MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 182)


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+91

Accession Score Description
1 RS3_PSEE4 50 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1

+92

Accession Score Description
1 CLPX_VARPS 50 ATP-dependent Clp protease ATP-binding subunit clpX OS=Variovorax paradoxus (strain S110) GN=clpX PE=3 SV=1

+93

Accession Score Description
1 GLPD_PSETO 49 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

-94

Accession Score Description
1 ISPD_ALKMQ 49 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1
Score Mass Matches Sequences emPAI
94.1 ISPD_ALKMQ 49 27118 1 (1) 1 (1) 0.09
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1
5 samesets of ISPD_ALKMQ
QCRB_STRCO 49 60961 1 (1) 1 (1) 0.04
Ubiquinol-cytochrome c reductase cytochrome b subunit OS=Streptomyces coelicolor GN=qcrB PE=3 SV=1
SYI_MYCAP 49 103127 1 (1) 1 (1) 0.02
Isoleucyl-tRNA synthetase OS=Mycoplasma agalactiae (strain PG2) GN=ileS PE=3 SV=1
HCT1_CHLPN 49 13331 1 (1) 1 (1) 0.18
Histone H1-like protein HC1 OS=Chlamydia pneumoniae GN=hctA PE=2 SV=1
CSRN3_HUMAN 49 66000 1 (1) 1 (1) 0.03
Cysteine/serine-rich nuclear protein 3 OS=Homo sapiens GN=CSRNP3 PE=1 SV=1
RS19E_METTH 49 16608 1 (1) 1 (1) 0.14
30S ribosomal protein S19e OS=Methanobacterium thermoautotrophicum GN=rps19e PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
461   337.2163 672.4180 672.4282 -15.2 1 49 0.0082 +1Score > 40 indicates identity U R.SGILKR.A

+95

Accession Score Description
1 DNAB_MYCPN 48 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+96

Accession Score Description
1 STHA_PSEAB 47 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sthA PE=3 SV=1

+97

Accession Score Description
1 CYOB_PSEPU 47 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1

+98

Accession Score Description
1 ASPA_PSEAE 47 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

+99

Accession Score Description
1 LEUC_PSEF5 45 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1

+100

Accession Score Description
1 PANB_STRAW 44 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Streptomyces avermitilis GN=panB PE=3 SV=1
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