MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 182)


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+81

Accession Score Description
1 IDH_AZOVI 59 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+82

Accession Score Description
1 RL28_PSEE4 58 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1

+83

Accession Score Description
1 SUCC_COXBN 58 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1

+84

Accession Score Description
1 CLPB_PSEPK 57 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+85

Accession Score Description
1 RDRP_ACLSA 57 RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1

+86

Accession Score Description
1 ATPE_PSEPG 57 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+87

Accession Score Description
1 PUR5_PSEMY 55 Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1

+88

Accession Score Description
1 RS9_PSEE4 54 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

-89

Accession Score Description
1 ARGJ_OCEIH 53 Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1
Score Mass Matches Sequences emPAI
89.1 ARGJ_OCEIH 53 44036 2 (2) 1 (1) 0.05
Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1

-2 peptide matches (1 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
736 +1 407.2788 812.5430 812.5371 7.29 1 47 0.0012 +1Score > 31 indicates identity U K.LIEVIVK.G

+90

Accession Score Description
1 RL23_PSEE4 53 50S ribosomal protein L23 OS=Pseudomonas entomophila (strain L48) GN=rplW PE=3 SV=1
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