MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 182)

Page: Previous 1 …3 4 5 6 7 8 9 10 11 12 13 … 19 Next 

+71

Accession Score Description
1 ATPG_PSEPG 70 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+72

Accession Score Description
1 HYDA_PSEAE 69 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1

+73

Accession Score Description
1 RL10_PSEPG 69 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+74

Accession Score Description
1 RL15_PSEP1 67 50S ribosomal protein L15 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplO PE=3 SV=1

+75

Accession Score Description
1 RL14_AZOSE 61 50S ribosomal protein L14 OS=Azoarcus sp. (strain EbN1) GN=rplN PE=3 SV=1

+76

Accession Score Description
1 ODP1_MYCTU 66 Pyruvate dehydrogenase E1 component OS=Mycobacterium tuberculosis GN=aceE PE=3 SV=1

+77

Accession Score Description
1 PURA_PSEPG 64 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1

-78

Accession Score Description
1 SYD_AZOVD 62 Aspartyl-tRNA synthetase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=aspS PE=3 SV=1
Score Mass Matches Sequences emPAI
78.1 SYD_AZOVD 62 66329 4 (2) 4 (2) 0.07
Aspartyl-tRNA synthetase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=aspS PE=3 SV=1
5 samesets of SYD_AZOVD
SYD_PSEAB 62 66451 4 (2) 4 (2) 0.07
Aspartyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=aspS PE=3 SV=1
SYD_PSEAE 62 66451 4 (2) 4 (2) 0.07
Aspartyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=aspS PE=3 SV=2
SYD_PSEPK 62 66925 4 (2) 4 (2) 0.07
Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=aspS PE=3 SV=1
SYD_PSEP1 62 66925 4 (2) 4 (2) 0.07
Aspartyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=aspS PE=3 SV=1
SYD_PSEA8 62 66451 4 (2) 4 (2) 0.07
Aspartyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain LESB58) GN=aspS PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
428   330.1966 658.3786 658.3762 3.69 0 24 1.4 +2Score > 42 indicates identity
Score > 38 indicates homology		 E.LGGGSIR.I
1228   512.3038 1022.5930 1022.5801 12.7 0 44 0.0083 +1Score > 36 indicates identity U K.FGFLLDALK.Y
2163   726.9303 1451.8460 1451.8235 15.5 1 53 0.00062 +1Score > 33 indicates identity U R.IPLELVDVADQLK.A
2275   755.3900 1508.7654 1508.7511 9.48 0 10 0.79 +1Score > 41 indicates identity
Score > 21 indicates homology		 U R.VGAVDGDIVFFGADK.A

1 subset or intersection (122 subset proteins in total)

Score Mass Subset of
SYD_ACTP2 44 66995 78.1
Aspartyl-tRNA synthetase OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=aspS PE=3 SV=1
+121 samesets of SYD_ACTP2

+79

Accession Score Description
1 GLNA_AZOVI 62 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+80

Accession Score Description
1 CH10_PSE14 61 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1
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