MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 182)

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-91

Accession Score Description
1 RS3_PSEE4 50 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
Score Mass Matches Sequences emPAI
91.1 RS3_PSEE4 50 25709 17 (1) 9 (1) 0.09
30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
3 samesets of RS3_PSEE4
RS3_PSEP1 50 25709 17 (1) 9 (1) 0.09
30S ribosomal protein S3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsC PE=3 SV=1
RS3_PSEPK 50 25709 17 (1) 9 (1) 0.09
30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2
RS3_PSEU5 50 25730 17 (1) 9 (1) 0.09
30S ribosomal protein S3 OS=Pseudomonas stutzeri (strain A1501) GN=rpsC PE=3 SV=1

-17 peptide matches (11 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
428 +1 330.1966 658.3786 658.3762 3.69 0 32 0.24 +1Score > 42 indicates identity
Score > 38 indicates homology		 K.IQVSGR.L
500 +1 346.7130 691.4114 691.4057 8.27 0 18 0.23 +1Score > 37 indicates identity
Score > 25 indicates homology		 K.VWIFK.G
618   377.6806 753.3466 753.3446 2.75 1 20 0.51 +1Score > 39 indicates identity
Score > 29 indicates homology		 R.TEWYR.E
687 +1 393.7283 785.4420 785.4395 3.21 1 35 0.26 +1Score > 43 indicates identity
Score > 42 indicates homology		 R.LGGAEIAR.T
705   398.1996 794.3846 794.3810 4.58 1 7 2.5 +3Score > 41 indicates identity
Score > 24 indicates homology		 R.EYLQDK.L
778   418.2626 834.5106 834.5076 3.69 0 17 1.3 +2Score > 33 indicates identity
Score > 31 indicates homology		 R.VPLHTLR.A
1805   320.1831 1276.7033 1276.7000 2.58 0 13 0.67 +1Score > 38 indicates identity
Score > 24 indicates homology		 R.IDIHRPAQTAR.I
1807 +1 426.5766 1276.7080 1276.7000 6.25 0 18 0.083 +1Score > 37 indicates identity
Score > 20 indicates homology		 R.IDIHRPAQTAR.I
2213 +2 369.7334 1474.9045 1474.8984 4.15 0 7 1 +1Score > 26 indicates identity
Score > 20 indicates homology		 R.ITIHTARPGIVIGK.K
2214   492.6428 1474.9066 1474.8984 5.56 0 17 0.12 +1Score > 25 indicates identity
Score > 20 indicates homology		 R.ITIHTARPGIVIGK.K
3545   744.3678 2230.0816 2230.0430 17.3 1 50 2.3e-005 +1Score > 39 indicates identity
Score > 17 indicates homology		 U R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)

+92

Accession Score Description
1 CLPX_VARPS 50 ATP-dependent Clp protease ATP-binding subunit clpX OS=Variovorax paradoxus (strain S110) GN=clpX PE=3 SV=1

+93

Accession Score Description
1 GLPD_PSETO 49 Glycerol-3-phosphate dehydrogenase OS=Pseudomonas tolaasii GN=glpD PE=3 SV=1

+94

Accession Score Description
1 ISPD_ALKMQ 49 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1

+95

Accession Score Description
1 DNAB_MYCPN 48 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+96

Accession Score Description
1 STHA_PSEAB 47 Soluble pyridine nucleotide transhydrogenase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sthA PE=3 SV=1

+97

Accession Score Description
1 CYOB_PSEPU 47 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1

+98

Accession Score Description
1 ASPA_PSEAE 47 Aspartate ammonia-lyase OS=Pseudomonas aeruginosa GN=aspA PE=3 SV=1

+99

Accession Score Description
1 LEUC_PSEF5 45 3-isopropylmalate dehydratase large subunit OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=leuC PE=3 SV=1

+100

Accession Score Description
1 PANB_STRAW 44 3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Streptomyces avermitilis GN=panB PE=3 SV=1
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