > 39 indicates identity
Score > 26 indicates homology		 SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
3062 965.5446 1929.0746 1929.0459 14.9 0 13 0.57 +1Score > 34 indicates identity
Score > 24 indicates homology		 FLTLGGNGIISVANNIVPK + 3 Deamidated (NQ)
3191 673.7212 2018.1418 2018.1459 -2.03 1 13 0.094 +1Score > 32 indicates identity
Score > 16 indicates homology		 DLLVRLDLIAMAPGRPPR + Oxidation (M)
2121 712.8655 1423.7164 1423.6904 18.3 1 13 0.89 +1Score > 41 indicates identity
Score > 25 indicates homology		 INEIYSGCLILE + Deamidated (NQ)
2912 456.9960 1823.9549 1823.9894 -18.9 1 13 0.84 +1Score > 38 indicates identity
Score > 25 indicates homology		 VTPEGHLATTPHPAVLGK
2977 623.3440 1867.0102 1866.9774 17.5 1 13 0.45 +1Score > 36 indicates identity
Score > 23 indicates homology		 RDIVVWGAMIAGLAHNK + Deamidated (NQ); Oxidation (M)
1567 391.8618 1172.5636 1172.5462 14.8 0 13 0.51 +1Score > 41 indicates identity
Score > 23 indicates homology		 GAANALGGDIWE
2730 860.4621 1718.9096 1718.9427 -19.3 1 13 0.45 +1Score > 39 indicates identity
Score > 23 indicates homology		 LKGLSLDVPNLSGNHR
695 395.7062 789.3978 789.3981 -0.28 0 13 0.85 +1Score > 42 indicates identity
Score > 25 indicates homology		 TSVQQAR + Deamidated (NQ)
1626 600.3403 1198.6660 1198.6710 -4.12 0 13 0.87 +1Score > 40 indicates identity
Score > 25 indicates homology		 HNFLDLILSK
1851 432.9164 1295.7274 1295.7193 6.27 1 13 0.7 +1Score > 37 indicates identity
Score > 24 indicates homology		 MSMLSTLLLKK + 2 Oxidation (M)
2150 722.4122 1442.8098 1442.7841 17.8 1 13 0.38 +1Score > 37 indicates identity
Score > 22 indicates homology		 GNQAIQRSIDTIK
1064 319.8391 956.4955 956.5080 -13.1 1 13 0.61 +1Score > 39 indicates identity
Score > 24 indicates homology		 WVTPEVAR
1010 313.8357 938.4853 938.4934 -8.62 1 13 0.62 +1Score > 39 indicates identity
Score > 24 indicates homology		 ITHNEGLR
2783 877.4650 1752.9154 1752.8902 14.4 1 13 0.93 +1Score > 39 indicates identity
Score > 26 indicates homology		 ILLENGMPVTAHLCGK + Deamidated (NQ)
2351 515.5804 1543.7194 1543.7227 -2.13 0 13 0.47 +1Score > 39 indicates identity
Score > 23 indicates homology		 QQGTSVAQSGAQAPGR + 2 Deamidated (NQ)
2591 825.9327 1649.8508 1649.8624 -7.03 0 13 0.29 +1Score > 40 indicates identity
Score > 21 indicates homology		 ITTVQAAIDFIQSSR + Deamidated (NQ)
2593 825.9330 1649.8514 1649.8301 13.0 0 13 0.65 +1Score > 40 indicates identity
Score > 24 indicates homology		 LGFDVNDDIAAPYIK
3567 560.5400 2238.1309 2238.1453 -6.45 0 13 0.62 +1Score > 39 indicates identity
Score > 24 indicates homology		 NGIHILDLTQTVPMLDQALK + 3 Deamidated (NQ); Oxidation (M)
1679 408.1898 1221.5476 1221.5659 -15.0 1 13 0.72 +1Score > 40 indicates identity
Score > 24 indicates homology		 QMATTLETGDR
3765 794.1033 2379.2881 2379.2885 -0.20 0 13 0.18 +1Score > 34 indicates identity
Score > 18 indicates homology		 FGIGADGVLFLAKPLHTSLHMR
2870 451.4587 1801.8057 1801.8006 2.84 1 13 0.46 +1Score > 38 indicates identity
Score > 23 indicates homology		 ENQTSIYYLAGQDAAR + 3 Deamidated (NQ)
709 399.2320 796.4494 796.4443 6.46 0 13 0.52 +1Score > 36 indicates identity
Score > 23 indicates homology		 GAPAVTPGK
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