MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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81| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IDH_AZOVI | 59 | Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5 |
82| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL28_PSEE4 | 58 | 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1 |
83| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_COXBN | 58 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Coxiella burnetii (strain Dugway 5J108-111) GN=sucC PE=3 SV=1 |
84| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 57 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
85| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RDRP_ACLSA | 57 | RNA-directed RNA polymerase OS=Apple chlorotic leaf spot virus (isolate apple) PE=4 SV=1 |
86| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPE_PSEPG | 57 | ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1 |
87| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PUR5_PSEMY | 55 | Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 87.1 | PUR5_PSEMY |
55 | 37182 | 2 (2) | 2 (2) | 0.13 | |
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas mendocina (strain ymp) GN=purM PE=3 SV=1 | |||||||
| 5 samesets of PUR5_PSEMY | |||||||
| PUR5_PSEP1 |
55 | 37375 | 2 (2) | 2 (2) | 0.13 | ||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=purM PE=3 SV=1 | |||||||
| PUR5_PSEPF |
55 | 37293 | 2 (2) | 2 (2) | 0.13 | ||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain Pf0-1) GN=purM PE=3 SV=1 | |||||||
| PUR5_PSEPG |
55 | 37375 | 2 (2) | 2 (2) | 0.13 | ||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain GB-1) GN=purM PE=3 SV=1 | |||||||
| PUR5_PSEPK |
55 | 37375 | 2 (2) | 2 (2) | 0.13 | ||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain KT2440) GN=purM PE=3 SV=1 | |||||||
| PUR5_PSEPW |
55 | 37493 | 2 (2) | 2 (2) | 0.13 | ||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas putida (strain W619) GN=purM PE=3 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1922 |
443.3002 | 1326.8788 | 1326.9002 | -16.2 | 1 | 34 | 0.00059 | 1Score > 14 indicates identity |
U | R.IYVKPLLKLIK.D | |
| 3784 |
799.7648 | 2396.2726 | 2396.2336 | 16.3 | 0 | 43 | 0.0027 | 1Score > 36 indicates identity Score > 30 indicates homology |
U | K.VATGDALIALPSSGPHSNGYSLIR.K + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| PUR5_PSE14 |
34 | 37357 | 87.1 | ||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=purM PE=3 SV=1 | |||||
| 8 samesets of PUR5_PSE14 | |||||
| PUR5_PSEA8 |
34 | 37670 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas aeruginosa (strain LESB58) GN=purM PE=3 SV=1 | |||||
| PUR5_PSEAB |
34 | 37670 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=purM PE=3 SV=1 | |||||
| PUR5_PSEAE |
34 | 37612 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas aeruginosa GN=purM PE=3 SV=1 | |||||
| PUR5_PSEE4 |
34 | 37486 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas entomophila (strain L48) GN=purM PE=3 SV=1 | |||||
| PUR5_PSEF5 |
34 | 37377 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=purM PE=3 SV=1 | |||||
| PUR5_PSEFS |
34 | 37406 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas fluorescens (strain SBW25) GN=purM PE=3 SV=1 | |||||
| PUR5_PSESM |
34 | 37414 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas syringae pv. tomato GN=purM PE=3 SV=1 | |||||
| PUR5_PSEU2 |
34 | 37355 | |||
| Phosphoribosylformylglycinamidine cyclo-ligase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=purM PE=3 SV=1 | |||||
88| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS9_PSEE4 | 54 | 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1 |
89| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARGJ_OCEIH | 53 | Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1 |
90| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL23_PSEE4 | 53 | 50S ribosomal protein L23 OS=Pseudomonas entomophila (strain L48) GN=rplW PE=3 SV=1 |
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