MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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91| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS3_PSEE4 | 50 | 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1 |
92| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPX_VARPS | 50 | ATP-dependent Clp protease ATP-binding subunit clpX OS=Variovorax paradoxus (strain S110) GN=clpX PE=3 SV=1 |
93| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPD_PSETO | 49 | Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1 |
94| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ISPD_ALKMQ | 49 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1 |
95| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAB_MYCPN | 48 | Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1 |
96| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | STHA_PSEAB | 47 | Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sthA PE=3 SV=1 |
97| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CYOB_PSEPU | 47 | Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1 |
98| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ASPA_PSEAE | 47 | Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1 |
99| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LEUC_PSEF5 | 45 | 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 99.1 | LEUC_PSEF5 |
45 | 51036 | 4 (1) | 2 (1) | 0.04 | |
| 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1 | |||||||
4 peptide matches (3 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 402 |
322.2112 | 642.4078 | 642.4064 | 2.20 | 0 | 14 | 1.4 | 3Score > 42 indicates identity Score > 28 indicates homology |
U | R.AAAVIAK.G | |
| 405 |
1 |
643.4160 | 642.4087 | 642.4064 | 3.57 | 0 | 45 | 0.025 | 1Score > 41 indicates identity |
U | R.AAAVIAK.G |
| 3556 |
746.3904 | 2236.1494 | 2236.1521 | -1.23 | 1 | 8 | 0.92 | 1Score > 38 indicates identity Score > 20 indicates homology |
U | R.ALKYMGLSANQAITDIQLDR.V + Oxidation (M) |
100| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PANB_STRAW | 44 | 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Streptomyces avermitilis GN=panB PE=3 SV=1 |
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